pmd18-t cloning vector Search Results


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  • 91
    TaKaRa pmd18 t cloning vector
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Pmd18 T Cloning Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 536 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t cloning vector/product/TaKaRa
    Average 91 stars, based on 536 article reviews
    Price from $9.99 to $1999.99
    pmd18 t cloning vector - by Bioz Stars, 2020-08
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    89
    TaKaRa pmd18 t vector cloning kit
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Pmd18 T Vector Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t vector cloning kit/product/TaKaRa
    Average 89 stars, based on 175 article reviews
    Price from $9.99 to $1999.99
    pmd18 t vector cloning kit - by Bioz Stars, 2020-08
    89/100 stars
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    90
    TaKaRa pmd18 t pcr cloning vector
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Pmd18 T Pcr Cloning Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t pcr cloning vector/product/TaKaRa
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    pmd18 t pcr cloning vector - by Bioz Stars, 2020-08
    90/100 stars
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    85
    Beijing TransGen Biotech pmd18 t cloning vectors
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Pmd18 T Cloning Vectors, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t cloning vectors/product/Beijing TransGen Biotech
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    pmd18 t cloning vectors - by Bioz Stars, 2020-08
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    88
    Thermo Fisher cloning vector pmd18 t
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Cloning Vector Pmd18 T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloning vector pmd18 t/product/Thermo Fisher
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    cloning vector pmd18 t - by Bioz Stars, 2020-08
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    86
    Promega pmd18 t promega cloning vector
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Pmd18 T Promega Cloning Vector, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t promega cloning vector/product/Promega
    Average 86 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pmd18 t promega cloning vector - by Bioz Stars, 2020-08
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    90
    TaKaRa ta cloning vector
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Ta Cloning Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ta cloning vector/product/TaKaRa
    Average 90 stars, based on 42 article reviews
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    85
    TaKaRa pcr cloning vector pmd18t
    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of <t>pMD18-T</t> (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.
    Pcr Cloning Vector Pmd18t, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr cloning vector pmd18t/product/TaKaRa
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
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    92
    TaKaRa pmd18 t
    Standard curves of the Quantitative real-time PCR . Standard curves were constructed using serial dilutions of the ORF segments of the genes recombined into <t>pMD18-T</t> vectors. The log ranges of the standard curves are from 10 6 to 10 2 copies. Threshold cycles of each standard dilution point were used to generate the standard curve for each gene. Linear equations corresponding to each standard curve are given, and R 2 for each standard curve is more than 0.99.
    Pmd18 T, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t/product/TaKaRa
    Average 92 stars, based on 1944 article reviews
    Price from $9.99 to $1999.99
    pmd18 t - by Bioz Stars, 2020-08
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    91
    Thermo Fisher pmd18 t vectors
    Standard curves of the Quantitative real-time PCR . Standard curves were constructed using serial dilutions of the ORF segments of the genes recombined into <t>pMD18-T</t> vectors. The log ranges of the standard curves are from 10 6 to 10 2 copies. Threshold cycles of each standard dilution point were used to generate the standard curve for each gene. Linear equations corresponding to each standard curve are given, and R 2 for each standard curve is more than 0.99.
    Pmd18 T Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t vectors/product/Thermo Fisher
    Average 91 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    pmd18 t vectors - by Bioz Stars, 2020-08
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    95
    TaKaRa pmd18 t vector
    The schematic concepts of duplex real-time PCR with internal standard for quantitative detection of plasma DNA. The whole process includes 4 steps: (A) A 41-bp artificial double-stranded DNA sequence corresponding to the human β–actin gene is cloned and inserted to <t>pMD18-T</t> vector, which is then linearized by restriction enzyme digestion. (B) The recombinant plasmid DNA is added into the cell-free plasma sample with known concentration and extracted together with endogenous nucleic acids. (C) The internal standard and target gene are then amplified simultaneously in the same tube with the common reverse primer focusing on the 41-bp corresponding sequence by duplex real-time PCR, where fluorescence signals are detected separately (Applied Biosystems 7500 Sequence Detector). (D) The plasma DNA concentrations are calculated according to the internal standard by the equation.
    Pmd18 T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 16388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t vector/product/TaKaRa
    Average 95 stars, based on 16388 article reviews
    Price from $9.99 to $1999.99
    pmd18 t vector - by Bioz Stars, 2020-08
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      Buy from Supplier

    93
    Thermo Fisher pmd18 t vector
    The schematic concepts of duplex real-time PCR with internal standard for quantitative detection of plasma DNA. The whole process includes 4 steps: (A) A 41-bp artificial double-stranded DNA sequence corresponding to the human β–actin gene is cloned and inserted to <t>pMD18-T</t> vector, which is then linearized by restriction enzyme digestion. (B) The recombinant plasmid DNA is added into the cell-free plasma sample with known concentration and extracted together with endogenous nucleic acids. (C) The internal standard and target gene are then amplified simultaneously in the same tube with the common reverse primer focusing on the 41-bp corresponding sequence by duplex real-time PCR, where fluorescence signals are detected separately (Applied Biosystems 7500 Sequence Detector). (D) The plasma DNA concentrations are calculated according to the internal standard by the equation.
    Pmd18 T Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd18 t vector/product/Thermo Fisher
    Average 93 stars, based on 265 article reviews
    Price from $9.99 to $1999.99
    pmd18 t vector - by Bioz Stars, 2020-08
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    Image Search Results


    Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of pMD18-T (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.

