polyclonal rabbit antibodies Search Results


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    Normal Rabbit IgG rabbit polyclonal antibody Purified
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    Thermo Fisher gfp tag polyclonal antibody
    Gfp Tag Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8757 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rabbit polyclonal antibody
    Rabbit Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 11446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rabbit polyclonal antibody
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    Millipore polyclonal antibodies
    Polyclonal Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rabbit polyclonal antibody
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    Anti Histone H3 Antibody Nuclear Loading Control And Chip Grade, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rabbit polyclonal antibodies
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    Abcam anti histone h3 acetyl k27 antibody chip grade
    Anti Histone H3 Acetyl K27 Antibody Chip Grade, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti cd31 antibody
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Anti Cd31 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti flag antibody
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Anti Flag Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies rabbit polyclonal antibody
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Rabbit Polyclonal Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rabbit polyclonal
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Rabbit Polyclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa rabbit anti dsred
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Rabbit Anti Dsred, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems polyclonal antibody
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 797 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti actin antibody
    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + <t>CD31</t> + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value
    Anti Actin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti active caspase 3 antibody
    LncRNA PART1 regulated cell growth, apoptosis and ECM-related genes through targeting miR-93-5p. (A) Images of NP cell colonies. The colony formation rates are shown on the right. (B) The apoptosis of NP cells was measured by flow cytometry. The apoptosis rates of NP cells are shown on the right. (C and D) The expression levels of Ki67 and <t>C-caspase-3</t> in NP cells were measured by western blotting. (E-G) The expression levels of aggrecan, ADAMTS4, collagen II and MMP13 in NP cells were measured by (E and F) western blotting and (G) reverse transcription-quantitative PCR analysis. ** P
    Anti Active Caspase 3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti nf kb p65 antibody
    Synergistic inhibitory effects of AC and the NF-κB signaling pathway inhibitor JSH-23 on colorectal cancer cells. (A) CCK-8 assay was performed to detect the viability of HCT116 cells treated with AC and JSH-23 for 24, 48 and 72 h. (B) Cell cycle of HCT116 cells treated with AC and JSH-23 for 24 h was assessed using flow cytometry. (C) Statistical analysis of cell cycle of HCT116 cells co-treated with AC and JSH-23 for 24 h. (D) Cell cycle of HCT116 cells co-treated with AC and JSH-23 for 72 h was measured by flow cytometry. (E) Quantification of cell cycle of HCT116 cells co-treated with AC and JSH-23 for 72 h. (F) Cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 24 h was determined using flow cytometry. (G) Cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 72 h was detected by flow cytometry. (H) Statistical analysis of cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 24 h. (I) Statistical analysis of cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 72 h. (J) The phosphorylation of IκBα and <t>P65</t> was measured by western blotting. Data are expressed as mean ± standard deviation from three independent experiments. * P
    Anti Nf Kb P65 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Rabbit Polyclonal CLPH Antibody
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    RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + CD31 + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value

    Journal: Scientific Reports

    Article Title: Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy

    doi: 10.1038/s41598-020-63671-8

    Figure Lengend Snippet: RNA sequencing (RNA-seq) assay shows mesenchymal-endothelial transition (MEndoT)-derived cells are more endothelial-like after cardiac hypertrophy but may be a different cell sub-population. Non-myocytes were isolated from hearts of 14 day post-sham or -transverse aortic constriction (TAC) in Col1a2-CreERT: R26R tdTomato mice and native fibroblasts (tdTomato + -labeled fibroblasts after sham-injury), MEndoT-derived cells (tdTomato + CD31 + -labeled cells) were collected using flow cytometry for RNA-seq. The tdTomato + -labeled endothelial cells from heart tissue of Tek-CreERT: R26R tdTomato mice after sham injury were collected and served as native coronary endothelial cells. ( a ) Principal component analysis clustering show MEndoT-derived cells are a subset cell population with characteristics that were closer to native endothelial cells than native fibroblasts. ( b ) p53 is specifically and significantly upregulated in MEndoT-derived cells. Heatmap show significantly expressed genes indicative of ( c ) fibroblast lineage commitment and ( d ) endothelial lineage commitment (all genes in b-d have a q-value

