pl3 Millipore Search Results


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  • 96
    Millipore 1 2 dioleoyl sn glycero 3 phosphocholine
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    1 2 Dioleoyl Sn Glycero 3 Phosphocholine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore amicon ultra pl 3
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Amicon Ultra Pl 3, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dapi
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 87206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bsa
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 55278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore phosphate buffered saline pbs
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Phosphate Buffered Saline Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 42746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dtt
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 36382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tri reagent
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Tri Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 45305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore pfa
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Pfa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore edta
    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) <t>1,2-dioleoyl-</t> sn <t>-glycero-3-phosphocholine</t> (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore mexiletine
    The figure shows the dose-response curve (A) of increasing concentration of H 2 O 2 and <t>mexiletine</t> (10 μM-1 mM) and the cytotoxic effect of increasing concentration (10–100 μM) of mexiletine, its pyrroline derivatives and PP2 (B) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and cell viability (%) is expressed according to the following formula: cell viability (%) = [(test value - blank)/(control value-blank) × 100], where the blank value represents that of cell-free wells, the test value represents that of cells treated with one of the test compounds and the control value represents that of not-treated cells (indicated with dashed line in B ). Each data is from at least 12–38 replicates (wells) and 3–6 different culture dishes. The statistical significance between groups was evaluated by Student's t -test as follow: *significantly different with respect to control value (0.001
    Mexiletine, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore 4 formyl 3
    The figure shows the dose-response curve (A) of increasing concentration of H 2 O 2 and <t>mexiletine</t> (10 μM-1 mM) and the cytotoxic effect of increasing concentration (10–100 μM) of mexiletine, its pyrroline derivatives and PP2 (B) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and cell viability (%) is expressed according to the following formula: cell viability (%) = [(test value - blank)/(control value-blank) × 100], where the blank value represents that of cell-free wells, the test value represents that of cells treated with one of the test compounds and the control value represents that of not-treated cells (indicated with dashed line in B ). Each data is from at least 12–38 replicates (wells) and 3–6 different culture dishes. The statistical significance between groups was evaluated by Student's t -test as follow: *significantly different with respect to control value (0.001
    4 Formyl 3, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore poly l lysine
    The figure shows the dose-response curve (A) of increasing concentration of H 2 O 2 and <t>mexiletine</t> (10 μM-1 mM) and the cytotoxic effect of increasing concentration (10–100 μM) of mexiletine, its pyrroline derivatives and PP2 (B) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and cell viability (%) is expressed according to the following formula: cell viability (%) = [(test value - blank)/(control value-blank) × 100], where the blank value represents that of cell-free wells, the test value represents that of cells treated with one of the test compounds and the control value represents that of not-treated cells (indicated with dashed line in B ). Each data is from at least 12–38 replicates (wells) and 3–6 different culture dishes. The statistical significance between groups was evaluated by Student's t -test as follow: *significantly different with respect to control value (0.001
    Poly L Lysine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27394 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tris
    The figure shows the dose-response curve (A) of increasing concentration of H 2 O 2 and <t>mexiletine</t> (10 μM-1 mM) and the cytotoxic effect of increasing concentration (10–100 μM) of mexiletine, its pyrroline derivatives and PP2 (B) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and cell viability (%) is expressed according to the following formula: cell viability (%) = [(test value - blank)/(control value-blank) × 100], where the blank value represents that of cell-free wells, the test value represents that of cells treated with one of the test compounds and the control value represents that of not-treated cells (indicated with dashed line in B ). Each data is from at least 12–38 replicates (wells) and 3–6 different culture dishes. The statistical significance between groups was evaluated by Student's t -test as follow: *significantly different with respect to control value (0.001
