pkd1 inactivation Search Results


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  • 99
    Qiagen genomic dna mini kit
    Genomic Dna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC formulated dmem f12 medium
    Formulated Dmem F12 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tamoxifen
    Study design. (A) iKsp- Pkd1 del mice, treated with <t>tamoxifen</t> to disrupt the Pkd1 gene at days 38–40. At day 45, sirolimus was started for the short-term (approximately 80 days) and long-term (105–110 days) treatment groups. At 80 days,
    Tamoxifen, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore pkd1
    Active PKD isoforms effectively inhibit cofilin activity and directed cell migration. A , HeLa cells (0.65 × 10 6 cells, 6-cm dish) were co-transfected with cofilin and control vector, constitutively active <t>PKD1,</t> PKD2, or PKD3 as indicated. Lysates
    Pkd1, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    VANGL2 LTD pkd1
    <t>Pkd1</t> and Vangl2 functioned in the same pathway to coordinate BB patch angle and rotational alignment of BBs in E cells. a–d , Whole-mount preparations of the lateral walls of the lateral ventricle at P21 were stained with antibodies against γ-tubulin
    Pkd1, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher gene exp pkd1 mm00465436 g1
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Gene Exp Pkd1 Mm00465436 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    GE Healthcare heat inactivate fbs
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Heat Inactivate Fbs, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Addgene inc large t antigen
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Large T Antigen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Addgene inc trc21 control
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Trc21 Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 84/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    The Jackson Laboratory sm22α promoter
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Sm22α Promoter, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher genomic pcr
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Genomic Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Addgene inc shrna
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Shrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 696 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare sodium pyruvate
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Sodium Pyruvate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare rpmi 1640 media
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Rpmi 1640 Media, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 940 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    New England Biolabs mly1
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Mly1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Addgene inc cre recombinase
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Cre Recombinase, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore ifn inducer polyinosinic polycytidylic acid
    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or <t>eGFP-PKD1-HA</t> constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.
    Ifn Inducer Polyinosinic Polycytidylic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC lncap prostate cancer cells
    Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. <t>LNCaP,</t> <t>C4-2,</t> DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p
    Lncap Prostate Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biochrom heat inactivated fetal calf serum
    Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. <t>LNCaP,</t> <t>C4-2,</t> DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p
    Heat Inactivated Fetal Calf Serum, supplied by Biochrom, used in various techniques. Bioz Stars score: 99/100, based on 454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dexamethasone
    Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. <t>LNCaP,</t> <t>C4-2,</t> DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p
    Dexamethasone, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore egf
    Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. <t>LNCaP,</t> <t>C4-2,</t> DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p
    Egf, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    VANGL2 LTD core pcp component vangl2
    Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. <t>LNCaP,</t> <t>C4-2,</t> DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p
    Core Pcp Component Vangl2, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 98/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Study design. (A) iKsp- Pkd1 del mice, treated with tamoxifen to disrupt the Pkd1 gene at days 38–40. At day 45, sirolimus was started for the short-term (approximately 80 days) and long-term (105–110 days) treatment groups. At 80 days,

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Dose-Dependent Effects of Sirolimus on mTOR Signaling and Polycystic Kidney Disease

    doi: 10.1681/ASN.2011040340

    Figure Lengend Snippet: Study design. (A) iKsp- Pkd1 del mice, treated with tamoxifen to disrupt the Pkd1 gene at days 38–40. At day 45, sirolimus was started for the short-term (approximately 80 days) and long-term (105–110 days) treatment groups. At 80 days,

    Article Snippet: The inducible kidney-specific Pkd1- deletion mouse model (tam-KspCad- CreERT2 ; Pkd1 lox2-11; lox2-11 , referred to as iKsp- Pkd1 del ) and tamoxifen treatments were previously described., The Pkd1 gene was inactivated in male adult mice at postnatal days 38–40 by oral administration of 5 mg of tamoxifen per day (Sigma) for 3 consecutive days.

