pnk buffer Search Results


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  • 96
    New England Biolabs pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk buffer/product/New England Biolabs
    Average 96 stars, based on 489 article reviews
    Price from $9.99 to $1999.99
    pnk buffer - by Bioz Stars, 2020-05
    96/100 stars
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    99
    Thermo Fisher pnk buffer a
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk buffer a/product/Thermo Fisher
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    pnk buffer a - by Bioz Stars, 2020-05
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    91
    Promega pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    pnk buffer - by Bioz Stars, 2020-05
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    90
    TaKaRa pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk buffer/product/TaKaRa
    Average 90 stars, based on 5 article reviews
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    91
    Thermo Fisher pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk buffer/product/Thermo Fisher
    Average 91 stars, based on 125 article reviews
    Price from $9.99 to $1999.99
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    93
    Millipore pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk buffer/product/Millipore
    Average 93 stars, based on 5 article reviews
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    85
    Roche 10x pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    10x Pnk Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x pnk buffer/product/Roche
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    10x pnk buffer - by Bioz Stars, 2020-05
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    91
    Roche pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk buffer/product/Roche
    Average 91 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    pnk buffer - by Bioz Stars, 2020-05
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    99
    Thermo Fisher t4 pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    T4 Pnk Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 pnk buffer/product/Thermo Fisher
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    t4 pnk buffer - by Bioz Stars, 2020-05
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    88
    TaKaRa 10x t4 pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    10x T4 Pnk Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher polynucleotide kinase pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Polynucleotide Kinase Pnk Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    polynucleotide kinase pnk buffer - by Bioz Stars, 2020-05
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    99
    Thermo Fisher pnk wash buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    Pnk Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnk wash buffer/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pnk wash buffer - by Bioz Stars, 2020-05
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    90
    Enzymatics t4 pnk buffer
    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). <t>T4</t> RNAP synthesized RNA (264 nt) was kinased using T4 <t>PNK</t> and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.
    T4 Pnk Buffer, supplied by Enzymatics, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 pnk buffer/product/Enzymatics
    Average 90 stars, based on 5 article reviews
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    t4 pnk buffer - by Bioz Stars, 2020-05
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    Image Search Results


    ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). T4 RNAP synthesized RNA (264 nt) was kinased using T4 PNK and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nascent RNA sequencing reveals distinct features in plant transcription

    doi: 10.1073/pnas.1603217113

    Figure Lengend Snippet: ( A ) Effect of enzymes on 5′ monophosporylated (5′Pi) or capped RNA (CAP). T4 RNAP synthesized RNA (264 nt) was kinased using T4 PNK and [α- 32 P]ATP or capped with the Vaccinia Capping System (M2080) and [α- 32 P]GTP, as described by the manufacturer. ( B ) Comparison of RppH activity on 32 P-capped RNA in buffer NEB II vs. NEB T4 RNA ligase buffer. ( C ) 32 P-capped RNA (10 pmol) (264 nt) incubated with 0.5 U of RppH at 37 °C and 20 °C. ( D ) [ 32 P]5′-adenylated oligo (20 pmol) (55 nt) incubated with 2 U of RppH at 20 °C and 37 °C in T4 RNA ligase buffer. ( E ) Assessment of run-on length: nuclei were run on using the described run-on conditions (20 nM CTP-limiting) for the indicated time in the presence and absence of 4 ng/µL α-amanitin, a concentration efficiently inhibiting RNAP II transcription. For visualization of actual run-on length, nuclei were incubated in Freezing Buffer + RNase A (0.25 mg/mL) for 20 min at 4 °C followed by 5 min at RT and consecutively washed three times before run-on.

    Article Snippet: Add kinasing master mix containing 10 µL of PNK buffer (NEB), 1 µL of T4 Polynucleotide Kinase, 5 µL of 10 mM ATP, and dH2O+T for a 100-µL final volume and incubate another 1 h at 37 °C.

    Techniques: Synthesized, Activity Assay, Incubation, Concentration Assay