Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Platelet surface CD40 ligand (CD40L) expression. CD40L mean fluorescent intensity (MFI) was measured by flow cytometric analysis in unstimulated and thrombin activated platelets. Each symbol represents an individual experiment and bars represent the mean values of each group. *p

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques: Expressing, Flow Cytometry

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Induction of human intestinal microvascular endothelial cell (HIMEC) contact dependent CD40 ligand (CD40L) on platelets. Normal platelets were left untreated, or cocultured with unstimulated or interleukin 1β (IL-1β) activated HIMEC, stained with CD40L and submitted to flow cytometric analysis. The coculture supernatants were collected and the sCD40L content measured by ELISA. (A) Percentage of CD40L positive platelets. (B) Levels of sCD40L. Data represent the values of three and four individual experiments for (A) and (B), respectively. *p

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques: Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Levels of soluble CD40 ligand (sCD40L) in the plasma of inflammatory bowel disease patients according to anatomical disease extension. *p

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques:

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Release of soluble CD40 ligand (sCD40L) per platelet. Levels of sCD40L in supernatants of unstimulated and thrombin activated platelets were normalised to a fixed number of platelets (10 9 ). *p

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques:

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Relative contribution of platelets and peripheral blood T cells (PBT) to soluble CD40 ligand (sCD40L) levels. Autologous platelets and PBT from normal and inflammatory bowel disease (IBD) subjects were cultured alone for 24 hours. Data are representative of four normal controls and seven IBD patients. *p

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques: Cell Culture

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: CD40 ligand (CD40L) protein levels in normal and inflammatory bowel disease platelets. Western blot analysis of three normal, two UC, and two CD platelet extracts. The 33 and 28 kDa protein bands represent the two isoforms of CD40L. NL, normal; CD, Crohn’s disease; UC, ulcerative colitis.

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques: Western Blot

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Levels of soluble CD40 ligand (sCD40L) in plasma of inflammatory bowel disease (IBD) patients. Each symbol represents an individual patient and bars represent the mean values of each group. *p

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques:

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Induction of human intestinal microvascular endothelial cell (HIMEC) contact dependent CD40 ligand (CD40L) on platelets. Normal platelets were left untreated, or cocultured with unstimulated or interleukin 1β (IL-1β) activated HIMEC, stained with CD40L and P-selectin antibodies, and submitted to flow cytometric analysis. The histogram shows the percentage of positive platelets, and is representative of one of three separate experiments.

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques: Staining, Flow Cytometry

Journal: Gut

Article Title: Activated platelets are the source of elevated levels of soluble CD40 ligand in the circulation of inflammatory bowel disease patients

doi:

Figure Lengend Snippet: Induction of human intestinal microvascular endothelial cell (HIMEC) contact dependent CD40 ligand (CD40L) on platelets. Normal platelets were left untreated, or cocultured with unstimulated or interleukin 1β (IL-1β) activated HIMEC, stained with CD40L and P-selectin antibodies, and submitted to flow cytometric analysis. The histogram shows the percentage of positive platelets, and is representative of one of three separate experiments.

Article Snippet: Platelets were fixed in 1% paraformaldehyde and incubated with antibodies against CD40L (FITC conjugated anti-CD40L; BD, San Jose, California, USA), P-selectin (CD62P; PE-anti-P-selectin; BD), or CD42b (Santa Cruz Biotechnology, Santa Cruz, California, USA), for 30 minutes at room temperature.

Techniques: Staining, Flow Cytometry