pmal-c2 vector New England Biolabs Search Results


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  • 99
    New England Biolabs pmal c2
    Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs pmal c2 vector
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Pmal C2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs xmni linearized pmal c2 vector
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Xmni Linearized Pmal C2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs maltose binding protein fusion vector
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Maltose Binding Protein Fusion Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs pmal c2 vector system
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Pmal C2 Vector System, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs expression vector pmal c2
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Expression Vector Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs xbai digested pmal c2 vector
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Xbai Digested Pmal C2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs xmni hindiii digested vector pmal c2
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Xmni Hindiii Digested Vector Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs male rbo fusion protein
    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified <t>pMal-c2</t> vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
    Male Rbo Fusion Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified pMal-c2 vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.

    Journal: The Journal of parasitology

    Article Title: IDENTIFICATION OF A SPOROZOITE-SPECIFIC ANTIGEN FROM TOXOPLASMA GONDII

    doi: 10.1645/GE-2782.1

    Figure Lengend Snippet: Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified pMal-c2 vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.

    Article Snippet: The identified gene was subcloned into the EcoRI/Hind III site of pMal-c2 vector (New England Biolabs, Beverly, Massachusetts) for constitutive protein expression and was expressed as an N-terminal maltose binding fusion protein under the control of the lac repressor.

    Techniques: Western Blot, Purification, Plasmid Preparation, Infection