    Article Snippet: Immunofluorescent staining of frozen sections (7 µm) was performed using primary antibodies to vascular endothelial cadherin (VECAD, ab33168), CD31 (ab28364), von Willebrand factor (vWF, ab11713), endothelial nitric oxide synthase (eNOS, ab5589), occludin (ab31721, all from Abcam, Cambridge, UK), Tek (AF762, R & D Systems, Minnesota, MN, USA), isolectin B4 (B-1205, Vector Labs, Burlingame, CA, USA), p53 (ab31333, Abcam), and associated fluorescein-conjugated secondary antibodies following the manufacturer instructions.

    Techniques: RNA Sequencing Assay, Derivative Assay, Isolation, Mouse Assay, Labeling, Flow Cytometry

    Expression of endothelial markers in mesenchymal-endothelial transition (MEndoT)-derived cells in the heart tissue of Col1a2-CreERT: R26R tdTomato mice after cardiac hypertrophy. ( a-d ) Immunofluorescence staining for endothelial cell markers in heart tissue of Col1a2-CreERT: R26R tdTomato mice after transverse aortic constriction (TAC). ( a ) VECAD expression and the percentage of tdTomato labeled cells expressing VECAD. ( b ) Tek expression and the percentage of tdTomato labeled cells expressing Tek. ( c ) Isolectin B4 expression and the percentage of tdTomato labeled cells expressing isolectin B4. ( d ) CD31 expression and the percentage of tdTomato labeled cells expressing CD31. ( e ) Flow cytometry of isolectin B4 14 days post sham or TAC. (All graphs show mean ± S.E.M; n = 3 animals/group, * p

    Journal: Scientific Reports

    Article Title: Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy

    doi: 10.1038/s41598-020-63671-8

    Figure Lengend Snippet: Expression of endothelial markers in mesenchymal-endothelial transition (MEndoT)-derived cells in the heart tissue of Col1a2-CreERT: R26R tdTomato mice after cardiac hypertrophy. ( a-d ) Immunofluorescence staining for endothelial cell markers in heart tissue of Col1a2-CreERT: R26R tdTomato mice after transverse aortic constriction (TAC). ( a ) VECAD expression and the percentage of tdTomato labeled cells expressing VECAD. ( b ) Tek expression and the percentage of tdTomato labeled cells expressing Tek. ( c ) Isolectin B4 expression and the percentage of tdTomato labeled cells expressing isolectin B4. ( d ) CD31 expression and the percentage of tdTomato labeled cells expressing CD31. ( e ) Flow cytometry of isolectin B4 14 days post sham or TAC. (All graphs show mean ± S.E.M; n = 3 animals/group, * p

    Article Snippet: Immunofluorescent staining of frozen sections (7 µm) was performed using primary antibodies to vascular endothelial cadherin (VECAD, ab33168), CD31 (ab28364), von Willebrand factor (vWF, ab11713), endothelial nitric oxide synthase (eNOS, ab5589), occludin (ab31721, all from Abcam, Cambridge, UK), Tek (AF762, R & D Systems, Minnesota, MN, USA), isolectin B4 (B-1205, Vector Labs, Burlingame, CA, USA), p53 (ab31333, Abcam), and associated fluorescein-conjugated secondary antibodies following the manufacturer instructions.