    Tris, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 38479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore hoechst 33342
    The MN promoter-driven cameleons can be efficiently and specifically expressed in MNs after transduction of mice with the AAV vectors in vivo . Neonatal mice (P1) were injected into the temporal vein with AAV vectors encoding the mitochondrial (panels A–C) or the ER (panels D–J) cameleon. Mice were sacrificed 4 weeks after injection, and spinal cord cross-sections were labeled with the nuclear fluorescent dye <t>Hoechst</t> 33342 (panels B,E,I) and immunostained with the MN marker SMI32 (panel H), and then observed with a fluorescence stereo-microscope (panels A–F) or a confocal microscope (panels G–J). Low-resolution stereo-microscopy images show a diffuse expression of both fluorescent Ca 2+ probes (green signal, panels A,D,G), indicating that the cameleons are effectively transduced and expressed in the spinal cord in vivo . The merged reconstruction of complete z-stacks of cells collected by confocal microscopy shows that the D4ER cameleon is expressed only in SMI32-immunopositive MNs (merged image, panel J), suggesting the cell-specific expression of the probe. Scale bars = 200 µm (A–C), 100 µm (D–F), 10 µm (G–J).
    Hoechst 33342, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore poly lactic co glycolic acid plga
    Radiosensitization by ALP-(MIs)n in vitro . (A) Representative images of clonogenic survival assays of C6 cells cultured with <t>AL-PLGA,</t> ALP-(MIs)25, and ALP-(MIs)48 under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition following treatment with 0, 2, 4, 6 and 8 Gy. (B) Clonogenic survival curves of C6 cells cultured with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition following treatment with 0, 2, 4, 6 and 8 Gy. (C) Immunocytochemical analysis of γ-H2AX expressed by C6 cells. Cells were treated by incubation with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 for 4 h under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition followed by irradiation with 2 Gy using a dose rate of 0.3 Gy min -1 . Cells were stained with an anti-γ-H2AX antibody (red) and <t>DAPI</t> (blue) 24 h after RT. Scale bar, 100 μm. (D) Quantitation of percentage of γ-H2AX positive cells. (Mean ± SD, n = 5, ** p
    Poly Lactic Co Glycolic Acid Plga, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore orlistat
    IC 50 values ( n = 3 ± standard deviation) for the inhibition of pancreatic lipase (PL) activity by <t>orlistat</t> and aqueous polyphenolic extracts of vegetables. Bars with different letters have mean values that are significantly ( p
    Orlistat, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore n acetyl l cysteine
    Piperlongumine selectively kills HNC cells (A-B) Piperlongumine induces death in HNC cells but not normal cells. Cytotoxicity was assessed by MTT assay (A), trypan blue exclusion assay, and crystal violet staining (B) after exposure to 1–15 μM piperlongumine (PL) for 48–72 h. Normal human cells (N) included oral keratinocytes (HOK), oral fibroblasts (HOF), and skin keratinocytes (HEK) isolated from human oral mucosa and skin, respectively. The cytotoxic effect of PL was blocked by the antioxidant N <t>-acetyl-L-cystine</t> (NAC, 3 mM). The error bars represent s.d. from three independent experiments, each performed with triplicate samples. * denotes p
    N Acetyl L Cysteine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore doxorubicin
    Biodistribution of EGFR-targeted and non-targeted <t>doxorubicin-liposomes</t> in mice bearing i.p. SKOV-3 xenografts. Either targeted liposomal DXR or non-targeted liposomal DXR was injected i.v. at a dose of 2 mg/kg. DXR content was assayed in the indicated tissues at 24 h post-treatment ( n = 3). Data are expressed as mean of % of injected dose (ID)/g tissue, ± SD.
    Doxorubicin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8784 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 1 chloro 4 iodobutane
    Biodistribution of EGFR-targeted and non-targeted <t>doxorubicin-liposomes</t> in mice bearing i.p. SKOV-3 xenografts. Either targeted liposomal DXR or non-targeted liposomal DXR was injected i.v. at a dose of 2 mg/kg. DXR content was assayed in the indicated tissues at 24 h post-treatment ( n = 3). Data are expressed as mean of % of injected dose (ID)/g tissue, ± SD.
    1 Chloro 4 Iodobutane, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 1 chloro 5 iodopentane
    Biodistribution of EGFR-targeted and non-targeted <t>doxorubicin-liposomes</t> in mice bearing i.p. SKOV-3 xenografts. Either targeted liposomal DXR or non-targeted liposomal DXR was injected i.v. at a dose of 2 mg/kg. DXR content was assayed in the indicated tissues at 24 h post-treatment ( n = 3). Data are expressed as mean of % of injected dose (ID)/g tissue, ± SD.
    1 Chloro 5 Iodopentane, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).

