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  • 99
    Thermo Fisher platinum taq polymerase
    Gene regulation by fatty acids. Myotubes were incubated with fatty acids (100 µM) or BSA (40 µM, control) for 24 h and harvested for RNA isolation. Real-time <t>PCR</t> was performed with platinum <t>Taq</t> polymerase and SYBR green on an iCycler PCR
    Platinum Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9035 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq polymerase/product/Thermo Fisher
    Average 99 stars, based on 9035 article reviews
    Price from $9.99 to $1999.99
    platinum taq polymerase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taq dna polymerase platinum
    Gene regulation by fatty acids. Myotubes were incubated with fatty acids (100 µM) or BSA (40 µM, control) for 24 h and harvested for RNA isolation. Real-time <t>PCR</t> was performed with platinum <t>Taq</t> polymerase and SYBR green on an iCycler PCR
    Taq Dna Polymerase Platinum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase platinum/product/Thermo Fisher
    Average 99 stars, based on 7307 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase platinum - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    Gene regulation by fatty acids. Myotubes were incubated with fatty acids (100 µM) or BSA (40 µM, control) for 24 h and harvested for RNA isolation. Real-time PCR was performed with platinum Taq polymerase and SYBR green on an iCycler PCR

    Journal: Journal of Lipid Research

    Article Title: Metabolic switching of human myotubes is improved by n-3 fatty acids

    doi: 10.1194/jlr.M003319

    Figure Lengend Snippet: Gene regulation by fatty acids. Myotubes were incubated with fatty acids (100 µM) or BSA (40 µM, control) for 24 h and harvested for RNA isolation. Real-time PCR was performed with platinum Taq polymerase and SYBR green on an iCycler PCR

    Article Snippet: Real-time PCR was performed with platinum Taq polymerase (Invitrogen, Breda, The Netherlands) and SYBR green on an iCycler PCR machine (Bio-Rad Laboratories).

    Techniques: Incubation, Isolation, Real-time Polymerase Chain Reaction, SYBR Green Assay, Polymerase Chain Reaction

    Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.

    Journal: PLoS ONE

    Article Title: Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria

    doi: 10.1371/journal.pone.0007010

    Figure Lengend Snippet: Detection of the beta-lactamase gene in commercial Taq polymerase. Four dilutions of Amplitaq DNA polymerase were tested with primers for the beta-lactamase gene in the presence of 10 3 pUC19 plasmids (labeled 1000 pUC19 genomes) or H 2 O.

    Article Snippet: Taq polymerases The following DNA polymerases from commercial vendors designed for qPCR were used in the experiments reported here: Amplitaq Gold (ABI, CA; Roche lot # J02913); Platinum Taq (Invitrogen, CA; cat # 10966–026; lot 1169610); Platinum HiFi Taq (Invitrogen, CA; cat# 11304–011; lot# 1267490); HotStar Taq (Qiagen, CA; Mat # 1007837; lot # 124125007); JumpStart Taq (Sigma, MO; cat # D-6558; lot # 71K9029).

    Techniques: Labeling

    Reportable range for T. cruzi and triatomine intestine unit quantification by real-time qPCR. Multiplex Taq Man qPCR assays were carried out with serially diluted DNA extracted from reconstituted triatomine intestine samples containing T. cruzi epimastigotes, ranging from 10 5 to 0.5 T. cruzi equivalents ( a ) and 5 to 0.002 triatomine intestine unit equivalents ( b ). The slope, R 2 and amplification efficiency (Eff) are indicated in the chart

    Journal: Parasites & Vectors

    Article Title: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

    doi: 10.1186/s13071-017-2343-x

    Figure Lengend Snippet: Reportable range for T. cruzi and triatomine intestine unit quantification by real-time qPCR. Multiplex Taq Man qPCR assays were carried out with serially diluted DNA extracted from reconstituted triatomine intestine samples containing T. cruzi epimastigotes, ranging from 10 5 to 0.5 T. cruzi equivalents ( a ) and 5 to 0.002 triatomine intestine unit equivalents ( b ). The slope, R 2 and amplification efficiency (Eff) are indicated in the chart

    Article Snippet: Multiplex conventional PCR (cPCR) Conventional PCR assays were carried out in a final volume of 50 μl, containing: 5 μl DNA (20–25 ng), 5 μl 10× Taq Platinum buffer, 0.2 mM dNTPs, 4.5 mM MgCl2 , 1.25 U Taq Platinum DNA polymerase (Life Technologies, Carlsbad, CA, USA), 200 nM 121/122 primers (T. cruzi kDNA) [ , ] and 100 nM P2B/P6R primers (triatomine 12S rRNA gene).

    Techniques: Real-time Polymerase Chain Reaction, Multiplex Assay, Amplification