Journal: Nature Communications
Article Title: Discovery of cationic nonribosomal peptides as Gram-negative antibiotics through global genome mining
Figure Lengend Snippet: Cationic antibacterial peptides discovered via global genome mining. a Two gene clusters of investigated cationic peptides, with amino acids incorporated. Cationic residues are marked in red. b Structure of isolated cationic peptides identified by MS/NMR analysis. Positively charged amino acids are highlighted in red. c Mass spectra (ESI) of cationic peptide brevicidine. d Liquid chromatography-mass spectrometry traces comparing B. laterosporus ATCC 9141 wild type and the Δ latC mutant. Figure 3 c – d are representative of three independent experiments
Article Snippet: In the second step, two fragments flanking latC ~400 bp in length were amplified by polymerase chain reaction (PCR) using primers LatC -Up-F/R and LatC -41-Do-F/R from B. laterosporus ATCC 9141 chromosomal DNA as the template, and then cut with SfiI and inserted between the two SfiI sites to give pJOE8999-latC .
Techniques: Isolation, Mass Spectrometry, Nuclear Magnetic Resonance, Liquid Chromatography, Mutagenesis