picopure rna isolation kit Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher arcturus picopure rna isolation kit thermo fisher scientific waltham
    Comparison of <t>RNA</t> quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus <t>PicoPure</t> Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Arcturus Picopure Rna Isolation Kit Thermo Fisher Scientific Waltham, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arcturus picopure rna isolation kit thermo fisher scientific waltham/product/Thermo Fisher
    Average 99 stars, based on 4424 article reviews
    Price from $9.99 to $1999.99
    arcturus picopure rna isolation kit thermo fisher scientific waltham - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    84
    Thermo Fisher dy1857 picopure rna isolation kit thermo fisher scientific
    Comparison of <t>RNA</t> quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus <t>PicoPure</t> Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Dy1857 Picopure Rna Isolation Kit Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dy1857 picopure rna isolation kit thermo fisher scientific/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dy1857 picopure rna isolation kit thermo fisher scientific - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

    94
    Thermo Fisher rna isolation kit
    Comparison of <t>RNA</t> quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus <t>PicoPure</t> Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna isolation kit/product/Thermo Fisher
    Average 94 stars, based on 2701 article reviews
    Price from $9.99 to $1999.99
    rna isolation kit - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    91
    Thermo Fisher arturus picopure rna isolation kit
    Comparison of <t>RNA</t> quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus <t>PicoPure</t> Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p
    Arturus Picopure Rna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arturus picopure rna isolation kit/product/Thermo Fisher
    Average 91 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    arturus picopure rna isolation kit - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

    doi: 10.3389/fnmol.2017.00185

    Figure Lengend Snippet: Comparison of RNA quality using different LCM methods. (A) Graph comparing RNA quality (RIN) from LCM RNA samples captured using the MMI CellCut or Arcturus PixCell Instrument and extracted with either the Arcturus PicoPure Isolation kit or QIAGEN Micro RNeasy kit. An overall significant effect was found for both conditions using a two-way analyses of variance (ANOVA; CellCut vs. PixCell F (1,119) = 114.6; PicoPure vs. QIAGEN F (1,119) = 732.5). Although, it is important to note that two groups (Pixcell PicoPure and CellCut QIAGEN) were solely represented by one tissue type (see Experimental Summary in Table 1 ). There was also a significant interaction between the two conditions (Interaction F (1,119) = 9.177, p = 0.003). (B) The same data shown in A plotted by tissue type. Each tissue (Hippocampus, Midbrain and Liver) showed a significant increase in RIN with the QIAGEN kits vs. PicoPure kits using Sidak’s multiple comparisons post hoc test. All data were normally distributed (passed KS normality test) and had similar variances as tested by Brown-Forsythe test. (C,D) Representative Bioanalyzer gel (top) and electropherogram traces (bottom) from PixCell LCM RNA samples extracted using either the (C) Arcturus PicoPure Isolation kit or (D) QIAGEN Micro RNeasy kit. Note that these LCM samples were acquired simultaneously from different brain regions (CA1 vs. CA2) on the same sections from three mouse brains (#2, #4 or #6). Graphs are plotted min to max with a line at the mean. Numbers in parentheses indicate technical replicates. #### Overall group effect; **** post hoc result p

    Article Snippet: The lysates were loaded onto the spin column and total RNA purification was performed by following PicoPure® RNA isolation kit user guide (Thermo Fisher Scientific, Cat #KIT0204), including on-column DNase-treatment (QIAGEN, Cat#79254).

    Techniques: Laser Capture Microdissection, Isolation

    IF-LMF of TAL segments in the outer medulla. Representative images of IF-LMD of TAL segments from the outer medulla in a kidney section stained with Na + -K + -ATPase/DAPI/Phalloidin is depicted in A and B. Real-time PCR on RNA extracted from TAL cells demonstrates the increased expression of NKCC2 (specific marker) in TAL segments as compared to total kidney (* P

    Journal: Physiological Reports

    Article Title: Immunofluorescence laser micro-dissection of specific nephron segments in the mouse kidney allows targeted downstream proteomic analysis

    doi: 10.14814/phy2.12306

    Figure Lengend Snippet: IF-LMF of TAL segments in the outer medulla. Representative images of IF-LMD of TAL segments from the outer medulla in a kidney section stained with Na + -K + -ATPase/DAPI/Phalloidin is depicted in A and B. Real-time PCR on RNA extracted from TAL cells demonstrates the increased expression of NKCC2 (specific marker) in TAL segments as compared to total kidney (* P

    Article Snippet: RNA extraction and real-time PCR RNA was extracted using Pico-pure RNA kit (Life Technologies # 12204-01).

    Techniques: Laser Capture Microdissection, Staining, Real-time Polymerase Chain Reaction, Expressing, Marker

    Comparison of RNA extraction kits for microdissected samples. RNA quality for benign and PCa LCM samples are shown for all RNA extraction kits. RIN values are shown as the average of a duplicate experiment. RNeasy and miRNeasy consistently delivered high quality RNA from LCM samples ( a ). Median RIN values were compared between the groups, which were expected to be similar. However, RNeasy generated higher quality RNA than Picopure and RNAqueous (p = 0.01 and 0.08 respectively). Additionally, RNA quality between benign glands and PCa cells within the same patient was variable ( b )

    Journal: BMC Research Notes

    Article Title: Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue

    doi: 10.1186/s13104-015-1813-5

    Figure Lengend Snippet: Comparison of RNA extraction kits for microdissected samples. RNA quality for benign and PCa LCM samples are shown for all RNA extraction kits. RIN values are shown as the average of a duplicate experiment. RNeasy and miRNeasy consistently delivered high quality RNA from LCM samples ( a ). Median RIN values were compared between the groups, which were expected to be similar. However, RNeasy generated higher quality RNA than Picopure and RNAqueous (p = 0.01 and 0.08 respectively). Additionally, RNA quality between benign glands and PCa cells within the same patient was variable ( b )

    Article Snippet: RNA extraction and measurement The following RNA extraction kits were used to isolate total RNA from LCM acquired material: RNeasy® Micro (Qiagen, Germany), miRNeasy Mini (Qiagen, Germany), Arcturus® Picopure® RNA isolation kit (Arcturus, Applied Biosystems, USA.), mirVana™ miRNA isolation kit (Ambion, USA.) and RNAqueous® -Micro (Ambion, USA.).

    Techniques: RNA Extraction, Laser Capture Microdissection, Generated

    Expression analysis of genes identified in the papilla by LM–RNA-seq. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.

    Journal: Plant and Cell Physiology

    Article Title: Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

    doi: 10.1093/pcp/pct133

    Figure Lengend Snippet: Expression analysis of genes identified in the papilla by LM–RNA-seq. qRT-PCR was performed using the SYBR Green Real-Time PCR Master Mix with a Bio-Rad CFX96 Real-Time Detection System. cDNAs were prepared from total RNAs of stigma and leaf. Representation of the relative expression levels of the genes was normalized to the expression level of UBC21 . Quantitative validation of three replicates was calculated using the delta–delta threshold cycle relative quantification method.

    Article Snippet: RNA extraction, amplification and cDNA preparation Total RNAs of papilla cells collected by LM were extracted using the Pico-Pure RNA isolation kit (Applied Biosystems, Life Technologies).

    Techniques: Expressing, RNA Sequencing Assay, Quantitative RT-PCR, SYBR Green Assay, Real-time Polymerase Chain Reaction