Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: Quercetin acutely relaxes airway smooth muscle and potentiates β-agonist-induced relaxation via dual phosphodiesterase inhibition of PLCβ and PDE4

doi: 10.1152/ajplung.00125.2013

Figure Lengend Snippet: Quercetin attenuates purified phosphatidylinositol-specific phospholipase C (PLCβ) activity. Using purified phosphatidylinositol-specific PLC, enzyme activity was measured using a substrate that fluoresces when cleaved. Quercetin inhibited enzyme

Article Snippet: Purified phosphatidylinositol-specific phospholipase C (PLCβ) was obtained from Life Technologies (P6466).

Techniques: Purification, Activity Assay, Planar Chromatography

Journal: Diabetes

Article Title: Tub Has a Key Role in Insulin and Leptin Signaling and Action In Vivo in Hypothalamic Nuclei

doi: 10.2337/db11-1388

Figure Lengend Snippet: Tub nuclear translocation in response to hormones in hypothalamic nuclei. A pool of 20 mice was used for each time point ( A – D ). Arc, MH, PVN, and LH were dissected from the same mice, and the plasma membrane and nucleus fractions were obtained from the same samples. E – I : Whole hypothalami of 14 mice were used. Plasma membranes and nuclear lysates underwent IP and were blotted (IB) with anti-Tub antibody. Tub nuclear translocation in response to ICV (2 μg) insulin (0, 15, 30, 90 min) or (10 ng) leptin (0, 7, 20 min) was investigated in Arc ( A ), MH ( B ), PVN ( C ), and LH ( D ) of mice on chow. Tub nuclear translocation in response to ICV ( E ) 100 μmol/L acetylcholine (Ach) (0, 30, 120 min), ( F ) 1 μmol/L specific PLCβ inhibitor (U73122) plus Ach (0, 10, 30, 120 min), ( G ) 1 μmol/L unspecific PLCβ inhibitor (U73343) plus Ach (0, 10, 30, 120 min), U73122 plus insulin (0, 30, 120 min) ( H ), and U73122 plus leptin (0, 120 min) ( I ) were investigated in the hypothalamus of mice fed chow diet. U73122 and U73343 inhibitors were administered 30 min before hormone injections. ICV, ICV injection.

Article Snippet: The specific phospholipase Cβ (PLCβ) inhibitor U73122 (1 μmol/L; Sigma-Aldrich) was previously injected to block Tub nuclear translocation induced by the hormones.

Techniques: Translocation Assay, Mouse Assay, Injection

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: WenTong HuoXue Cream Can Inhibit the Reduction of the Pain-Related Molecule PLC-β3 in the Dorsal Root Ganglion of a Rat Model of Diabetic Peripheral Neuropathy

doi: 10.1155/2018/3416936

Figure Lengend Snippet: WTHX-Cream can increase PLC- β expression in the DRG of rats. (a) PLC- β 3 mRNA; ((b) and (c)) PLC- β 3 protein. All values given are the mean ± SD. ∗ P

Article Snippet: Semidry electrophoresis gels were transferred to PVDF membranes and then placed in confining liquid containing 5% skim milk powder for 1 h. Thereafter, a rabbit anti-PLC-β 3 antibody (dilution ratio 1 : 1000, purchased from Abnova, batch number PAB17281) was added, gently stirred, and incubated at 4°C overnight.

Techniques: Planar Chromatography, Expressing

Journal: Frontiers in Physiology

Article Title: Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication

doi: 10.3389/fphys.2013.00058

Figure Lengend Snippet: Pattern of GFP fluorescence in transgenic PLCβ2 promoter-GFP mice correlates with endogenous PLCβ2 expression in cells of the corpus mucosa adjacent to the fundus compartment. (A–C) Analysis of cross sections through the fundus/corpus transition zone of PLCβ 2 promoter-GFP transgenic mice. PLCβ 2-immunostaining (red) (A) reveals a cluster of elongated cells located in the most apical layer of the corpus mucosa (denoted by the white dotted line ) adjoining the fundus epithelium. Intrinsically green fluorescent cells (B) are located in the same regions within the mucosa and display the morphology of cells visualized by the PLCβ 2 antibody. Overlay (C) of (A) and (B) clearly demonstrates the coexpression of PLCβ2 and GFP in a subset of cells. The cells exhibit very long apical processes extending into the lumen of the “gastric groove.” Sections were counterstained with DAPI ( blue ). Scale bars: (A–C) = 20 μm.

Article Snippet: In the taste sensory cells activation of the G-protein coupled receptor elicits a second messenger cascade mediated by the characteristic phospholipase C-subtype, PLCβ 2 (Rössler et al., ; Margolskee, ; Montmayeur and Matsunami, ; Medler and Kinnamon, ).

Techniques: Fluorescence, Transgenic Assay, Mouse Assay, Expressing, Immunostaining

Journal: Frontiers in Physiology

Article Title: Band-like arrangement of taste-like sensory cells at the gastric groove: evidence for paracrine communication

doi: 10.3389/fphys.2013.00058

Figure Lengend Snippet: PLCβ2-GFP-expressing cells of the corpus mucosa underneath the “limiting ridge” are immunoreactive for gustducin and TRPM5. (A–C) Distribution pattern of gustducin (red ) was assessed on cross sections through the corpus mucosa adjoining the fundus tissue of PLCβ 2 promoter-GFP transgenic mice. The white dotted line borders the most apical cell layer from the residual corpus mucosa. Cluster of cells immunostained for gustducin (B) underneath the “limiting ridge” exhibit an elongated cylindrical morphology and strongly labeled apical microvilli tufts. Pattern of intrinsic GFP fluorescence (A) is reminiscent of the labeling pattern obtained with the gustducin antibody (B) . Overlay (C) of (A) and (B) shows that a subpopulation of cells express both gustducin and GFP. (D–F) Consecutive sections to that shown in (A–C) stained with the TRPM5 antibody ( red ). TRPM5-immunoreactivity reveals a cluster of cells (E) resembling that of PLCβ2-GFP-expressing cells (D) , in both morphology and position, respectively. Overlay (F) of (D) and (E) indicates a complete overlap of GFP and TRPM5 in a subpopulation of corpus mucosal cells. Sections were counterstained with DAPI ( blue ). Scale bars : ( A–F ) = 20 μm.

Article Snippet: In the taste sensory cells activation of the G-protein coupled receptor elicits a second messenger cascade mediated by the characteristic phospholipase C-subtype, PLCβ 2 (Rössler et al., ; Margolskee, ; Montmayeur and Matsunami, ; Medler and Kinnamon, ).

Techniques: Expressing, Transgenic Assay, Mouse Assay, Labeling, Fluorescence, Staining