    Techniques: Mouse Assay

    Active PKD isoforms effectively inhibit cofilin activity and directed cell migration. A , HeLa cells (0.65 × 10 6 cells, 6-cm dish) were co-transfected with cofilin and control vector, constitutively active PKD1, PKD2, or PKD3 as indicated. Lysates

    Journal: The Journal of Biological Chemistry

    Article Title: Protein Kinase D Regulates Cofilin Activity through p21-activated Kinase 4 *

    doi: 10.1074/jbc.M111.259424

    Figure Lengend Snippet: Active PKD isoforms effectively inhibit cofilin activity and directed cell migration. A , HeLa cells (0.65 × 10 6 cells, 6-cm dish) were co-transfected with cofilin and control vector, constitutively active PKD1, PKD2, or PKD3 as indicated. Lysates

    Article Snippet: We and others have shown that PKD1 and PKD2 directly phosphorylate SSH1L at its amino acid residue Ser-978 and that this generates a binding site for 14-3-3 proteins ( A ) ( , ).

    Techniques: Activity Assay, Migration, Transfection, Plasmid Preparation

    PKD regulates PAK4 activity in vivo . A , NMuMG cells (3 × 10 5 , 6-cm dish) were co-transfected with FLAG-tagged PAK4 and either vector control or constitutively active PKD1, PKD2, or PKD3, as indicated. PAK4 was immunoprecipitated (α-FLAG)

    Journal: The Journal of Biological Chemistry

    Article Title: Protein Kinase D Regulates Cofilin Activity through p21-activated Kinase 4 *

    doi: 10.1074/jbc.M111.259424

    Figure Lengend Snippet: PKD regulates PAK4 activity in vivo . A , NMuMG cells (3 × 10 5 , 6-cm dish) were co-transfected with FLAG-tagged PAK4 and either vector control or constitutively active PKD1, PKD2, or PKD3, as indicated. PAK4 was immunoprecipitated (α-FLAG)

    Article Snippet: We and others have shown that PKD1 and PKD2 directly phosphorylate SSH1L at its amino acid residue Ser-978 and that this generates a binding site for 14-3-3 proteins ( A ) ( , ).

    Techniques: Activity Assay, In Vivo, Transfection, Plasmid Preparation, Immunoprecipitation

    Active PKD isoforms phosphorylate and inactivate SSH1L. A ). B , HeLa cells (0.5 × 10 6 , 6-cm dish) were transfected with tagged, constitutively active alleles of PKD1, PKD2,

    Journal: The Journal of Biological Chemistry

    Article Title: Protein Kinase D Regulates Cofilin Activity through p21-activated Kinase 4 *

    doi: 10.1074/jbc.M111.259424

    Figure Lengend Snippet: Active PKD isoforms phosphorylate and inactivate SSH1L. A ). B , HeLa cells (0.5 × 10 6 , 6-cm dish) were transfected with tagged, constitutively active alleles of PKD1, PKD2,

    Article Snippet: We and others have shown that PKD1 and PKD2 directly phosphorylate SSH1L at its amino acid residue Ser-978 and that this generates a binding site for 14-3-3 proteins ( A ) ( , ).

    Techniques: Transfection

    PKD regulates activity of the PAK4 substrate LIMK1. A , NMuMG cells (5 × 10 5 , 6-cm dish) were co-transfected with FLAG-tagged LIMK or active PKD1 or respective controls. LIMK was immunoprecipitated (α-FLAG) and analyzed for phosphorylation

    Journal: The Journal of Biological Chemistry

    Article Title: Protein Kinase D Regulates Cofilin Activity through p21-activated Kinase 4 *

    doi: 10.1074/jbc.M111.259424

    Figure Lengend Snippet: PKD regulates activity of the PAK4 substrate LIMK1. A , NMuMG cells (5 × 10 5 , 6-cm dish) were co-transfected with FLAG-tagged LIMK or active PKD1 or respective controls. LIMK was immunoprecipitated (α-FLAG) and analyzed for phosphorylation

    Article Snippet: We and others have shown that PKD1 and PKD2 directly phosphorylate SSH1L at its amino acid residue Ser-978 and that this generates a binding site for 14-3-3 proteins ( A ) ( , ).

