platinium taq dna polymerase Thermo Fisher Search Results


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  • 90
    Thermo Fisher taq dna polymerase
    Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 36230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq dna polymerase
    Confirmation of spy0129 Deletion Mutant and Adherence Assay Top panel is a schematic representation of the spy0127–0130 cluster. Position of primers for RT-PCR are indicated by arrows. (A) Results of RT-PCR analysis on mRNA from two putative deletion mutants (Δ1 and Δ2) and the parental SF370 strain (P). mRNA was isolated from mid log-phase or stationary phase cells (indicated below panel) and reverse-transcribed with two primer combinations, which are indicated at the top of the lanes as primer set A (0129F–0129R) and primer set B (0129F–0130R). cDNA products were separated on a 1% agarose gel and visualized by ethidium bromide staining. The expected sizes of resulting cDNAs from SF370 using primer set A is 365 bp and using primer set B is 1200 bp. Control reactions (C) containing mid-log phase mRNA and <t>Taq</t> <t>DNA</t> polymerase instead of reverse transcriptase are indicated. Lane 1 contains 1 kb Plus DNA ladder (1 μg; Invitrogen). (B) Results of the pharyngeal cell adherence assay (detailed in Methods), comparing parental strain SF370 to the spy0129–0130 isogenic mutant, SF370Δ spy0129 .2 (abbreviated as Δ spy0129 ). Adherent streptococci are reported as the percentage of total number of streptococci added as inoculum to pharyngeal cell monolayers. Statistical significance (reported as p -value) was determined by Student's t- test.
    Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 20820 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platnium taq dna polymerase
    Confirmation of spy0129 Deletion Mutant and Adherence Assay Top panel is a schematic representation of the spy0127–0130 cluster. Position of primers for RT-PCR are indicated by arrows. (A) Results of RT-PCR analysis on mRNA from two putative deletion mutants (Δ1 and Δ2) and the parental SF370 strain (P). mRNA was isolated from mid log-phase or stationary phase cells (indicated below panel) and reverse-transcribed with two primer combinations, which are indicated at the top of the lanes as primer set A (0129F–0129R) and primer set B (0129F–0130R). cDNA products were separated on a 1% agarose gel and visualized by ethidium bromide staining. The expected sizes of resulting cDNAs from SF370 using primer set A is 365 bp and using primer set B is 1200 bp. Control reactions (C) containing mid-log phase mRNA and <t>Taq</t> <t>DNA</t> polymerase instead of reverse transcriptase are indicated. Lane 1 contains 1 kb Plus DNA ladder (1 μg; Invitrogen). (B) Results of the pharyngeal cell adherence assay (detailed in Methods), comparing parental strain SF370 to the spy0129–0130 isogenic mutant, SF370Δ spy0129 .2 (abbreviated as Δ spy0129 ). Adherent streptococci are reported as the percentage of total number of streptococci added as inoculum to pharyngeal cell monolayers. Statistical significance (reported as p -value) was determined by Student's t- test.
    Platnium Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq high fidelity dna polymerase
    Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid <t>DNA</t> or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding <t>Taq</t> Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P
    Platinum Taq High Fidelity Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific platinum taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platinum Taq Dna Polymerase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 95/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hifi platinum taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Hifi Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq dna polymerase kit
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platinum Taq Dna Polymerase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum ii taq hot start dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platinum Ii Taq Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum pcr taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platinum Pcr Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher recombinant platinum taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Recombinant Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hot start dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr buffer
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq hot start polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platinum Taq Hot Start Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher antibody complexed platinum taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Antibody Complexed Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher master mix platinum taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Master Mix Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum superfi dna taq polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platinum Superfi Dna Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pwo platinum taq dna polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Pwo Platinum Taq Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platininum taq polymerase
    Amplification of Listeria monocytogenes EGDe and Δ prfA <t>DNA</t> using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum <t>Taq</t> polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values
    Platininum Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum genotype tsp taq polymerase
    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
    Platinum Genotype Tsp Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq dna polymerase high fidelity pcr kit
    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
    Platinum Taq Dna Polymerase High Fidelity Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinium taq polymerase kit
    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
    Platinium Taq Polymerase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pet24a n1 platinum taq hifi dna polymerase
    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
    Pet24a N1 Platinum Taq Hifi Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq high fidelity dna polymerase system
    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of <t>PCR</t> products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with <t>Taq</t> I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).
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    Image Search Results


