pmd19-t vector Search Results


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  • 99
    TaKaRa pmd19 t vector kit
    Construction of CYP1A1 overexpression plasmids and verification of overexpression efficiency. (a) Cloned full-length CYP1A1 cDNA from MAC-T cells was analyzed using PCR. (b) The dual-enzyme digestion of the plasmid of <t>pMD19-T–CYP1A1</t> cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector. (c) The efficient connection of cloned CYP1A1 cDNAs to the target plasmid was confirmed using plasmid PCR. Control: empty vector. 1, 2, and 3: target vector connected to CYP1A1 from three bacterial colonies. (d) Overexpression efficiency of CYP1A1 was verified using RT-qPCR and Western blot. GAPDH was used as an internal control. Data were expressed as the mean ± SD. ∗ p
    Pmd19 T Vector Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector kit/product/TaKaRa
    Average 99 stars, based on 165 article reviews
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    pmd19 t vector kit - by Bioz Stars, 2020-08
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    93
    Sangon Biotech pmd19 t vector
    Construction of CYP1A1 overexpression plasmids and verification of overexpression efficiency. (a) Cloned full-length CYP1A1 cDNA from MAC-T cells was analyzed using PCR. (b) The dual-enzyme digestion of the plasmid of <t>pMD19-T–CYP1A1</t> cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector. (c) The efficient connection of cloned CYP1A1 cDNAs to the target plasmid was confirmed using plasmid PCR. Control: empty vector. 1, 2, and 3: target vector connected to CYP1A1 from three bacterial colonies. (d) Overexpression efficiency of CYP1A1 was verified using RT-qPCR and Western blot. GAPDH was used as an internal control. Data were expressed as the mean ± SD. ∗ p
    Pmd19 T Vector, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector/product/Sangon Biotech
    Average 93 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    pmd19 t vector - by Bioz Stars, 2020-08
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    92
    Genewiz pmd19 t vector
    Construction of CYP1A1 overexpression plasmids and verification of overexpression efficiency. (a) Cloned full-length CYP1A1 cDNA from MAC-T cells was analyzed using PCR. (b) The dual-enzyme digestion of the plasmid of <t>pMD19-T–CYP1A1</t> cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector. (c) The efficient connection of cloned CYP1A1 cDNAs to the target plasmid was confirmed using plasmid PCR. Control: empty vector. 1, 2, and 3: target vector connected to CYP1A1 from three bacterial colonies. (d) Overexpression efficiency of CYP1A1 was verified using RT-qPCR and Western blot. GAPDH was used as an internal control. Data were expressed as the mean ± SD. ∗ p
    Pmd19 T Vector, supplied by Genewiz, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector/product/Genewiz
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    pmd19 t vector - by Bioz Stars, 2020-08
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    91
    Beijing Genomics Institute Shenzhen pmd19 t vector
    Construction of CYP1A1 overexpression plasmids and verification of overexpression efficiency. (a) Cloned full-length CYP1A1 cDNA from MAC-T cells was analyzed using PCR. (b) The dual-enzyme digestion of the plasmid of <t>pMD19-T–CYP1A1</t> cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector. (c) The efficient connection of cloned CYP1A1 cDNAs to the target plasmid was confirmed using plasmid PCR. Control: empty vector. 1, 2, and 3: target vector connected to CYP1A1 from three bacterial colonies. (d) Overexpression efficiency of CYP1A1 was verified using RT-qPCR and Western blot. GAPDH was used as an internal control. Data were expressed as the mean ± SD. ∗ p
    Pmd19 T Vector, supplied by Beijing Genomics Institute Shenzhen, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector/product/Beijing Genomics Institute Shenzhen
    Average 91 stars, based on 1 article reviews
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    92
    Thermo Fisher pmd19 t vector
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Pmd19 T Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector/product/Thermo Fisher
    Average 92 stars, based on 179 article reviews
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    93
    Vazyme Biotech Co pmd19 t vector
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Pmd19 T Vector, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector/product/Vazyme Biotech Co
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    90
    tiangen biotech co pmd19 t vector
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Pmd19 T Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 5 article reviews
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    93
    Promega pmd19 t vector
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Pmd19 T Vector, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector/product/Promega
    Average 93 stars, based on 16 article reviews
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    84
    TaKaRa pmd19 t vector template
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Pmd19 T Vector Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 t vector template/product/TaKaRa
    Average 84 stars, based on 1 article reviews
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    88
    tiangen biotech co recombinant pmd19 t vector
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Recombinant Pmd19 T Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore pmd 19 t vector
    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the <t>pMD19-T</t> vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.
    Pmd 19 T Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd 19 t vector/product/Millipore
    Average 93 stars, based on 12 article reviews
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    pmd 19 t vector - by Bioz Stars, 2020-08
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    Image Search Results


