pig intestinal Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Tocris vip treatment
    A, Viable bacteria detected by direct plate counts from corneas of PBS- and <t>VIP-treated</t> B6 mice at 3 and 5 days p.i. Results are reported as CFU/cornea ± SEM (n = 6 corneas/group). B, Estimation of PMN as calculated from MPO levels detected from corneas of PBS- and VIP-treated B6 mice after infection. Results are reported as log10/cornea + SEM (n = 5 corneas/group). C, Nitrite levels from infected corneas after PBS and <t>VIP</t> <t>treatment.</t> Data are reported as μM/cornea ± SEM (n = 5 corneas/group).
    Vip Treatment, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vip treatment/product/Tocris
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vip treatment - by Bioz Stars, 2024-06
    93/100 stars
      Buy from Supplier

    86
    Millipore pig intestine
    A, Viable bacteria detected by direct plate counts from corneas of PBS- and <t>VIP-treated</t> B6 mice at 3 and 5 days p.i. Results are reported as CFU/cornea ± SEM (n = 6 corneas/group). B, Estimation of PMN as calculated from MPO levels detected from corneas of PBS- and VIP-treated B6 mice after infection. Results are reported as log10/cornea + SEM (n = 5 corneas/group). C, Nitrite levels from infected corneas after PBS and <t>VIP</t> <t>treatment.</t> Data are reported as μM/cornea ± SEM (n = 5 corneas/group).
    Pig Intestine, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pig intestine/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pig intestine - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    DSMZ pig intestine
    A, Viable bacteria detected by direct plate counts from corneas of PBS- and <t>VIP-treated</t> B6 mice at 3 and 5 days p.i. Results are reported as CFU/cornea ± SEM (n = 6 corneas/group). B, Estimation of PMN as calculated from MPO levels detected from corneas of PBS- and VIP-treated B6 mice after infection. Results are reported as log10/cornea + SEM (n = 5 corneas/group). C, Nitrite levels from infected corneas after PBS and <t>VIP</t> <t>treatment.</t> Data are reported as μM/cornea ± SEM (n = 5 corneas/group).
    Pig Intestine, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pig intestine/product/DSMZ
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pig intestine - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    inTEST Corporation pig intestinal smcs
    A: Phase-contrast micrographs of 3-day-old cultures, illustrating that the growth rate of Hurler fibroblasts was considerably higher than those taken from normal children. Whereas the addition of insoluble elastin (arrows) to cultured normal fibroblasts did not affect their final density, addition of insoluble elastin (arrows) to cultures of Hurler fibroblasts substantially reduced their number in 3-day-old culture. B: Incorporation of 3H-thymidine also indicates higher growth of Hurler fibroblasts as compared with normal and Sanfilippo fibroblasts in 3-day-old cultures. These assays also illustrate that high proliferation of Hurler fibroblasts was significantly reduced in cultures treated with exogenous insoluble elastin (striated bars). C: Addition of DS, but not HS (both in concentrations of 400 μg/ml) significantly increased incorporation of 3H-thymidine to normal and Sanfilippo fibroblasts. Hurler fibroblasts cultured for 3 days in the presence of chondroitinase ABC (0.2 U/day) (Chnase ABC) decrease their 3H-thymidine incorporation. D: <t>Pig</t> <t>intestinal</t> <t>SMCs</t> (INTEST SMC), which do not produce elastin, incorporate more radioactive thymidine then the elastin-producing pig coronary artery SMCs (CA SMC). CA SMCs incubated for 3 days in the presence of 400 μg/ml of DS, up-regulate their incorporation of labeled thymidine, as compared with the untreated control and cells incubated with HS. In contrast, neither DS nor HS affected proliferation of pig intestinal SMCs. In all experiments, cells were plated with the same initial density 50,000 cells/well. Values of mean ± SD from 3 different experiments were statistically compared with untreated controls within the same cell type (*P < 0.002). Proliferation rates of different cell types tested were also assessed (**P < 0.05).
    Pig Intestinal Smcs, supplied by inTEST Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pig intestinal smcs/product/inTEST Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pig intestinal smcs - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Celsus Laboratories pig intestinal mucosa
    A: Phase-contrast micrographs of 3-day-old cultures, illustrating that the growth rate of Hurler fibroblasts was considerably higher than those taken from normal children. Whereas the addition of insoluble elastin (arrows) to cultured normal fibroblasts did not affect their final density, addition of insoluble elastin (arrows) to cultures of Hurler fibroblasts substantially reduced their number in 3-day-old culture. B: Incorporation of 3H-thymidine also indicates higher growth of Hurler fibroblasts as compared with normal and Sanfilippo fibroblasts in 3-day-old cultures. These assays also illustrate that high proliferation of Hurler fibroblasts was significantly reduced in cultures treated with exogenous insoluble elastin (striated bars). C: Addition of DS, but not HS (both in concentrations of 400 μg/ml) significantly increased incorporation of 3H-thymidine to normal and Sanfilippo fibroblasts. Hurler fibroblasts cultured for 3 days in the presence of chondroitinase ABC (0.2 U/day) (Chnase ABC) decrease their 3H-thymidine incorporation. D: <t>Pig</t> <t>intestinal</t> <t>SMCs</t> (INTEST SMC), which do not produce elastin, incorporate more radioactive thymidine then the elastin-producing pig coronary artery SMCs (CA SMC). CA SMCs incubated for 3 days in the presence of 400 μg/ml of DS, up-regulate their incorporation of labeled thymidine, as compared with the untreated control and cells incubated with HS. In contrast, neither DS nor HS affected proliferation of pig intestinal SMCs. In all experiments, cells were plated with the same initial density 50,000 cells/well. Values of mean ± SD from 3 different experiments were statistically compared with untreated controls within the same cell type (*P < 0.002). Proliferation rates of different cell types tested were also assessed (**P < 0.05).
    Pig Intestinal Mucosa, supplied by Celsus Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pig intestinal mucosa/product/Celsus Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pig intestinal mucosa - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    A, Viable bacteria detected by direct plate counts from corneas of PBS- and VIP-treated B6 mice at 3 and 5 days p.i. Results are reported as CFU/cornea ± SEM (n = 6 corneas/group). B, Estimation of PMN as calculated from MPO levels detected from corneas of PBS- and VIP-treated B6 mice after infection. Results are reported as log10/cornea + SEM (n = 5 corneas/group). C, Nitrite levels from infected corneas after PBS and VIP treatment. Data are reported as μM/cornea ± SEM (n = 5 corneas/group).