    Confirmation of spy0129 Deletion Mutant and Adherence Assay Top panel is a schematic representation of the spy0127–0130 cluster. Position of primers for RT-PCR are indicated by arrows. (A) Results of RT-PCR analysis on mRNA from two putative deletion mutants (Δ1 and Δ2) and the parental SF370 strain (P). mRNA was isolated from mid log-phase or stationary phase cells (indicated below panel) and reverse-transcribed with two primer combinations, which are indicated at the top of the lanes as primer set A (0129F–0129R) and primer set B (0129F–0130R). cDNA products were separated on a 1% agarose gel and visualized by ethidium bromide staining. The expected sizes of resulting cDNAs from SF370 using primer set A is 365 bp and using primer set B is 1200 bp. Control reactions (C) containing mid-log phase mRNA and Taq DNA polymerase instead of reverse transcriptase are indicated. Lane 1 contains 1 kb Plus DNA ladder (1 μg; Invitrogen). (B) Results of the pharyngeal cell adherence assay (detailed in Methods), comparing parental strain SF370 to the spy0129–0130 isogenic mutant, SF370Δ spy0129 .2 (abbreviated as Δ spy0129 ). Adherent streptococci are reported as the percentage of total number of streptococci added as inoculum to pharyngeal cell monolayers. Statistical significance (reported as p -value) was determined by Student's t- test.

    Journal: PLoS Computational Biology

    Article Title: Novel Algorithms Reveal Streptococcal Transcriptomes and Clues about Undefined Genes

    doi: 10.1371/journal.pcbi.0030132

    Figure Lengend Snippet: Confirmation of spy0129 Deletion Mutant and Adherence Assay Top panel is a schematic representation of the spy0127–0130 cluster. Position of primers for RT-PCR are indicated by arrows. (A) Results of RT-PCR analysis on mRNA from two putative deletion mutants (Δ1 and Δ2) and the parental SF370 strain (P). mRNA was isolated from mid log-phase or stationary phase cells (indicated below panel) and reverse-transcribed with two primer combinations, which are indicated at the top of the lanes as primer set A (0129F–0129R) and primer set B (0129F–0130R). cDNA products were separated on a 1% agarose gel and visualized by ethidium bromide staining. The expected sizes of resulting cDNAs from SF370 using primer set A is 365 bp and using primer set B is 1200 bp. Control reactions (C) containing mid-log phase mRNA and Taq DNA polymerase instead of reverse transcriptase are indicated. Lane 1 contains 1 kb Plus DNA ladder (1 μg; Invitrogen). (B) Results of the pharyngeal cell adherence assay (detailed in Methods), comparing parental strain SF370 to the spy0129–0130 isogenic mutant, SF370Δ spy0129 .2 (abbreviated as Δ spy0129 ). Adherent streptococci are reported as the percentage of total number of streptococci added as inoculum to pharyngeal cell monolayers. Statistical significance (reported as p -value) was determined by Student's t- test.

    Article Snippet: RT–PCR generation of amplicons was performed with the SuperScript III One-Step RT–PCR system with Platinum Taq DNA polymerase (Invitrogen) in reaction mixtures (50 μl) containing 0.2 μM of each gene-specific forward and reverse primers and 0.1 μg of DNase-treated, purified total RNA from late-log phase cultures (OD = 0.7) of strain SF370.

    Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Isolation, Agarose Gel Electrophoresis, Staining

    Confirmation of speH Deletion Mutant and Pharyngeal Cell Adherence Assay (A) Results of RT-PCR and PCR analyses of total RNA preparations isolated from mid-log (OD = 0.4) and stationary phase (OD = 1) cultures of the ΔspeH deletion mutant (ΔL and ΔS, respectively), and stationary phase cultures of the SF370 parental strain (P). RNA was reverse-transcribed as described in Methods. To assess genomic DNA contamination, control reactions containing Taq DNA polymerase instead of reverse transcriptase were included. cDNA products were separated on a 1% agarose gel and visualized by ethidium bromide staining. Lanes containing products from either the RT-PCR or PCR analysis are designated at the bottom of the panel. Lanes labeled MW contain 1 kb Plus DNA ladder (1 μg; Invitrogen). (B) Results of the pharyngeal cell adherence assay (detailed in Methods), comparing parental strain SF370 with the deletion mutant SF370Δ speH (abbreviated Δ speH ). Adherent streptococci are reported as the percentage of total number of streptococci added as inoculum to pharyngeal cell monolayers. Statistical significance (reported as p value) was determined by Student's t- test.