    Journal: International Journal of Molecular Sciences

    Article Title: Recombinant Lipase from Gibberella zeae Exhibits Broad Substrate Specificity: A Comparative Study on Emulsified and Monomolecular Substrate

    doi: 10.3390/ijms18071535

    Figure Lengend Snippet: Variations with surface pressure on catalytic activity of rGZEL using different phospholipids. ( A ) 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC), ( B ) l -α-phosphatidylethanolamine (PE), ( C ) l -α-phosphatidylinositol (PI), ( D ) 1,2-diacyl- sn -glycero-3-phospho- l -serine (PS), ( E ) 3- sn -phosphatidic acid sodium salt (PA), ( F ) 1,2-dioleoyl- sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG), ( G ) cardiolipin (CL), ( H ) 1,2-distearoyimonoglactosylglyceride (MGDG). Assays were carried out at room temperature in a ‘‘zero-order’’ trough (volume, 2.5 mL; surface area, 12.56 cm 2 ). Buffer: 50 mM PB (pH 6.0). Activities were expressed as the number of moles of substrate hydrolyzed per unit time and unit reaction compartment area per milligram of enzyme in the ‘‘zero-order’’ trough (moles cm −2 min −1 mg −1 ).

    Article Snippet: Tributyrin (TC4, 99%), Tricaprylin (TC8, 99%), Triolein (TC18, 99%), 1,2-dioleoyl-sn -glycero-3-phosphocholine (DOPC, ≥97%), l -α-phosphatidylinositol (PI, ≥99%), 1,2-diacyl-sn -glycero-3-phospho-l -serine (PS, ≥97%), l -α-phosphatidylethanolamine (PE, ≥99%), cardiolipin (CL, ≥98%), 3-sn -Phosphatidic acid sodium salt (PA, ≥98%), Sphingomyelin (SM, ≥98%), 1,2-dioleoyl-sn -glycero-3-phospho-rac-(1-glycerol) sodium salt (PG, ≥98%), 1,2-distearoyimonoglactosylglyceride (MGDG, ≥98%) were all from Sigma-Aldrich Corporation (St. Louis, MO, USA).

    Techniques: Activity Assay

    Optical response and some schemes of the bilayers measured under heating cycles for ( a ) 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) (ellipsometry and SPR) and ( b ) 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC) (ellipsometry).

    Journal: Biosensors

    Article Title: Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques

    doi: 10.3390/bios7030034

    Figure Lengend Snippet: Optical response and some schemes of the bilayers measured under heating cycles for ( a ) 1,2-dipalmitoyl- sn -glycero-3-phosphocholine (DPPC) (ellipsometry and SPR) and ( b ) 1,2-distearoyl- sn -glycero-3-phosphocholine (DSPC) (ellipsometry).

    Article Snippet: Materials 1,2-dipalmitoyl-sn -glycero-3-phosphocoline semisynthetic (≥99%, DPPC), 1,2-distearoyl-sn -glycero-3-phosphocholine (≥99%, DSPC), 1,2-dimyristoyl-sn -glycero-3-phosphocholine (≥99%, DMPC) and 1,2-dioleoyl-sn -glycero-3-phosphocholine (DOPC) lyophilized powder were acquired from Sigma-Aldrich Company (St. Louise, MO, USA) and used without further purification.

    Techniques: SPR Assay

    Thermal behavior detected by optical studies (ellipsometry and SPR) with their respective diagrams for ( a ) DPPC: 1,2-dimyristoyl- sn -glycero-3-phosphocholine (DMPC) (2:1) and ( b ) DPPC: 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) (7:1).

    Journal: Biosensors

    Article Title: Thermal Response Analysis of Phospholipid Bilayers Using Ellipsometric Techniques

    doi: 10.3390/bios7030034

    Figure Lengend Snippet: Thermal behavior detected by optical studies (ellipsometry and SPR) with their respective diagrams for ( a ) DPPC: 1,2-dimyristoyl- sn -glycero-3-phosphocholine (DMPC) (2:1) and ( b ) DPPC: 1,2-dioleoyl- sn -glycero-3-phosphocholine (DOPC) (7:1).