    Journal: Prostaglandins & other lipid mediators

    Article Title: VIP Modulates the ALX/FPR2 Receptor Axis toward Inflammation Resolution in a Mouse Model of Bacterial Keratitis

    doi: 10.1016/j.prostaglandins.2018.12.001

    Figure Lengend Snippet: A, Viable bacteria detected by direct plate counts from corneas of PBS- and VIP-treated B6 mice at 3 and 5 days p.i. Results are reported as CFU/cornea ± SEM (n = 6 corneas/group). B, Estimation of PMN as calculated from MPO levels detected from corneas of PBS- and VIP-treated B6 mice after infection. Results are reported as log10/cornea + SEM (n = 5 corneas/group). C, Nitrite levels from infected corneas after PBS and VIP treatment. Data are reported as μM/cornea ± SEM (n = 5 corneas/group).

    Article Snippet: The cells were treated +/− select receptor antagonists, WRW4 (Tocris, Minneapolis, MN) and [D-p-Cl-Phe 6 ,Leu 17 ]-VIP (Leu) (Tocris) (specific for FPR2 and VIPR, respectively) for 24 hours prior to VIP treatment and PA stimulation.

    Techniques: Infection

    Select pro-inflammatory cytokine/chemokine transcript levels were assessed after VIP treatment in infected corneas of B6 mice. Significant decreases were observed in iNOS (A), MIP-2 (B), IL-1β (C), and TNF-α (D) mRNA levels in VIP- versus PBS-treated mice. Results are presented as relative fold change for the gene of interest normalized to β-actin ± SEM (n = 5 corneas/group).

