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  • 99
    New England Biolabs nebuilder hifi dna assembly master mix
    Nebuilder Hifi Dna Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC b laterosporus atcc 9141
    Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. <t>laterosporus</t> <t>ATCC</t> 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments
    B Laterosporus Atcc 9141, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    b laterosporus atcc 9141 - by Bioz Stars, 2020-07
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    92
    ATCC b laterosporus atcc 9141 chromosomal dna
    Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. <t>laterosporus</t> <t>ATCC</t> 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments
    B Laterosporus Atcc 9141 Chromosomal Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b laterosporus atcc 9141 chromosomal dna/product/ATCC
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    93
    ATCC polymerase chain reaction pcr
    Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. <t>laterosporus</t> <t>ATCC</t> 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments
    Polymerase Chain Reaction Pcr, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr/product/ATCC
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    Image Search Results


    Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. laterosporus ATCC 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments

    Journal: Nature Communications

    Article Title: Discovery of cationic nonribosomal peptides as Gram-negative antibiotics through global genome mining

    doi: 10.1038/s41467-018-05781-6

    Figure Lengend Snippet: Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. laterosporus ATCC 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments

    Article Snippet: Plasmid pJOE8999-latC was transformed into B. laterosporus ATCC 9141 according to a modified electroporation method .

    Techniques: Isolation, Mass Spectrometry, Nuclear Magnetic Resonance, Liquid Chromatography, Mutagenesis

    Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. laterosporus ATCC 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments

    Journal: Nature Communications

    Article Title: Discovery of cationic nonribosomal peptides as Gram-negative antibiotics through global genome mining

    doi: 10.1038/s41467-018-05781-6

    Figure Lengend Snippet: Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. laterosporus ATCC 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments

    Article Snippet: In the second step, two fragments flanking latC ~400 bp in length were amplified by polymerase chain reaction (PCR) using primers LatC -Up-F/R and LatC -41-Do-F/R from B. laterosporus ATCC 9141 chromosomal DNA as the template, and then cut with SfiI and inserted between the two SfiI sites to give pJOE8999-latC .

    Techniques: Isolation, Mass Spectrometry, Nuclear Magnetic Resonance, Liquid Chromatography, Mutagenesis