    Techniques: Activity Assay, Transfection, Immunoprecipitation

    Active PKD and active PAK4 localize to the leading edge. HuMEC cells (1 × 10 4 cells, 8-well ibiTreat μ-slide) were co-transfected with FLAG-tagged PAK4 and either empty vector or GFP-tagged constitutively active PKD1 (PKD1.CA) as indicated,

    Journal: The Journal of Biological Chemistry

    Article Title: Protein Kinase D Regulates Cofilin Activity through p21-activated Kinase 4 *

    doi: 10.1074/jbc.M111.259424

    Figure Lengend Snippet: Active PKD and active PAK4 localize to the leading edge. HuMEC cells (1 × 10 4 cells, 8-well ibiTreat μ-slide) were co-transfected with FLAG-tagged PAK4 and either empty vector or GFP-tagged constitutively active PKD1 (PKD1.CA) as indicated,

    Article Snippet: We and others have shown that PKD1 and PKD2 directly phosphorylate SSH1L at its amino acid residue Ser-978 and that this generates a binding site for 14-3-3 proteins ( A ) ( , ).

    Techniques: Transfection, Plasmid Preparation

    Pkd1 and Vangl2 functioned in the same pathway to coordinate BB patch angle and rotational alignment of BBs in E cells. a–d , Whole-mount preparations of the lateral walls of the lateral ventricle at P21 were stained with antibodies against γ-tubulin

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Pkd1 and Vangl2 functioned in the same pathway to coordinate BB patch angle and rotational alignment of BBs in E cells. a–d , Whole-mount preparations of the lateral walls of the lateral ventricle at P21 were stained with antibodies against γ-tubulin

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Staining

    Primary cilia, Pkd1 , and Pkd2 were involved in the establishment of translational polarity in RGCs. a , Schematic drawing showing BB patch angle and BB patch displacement. A vector (red arrow) was drawn from the center of apical surface to that of BB patch

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Primary cilia, Pkd1 , and Pkd2 were involved in the establishment of translational polarity in RGCs. a , Schematic drawing showing BB patch angle and BB patch displacement. A vector (red arrow) was drawn from the center of apical surface to that of BB patch

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Plasmid Preparation

    Differentiation of E cells was largely normal in Nestin-Cre;Pkd1 flox/flox and Nestin-Cre;Pkd2 flox/flox mice. a–d , The pinwheel organization of E cells surrounding B1 cells' apical endings was preserved in Nestin-Cre;Pkd1 flox/flox and Nestin-Cre;Pkd2

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Differentiation of E cells was largely normal in Nestin-Cre;Pkd1 flox/flox and Nestin-Cre;Pkd2 flox/flox mice. a–d , The pinwheel organization of E cells surrounding B1 cells' apical endings was preserved in Nestin-Cre;Pkd1 flox/flox and Nestin-Cre;Pkd2

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Mouse Assay

    Primary cilia of RGCs, Pkd1 , and Pkd2 were required for the asymmetric localization of Vangl2. a–l , Whole-mount preparations of the lateral walls of the lateral ventricle were stained with antibodies against Vangl2 (green) and β-catenin

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Primary cilia of RGCs, Pkd1 , and Pkd2 were required for the asymmetric localization of Vangl2. a–l , Whole-mount preparations of the lateral walls of the lateral ventricle were stained with antibodies against Vangl2 (green) and β-catenin

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Staining

    Pkd1 and Pkd2 colocalized in RGCs' primary cilia. Whole-mount preparations of the lateral walls of lateral ventricle from Pkd1 flox/flox ( a – d , i ), Nestin-Cre;Pkd1 flox/flox ( e – h , j ), Pkd2 flox/flox ( k ), and Nestin-Cre;Pkd2 flox/flox ( l ) at

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Pkd1 and Pkd2 colocalized in RGCs' primary cilia. Whole-mount preparations of the lateral walls of lateral ventricle from Pkd1 flox/flox ( a – d , i ), Nestin-Cre;Pkd1 flox/flox ( e – h , j ), Pkd2 flox/flox ( k ), and Nestin-Cre;Pkd2 flox/flox ( l ) at