    Journal: Prostaglandins & other lipid mediators

    Article Title: VIP Modulates the ALX/FPR2 Receptor Axis toward Inflammation Resolution in a Mouse Model of Bacterial Keratitis

    doi: 10.1016/j.prostaglandins.2018.12.001

    Figure Lengend Snippet: Select pro-inflammatory cytokine/chemokine transcript levels were assessed after VIP treatment in infected corneas of B6 mice. Significant decreases were observed in iNOS (A), MIP-2 (B), IL-1β (C), and TNF-α (D) mRNA levels in VIP- versus PBS-treated mice. Results are presented as relative fold change for the gene of interest normalized to β-actin ± SEM (n = 5 corneas/group).

    Article Snippet: The cells were treated +/− select receptor antagonists, WRW4 (Tocris, Minneapolis, MN) and [D-p-Cl-Phe 6 ,Leu 17 ]-VIP (Leu) (Tocris) (specific for FPR2 and VIPR, respectively) for 24 hours prior to VIP treatment and PA stimulation.

    Techniques: Infection

    Levels of RvD1 (A) and FPR2 (B) as detected by ELISA and Western blot (respectively) in infected corneas of PBS- and VIP-treated B6 mice. Results are reported as mean (pg/cornea) ± SEM for ELISA analysis. For Western blot, a representative image is provided from three independent experiments in duplicate and expressed as mean ± SEM (n = 6 corneas/group).

    Journal: Prostaglandins & other lipid mediators

    Article Title: VIP Modulates the ALX/FPR2 Receptor Axis toward Inflammation Resolution in a Mouse Model of Bacterial Keratitis

    doi: 10.1016/j.prostaglandins.2018.12.001

    Figure Lengend Snippet: Levels of RvD1 (A) and FPR2 (B) as detected by ELISA and Western blot (respectively) in infected corneas of PBS- and VIP-treated B6 mice. Results are reported as mean (pg/cornea) ± SEM for ELISA analysis. For Western blot, a representative image is provided from three independent experiments in duplicate and expressed as mean ± SEM (n = 6 corneas/group).

    Article Snippet: The cells were treated +/− select receptor antagonists, WRW4 (Tocris, Minneapolis, MN) and [D-p-Cl-Phe 6 ,Leu 17 ]-VIP (Leu) (Tocris) (specific for FPR2 and VIPR, respectively) for 24 hours prior to VIP treatment and PA stimulation.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Infection

    Protein levels of FPR2 as detected by ELISA in PMN and macrophages after an in vitro stimulation assay. Peritoneal-derived PMN (A) and macrophages (B) from B6 mice were exposed to P. aeruginosa (PA) +/− the presence of VIP, VIP receptor antagonist and FPR2 antagonist. Data are reported as pg/mL ± SD (n = 6). *p<0.05 vs. CTRL; †p<0.05 vs. PA; ‡p<0.05 vs. PA+VIP expressed as mean ± SEM (n = 6 corneas/group).

    Journal: Prostaglandins & other lipid mediators

    Article Title: VIP Modulates the ALX/FPR2 Receptor Axis toward Inflammation Resolution in a Mouse Model of Bacterial Keratitis

    doi: 10.1016/j.prostaglandins.2018.12.001

    Figure Lengend Snippet: Protein levels of FPR2 as detected by ELISA in PMN and macrophages after an in vitro stimulation assay. Peritoneal-derived PMN (A) and macrophages (B) from B6 mice were exposed to P. aeruginosa (PA) +/− the presence of VIP, VIP receptor antagonist and FPR2 antagonist. Data are reported as pg/mL ± SD (n = 6). *p<0.05 vs. CTRL; †p<0.05 vs. PA; ‡p<0.05 vs. PA+VIP expressed as mean ± SEM (n = 6 corneas/group).