    Journal: PLoS Computational Biology

    Article Title: Novel Algorithms Reveal Streptococcal Transcriptomes and Clues about Undefined Genes

    doi: 10.1371/journal.pcbi.0030132

    Figure Lengend Snippet: Confirmation of speH Deletion Mutant and Pharyngeal Cell Adherence Assay (A) Results of RT-PCR and PCR analyses of total RNA preparations isolated from mid-log (OD = 0.4) and stationary phase (OD = 1) cultures of the ΔspeH deletion mutant (ΔL and ΔS, respectively), and stationary phase cultures of the SF370 parental strain (P). RNA was reverse-transcribed as described in Methods. To assess genomic DNA contamination, control reactions containing Taq DNA polymerase instead of reverse transcriptase were included. cDNA products were separated on a 1% agarose gel and visualized by ethidium bromide staining. Lanes containing products from either the RT-PCR or PCR analysis are designated at the bottom of the panel. Lanes labeled MW contain 1 kb Plus DNA ladder (1 μg; Invitrogen). (B) Results of the pharyngeal cell adherence assay (detailed in Methods), comparing parental strain SF370 with the deletion mutant SF370Δ speH (abbreviated Δ speH ). Adherent streptococci are reported as the percentage of total number of streptococci added as inoculum to pharyngeal cell monolayers. Statistical significance (reported as p value) was determined by Student's t- test.

    Article Snippet: RT–PCR generation of amplicons was performed with the SuperScript III One-Step RT–PCR system with Platinum Taq DNA polymerase (Invitrogen) in reaction mixtures (50 μl) containing 0.2 μM of each gene-specific forward and reverse primers and 0.1 μg of DNase-treated, purified total RNA from late-log phase cultures (OD = 0.7) of strain SF370.

    Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Isolation, Agarose Gel Electrophoresis, Staining, Labeling

    Selector construct and circularization reaction principles. ( A ) The selector consists of two oligonucleotides, one selector probe of ∼70 nt and one general vector oligonucleotide of 34 nt. The selector probe has two target-specific ends (black) of ∼18 nt complementary to the selected target and a primer-pair motif (gray) of 34 nt. The vector oligonucleotide is complementary to the primer-pair motif (gray). ( B ) The circularization procedure starts with digestion of the DNA to generate targets with defined ends. The digested DNA is then combined with selectors and the mixture is denaturated, allowing the selectors to hybridize to their respective targets. This can be performed in two different ways: (i) a selector probe hybridizes to both ends of the selected target and the ends are connected to the vector by ligation, or (ii) a selector probe hybridizes with one end to the 3′ end of the selected target and the other end to an internal sequence of the target, forming a branched structure that can be cleaved by the endonucleolytic activity of Taq DNA polymerase. Both ends of the selected single-stranded target are now ready for ligation to the vector oligonucleotide, forming a closed circular molecule. The circularization mixture is then exonuclease-treated and finally PCR-amplified using a common primer pair.

    Journal: Nucleic Acids Research

    Article Title: Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments

    doi: 10.1093/nar/gni070

    Figure Lengend Snippet: Selector construct and circularization reaction principles. ( A ) The selector consists of two oligonucleotides, one selector probe of ∼70 nt and one general vector oligonucleotide of 34 nt. The selector probe has two target-specific ends (black) of ∼18 nt complementary to the selected target and a primer-pair motif (gray) of 34 nt. The vector oligonucleotide is complementary to the primer-pair motif (gray). ( B ) The circularization procedure starts with digestion of the DNA to generate targets with defined ends. The digested DNA is then combined with selectors and the mixture is denaturated, allowing the selectors to hybridize to their respective targets. This can be performed in two different ways: (i) a selector probe hybridizes to both ends of the selected target and the ends are connected to the vector by ligation, or (ii) a selector probe hybridizes with one end to the 3′ end of the selected target and the other end to an internal sequence of the target, forming a branched structure that can be cleaved by the endonucleolytic activity of Taq DNA polymerase. Both ends of the selected single-stranded target are now ready for ligation to the vector oligonucleotide, forming a closed circular molecule. The circularization mixture is then exonuclease-treated and finally PCR-amplified using a common primer pair.

    Article Snippet: To enrich for circularized DNA by degrading linear strands including selectors, 10 μl of the circularization mixtures (0.4 μg DNA) were then added to a 10 μl mixture of 5 U Exonuclease I (New England Biolabs), 110 mM Tris–HCl, pH 9.0, 3 mM MgCl2 and 0.2 μg BSA and incubated for 2 h at 37°C, followed by 95°C for 10 min. Amplification was performed using 4 μl of each exonuclease-treated circularization reaction (80 ng DNA each) added to 17 μl mixture of 1× PCR buffer (Invitrogen), supplemented with 0.5 U Platinum Taq DNA polymerase (Invitrogen), 0.25 mM dNTP, 0.4 μM Cy-3-labeled forward and reverse primer, respectively, and 2 mM MgCl2 .