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques:

    Synergistic enhancement of PCP defects in the Vangl2 heterozygous mutant E cells by Pkd1 heterozygous mutation

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Synergistic enhancement of PCP defects in the Vangl2 heterozygous mutant E cells by Pkd1 heterozygous mutation

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Mutagenesis

    Pkd1 and Pkd2 localized to RGCs' primary cilia. Whole-mount preparations of the lateral walls of lateral ventricle from Pkd1 flox/flox ( a ), WT ( b , d , e , h ), Nestin-Cre;Pkd1 flox/flox ( c ), Pkd2 flox/flox ( f ), and Nestin-Cre;Pkd2 flox/flox ( g ) at P0 were stained

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Pkd1 and Pkd2 localized to RGCs' primary cilia. Whole-mount preparations of the lateral walls of lateral ventricle from Pkd1 flox/flox ( a ), WT ( b , d , e , h ), Nestin-Cre;Pkd1 flox/flox ( c ), Pkd2 flox/flox ( f ), and Nestin-Cre;Pkd2 flox/flox ( g ) at P0 were stained

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Staining

    Pkd1 and Pkd2 were expressed in microdissected lateral walls of the lateral ventricle. a , Schematic drawing of the lateral wall of the lateral ventricle at P0. The red dotted line indicates the border between the anterior and posterior regions used for

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Pkd1 and Pkd2 were expressed in microdissected lateral walls of the lateral ventricle. a , Schematic drawing of the lateral wall of the lateral ventricle at P0. The red dotted line indicates the border between the anterior and posterior regions used for

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques:

    Pkd1 and Pkd2 contributed to translational polarity in E cells. a–f , Histograms showing the distribution of the BB patch angles in the Pkd1 flox/flox ( a – e , black), Nestin-Cre;Pkd1 flox/flox ( a – e , red), Pkd2 flox/flox ( f , black), and

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Pkd1 and Pkd2 contributed to translational polarity in E cells. a–f , Histograms showing the distribution of the BB patch angles in the Pkd1 flox/flox ( a – e , black), Nestin-Cre;Pkd1 flox/flox ( a – e , red), Pkd2 flox/flox ( f , black), and

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques:

    Rotational polarity was disrupted in the Nestin-Cre;Pkd1 flox/flox and Nestin-Cre;Pkd2 flox/flox E cells. a–f , TEM analysis of rotational polarity in E cells in the anterior-dorsal region in Pkd1 flox/flox ( a ), Nestin-Cre;Pkd1 flox/flox ( b ), Pkd2

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Rotational polarity was disrupted in the Nestin-Cre;Pkd1 flox/flox and Nestin-Cre;Pkd2 flox/flox E cells. a–f , TEM analysis of rotational polarity in E cells in the anterior-dorsal region in Pkd1 flox/flox ( a ), Nestin-Cre;Pkd1 flox/flox ( b ), Pkd2

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Transmission Electron Microscopy

    Synergistic enhancement of PCP defects in the Vangl2 heterozygous mutant E cells by Pkd1 heterozygous mutation

    Journal: The Journal of Neuroscience

    Article Title: Mechanosensory Genes Pkd1 and Pkd2 Contribute to the Planar Polarization of Brain Ventricular Epithelium

    doi: 10.1523/JNEUROSCI.0686-15.2015

    Figure Lengend Snippet: Synergistic enhancement of PCP defects in the Vangl2 heterozygous mutant E cells by Pkd1 heterozygous mutation

    Article Snippet: [ ] [ ] Shillingford JM, Piontek KB, Germino GG, Weimbs T. Rapamycin ameliorates PKD resulting from conditional inactivation of Pkd1.

    Techniques: Mutagenesis

    Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or eGFP-PKD1-HA constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.

