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    Thermo Fisher platinum high fidelity taq polymerase
    <t>Smad4</t> mutagenesis. (A) Schematic of mutations introduced into wild-type HA-tagged murine Smad4; DBD, DNA-binding domain; NES, nuclear export signal. Briefly, WT HA-Smad4 was used as a template for PCR reactions using Platinum High Fidelity <t>Taq</t> polymerase
    Platinum High Fidelity Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum high fidelity taq polymerase/product/Thermo Fisher
    Average 99 stars, based on 1654 article reviews
    Price from $9.99 to $1999.99
    platinum high fidelity taq polymerase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher platinum taq dna polymerase
    <t>Smad4</t> mutagenesis. (A) Schematic of mutations introduced into wild-type HA-tagged murine Smad4; DBD, DNA-binding domain; NES, nuclear export signal. Briefly, WT HA-Smad4 was used as a template for PCR reactions using Platinum High Fidelity <t>Taq</t> polymerase
    Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 25289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq dna polymerase/product/Thermo Fisher
    Average 92 stars, based on 25289 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    91
    Thermo Fisher platinum taq dna polymerase high fidelity kit
    <t>Smad4</t> mutagenesis. (A) Schematic of mutations introduced into wild-type HA-tagged murine Smad4; DBD, DNA-binding domain; NES, nuclear export signal. Briefly, WT HA-Smad4 was used as a template for PCR reactions using Platinum High Fidelity <t>Taq</t> polymerase
    Platinum Taq Dna Polymerase High Fidelity Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq dna polymerase high fidelity kit/product/Thermo Fisher
    Average 91 stars, based on 521 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase high fidelity kit - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Smad4 mutagenesis. (A) Schematic of mutations introduced into wild-type HA-tagged murine Smad4; DBD, DNA-binding domain; NES, nuclear export signal. Briefly, WT HA-Smad4 was used as a template for PCR reactions using Platinum High Fidelity Taq polymerase

    Journal: Journal of Cell Science

    Article Title: Postnatal ablation of osteoblast Smad4 enhances proliferative responses to canonical Wnt signaling through interactions with β-catenin

    doi: 10.1242/jcs.132233

    Figure Lengend Snippet: Smad4 mutagenesis. (A) Schematic of mutations introduced into wild-type HA-tagged murine Smad4; DBD, DNA-binding domain; NES, nuclear export signal. Briefly, WT HA-Smad4 was used as a template for PCR reactions using Platinum High Fidelity Taq polymerase

    Article Snippet: Briefly, WT HA-Smad4 was used as a template for PCR reaction using Platinum High Fidelity Taq polymerase (Invitrogen) and the following primers: (ΔDBD F) 5′-phospho-CATGTGATCTATGCCCGTC-3′ and (ΔDBD R) 5′-phospho-TCCATCCAATGTTCTCTGTAT-3′; (ΔNES F) 5′-phospho-AGTAATGCTCCAAGTATGTTA-3′ and (ΔNES R) 5′-phospho-GACAACCCGCTCATAGTG-3′; (ΔMH2 F) 5′-phospho-TGCTGGATTGAGATTCACCT-3′ and (ΔMH2 R) 5′-phospho-AGGATGATTGGAAATGGGAG-3′; (ΔMH1 F) 5′-phospho-TCACCTGGAATTGATCTCTC-3′ and (ΔMH1 R) 5′-phospho-GCTCAGACAGGCATCGTT-3′; (ΔLinker F) 5′-phospho-CATCCTGCTCCTGAGTAC-3′ and (ΔLinker R) 5′-phospho-CTGCAGTGTTAATCCTGA G-3′; (R100T F) 5′-phospho-ACGTGGCCTGATCTACACAAGAATG-3′ and (R100T R) 5′-phospho-CGTCCACAGACGGGCATAGATCAC-3′.

    Techniques: Mutagenesis, Binding Assay, Polymerase Chain Reaction

    RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum Taq DNA Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.

    Journal: Oncology Letters

    Article Title: Absence of the JAZF1/SUZ12 chimeric transcript in the immortalized non-neoplastic endometrial stromal cell line T HESCs

    doi: 10.3892/ol.2010.185

    Figure Lengend Snippet: RT-PCR analysis of the T HESCs cell line. (A) For the detection of the JAZF1/SUZ12 chimeric transcript, 1 μl cDNA was used as a template in PCR amplification with the primer combinations: human-JAZF1-284/Fusion-541R, Rhesus-JAZF1-284/Fusion-541R, human-JAZF1-286F/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R, the enzyme Platinum Taq DNA Polymerase High Fidelity and a touchdown PCR cycling protocol. Using the human-JAZF1-284/Fusion-541R and human-JAZF1-286F/Fusion-541R, JAZF1/SUZ12 chimeric transcripts were amplified in an endometrial stromal sarcoma carrying the t(7;17) chromosomal aberration [t(7;17)-ESS], whereas no JAZF1/SUZ12 chimeric transcripts were amplified in the T HESCs cell line. The Rhesus-JAZF1-284/Fusion-541R and Rhesus-JAZF1-286F/Fusion-541R did not generate any PCR products. (B) RT-PCR analysis showed that in the T HESCs cell line, both the normal JAZF1 and SUZ12 genes are expressed. The primer set for JAZF1 amplified a cDNA fragment that corresponds to the entire open reading of JAZF1 . The amplified cDNA fragment of SUZ12 contained part of exon 1, exons 2–6 and part of exon 7. M, 100-bp DNA ladder. Blank, no RNA in the cDNA synthesis.

    Article Snippet: For the detection of the JAZF1/SUZ12 fusion transcript, PCR was performed with Platinum Taq DNA Polymerase High Fidelity (Invitrogen).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Touchdown PCR

    Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid DNA or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding Taq Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P

    Journal: Genes to cells : devoted to molecular & cellular mechanisms

    Article Title: Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma

    doi: 10.1111/j.1365-2443.2010.01483.x

    Figure Lengend Snippet: Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid DNA or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding Taq Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P

    Article Snippet: PCR was carried out in a 50-μL reaction mixture containing 5 μL 10× high-fidelity PCR buffer, 1 μL dNTP (10 μM each), 2 μL MgSO4 (50 μM), 4 μL primers (5 μM each), 1 μL template cDNA and 1 μL Platinum® Taq High Fidelity DNA Polymerase (5 U) (Invitrogen).

    Techniques: Recombinant, Plasmid Preparation, Transfection