plvx ires zsgreen1 Takara Search Results


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  • 99
    ATCC thp 1 cells
    Knockdown of SLAMF1 in macrophages results in strongly reduced TLR4-mediated IFNβ mRNA expression and protein secretion as well as some decrease of TNF, IL-6, and CXCL10 secretion. (A and B) Quantification of SLAMF1 , IFNβ , and TNF mRNA expression by qPCR in <t>THP-1</t> cells (A) and macrophages (B) treated by 100 ng/ml ultrapure K12 LPS. (C and D) IFNβ and TNF secretion levels by THP-1 cells (C) and macrophages (D) in response to LPS (4 and 6 h) assessed by ELISA. (E and F) Secretion levels of IL-1β, IL-6, IL-8, and CXCL-10 (6 h LPS) analyzed by multiplex assays. Data are presented as means with SD for combined data from three independent experiments (A, C, and E), for three biological replicates from one of six donors (B and D), or one of three donors (F). *, P
    Thp 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore genejuice transfection reagent
    Knockdown of SLAMF1 in macrophages results in strongly reduced TLR4-mediated IFNβ mRNA expression and protein secretion as well as some decrease of TNF, IL-6, and CXCL10 secretion. (A and B) Quantification of SLAMF1 , IFNβ , and TNF mRNA expression by qPCR in <t>THP-1</t> cells (A) and macrophages (B) treated by 100 ng/ml ultrapure K12 LPS. (C and D) IFNβ and TNF secretion levels by THP-1 cells (C) and macrophages (D) in response to LPS (4 and 6 h) assessed by ELISA. (E and F) Secretion levels of IL-1β, IL-6, IL-8, and CXCL-10 (6 h LPS) analyzed by multiplex assays. Data are presented as means with SD for combined data from three independent experiments (A, C, and E), for three biological replicates from one of six donors (B and D), or one of three donors (F). *, P
    Genejuice Transfection Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa plvx ires zsgreen1 vector
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    Plvx Ires Zsgreen1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa ecori
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    Ecori, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 8219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa 293t cells
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    293t Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa in fusion hd cloning kit
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    In Fusion Hd Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa lentiviral expression vector
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    Lentiviral Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    TaKaRa plvx ef1alpha ires zsgreen1 vector
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    Plvx Ef1alpha Ires Zsgreen1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa plvx puro
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    Plvx Puro, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc packaging plasmids
    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the <t>pLVX-IRES-GFP</t> vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.
    Packaging Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 1524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher pgipz
    Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with <t>pLVX</t> ZsGreen1, pLVTHM, <t>pGIPZ</t> and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry. GFP, green fluorescent protein; RFP, red fluorescent protein; HIV, human immunodeficiency virus; SFFV, spleen focus-forming virus.
    Pgipz, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 645 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc plvthm
    Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, <t>pLVTHM,</t> <t>pGIPZ</t> and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry.
    Plvthm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Knockdown of SLAMF1 in macrophages results in strongly reduced TLR4-mediated IFNβ mRNA expression and protein secretion as well as some decrease of TNF, IL-6, and CXCL10 secretion. (A and B) Quantification of SLAMF1 , IFNβ , and TNF mRNA expression by qPCR in THP-1 cells (A) and macrophages (B) treated by 100 ng/ml ultrapure K12 LPS. (C and D) IFNβ and TNF secretion levels by THP-1 cells (C) and macrophages (D) in response to LPS (4 and 6 h) assessed by ELISA. (E and F) Secretion levels of IL-1β, IL-6, IL-8, and CXCL-10 (6 h LPS) analyzed by multiplex assays. Data are presented as means with SD for combined data from three independent experiments (A, C, and E), for three biological replicates from one of six donors (B and D), or one of three donors (F). *, P

    Journal: The Journal of Cell Biology

    Article Title: SLAMF1 is required for TLR4-mediated TRAM-TRIF–dependent signaling in human macrophages

    doi: 10.1083/jcb.201707027

    Figure Lengend Snippet: Knockdown of SLAMF1 in macrophages results in strongly reduced TLR4-mediated IFNβ mRNA expression and protein secretion as well as some decrease of TNF, IL-6, and CXCL10 secretion. (A and B) Quantification of SLAMF1 , IFNβ , and TNF mRNA expression by qPCR in THP-1 cells (A) and macrophages (B) treated by 100 ng/ml ultrapure K12 LPS. (C and D) IFNβ and TNF secretion levels by THP-1 cells (C) and macrophages (D) in response to LPS (4 and 6 h) assessed by ELISA. (E and F) Secretion levels of IL-1β, IL-6, IL-8, and CXCL-10 (6 h LPS) analyzed by multiplex assays. Data are presented as means with SD for combined data from three independent experiments (A, C, and E), for three biological replicates from one of six donors (B and D), or one of three donors (F). *, P