    Article Snippet: Materials 1,2-dipalmitoyl-sn -glycero-3-phosphocoline semisynthetic (≥99%, DPPC), 1,2-distearoyl-sn -glycero-3-phosphocholine (≥99%, DSPC), 1,2-dimyristoyl-sn -glycero-3-phosphocholine (≥99%, DMPC) and 1,2-dioleoyl-sn -glycero-3-phosphocholine (DOPC) lyophilized powder were acquired from Sigma-Aldrich Company (St. Louise, MO, USA) and used without further purification.

    Techniques: SPR Assay

    Normalized emission spectra of dyes in solvents and lipid vesicles for So, Lo and Ld phases. Upper panels show normalized emission spectra of laurdan, M-laurdan, MoC-laurdan and C-laurdan in chloroform, Dimethylformamide, ethanol and water (when detectable, i.e. only for Moc-laurdan and C-laurdan). The blue shift seen for the emission spectrum for MoC-laurdan in water is attributed to the fluorescence of non hydrated aggregates, whereas C-laurdan which is highly soluble in water undergoes the expected red shift. Lower panels show emission spectra of laurdan, M-laurdan, MoC-laurdan and C-laurdan in LUVs made of DPPC (So, blue), DOPC/PSM/chol 1:1:1 (Lo, green), DOPC/BSM/chol 1:1:1 (Lo, orange) and DOPC (Ld, red) at 25°C.

    Journal: F1000Research

    Article Title: Characterization of M-laurdan, a versatile probe to explore order in lipid membranes

    doi: 10.12688/f1000research.4805.2

    Figure Lengend Snippet: Normalized emission spectra of dyes in solvents and lipid vesicles for So, Lo and Ld phases. Upper panels show normalized emission spectra of laurdan, M-laurdan, MoC-laurdan and C-laurdan in chloroform, Dimethylformamide, ethanol and water (when detectable, i.e. only for Moc-laurdan and C-laurdan). The blue shift seen for the emission spectrum for MoC-laurdan in water is attributed to the fluorescence of non hydrated aggregates, whereas C-laurdan which is highly soluble in water undergoes the expected red shift. Lower panels show emission spectra of laurdan, M-laurdan, MoC-laurdan and C-laurdan in LUVs made of DPPC (So, blue), DOPC/PSM/chol 1:1:1 (Lo, green), DOPC/BSM/chol 1:1:1 (Lo, orange) and DOPC (Ld, red) at 25°C.

    Article Snippet: Large Unilamellar Vesicles (LUVs) DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and cholesterol were purchased from Sigma-Aldrich.

    Techniques: Fluorescence

    The figure shows the dose-response curve (A) of increasing concentration of H 2 O 2 and mexiletine (10 μM-1 mM) and the cytotoxic effect of increasing concentration (10–100 μM) of mexiletine, its pyrroline derivatives and PP2 (B) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and cell viability (%) is expressed according to the following formula: cell viability (%) = [(test value - blank)/(control value-blank) × 100], where the blank value represents that of cell-free wells, the test value represents that of cells treated with one of the test compounds and the control value represents that of not-treated cells (indicated with dashed line in B ). Each data is from at least 12–38 replicates (wells) and 3–6 different culture dishes. The statistical significance between groups was evaluated by Student's t -test as follow: *significantly different with respect to control value (0.001

    Journal: Frontiers in Pharmacology

    Article Title: Dual Action of Mexiletine and Its Pyrroline Derivatives as Skeletal Muscle Sodium Channel Blockers and Anti-oxidant Compounds: Toward Novel Therapeutic Potential

    doi: 10.3389/fphar.2017.00907

    Figure Lengend Snippet: The figure shows the dose-response curve (A) of increasing concentration of H 2 O 2 and mexiletine (10 μM-1 mM) and the cytotoxic effect of increasing concentration (10–100 μM) of mexiletine, its pyrroline derivatives and PP2 (B) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and cell viability (%) is expressed according to the following formula: cell viability (%) = [(test value - blank)/(control value-blank) × 100], where the blank value represents that of cell-free wells, the test value represents that of cells treated with one of the test compounds and the control value represents that of not-treated cells (indicated with dashed line in B ). Each data is from at least 12–38 replicates (wells) and 3–6 different culture dishes. The statistical significance between groups was evaluated by Student's t -test as follow: *significantly different with respect to control value (0.001

    Article Snippet: Mexiletine was purchased from Sigma-Aldrich (Milan, Italy).