    Journal: Scientific Reports

    Article Title: A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed

    doi: 10.1038/s41598-018-20856-6

    Figure Lengend Snippet: Recombinant PC1 localizes to mitochondria in a subset of cells. ( a . The CTF can be further processed to release a cytoplasmic tail (CTT). Constructs used in the study include full-length human PC1 (hFL) with N-terminal (eGFP or mCherry) and C-terminal (HA or eGFP) tags. ( b ) Lysates from MDCK cells with either stable, inducible expression of vector control (pcDNA5) or eGFP-PKD1-HA constructs probed with GFP or HA antibodies show that the majority of cellular PC1 is free NTF, with only a small amount of uncleaved FL and CTF. ( c – f ) NIH3T3 cells transfected with mCherry-PKD1-eGFP show three main patterns: (1) NTF in the ER with undetectable CTF ( c ); (2) FL and/or NTF/CTF either mostly co-localized in the ER ( d ) or with partial co-localization in the ER and with CTF/CTT in distinct subcellular regions ( e , panel insert); (3) NTF localized to the ER and CTF or CTT in mitochondria ( f ). The endoplasmic reticulum (ER) is identified by transient expression of pEF.myc.ER-E2-Crimson or stained with ER-Tracker™ Blue-White DPX ( c , d ) and mitochondria (Mito) are identified by transient expression of mito-BFP or stained with MitoTracker Deep Red.

    Article Snippet: Pkd1 inactivation was confirmed using genomic PCR and/or reverse-transcriptase PCR (TaqMan gene expression assay, Applied Biosystems, cat. no. 4351372, Mm00465436_g1).

    Techniques: Recombinant, Construct, Expressing, Plasmid Preparation, Transfection, Staining

    Pkd1 ko/ko cells have differences in mitochondrial function and morphology. ( a ) Representative distribution of mitochondrial membrane potential in 96784-LTL cells measured by flow cytometry and showing increased frequency of mutant cells with higher TMRM fluorescence. ( b ) Curves of the difference between mutant and wild type TMRM fluorescence for each centile of the TMRM distribution, showing that mutant cells tend to have higher TMRM intensity, particularly in the upper centiles. Each dotted line corresponds to one experiment, colored by cell line. The blue solid line is the best fit of the data. ( c ) P-values comparing TMRM intensity at each centile and showing that mutant cells have significantly higher TMRM for cells in the upper two thirds of the TMRM distribution. Red line: p = 0.05, n = 10. ( d ) Representative image showing 96784-LTL control and Pkd1 ko/ko cells stained with mitochondrial marker (gray: MitoTracker Deep Red; blue: Hoechest 33342 nuclear stain). The panels on the right show higher magnification of the areas inside the red squares. Mitochondria form a more elongated and interconnected network in controls. ( e ) Representative cumulative distribution in 96784-LTL control (blue line) and Pkd1 ko/ko cells showing that at most centiles (y-axis) the solidity is higher (i.e. mitochondrial network is more fragmented) in mutants (red line). ( f ) Measure of mitochondrial fragmentation in independent experiments comparing three matched mutant and control cell lines. The y-axis shows the 25 th percentile solidity (higher values correspond to more fragmented mitochondrial network). Lines connect matched control (left) and mutant (right) for each independent experiment (n = 13 experiments; average number of analyzed mitochondria/dot: 799; p-value

    Journal: Scientific Reports

    Article Title: A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed

    doi: 10.1038/s41598-018-20856-6

    Figure Lengend Snippet: Pkd1 ko/ko cells have differences in mitochondrial function and morphology. ( a ) Representative distribution of mitochondrial membrane potential in 96784-LTL cells measured by flow cytometry and showing increased frequency of mutant cells with higher TMRM fluorescence. ( b ) Curves of the difference between mutant and wild type TMRM fluorescence for each centile of the TMRM distribution, showing that mutant cells tend to have higher TMRM intensity, particularly in the upper centiles. Each dotted line corresponds to one experiment, colored by cell line. The blue solid line is the best fit of the data. ( c ) P-values comparing TMRM intensity at each centile and showing that mutant cells have significantly higher TMRM for cells in the upper two thirds of the TMRM distribution. Red line: p = 0.05, n = 10. ( d ) Representative image showing 96784-LTL control and Pkd1 ko/ko cells stained with mitochondrial marker (gray: MitoTracker Deep Red; blue: Hoechest 33342 nuclear stain). The panels on the right show higher magnification of the areas inside the red squares. Mitochondria form a more elongated and interconnected network in controls. ( e ) Representative cumulative distribution in 96784-LTL control (blue line) and Pkd1 ko/ko cells showing that at most centiles (y-axis) the solidity is higher (i.e. mitochondrial network is more fragmented) in mutants (red line). ( f ) Measure of mitochondrial fragmentation in independent experiments comparing three matched mutant and control cell lines. The y-axis shows the 25 th percentile solidity (higher values correspond to more fragmented mitochondrial network). Lines connect matched control (left) and mutant (right) for each independent experiment (n = 13 experiments; average number of analyzed mitochondria/dot: 799; p-value