    Article Snippet: Transduced THP-1 cells were selected with puromycin (1 μg/ml) for 1 mo and then tested for TRAM protein expression by Western blotting.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Multiplex Assay

    SLAMF1 is enriched in the Rab11-positive ERCs in unstimulated macrophages, and SLAMF1 expression is induced by LPS and several other TLR ligands in primary human monocytes and macrophages. (A) Monocytes, macrophages, and differentiated THP-1 cells stained with antibodies against SLAMF1 (green) and GM130 (red) and imaged by confocal microscopy. (B) 3D model of cis-Golgi (GM130) and SLAMF1 in THP-1 cells. Z stacks from the GM130 and SLAMF1 channels were obtained using high-resolution confocal microscopy followed by 3D modeling in IMARIS software. (C) Macrophages stained for SLAMF1 and Rab11 (ERC marker). Representative image. Overlapping pixels for SLAMF1 and Rab11 are shown in the white overlap. tM1 = 0.683 ± 0.08 (mean with SD) for z stacks of ERCs as ROIs (30 ROIs analyzed per donor) where tM1 was the Manders’s colocalization coefficient with thresholds calculated in the Coloc 2 Fiji plugin with anti-SLAMF1 staining as first channel. (D) Macrophages costained for SLAMF1 and EEA1. (E) Macrophages costained for SLAMF1 and LAMP1. Colocalization accessed for z stacks for at least 30 cells for each experiment (four total) showing no colocalization for markers in both D and E. (F) Flow cytometry analysis of SLAMF1 surface expression by primary macrophages and differentiated THP-1 cells. Cells were costained for SLAMF1 and CD14 and gated for CD14-positive cells (primary cells) or stained for SLAMF1 (THP-1 cells). (G) Flow cytometry analysis of SLAMF1 surface expression by human macrophages stimulated by ultrapure K12 LPS (100 ng/ml) for 2, 4, and 6 h. (H) Western blot analysis of lysates from primary human macrophages stimulated by LPS for 2, 4, and 6 h. Graphs present mean values for three biological replicates with SD. Molecular weight is given in kilodaltons. (I and J ) Quantification of SLAMF1 mRNA expression by qPCR in monocytes (I) and macrophages (J) stimulated by TLRs’ ligands FSL-1 (20 ng/ml), K12 LPS (100 ng/ml), and CL075 (1 μg/ml; both I and J) as well as R848 (1 μg/ml), Pam3Cys (P3C; 1 μg/ml), or K12 E. coli particles (20/cell; I only). Results are presented as means with SD. Statistical significance between groups was evaluated by a two-tailed t test. *, P

    Journal: The Journal of Cell Biology

    Article Title: SLAMF1 is required for TLR4-mediated TRAM-TRIF–dependent signaling in human macrophages

    doi: 10.1083/jcb.201707027

    Figure Lengend Snippet: SLAMF1 is enriched in the Rab11-positive ERCs in unstimulated macrophages, and SLAMF1 expression is induced by LPS and several other TLR ligands in primary human monocytes and macrophages. (A) Monocytes, macrophages, and differentiated THP-1 cells stained with antibodies against SLAMF1 (green) and GM130 (red) and imaged by confocal microscopy. (B) 3D model of cis-Golgi (GM130) and SLAMF1 in THP-1 cells. Z stacks from the GM130 and SLAMF1 channels were obtained using high-resolution confocal microscopy followed by 3D modeling in IMARIS software. (C) Macrophages stained for SLAMF1 and Rab11 (ERC marker). Representative image. Overlapping pixels for SLAMF1 and Rab11 are shown in the white overlap. tM1 = 0.683 ± 0.08 (mean with SD) for z stacks of ERCs as ROIs (30 ROIs analyzed per donor) where tM1 was the Manders’s colocalization coefficient with thresholds calculated in the Coloc 2 Fiji plugin with anti-SLAMF1 staining as first channel. (D) Macrophages costained for SLAMF1 and EEA1. (E) Macrophages costained for SLAMF1 and LAMP1. Colocalization accessed for z stacks for at least 30 cells for each experiment (four total) showing no colocalization for markers in both D and E. (F) Flow cytometry analysis of SLAMF1 surface expression by primary macrophages and differentiated THP-1 cells. Cells were costained for SLAMF1 and CD14 and gated for CD14-positive cells (primary cells) or stained for SLAMF1 (THP-1 cells). (G) Flow cytometry analysis of SLAMF1 surface expression by human macrophages stimulated by ultrapure K12 LPS (100 ng/ml) for 2, 4, and 6 h. (H) Western blot analysis of lysates from primary human macrophages stimulated by LPS for 2, 4, and 6 h. Graphs present mean values for three biological replicates with SD. Molecular weight is given in kilodaltons. (I and J ) Quantification of SLAMF1 mRNA expression by qPCR in monocytes (I) and macrophages (J) stimulated by TLRs’ ligands FSL-1 (20 ng/ml), K12 LPS (100 ng/ml), and CL075 (1 μg/ml; both I and J) as well as R848 (1 μg/ml), Pam3Cys (P3C; 1 μg/ml), or K12 E. coli particles (20/cell; I only). Results are presented as means with SD. Statistical significance between groups was evaluated by a two-tailed t test. *, P