    Techniques: Concentration Assay

    The histograms show the potential cytoprotective effect of increasing concentration of mexiletine, its pyrroline derivatives and PP2 (0.1–30 μM) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and are expressed as the percentage variation of the control according to the following formula: cell viability (%) = [(test value-blank)/(control value-blank)−1] × 100, where the blank value represents that of a cell-free wells, the test value represents that of cells treated either with one of test compounds or H 2 O 2 300 μM and the control value represents that of cells treated with H 2 O 2 300 μM alone. Each data is from at least 12–32 replicates (wells) and 3–8 different culture dishes. All test compounds show a significant cyto-protection with respect to the control value. The statistical significance between groups was found by ANOVA analysis ( F > 5; p

    Journal: Frontiers in Pharmacology

    Article Title: Dual Action of Mexiletine and Its Pyrroline Derivatives as Skeletal Muscle Sodium Channel Blockers and Anti-oxidant Compounds: Toward Novel Therapeutic Potential

    doi: 10.3389/fphar.2017.00907

    Figure Lengend Snippet: The histograms show the potential cytoprotective effect of increasing concentration of mexiletine, its pyrroline derivatives and PP2 (0.1–30 μM) on cell viability of C 2 C 12 cells. Values are presented as the mean ± S.E.M. and are expressed as the percentage variation of the control according to the following formula: cell viability (%) = [(test value-blank)/(control value-blank)−1] × 100, where the blank value represents that of a cell-free wells, the test value represents that of cells treated either with one of test compounds or H 2 O 2 300 μM and the control value represents that of cells treated with H 2 O 2 300 μM alone. Each data is from at least 12–32 replicates (wells) and 3–8 different culture dishes. All test compounds show a significant cyto-protection with respect to the control value. The statistical significance between groups was found by ANOVA analysis ( F > 5; p

    Article Snippet: Mexiletine was purchased from Sigma-Aldrich (Milan, Italy).

    Techniques: Concentration Assay

    The MN promoter-driven cameleons can be efficiently and specifically expressed in MNs after transduction of mice with the AAV vectors in vivo . Neonatal mice (P1) were injected into the temporal vein with AAV vectors encoding the mitochondrial (panels A–C) or the ER (panels D–J) cameleon. Mice were sacrificed 4 weeks after injection, and spinal cord cross-sections were labeled with the nuclear fluorescent dye Hoechst 33342 (panels B,E,I) and immunostained with the MN marker SMI32 (panel H), and then observed with a fluorescence stereo-microscope (panels A–F) or a confocal microscope (panels G–J). Low-resolution stereo-microscopy images show a diffuse expression of both fluorescent Ca 2+ probes (green signal, panels A,D,G), indicating that the cameleons are effectively transduced and expressed in the spinal cord in vivo . The merged reconstruction of complete z-stacks of cells collected by confocal microscopy shows that the D4ER cameleon is expressed only in SMI32-immunopositive MNs (merged image, panel J), suggesting the cell-specific expression of the probe. Scale bars = 200 µm (A–C), 100 µm (D–F), 10 µm (G–J).

    Journal: Scientific Reports

    Article Title: Generation and validation of novel adeno-associated viral vectors for the analysis of Ca2+ homeostasis in motor neurons

    doi: 10.1038/s41598-017-06919-0

    Figure Lengend Snippet: The MN promoter-driven cameleons can be efficiently and specifically expressed in MNs after transduction of mice with the AAV vectors in vivo . Neonatal mice (P1) were injected into the temporal vein with AAV vectors encoding the mitochondrial (panels A–C) or the ER (panels D–J) cameleon. Mice were sacrificed 4 weeks after injection, and spinal cord cross-sections were labeled with the nuclear fluorescent dye Hoechst 33342 (panels B,E,I) and immunostained with the MN marker SMI32 (panel H), and then observed with a fluorescence stereo-microscope (panels A–F) or a confocal microscope (panels G–J). Low-resolution stereo-microscopy images show a diffuse expression of both fluorescent Ca 2+ probes (green signal, panels A,D,G), indicating that the cameleons are effectively transduced and expressed in the spinal cord in vivo . The merged reconstruction of complete z-stacks of cells collected by confocal microscopy shows that the D4ER cameleon is expressed only in SMI32-immunopositive MNs (merged image, panel J), suggesting the cell-specific expression of the probe. Scale bars = 200 µm (A–C), 100 µm (D–F), 10 µm (G–J).