    Techniques: Expressing, Derivative Assay, Mouse Assay, Immunofluorescence, Staining, Labeling, Flow Cytometry

    LncRNA PART1 regulated cell growth, apoptosis and ECM-related genes through targeting miR-93-5p. (A) Images of NP cell colonies. The colony formation rates are shown on the right. (B) The apoptosis of NP cells was measured by flow cytometry. The apoptosis rates of NP cells are shown on the right. (C and D) The expression levels of Ki67 and C-caspase-3 in NP cells were measured by western blotting. (E-G) The expression levels of aggrecan, ADAMTS4, collagen II and MMP13 in NP cells were measured by (E and F) western blotting and (G) reverse transcription-quantitative PCR analysis. ** P

    Journal: International Journal of Molecular Medicine

    Article Title: Long non-coding RNA PART1 promotes intervertebral disc degeneration through regulating the miR-93/MMP2 pathway in nucleus pulposus cells

    doi: 10.3892/ijmm.2020.4580

    Figure Lengend Snippet: LncRNA PART1 regulated cell growth, apoptosis and ECM-related genes through targeting miR-93-5p. (A) Images of NP cell colonies. The colony formation rates are shown on the right. (B) The apoptosis of NP cells was measured by flow cytometry. The apoptosis rates of NP cells are shown on the right. (C and D) The expression levels of Ki67 and C-caspase-3 in NP cells were measured by western blotting. (E-G) The expression levels of aggrecan, ADAMTS4, collagen II and MMP13 in NP cells were measured by (E and F) western blotting and (G) reverse transcription-quantitative PCR analysis. ** P

    Article Snippet: The membrane was then blocked by 5% non-fat milk (PA201-01, BioMed) for 10 min at room temperature and then incubated with the primary antibodies: Anti-Ki-67 (1:5,000, ab92742, Abcam), anti-cleaved (C)-caspase-3 (1:500, ab2302, Abcam), anti-aggrecan (1:100, ab3778, Abcam), anti-ADAMTS4 (1:1,000, ab185722, Abcam), anti-collagen II (1:2,000, ab34712, Abcam), anti-MMP13 (1:3,000, ab39012, Abcam) and anti-GAPDH (1:2,000, ab8245, Abcam) at 4°C overnight.

    Techniques: Flow Cytometry, Expressing, Western Blot, Real-time Polymerase Chain Reaction

    Synergistic inhibitory effects of AC and the NF-κB signaling pathway inhibitor JSH-23 on colorectal cancer cells. (A) CCK-8 assay was performed to detect the viability of HCT116 cells treated with AC and JSH-23 for 24, 48 and 72 h. (B) Cell cycle of HCT116 cells treated with AC and JSH-23 for 24 h was assessed using flow cytometry. (C) Statistical analysis of cell cycle of HCT116 cells co-treated with AC and JSH-23 for 24 h. (D) Cell cycle of HCT116 cells co-treated with AC and JSH-23 for 72 h was measured by flow cytometry. (E) Quantification of cell cycle of HCT116 cells co-treated with AC and JSH-23 for 72 h. (F) Cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 24 h was determined using flow cytometry. (G) Cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 72 h was detected by flow cytometry. (H) Statistical analysis of cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 24 h. (I) Statistical analysis of cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 72 h. (J) The phosphorylation of IκBα and P65 was measured by western blotting. Data are expressed as mean ± standard deviation from three independent experiments. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Asiaticoside suppresses cell proliferation by inhibiting the NF-κB signaling pathway in colorectal cancer

    doi: 10.3892/ijmm.2020.4688

    Figure Lengend Snippet: Synergistic inhibitory effects of AC and the NF-κB signaling pathway inhibitor JSH-23 on colorectal cancer cells. (A) CCK-8 assay was performed to detect the viability of HCT116 cells treated with AC and JSH-23 for 24, 48 and 72 h. (B) Cell cycle of HCT116 cells treated with AC and JSH-23 for 24 h was assessed using flow cytometry. (C) Statistical analysis of cell cycle of HCT116 cells co-treated with AC and JSH-23 for 24 h. (D) Cell cycle of HCT116 cells co-treated with AC and JSH-23 for 72 h was measured by flow cytometry. (E) Quantification of cell cycle of HCT116 cells co-treated with AC and JSH-23 for 72 h. (F) Cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 24 h was determined using flow cytometry. (G) Cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 72 h was detected by flow cytometry. (H) Statistical analysis of cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 24 h. (I) Statistical analysis of cell apoptosis of HCT116 cells co-treated with AC and JSH-23 for 72 h. (J) The phosphorylation of IκBα and P65 was measured by western blotting. Data are expressed as mean ± standard deviation from three independent experiments. * P