    Article Snippet: Pkd1 inactivation was confirmed using genomic PCR and/or reverse-transcriptase PCR (TaqMan gene expression assay, Applied Biosystems, cat. no. 4351372, Mm00465436_g1).

    Techniques: Flow Cytometry, Cytometry, Mutagenesis, Fluorescence, Staining, Marker

    . Alternatively, PC1-CTT may directly change mitochondrial fusion/fission rates, changing the mitochondrial network and function. How these changes would result in cystic change is not clear, but as discussed in the text, a similar cascade was observed in lymphangiogenesis and planar cell polarity, processes previously linked to Pkd1 . This model does not include PC2 or regulation of NTF/CTF dissociation, aspects likely relevant, but not investigated in this study.

    Journal: Scientific Reports

    Article Title: A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed

    doi: 10.1038/s41598-018-20856-6

    Figure Lengend Snippet: . Alternatively, PC1-CTT may directly change mitochondrial fusion/fission rates, changing the mitochondrial network and function. How these changes would result in cystic change is not clear, but as discussed in the text, a similar cascade was observed in lymphangiogenesis and planar cell polarity, processes previously linked to Pkd1 . This model does not include PC2 or regulation of NTF/CTF dissociation, aspects likely relevant, but not investigated in this study.

    Article Snippet: Pkd1 inactivation was confirmed using genomic PCR and/or reverse-transcriptase PCR (TaqMan gene expression assay, Applied Biosystems, cat. no. 4351372, Mm00465436_g1).

    Techniques:

    Pkd1 ko/ko cells have metabolic differences. ( a ) Fluxomics. Principal components bi-plot showing clustering of three replicates of a mutant and control immortalized kidney epithelial cell line (94414-LTL) according to flux of 13 C from labeled glucose through different metabolites. Circles are samples, and their location in the plot is determined by a linear combination of specific factors (metabolites). The direction and weight each metabolite contributes to the location of the sample is represented by the direction and size of the corresponding arrow. Mutant (red circles) and control (blue circles) samples cluster in opposite corners of the figure, and labeled arrows show the metabolites that have the highest influence in separating groups. ( b ) Fatty acid uptake assay showing that mutant cells have increased number and size of lipid droplets (green: mitochondria stained with MitoTracker Green; Magenta: BODIPY 558/568 C 12 ). The panels on the right show higher magnification of the areas inside the white squares. ( c ) Quantile plot showing distribution of lipid droplet size quantified in ten random fields in two proximal tubule kidney cell lines (each line is one experiment for one cell line). The insert on the left shows only up to the 80 th quantile, to highlight differences within the lower range of area values (n = 4 experiments, p = 0.044; line: percentile values of the lipid droplet area for one experiment, colored by genotype).