    Article Snippet: Cell nuclei were counter-stained with Hoechst 33342 (5 μg/ml, Sigma), and coverslips were finally washed in PBS, mounted in montage solution [8% Mowiol 40–88 (Sigma) in glycerol and PBS (1:3 (v/v)], and observed with an inverted fluorescence microscope (Leica CTR6000) equipped with a computer-assisted charge-coupled camera (Orca Flash 4.0, Hamamatsu), or with a Leica TSC SP5 inverted confocal microscope system, equipped with a HCX PL APO 63× or 100× magnification, numerical aperture 1.40, oil immersion objective, which also allowed the acquisition and analysis of digital images by a dedicated software (Leica AS).

    Techniques: Transduction, Mouse Assay, In Vivo, Injection, Labeling, Marker, Fluorescence, Microscopy, Expressing, Confocal Microscopy

    The cameleon probes are selectively expressed in motor neurons in mouse spinal cord primary cultures. Primary cell cultures from the mouse spinal cord were transduced with the AAV vectors coding for the cameleons targeted to the cytosol (panels A–D), the mitochondrial matrix (panels E–H) or the ER lumen (panels I–L). After 12 days of culturing, cells were fixed, permeabilised, immunostained with an antibody to the motor neuron (MN) marker SMI32, and then counter-stained with the nuclear fluoro-probe Hoechst 33342. Confocal microscope images of cells were then collected after excitation at either λ = 488 nm for visualising the fluorescent Ca 2+  probes (green signal, panels A,E,I), λ = 543 nm for visualising SMI32-positive cells (red signal, panels B,F,J), or λ = 405 nm for visualising the nuclei of all cells (blue signal, panels C,G,K). Merging of the three fluorescent channels (panels D,H,L) shows that only SMI32-positive MNs express the Ca 2+  probes (see also Supplementary Fig.   S3 ). Scale bar = 20 µm.

    Journal: Scientific Reports

    Article Title: Generation and validation of novel adeno-associated viral vectors for the analysis of Ca2+ homeostasis in motor neurons

    doi: 10.1038/s41598-017-06919-0

    Figure Lengend Snippet: The cameleon probes are selectively expressed in motor neurons in mouse spinal cord primary cultures. Primary cell cultures from the mouse spinal cord were transduced with the AAV vectors coding for the cameleons targeted to the cytosol (panels A–D), the mitochondrial matrix (panels E–H) or the ER lumen (panels I–L). After 12 days of culturing, cells were fixed, permeabilised, immunostained with an antibody to the motor neuron (MN) marker SMI32, and then counter-stained with the nuclear fluoro-probe Hoechst 33342. Confocal microscope images of cells were then collected after excitation at either λ = 488 nm for visualising the fluorescent Ca 2+ probes (green signal, panels A,E,I), λ = 543 nm for visualising SMI32-positive cells (red signal, panels B,F,J), or λ = 405 nm for visualising the nuclei of all cells (blue signal, panels C,G,K). Merging of the three fluorescent channels (panels D,H,L) shows that only SMI32-positive MNs express the Ca 2+ probes (see also Supplementary Fig.  S3 ). Scale bar = 20 µm.

    Article Snippet: Cell nuclei were counter-stained with Hoechst 33342 (5 μg/ml, Sigma), and coverslips were finally washed in PBS, mounted in montage solution [8% Mowiol 40–88 (Sigma) in glycerol and PBS (1:3 (v/v)], and observed with an inverted fluorescence microscope (Leica CTR6000) equipped with a computer-assisted charge-coupled camera (Orca Flash 4.0, Hamamatsu), or with a Leica TSC SP5 inverted confocal microscope system, equipped with a HCX PL APO 63× or 100× magnification, numerical aperture 1.40, oil immersion objective, which also allowed the acquisition and analysis of digital images by a dedicated software (Leica AS).