    Article Snippet: The following primary antibodies were used for incubation: Anti-NF-kB P65 (1:1,000; cat. no. ab16502; Abcam), anti-phosphorylated (p)-P65 (1:1,500; cat. no. ab86299; Abcam), anti-caspase-3 (1:1,000; cat. no. ab4051, Abcam), anti-cleaved caspase-3 (1:1,000; cat. no. ab32042; Abcam), anti-caspase-9 (1:1,000; cat. no. ab32539; Abcam), anti-cleaved caspase-9 (1:1,000; cat. no. ab32539; Abcam), anti-Bcl-2 (1:1,000; cat. no. ab32124; Abcam), anti-Bax (1:1,000; cat. no. ab32503; Abcam), anti-CDK4 (1:1,000; cat. no. ab199728, Abcam), anti-Cyclin D1 (1:2,000; cat. no. ab205718; Abcam), anti-IκBα (1:1,000; cat. no. 9242; Cell Signaling Technology, Inc.), anti-p-IκBα (1:1,000; cat. no. 2859; Cell Signaling Technology, Inc.), anti-Histone H3 (1:3,000; cat. no. ab1791; Abcam) and anti-β-actin (1:5,000; cat. no. ab8227; Abcam).

    Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, Standard Deviation

    Effect of AC on the NF-κB signaling pathway. (A) mRNA and (B) protein expression levels of the NF-κB signaling pathway-related molecules IκBα, p-IκBα, P65 and p-P65 in HCT116 cells treated with AC for 72 h. (C) Quantification of the western blots. (D) Nuclear translocation of P65 detected by western blotting in HCT116 cells treated with 2 µ M AC for 72 h. (E) Quantification of the western blots. Data are expressed as mean ± standard deviation from three independent experiments. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Asiaticoside suppresses cell proliferation by inhibiting the NF-κB signaling pathway in colorectal cancer

    doi: 10.3892/ijmm.2020.4688

    Figure Lengend Snippet: Effect of AC on the NF-κB signaling pathway. (A) mRNA and (B) protein expression levels of the NF-κB signaling pathway-related molecules IκBα, p-IκBα, P65 and p-P65 in HCT116 cells treated with AC for 72 h. (C) Quantification of the western blots. (D) Nuclear translocation of P65 detected by western blotting in HCT116 cells treated with 2 µ M AC for 72 h. (E) Quantification of the western blots. Data are expressed as mean ± standard deviation from three independent experiments. * P

    Article Snippet: The following primary antibodies were used for incubation: Anti-NF-kB P65 (1:1,000; cat. no. ab16502; Abcam), anti-phosphorylated (p)-P65 (1:1,500; cat. no. ab86299; Abcam), anti-caspase-3 (1:1,000; cat. no. ab4051, Abcam), anti-cleaved caspase-3 (1:1,000; cat. no. ab32042; Abcam), anti-caspase-9 (1:1,000; cat. no. ab32539; Abcam), anti-cleaved caspase-9 (1:1,000; cat. no. ab32539; Abcam), anti-Bcl-2 (1:1,000; cat. no. ab32124; Abcam), anti-Bax (1:1,000; cat. no. ab32503; Abcam), anti-CDK4 (1:1,000; cat. no. ab199728, Abcam), anti-Cyclin D1 (1:2,000; cat. no. ab205718; Abcam), anti-IκBα (1:1,000; cat. no. 9242; Cell Signaling Technology, Inc.), anti-p-IκBα (1:1,000; cat. no. 2859; Cell Signaling Technology, Inc.), anti-Histone H3 (1:3,000; cat. no. ab1791; Abcam) and anti-β-actin (1:5,000; cat. no. ab8227; Abcam).

    Techniques: Expressing, Western Blot, Translocation Assay, Standard Deviation