    Journal: Scientific Reports

    Article Title: A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed

    doi: 10.1038/s41598-018-20856-6

    Figure Lengend Snippet: Pkd1 ko/ko cells have metabolic differences. ( a ) Fluxomics. Principal components bi-plot showing clustering of three replicates of a mutant and control immortalized kidney epithelial cell line (94414-LTL) according to flux of 13 C from labeled glucose through different metabolites. Circles are samples, and their location in the plot is determined by a linear combination of specific factors (metabolites). The direction and weight each metabolite contributes to the location of the sample is represented by the direction and size of the corresponding arrow. Mutant (red circles) and control (blue circles) samples cluster in opposite corners of the figure, and labeled arrows show the metabolites that have the highest influence in separating groups. ( b ) Fatty acid uptake assay showing that mutant cells have increased number and size of lipid droplets (green: mitochondria stained with MitoTracker Green; Magenta: BODIPY 558/568 C 12 ). The panels on the right show higher magnification of the areas inside the white squares. ( c ) Quantile plot showing distribution of lipid droplet size quantified in ten random fields in two proximal tubule kidney cell lines (each line is one experiment for one cell line). The insert on the left shows only up to the 80 th quantile, to highlight differences within the lower range of area values (n = 4 experiments, p = 0.044; line: percentile values of the lipid droplet area for one experiment, colored by genotype).

    Article Snippet: Pkd1 inactivation was confirmed using genomic PCR and/or reverse-transcriptase PCR (TaqMan gene expression assay, Applied Biosystems, cat. no. 4351372, Mm00465436_g1).

    Techniques: Mutagenesis, Labeling, Staining

    PC1-CTT levels are not altered by cellular stress or inhibition of degradation pathways. ( a – c ) Immunoblot of MDCK cells with stable, inducible expression of PKD1 (eGFP-PKD1-HA) or pcDNA5. PC1-FL and PC1-CTT amounts are unchanged after ( a ) treatment with 1 μM CCCP for 15 min or 18 hours; ( b ) 2 h hypoxia (0.01% O 2 , 5% CO 2 ); or ( c ). ( d , e ) 24 h treatment with protease inhibitors ( d ) or γ-secretase inhibitor ( e ) had no effect on PC1-CTT detection.

    Journal: Scientific Reports

    Article Title: A cleavage product of Polycystin-1 is a mitochondrial matrix protein that affects mitochondria morphology and function when heterologously expressed

    doi: 10.1038/s41598-018-20856-6

    Figure Lengend Snippet: PC1-CTT levels are not altered by cellular stress or inhibition of degradation pathways. ( a – c ) Immunoblot of MDCK cells with stable, inducible expression of PKD1 (eGFP-PKD1-HA) or pcDNA5. PC1-FL and PC1-CTT amounts are unchanged after ( a ) treatment with 1 μM CCCP for 15 min or 18 hours; ( b ) 2 h hypoxia (0.01% O 2 , 5% CO 2 ); or ( c ). ( d , e ) 24 h treatment with protease inhibitors ( d ) or γ-secretase inhibitor ( e ) had no effect on PC1-CTT detection.

    Article Snippet: Pkd1 inactivation was confirmed using genomic PCR and/or reverse-transcriptase PCR (TaqMan gene expression assay, Applied Biosystems, cat. no. 4351372, Mm00465436_g1).

    Techniques: Inhibition, Expressing

    Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. LNCaP, C4-2, DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p

    Journal: PLoS ONE

    Article Title: Curcumin Attenuates ?-catenin Signaling in Prostate Cancer Cells through Activation of Protein Kinase D1

    doi: 10.1371/journal.pone.0035368

    Figure Lengend Snippet: Curcumin inhibits prostate cancer cell proliferation. A). Chemical structure of curcumin. B). Effect of curcumin on proliferation of various prostate cancer cell lines. LNCaP, C4-2, DU145 and PC3 cell were treated with curcumin or vehicle control DMSO for 48 h and cell proliferation was determined using MTS assay. The percent cell proliferation was calculated by normalizing the proliferation of curcumin treated cells with proliferation of control treated cells. Concentration dependent inhibition in cell proliferation was observed with curcumin treatment. Mean ± SE; n = 3; *p

    Article Snippet: C4-2 (UroCor, Oklahoma City, OK), PC3, and LNCaP prostate cancer cells (ATCC, Manassas, Virginia) and C4-2 cells overexpressing PKD1 were maintained in RPMI 1640 media containing 1 mM sodium pyruvate, 2.5 mM glutamine, 10% heat-inactivate FBS (Hyclone) and 1× antibiotic and antimycotic solution.

    Techniques: MTS Assay, Concentration Assay, Inhibition