    Techniques: Construct, Plasmid Preparation, Ligation, Sequencing, Activity Assay, Polymerase Chain Reaction, Amplification

    Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid DNA or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding Taq Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P

    Journal: Genes to cells : devoted to molecular & cellular mechanisms

    Article Title: Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma

    doi: 10.1111/j.1365-2443.2010.01483.x

    Figure Lengend Snippet: Analysis of the recombinant ANGPTL7/CDT6 plasmid (pNC1) in primary human trabecular meshwork cells. Two primary HTM cell lines (HTM-55 and HTM-69) were nucleofector-transfected with either ANGPTL7/CDT6 plasmid DNA or mock-transfected, and assayed at 72-h post-transfection. (A) Normalized ANGPTL7/CDT6 cDNA in the treated cells versus the mock-transfected cells. Top panel : representative C T logarithmic curve of the hybridizations of ANGPTL7/CDT6 and endogenous 18S cDNAs from treated and mock-transfected cells with their corresponding Taq Man probes. Bottom panel : fold change of ANGPTL7/CDT6 cDNA in treated versus mock-transfected, normalized to 18S and expressed as fold change mean ± range ( n = 3 * P

    Article Snippet: PCR was carried out in a 50-μL reaction mixture containing 5 μL 10× high-fidelity PCR buffer, 1 μL dNTP (10 μM each), 2 μL MgSO4 (50 μM), 4 μL primers (5 μM each), 1 μL template cDNA and 1 μL Platinum® Taq High Fidelity DNA Polymerase (5 U) (Invitrogen).

    Techniques: Recombinant, Plasmid Preparation, Transfection

    Amplification of Listeria monocytogenes EGDe and Δ prfA DNA using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum Taq polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values

    Journal: Biomolecular Detection and Quantification

    Article Title: Essential role of polymerases for assay performance – Impact of polymerase replacement in a well-established assay

    doi: 10.1016/j.bdq.2018.10.002

    Figure Lengend Snippet: Amplification of Listeria monocytogenes EGDe and Δ prfA DNA using various polymerases under prfA-standard-conditions . Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under prfA-standard-conditions (only AmpliTaq Gold 10 min denaturation) with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum Taq polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values

    Article Snippet: They were compared to our routinely used Platinum® Taq DNA Polymerase (Fisher Scientific), which is complexed with an antibody for its hot start property [ ].

    Techniques: Amplification

    Amplification of Listeria monocytogenes EGDe and Δ prfA DNA using various polymerases under optimized conditions. Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under optimized conditions with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum Taq polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values

    Journal: Biomolecular Detection and Quantification

    Article Title: Essential role of polymerases for assay performance – Impact of polymerase replacement in a well-established assay

    doi: 10.1016/j.bdq.2018.10.002

    Figure Lengend Snippet: Amplification of Listeria monocytogenes EGDe and Δ prfA DNA using various polymerases under optimized conditions. Calibration curves (ranging from 1.58 × 10 1 to 1.58 × 10 6 ITMN per reaction, copies on the x-axis and Cq on y-axis) amplified under optimized conditions with different polymerases/mastermixes (grey circles) were compared with the calibration curve amplified by Platinum Taq polymerase (black squares). All duplex reactions were displayed on top of each other with the white background for the prfA assay and grey for the Δ prfA assay. Rsq values and efficiencies (in %) were indicated for each polymerase/mastermix in the respective graph with Rsq values

    Article Snippet: They were compared to our routinely used Platinum® Taq DNA Polymerase (Fisher Scientific), which is complexed with an antibody for its hot start property [ ].

    Techniques: Amplification

    Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of PCR products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with Taq I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A nonsense mutation in zebrafish gata1 causes the bloodless phenotype in vlad tepes

    doi: 10.1073/pnas.082695299

    Figure Lengend Snippet: Detection of a nonsense point mutation in gata1 . ( a ) The chromatograms show sequence of PCR products derived from a homozygous wild-type embryo (+/+) ( Upper ) and a homozygous mutant embryo (−/−) ( Lower ) for the gata1 gene nt 1013–1017. ( b ) Genotyping of vlt m651 embryos. PCR products using primers Arg-339-S and Arg-339-AS synthesized from embryo DNA of vlt m651 incrosses were digested with Taq I and electrophoresed on a 2% agarose gel. After Taq I digestion, mutant alleles appear as 246-bp and wild-type alleles as 121- and 125-bp products, which migrate together in the gel shown. Phenotypically wild-type embryos (WT) identified by presence of circulating blood and phenotypically mutant (M) embryos identified by absence of circulating blood are shown. ( c ) A schematic representation of the N-finger, C-finger, and basic domain of Gata1 and a sequence alignment of the basic domain from different species zebrafish (zf), human (h), mouse (m), and Xenopus ).

    Article Snippet: Microsatellite markers (Invitrogen) were used in PCR with Platinum Genotype Tsp Taq polymerase (Invitrogen) according to the manufacturer's specifications.

    Techniques: Mutagenesis, Sequencing, Polymerase Chain Reaction, Derivative Assay, Synthesized, Agarose Gel Electrophoresis