    Article Snippet: Transduced THP-1 cells were selected with puromycin (1 μg/ml) for 1 mo and then tested for TRAM protein expression by Western blotting.

    Techniques: Expressing, Staining, Confocal Microscopy, Software, Marker, Flow Cytometry, Cytometry, Western Blot, Molecular Weight, Real-time Polymerase Chain Reaction, Two Tailed Test

    Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the pLVX-IRES-GFP vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.

    Journal: PLoS ONE

    Article Title: Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer

    doi: 10.1371/journal.pone.0198347

    Figure Lengend Snippet: Generation and characterization of FOLR1-CAR T cells. T cells were obtained from human PBMCs of three healthy donors and the FOLR1-CAR gene was cloned into the pLVX-IRES-GFP vector and the lentivirus infection efficiency was determined. (A) The growth rate of infected or untransduced T cells was measured by CellTiter-Glo assay for 12 days. (B) Lentivirus infection rate was analyzed by GFP fluorescence on day 12 using FACS analysis. (C) Expression of FOLR1-CAR in T cells was measured by western blot analysis. (D-E) The population of T cells was evaluated by FACS analysis using CD3, CD4, and CD8 antibodies on day 12. Experiments were repeated three times with similar results. The data are represented as the mean of luminescence ± SD and positive rate ± SD (%) from triplicate cultures. Statistical analysis was performed using the two-sided unpaired t-test.

    Article Snippet: Lentiviral expression vectors, including pLVX-Puro (Cat. #632164) and pLVX-IRES-ZsGreen1 (Cat. #632187), were obtained from Clontech (USA).

    Techniques: Clone Assay, Plasmid Preparation, Infection, Glo Assay, Fluorescence, FACS, Expressing, Western Blot

    Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry. GFP, green fluorescent protein; RFP, red fluorescent protein; HIV, human immunodeficiency virus; SFFV, spleen focus-forming virus.

    Journal: Molecular Medicine Reports

    Article Title: Selection of an optimal promoter for gene transfer in normal B cells

    doi: 10.3892/mmr.2017.6974

    Figure Lengend Snippet: Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry. GFP, green fluorescent protein; RFP, red fluorescent protein; HIV, human immunodeficiency virus; SFFV, spleen focus-forming virus.

    Article Snippet: The following lentiviral vectors were used in this study: pLVX–IRES-Puro and pLVX-IRES-ZsGreen1 (both from Clontech, Takara Bio Europe), pGIPZ (Open Biosystems; GE Healthcare Life Sciences), pLVTHM (AddGene, Inc., Cambridge, MA, USA).

    Techniques: Transduction, Modification, Flow Cytometry, Cytometry

    Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry.

    Journal: Molecular Medicine Reports

    Article Title: Selection of an optimal promoter for gene transfer in normal B cells

    doi: 10.3892/mmr.2017.6974

    Figure Lengend Snippet: Transduction of Raji cells with plasmids containing various promoters. Raji lymphoma cells were modified with pLVX ZsGreen1, pLVTHM, pGIPZ and HIV-SFFV-RFP. The percentage of fluorescent (GFP- or RFP-positive) cells was determined using flow cytometry.

    Article Snippet: The following lentiviral vectors were used in this study: pLVX–IRES-Puro and pLVX-IRES-ZsGreen1 (both from Clontech, Takara Bio Europe), pGIPZ (Open Biosystems; GE Healthcare Life Sciences), pLVTHM (AddGene, Inc., Cambridge, MA, USA).

    Techniques: Transduction, Modification, Flow Cytometry, Cytometry