Journal: Viruses

Article Title: Binding of hnRNP I–vRNA Regulates Sindbis Virus Structural Protein Expression to Promote Particle Infectivity

doi: 10.3390/v14071423

Figure Lengend Snippet: The loss of hnRNP I binding negatively impacts the glycosylation of the viral glycoproteins. ( A ) SINV.WT or SINV.hnRNP I TAR viruses were incubated in the presence or absence of PNGase F overnight at room temperature under nondenaturing conditions. After treatment the viral titer was quantified and the change in viral titer is presented for each pairwise sample. ( B ) The relative impact of deglycosylation, as determined by the average ratio of treated and untreated samples. Quantitative data shown are the means of three independent PNGase F assays, with the error bars representing the standard deviation of the means. Statistical significance as determined by Student’s t-test is indicated above the specific comparisons (with ** ≤ 0.01).

Article Snippet: Viral particles were deglycosylated via treatment with PNGase F (Recombinant; New England Biolabs, Ipswich, MA, USA) under nondenaturing reaction conditions.

Techniques: Binding Assay, Incubation, Standard Deviation