polq expression plasmids Search Results


polq expression plasmids  (Addgene inc)
93
Addgene inc polq expression plasmids
a , <t>POLQ</t> mRNA level of POLQ +/R1953X cells increased early in response to DNA damage. b , <t>POLQ</t> <t>expression</t> level increased in POLQ +/R1953X HCT116 cell lines 2h after 6 Gy IR induction compared with POLQ +/+ cells. P -value was determined by two-way analysis of variance. c-d , Flow cytometric analysis of total-NHEJ, TMEJ and HR efficiency showed that POLQ R1953X mutation promoted TMEJ activity in HCT116 ( c ) and 293T ( d ) cells. e , Increased POLQ protein level and decreased DNA damage in POLQ +/R1953X HCT116 cells and 293T cells (f) treated with ultraviolet (UV) or ETOPOSIDE (ETOP), which is time-dependent. VINCILIN, TUBULIN and GADPH were used as the loading control. g-h , Reduced ETOP and UV induced DNA damages in POLQ +/R1953X 293T cells. DNA double-strand breaks measured by γ-H2AX level. Scale bars, 20µm. Data are shown as mean ± SD from at least three independent biological replicates. P -value was determined by two-way ANOVA. * P < 0.05; ** P < 0.01; *** P <0.001; **** P < 0.0001 and ns, not significant. i , Higher somatic mutation burden in cancer tissues derived from POLQ R1953X carriers, with frequently mutated oncogenes shown in ( j ).
Polq Expression Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polq expression plasmids/product/Addgene inc
Average 93 stars, based on 1 article reviews
polq expression plasmids - by Bioz Stars, 2025-11
93/100 stars
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myc and flag-tagged polq expression plasmids  (Addgene inc)
90
Addgene inc myc and flag-tagged polq expression plasmids
a , <t>POLQ</t> mRNA level of POLQ +/R1953X cells increased early in response to DNA damage. b , <t>POLQ</t> <t>expression</t> level increased in POLQ +/R1953X HCT116 cell lines 2h after 6 Gy IR induction compared with POLQ +/+ cells. P -value was determined by two-way analysis of variance. c-d , Flow cytometric analysis of total-NHEJ, TMEJ and HR efficiency showed that POLQ R1953X mutation promoted TMEJ activity in HCT116 ( c ) and 293T ( d ) cells. e , Increased POLQ protein level and decreased DNA damage in POLQ +/R1953X HCT116 cells and 293T cells (f) treated with ultraviolet (UV) or ETOPOSIDE (ETOP), which is time-dependent. VINCILIN, TUBULIN and GADPH were used as the loading control. g-h , Reduced ETOP and UV induced DNA damages in POLQ +/R1953X 293T cells. DNA double-strand breaks measured by γ-H2AX level. Scale bars, 20µm. Data are shown as mean ± SD from at least three independent biological replicates. P -value was determined by two-way ANOVA. * P < 0.05; ** P < 0.01; *** P <0.001; **** P < 0.0001 and ns, not significant. i , Higher somatic mutation burden in cancer tissues derived from POLQ R1953X carriers, with frequently mutated oncogenes shown in ( j ).
Myc And Flag Tagged Polq Expression Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/myc and flag-tagged polq expression plasmids/product/Addgene inc
Average 90 stars, based on 1 article reviews
myc and flag-tagged polq expression plasmids - by Bioz Stars, 2025-11
90/100 stars
  Buy from Supplier

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a , POLQ mRNA level of POLQ +/R1953X cells increased early in response to DNA damage. b , POLQ expression level increased in POLQ +/R1953X HCT116 cell lines 2h after 6 Gy IR induction compared with POLQ +/+ cells. P -value was determined by two-way analysis of variance. c-d , Flow cytometric analysis of total-NHEJ, TMEJ and HR efficiency showed that POLQ R1953X mutation promoted TMEJ activity in HCT116 ( c ) and 293T ( d ) cells. e , Increased POLQ protein level and decreased DNA damage in POLQ +/R1953X HCT116 cells and 293T cells (f) treated with ultraviolet (UV) or ETOPOSIDE (ETOP), which is time-dependent. VINCILIN, TUBULIN and GADPH were used as the loading control. g-h , Reduced ETOP and UV induced DNA damages in POLQ +/R1953X 293T cells. DNA double-strand breaks measured by γ-H2AX level. Scale bars, 20µm. Data are shown as mean ± SD from at least three independent biological replicates. P -value was determined by two-way ANOVA. * P < 0.05; ** P < 0.01; *** P <0.001; **** P < 0.0001 and ns, not significant. i , Higher somatic mutation burden in cancer tissues derived from POLQ R1953X carriers, with frequently mutated oncogenes shown in ( j ).

Journal: medRxiv

Article Title: A germline heterozygous POLQ nonsense mutation causes hereditary colorectal cancer

doi: 10.1101/2024.01.13.23299913

Figure Lengend Snippet: a , POLQ mRNA level of POLQ +/R1953X cells increased early in response to DNA damage. b , POLQ expression level increased in POLQ +/R1953X HCT116 cell lines 2h after 6 Gy IR induction compared with POLQ +/+ cells. P -value was determined by two-way analysis of variance. c-d , Flow cytometric analysis of total-NHEJ, TMEJ and HR efficiency showed that POLQ R1953X mutation promoted TMEJ activity in HCT116 ( c ) and 293T ( d ) cells. e , Increased POLQ protein level and decreased DNA damage in POLQ +/R1953X HCT116 cells and 293T cells (f) treated with ultraviolet (UV) or ETOPOSIDE (ETOP), which is time-dependent. VINCILIN, TUBULIN and GADPH were used as the loading control. g-h , Reduced ETOP and UV induced DNA damages in POLQ +/R1953X 293T cells. DNA double-strand breaks measured by γ-H2AX level. Scale bars, 20µm. Data are shown as mean ± SD from at least three independent biological replicates. P -value was determined by two-way ANOVA. * P < 0.05; ** P < 0.01; *** P <0.001; **** P < 0.0001 and ns, not significant. i , Higher somatic mutation burden in cancer tissues derived from POLQ R1953X carriers, with frequently mutated oncogenes shown in ( j ).

Article Snippet: Myc and Flag-tagged POLQ expression plasmids were purchased from Addgene (#73132).

Techniques: Expressing, Mutagenesis, Activity Assay, Derivative Assay