Journal: Current Developments in Nutrition
Article Title: Alleviation of Metabolic Endotoxemia by Milk Fat Globule Membrane: Rationale, Design, and Methods of a Double-Blind, Randomized, Controlled, Crossover Dietary Intervention in Adults with Metabolic Syndrome
Figure Lengend Snippet: Study design of the planned double-blind, randomized, controlled, crossover dietary intervention. Adults with MetS will be randomly assigned to receive either MFGM-enriched milk or a comparator beverage formulated with nonfat dairy milk powder, soy phospholipids, and a blend of palm and coconut oils (75:25) for 14 d while following a prescribed, eucaloric diet in which all foods are provided. Fasting blood samples will be collected on days 0, 7, and 14 for the measure of metabolic chemistries. On the morning of day 14, participants will provide a fecal sample that was collected during the preceding 24 h for the analysis of gut microbiota composition, SCFAs, and neutrophil-derived proinflammatory proteins. In the fasted state, participants will receive a high-fat/high-carbohydrate meal challenge that also contains nondigestible sugar probes. Blood will be collected prior to (0 min) and at 30-min intervals for 3 h following the test meal for metabolic assessments. Urine will be collected for 24 h to assess region-specific gut permeability. Upon completing the first study intervention, participants will complete an ∼2-wk washout before receiving the alternative treatment and completing the second intervention in an identical manner. MetS, metabolic syndrome; MFGM, milk fat globule membrane; MPO, myeloperoxidase; TLR4, Toll-like receptor 4.
Article Snippet: Fecal protein concentrations of calprotectin (Hycult Biotech) and myeloperoxidase (MPO; Eagle Biosciences) will be measured by ELISA to assess between-treatment differences in gut-level inflammatory responses.
Techniques: Derivative Assay, Permeability