    Construction of CYP1A1 overexpression plasmids and verification of overexpression efficiency. (a) Cloned full-length CYP1A1 cDNA from MAC-T cells was analyzed using PCR. (b) The dual-enzyme digestion of the plasmid of pMD19-T–CYP1A1 cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector. (c) The efficient connection of cloned CYP1A1 cDNAs to the target plasmid was confirmed using plasmid PCR. Control: empty vector. 1, 2, and 3: target vector connected to CYP1A1 from three bacterial colonies. (d) Overexpression efficiency of CYP1A1 was verified using RT-qPCR and Western blot. GAPDH was used as an internal control. Data were expressed as the mean ± SD. ∗ p

    Journal: Mediators of Inflammation

    Article Title: CYP1A1 Relieves Lipopolysaccharide-Induced Inflammatory Responses in Bovine Mammary Epithelial Cells

    doi: 10.1155/2018/4093285

    Figure Lengend Snippet: Construction of CYP1A1 overexpression plasmids and verification of overexpression efficiency. (a) Cloned full-length CYP1A1 cDNA from MAC-T cells was analyzed using PCR. (b) The dual-enzyme digestion of the plasmid of pMD19-T–CYP1A1 cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector. (c) The efficient connection of cloned CYP1A1 cDNAs to the target plasmid was confirmed using plasmid PCR. Control: empty vector. 1, 2, and 3: target vector connected to CYP1A1 from three bacterial colonies. (d) Overexpression efficiency of CYP1A1 was verified using RT-qPCR and Western blot. GAPDH was used as an internal control. Data were expressed as the mean ± SD. ∗ p

    Article Snippet: The dual-enzyme digestion of the plasmid of pMD19-T–CYP1A1 cDNA confirmed that CYP1A1 cDNA was accurately connected into the pMD19-T vector ( ).

    Techniques: Over Expression, Polymerase Chain Reaction, Plasmid Preparation, Clone Assay, Quantitative RT-PCR, Western Blot

    Sensitivity analysis of the exo-RPA assay. Different copy numbers of plasmid pMD19-T-TK DNA (10 6 to 10 0 copies) were amplified by either RPA reactions or real time PCR. As shown in this figure, the detection limit was 10 2 copies of DNA/reaction for both the exo-RPA assay (panel A) and real time PCR (panel B). The copy numbers used as template for curve 1–7 were 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 and 10 0 , respectively. Shown in this figure is one representative plot out of 5 independent reactions for RPA and real time PCR, respectively.

    Journal: PLoS ONE

    Article Title: Recombinase Polymerase Amplification Assay—A Simple, Fast and Cost-Effective Alternative to Real Time PCR for Specific Detection of Feline Herpesvirus-1

    doi: 10.1371/journal.pone.0166903

    Figure Lengend Snippet: Sensitivity analysis of the exo-RPA assay. Different copy numbers of plasmid pMD19-T-TK DNA (10 6 to 10 0 copies) were amplified by either RPA reactions or real time PCR. As shown in this figure, the detection limit was 10 2 copies of DNA/reaction for both the exo-RPA assay (panel A) and real time PCR (panel B). The copy numbers used as template for curve 1–7 were 10 6 , 10 5 , 10 4 , 10 3 , 10 2 , 10 1 and 10 0 , respectively. Shown in this figure is one representative plot out of 5 independent reactions for RPA and real time PCR, respectively.

    Article Snippet: The TK gene was cloned into plasmid pMD19-T using the pMD19-T Vector Cloning Kit (Takara Bio Inc.) following the manufacturer’s instructions.

    Techniques: Recombinase Polymerase Amplification, Plasmid Preparation, Amplification, Real-time Polymerase Chain Reaction

    Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the pMD19-T vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.

    Journal: The Journal of International Medical Research

    Article Title: Construction and characterization of an adenoviral vector encoding human bone morphogenetic protein-2

    doi: 10.1177/0300060520910320

    Figure Lengend Snippet: Agarose gel electrophoresis showing PCR amplification of human BMP2 and cloning into the pMD19-T vector. (a) PCR product amplified using BMP-2 cloning primers. Lane 1: DL2000 Marker; Lane 2: BMP2 PCR product. (b) Lane 1: DL2000 Marker; Lane 2: double restriction enzyme digestion of recombinant TS-BMP-2 vector.

    Article Snippet: Subcloning of BMP2 into pMD19-T cloning vector The purified PCR product was ligated into the pMD19-T vector (Invitrogen, USA).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Clone Assay, Plasmid Preparation, Marker, Recombinant