Journal: The Journal of parasitology
Article Title: IDENTIFICATION OF A SPOROZOITE-SPECIFIC ANTIGEN FROM TOXOPLASMA GONDII
Figure Lengend Snippet: Western blots were carried out using human sera and the Factor Xa cleaved-fusion protein purified over a DEAE sepharose column (purified TgERP insert). A, Lane 1, Factor Xa-cleaved, DEAE column purified pMal-c2 vector without the TgERP insert. Lanes 2–7, purified insert, probed with individual sera from Group 1, 6 people from laboratory outbreak resulting from oocyst ingestion. Sera collected 6 weeks post-infection. B, Left, purified insert, probed with pooled sera from 6 Group 1 individuals, 12 weeks post infection; Right; same, probed with pooled sera from 4 chronically infected persons from Group 2. C, purified insert, probed with pooled sera from 6 Group 1 individuals, 5 months post infection D, purified insert, Lanes 1–9, probed with sera of 9 people from stable outbreak (Group 2), Lanes 10–12, probed with sera of 3 IgM positive women from Group 3.
Article Snippet: The identified gene was subcloned into the EcoRI/Hind III site of pMal-c2 vector (New England Biolabs, Beverly, Massachusetts) for constitutive protein expression and was expressed as an N-terminal maltose binding fusion protein under the control of the lac repressor.
Techniques: Western Blot, Purification, Plasmid Preparation, Infection