    Techniques: Transduction, Marker, Staining, Microscopy

    Radiosensitization by ALP-(MIs)n in vitro . (A) Representative images of clonogenic survival assays of C6 cells cultured with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition following treatment with 0, 2, 4, 6 and 8 Gy. (B) Clonogenic survival curves of C6 cells cultured with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition following treatment with 0, 2, 4, 6 and 8 Gy. (C) Immunocytochemical analysis of γ-H2AX expressed by C6 cells. Cells were treated by incubation with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 for 4 h under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition followed by irradiation with 2 Gy using a dose rate of 0.3 Gy min -1 . Cells were stained with an anti-γ-H2AX antibody (red) and DAPI (blue) 24 h after RT. Scale bar, 100 μm. (D) Quantitation of percentage of γ-H2AX positive cells. (Mean ± SD, n = 5, ** p

    Journal: Theranostics

    Article Title: Hypoxia-responsive lipid-poly-(hypoxic radiosensitized polyprodrug) nanoparticles for glioma chemo- and radiotherapy

    doi: 10.7150/thno.26225

    Figure Lengend Snippet: Radiosensitization by ALP-(MIs)n in vitro . (A) Representative images of clonogenic survival assays of C6 cells cultured with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition following treatment with 0, 2, 4, 6 and 8 Gy. (B) Clonogenic survival curves of C6 cells cultured with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition following treatment with 0, 2, 4, 6 and 8 Gy. (C) Immunocytochemical analysis of γ-H2AX expressed by C6 cells. Cells were treated by incubation with AL-PLGA, ALP-(MIs)25, and ALP-(MIs)48 for 4 h under hypoxic condition (pO 2 : 2%) and ALP-(MIs)25 under normoxic condition followed by irradiation with 2 Gy using a dose rate of 0.3 Gy min -1 . Cells were stained with an anti-γ-H2AX antibody (red) and DAPI (blue) 24 h after RT. Scale bar, 100 μm. (D) Quantitation of percentage of γ-H2AX positive cells. (Mean ± SD, n = 5, ** p

    Article Snippet: Poly-lactic-co-glycolic acid (PLGA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT), 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma Aldrich (Saint Louis, MO, USA). γ-H2AX, and Ki67 antibodies were obtained from CellSignaling Technology, Inc. (Danvers, MA, USA).

    Techniques: ALP Assay, In Vitro, Cell Culture, Incubation, Irradiation, Staining, Quantitation Assay

    IC 50 values ( n = 3 ± standard deviation) for the inhibition of pancreatic lipase (PL) activity by orlistat and aqueous polyphenolic extracts of vegetables. Bars with different letters have mean values that are significantly ( p

    Journal: Foods

    Article Title: Inhibitory Activities of Polyphenolic Extracts of Bangladeshi Vegetables against α-Amylase, α-Glucosidase, Pancreatic Lipase, Renin, and Angiotensin-Converting Enzyme

    doi: 10.3390/foods9070844

    Figure Lengend Snippet: IC 50 values ( n = 3 ± standard deviation) for the inhibition of pancreatic lipase (PL) activity by orlistat and aqueous polyphenolic extracts of vegetables. Bars with different letters have mean values that are significantly ( p

    Article Snippet: Porcine pancreatic α-amylase, porcine PL, rat intestinal acetone powder, acarbose, orlistat, 4-nitrophenyl α-D-glucopyranoside (PNP), N-(3-[2-furyl]acryloyl)-phenylalanyl glycyl glycine (FAPGG), and rabbit lung ACE were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Standard Deviation, Inhibition, Activity Assay

    Piperlongumine selectively kills HNC cells (A-B) Piperlongumine induces death in HNC cells but not normal cells. Cytotoxicity was assessed by MTT assay (A), trypan blue exclusion assay, and crystal violet staining (B) after exposure to 1–15 μM piperlongumine (PL) for 48–72 h. Normal human cells (N) included oral keratinocytes (HOK), oral fibroblasts (HOF), and skin keratinocytes (HEK) isolated from human oral mucosa and skin, respectively. The cytotoxic effect of PL was blocked by the antioxidant N -acetyl-L-cystine (NAC, 3 mM). The error bars represent s.d. from three independent experiments, each performed with triplicate samples. * denotes p