    Journal: Molecules

    Article Title: Expression, Purification, and Characterization of Cu/ZnSOD from Panax Ginseng

    doi: 10.3390/molecules19068112

    Figure Lengend Snippet: Nucleic acid electrophoresis analysis of the recombinant plasmids. ( a ) Line C, the bands of pMD18-T (2560 bp) and SOD (459 bp). Line M, DL2000 Marker; ( b ) Line D, the bands of pBV220 (3660 bp) and SOD (459 bp). Line M, DL5000 Marker.

    Article Snippet: The cloning vector pMD18-T was purchased from TakaRa, and the expression vector pBV220 was obtained from the Changchun Institute of Biological Products.

    Techniques: Nucleic Acid Electrophoresis, Recombinant, Marker

    Standard curves of the Quantitative real-time PCR . Standard curves were constructed using serial dilutions of the ORF segments of the genes recombined into pMD18-T vectors. The log ranges of the standard curves are from 10 6 to 10 2 copies. Threshold cycles of each standard dilution point were used to generate the standard curve for each gene. Linear equations corresponding to each standard curve are given, and R 2 for each standard curve is more than 0.99.

    Journal: BMC Genetics

    Article Title: Analysis of four achaete-scute homologs in Bombyx mori reveals new viewpoints of the evolution and functions of this gene family

    doi: 10.1186/1471-2156-9-24

    Figure Lengend Snippet: Standard curves of the Quantitative real-time PCR . Standard curves were constructed using serial dilutions of the ORF segments of the genes recombined into pMD18-T vectors. The log ranges of the standard curves are from 10 6 to 10 2 copies. Threshold cycles of each standard dilution point were used to generate the standard curve for each gene. Linear equations corresponding to each standard curve are given, and R 2 for each standard curve is more than 0.99.

    Article Snippet: Standard curves were constructed using serial dilutions of the ORF segments of the genes recombined into pMD18-T vectors (Takara, Japan).

    Techniques: Real-time Polymerase Chain Reaction, Construct

    The schematic concepts of duplex real-time PCR with internal standard for quantitative detection of plasma DNA. The whole process includes 4 steps: (A) A 41-bp artificial double-stranded DNA sequence corresponding to the human β–actin gene is cloned and inserted to pMD18-T vector, which is then linearized by restriction enzyme digestion. (B) The recombinant plasmid DNA is added into the cell-free plasma sample with known concentration and extracted together with endogenous nucleic acids. (C) The internal standard and target gene are then amplified simultaneously in the same tube with the common reverse primer focusing on the 41-bp corresponding sequence by duplex real-time PCR, where fluorescence signals are detected separately (Applied Biosystems 7500 Sequence Detector). (D) The plasma DNA concentrations are calculated according to the internal standard by the equation.

    Journal: Annals of Laboratory Medicine

    Article Title: Development and Evaluation of a Duplex Real-Time PCR Assay With a Novel Internal Standard for Precise Quantification of Plasma DNA

    doi: 10.3343/alm.2017.37.1.18

    Figure Lengend Snippet: The schematic concepts of duplex real-time PCR with internal standard for quantitative detection of plasma DNA. The whole process includes 4 steps: (A) A 41-bp artificial double-stranded DNA sequence corresponding to the human β–actin gene is cloned and inserted to pMD18-T vector, which is then linearized by restriction enzyme digestion. (B) The recombinant plasmid DNA is added into the cell-free plasma sample with known concentration and extracted together with endogenous nucleic acids. (C) The internal standard and target gene are then amplified simultaneously in the same tube with the common reverse primer focusing on the 41-bp corresponding sequence by duplex real-time PCR, where fluorescence signals are detected separately (Applied Biosystems 7500 Sequence Detector). (D) The plasma DNA concentrations are calculated according to the internal standard by the equation.

    Article Snippet: Internal standard A 41-bp artificial dsDNA sequence corresponding to the human β-actin gene was cloned and inserted into the pMD18-T vector using a Takara Reagent Set (Takara, Dalian, China).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing, Clone Assay, Plasmid Preparation, Recombinant, Concentration Assay, Amplification, Fluorescence