    Article Snippet: The cells were treated +/− select receptor antagonists, WRW4 (Tocris, Minneapolis, MN) and [D-p-Cl-Phe 6 ,Leu 17 ]-VIP (Leu) (Tocris) (specific for FPR2 and VIPR, respectively) for 24 hours prior to VIP treatment and PA stimulation.

    Techniques: Enzyme-linked Immunosorbent Assay, In Vitro, Derivative Assay

    A: Phase-contrast micrographs of 3-day-old cultures, illustrating that the growth rate of Hurler fibroblasts was considerably higher than those taken from normal children. Whereas the addition of insoluble elastin (arrows) to cultured normal fibroblasts did not affect their final density, addition of insoluble elastin (arrows) to cultures of Hurler fibroblasts substantially reduced their number in 3-day-old culture. B: Incorporation of 3H-thymidine also indicates higher growth of Hurler fibroblasts as compared with normal and Sanfilippo fibroblasts in 3-day-old cultures. These assays also illustrate that high proliferation of Hurler fibroblasts was significantly reduced in cultures treated with exogenous insoluble elastin (striated bars). C: Addition of DS, but not HS (both in concentrations of 400 μg/ml) significantly increased incorporation of 3H-thymidine to normal and Sanfilippo fibroblasts. Hurler fibroblasts cultured for 3 days in the presence of chondroitinase ABC (0.2 U/day) (Chnase ABC) decrease their 3H-thymidine incorporation. D: Pig intestinal SMCs (INTEST SMC), which do not produce elastin, incorporate more radioactive thymidine then the elastin-producing pig coronary artery SMCs (CA SMC). CA SMCs incubated for 3 days in the presence of 400 μg/ml of DS, up-regulate their incorporation of labeled thymidine, as compared with the untreated control and cells incubated with HS. In contrast, neither DS nor HS affected proliferation of pig intestinal SMCs. In all experiments, cells were plated with the same initial density 50,000 cells/well. Values of mean ± SD from 3 different experiments were statistically compared with untreated controls within the same cell type (*P < 0.002). Proliferation rates of different cell types tested were also assessed (**P < 0.05).

    Journal:

    Article Title: Impaired Elastogenesis in Hurler Disease

    doi:

    Figure Lengend Snippet: A: Phase-contrast micrographs of 3-day-old cultures, illustrating that the growth rate of Hurler fibroblasts was considerably higher than those taken from normal children. Whereas the addition of insoluble elastin (arrows) to cultured normal fibroblasts did not affect their final density, addition of insoluble elastin (arrows) to cultures of Hurler fibroblasts substantially reduced their number in 3-day-old culture. B: Incorporation of 3H-thymidine also indicates higher growth of Hurler fibroblasts as compared with normal and Sanfilippo fibroblasts in 3-day-old cultures. These assays also illustrate that high proliferation of Hurler fibroblasts was significantly reduced in cultures treated with exogenous insoluble elastin (striated bars). C: Addition of DS, but not HS (both in concentrations of 400 μg/ml) significantly increased incorporation of 3H-thymidine to normal and Sanfilippo fibroblasts. Hurler fibroblasts cultured for 3 days in the presence of chondroitinase ABC (0.2 U/day) (Chnase ABC) decrease their 3H-thymidine incorporation. D: Pig intestinal SMCs (INTEST SMC), which do not produce elastin, incorporate more radioactive thymidine then the elastin-producing pig coronary artery SMCs (CA SMC). CA SMCs incubated for 3 days in the presence of 400 μg/ml of DS, up-regulate their incorporation of labeled thymidine, as compared with the untreated control and cells incubated with HS. In contrast, neither DS nor HS affected proliferation of pig intestinal SMCs. In all experiments, cells were plated with the same initial density 50,000 cells/well. Values of mean ± SD from 3 different experiments were statistically compared with untreated controls within the same cell type (*P < 0.002). Proliferation rates of different cell types tested were also assessed (**P < 0.05).

    Article Snippet: D: Pig intestinal SMCs (INTEST SMC), which do not produce elastin, incorporate more radioactive thymidine then the elastin-producing pig coronary artery SMCs (CA SMC).

    Techniques: Cell Culture, Incubation, Labeling