    Journal: Oncotarget

    Article Title: Piperlongumine selectively kills cancer cells and increases cisplatin antitumor activity in head and neck cancer

    doi:

    Figure Lengend Snippet: Piperlongumine selectively kills HNC cells (A-B) Piperlongumine induces death in HNC cells but not normal cells. Cytotoxicity was assessed by MTT assay (A), trypan blue exclusion assay, and crystal violet staining (B) after exposure to 1–15 μM piperlongumine (PL) for 48–72 h. Normal human cells (N) included oral keratinocytes (HOK), oral fibroblasts (HOF), and skin keratinocytes (HEK) isolated from human oral mucosa and skin, respectively. The cytotoxic effect of PL was blocked by the antioxidant N -acetyl-L-cystine (NAC, 3 mM). The error bars represent s.d. from three independent experiments, each performed with triplicate samples. * denotes p

    Article Snippet: Measurement of total cellular glutathione and glutathione disulfide To assay total cellular glutathione (GSH), tumor and normal cells were exposed to PL and N-acetyl-L-cysteine (3 mM, NAC, Sigma-Aldrich).

    Techniques: MTT Assay, Trypan Blue Exclusion Assay, Staining, Isolation

    Biodistribution of EGFR-targeted and non-targeted doxorubicin-liposomes in mice bearing i.p. SKOV-3 xenografts. Either targeted liposomal DXR or non-targeted liposomal DXR was injected i.v. at a dose of 2 mg/kg. DXR content was assayed in the indicated tissues at 24 h post-treatment ( n = 3). Data are expressed as mean of % of injected dose (ID)/g tissue, ± SD.

    Journal: PLoS ONE

    Article Title: Pre-Targeting and Direct Immunotargeting of Liposomal Drug Carriers to Ovarian Carcinoma

    doi: 10.1371/journal.pone.0041410

    Figure Lengend Snippet: Biodistribution of EGFR-targeted and non-targeted doxorubicin-liposomes in mice bearing i.p. SKOV-3 xenografts. Either targeted liposomal DXR or non-targeted liposomal DXR was injected i.v. at a dose of 2 mg/kg. DXR content was assayed in the indicated tissues at 24 h post-treatment ( n = 3). Data are expressed as mean of % of injected dose (ID)/g tissue, ± SD.

    Article Snippet: Doxorubicin (DXR, Sigma-Aldrich) was encapsulated into the liposomes at DXR:HSPC, 0.2∶1 (w:w) during incubation at 65°C for 30 min. Free DXR was removed in Sephadex G-50 column equilibrated with 20 mM HEPES/150 mM NaCl (pH 7.4).

    Techniques: Mouse Assay, Injection

    Cytotoxicity of EGFR-targeted and non-targeted doxorubicin-liposomes and free doxorubicin. SKOV-3 (A) and CV-1 (B) cells were exposed to liposomal and free doxorubicin (DXR) (0.3–80 µM) for 2 h. After exposure to the drug, the cells were washed and incubated in growth medium for 5 days. Cell growth was assayed using Alamar Blue®. The data are presented as mean ± SD.

    Journal: PLoS ONE

    Article Title: Pre-Targeting and Direct Immunotargeting of Liposomal Drug Carriers to Ovarian Carcinoma

    doi: 10.1371/journal.pone.0041410

    Figure Lengend Snippet: Cytotoxicity of EGFR-targeted and non-targeted doxorubicin-liposomes and free doxorubicin. SKOV-3 (A) and CV-1 (B) cells were exposed to liposomal and free doxorubicin (DXR) (0.3–80 µM) for 2 h. After exposure to the drug, the cells were washed and incubated in growth medium for 5 days. Cell growth was assayed using Alamar Blue®. The data are presented as mean ± SD.

    Article Snippet: Doxorubicin (DXR, Sigma-Aldrich) was encapsulated into the liposomes at DXR:HSPC, 0.2∶1 (w:w) during incubation at 65°C for 30 min. Free DXR was removed in Sephadex G-50 column equilibrated with 20 mM HEPES/150 mM NaCl (pH 7.4).

    Techniques: Incubation