phusion polymerase Search Results


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  • 99
    New England Biolabs phusion polymerase
    Agarose gel electrophoresis of extracted PCR products. Lane M, 1 kb DNA ladder; lane 1, cwp84 (predicted size, 2265 bp); lane 2, cspA (189 bp); lane 3, 5′ region of cwp66 (744 bp); lane 4, fbpA (1689 bp); lane 5, groEL (1461); lane 6, fliD (1497 bp); lane 7, acd (1650 bp); lane 8, fliC (780 bp); lane 9, 3′ region of cwp66 (900 bp); lane 10, sortaseB (579 bp); lane 11, cwp66 (1722 bp). All PCR reactions were carried out with <t>Phusion</t> DNA polymerase with buffer HF (lanes 2, 3, 5, 6, 7, 8, 9, 10) or GC buffer (for lanes 1, 4 and 11).
    Phusion Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/New England Biolabs
    Average 99 stars, based on 11131 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Thermo Fisher phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/Thermo Fisher
    Average 99 stars, based on 7207 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
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    93
    Fisher Scientific phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/Fisher Scientific
    Average 93 stars, based on 157 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
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    93
    ATUM phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by ATUM, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/ATUM
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
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    92
    PEQLAB phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by PEQLAB, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 6 article reviews
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    93
    Biozym phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by Biozym, used in various techniques. Bioz Stars score: 93/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/Biozym
    Average 93 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
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    92
    Illumina Inc phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/Illumina Inc
    Average 92 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
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    93
    TaKaRa phusion polymerase
    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) <t>Phusion;</t> (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.
    Phusion Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase/product/TaKaRa
    Average 93 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase - by Bioz Stars, 2020-07
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    99
    New England Biolabs phusionr high fidelity dna polymerase
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Phusionr High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 748 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusionr high fidelity dna polymerase/product/New England Biolabs
    Average 99 stars, based on 748 article reviews
    Price from $9.99 to $1999.99
    phusionr high fidelity dna polymerase - by Bioz Stars, 2020-07
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    99
    Thermo Fisher fidelity phusion polymerase
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Fidelity Phusion Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fidelity phusion polymerase/product/Thermo Fisher
    Average 99 stars, based on 73 article reviews
    Price from $9.99 to $1999.99
    fidelity phusion polymerase - by Bioz Stars, 2020-07
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    85
    Thermo Fisher phusion polymerase enzyme
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Phusion Polymerase Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 15 article reviews
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    phusion polymerase enzyme - by Bioz Stars, 2020-07
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    85
    Thermo Fisher phusion thermostable polymerase
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Phusion Thermostable Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    phusion thermostable polymerase - by Bioz Stars, 2020-07
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    93
    Promega phusion hotstart polymerase
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Phusion Hotstart Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion hotstart polymerase/product/Promega
    Average 93 stars, based on 5 article reviews
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    phusion hotstart polymerase - by Bioz Stars, 2020-07
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    86
    Thermo Fisher phusion polymerase protein
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Phusion Polymerase Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion polymerase protein/product/Thermo Fisher
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    phusion polymerase protein - by Bioz Stars, 2020-07
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    93
    Agilent technologies hercules phusion polymerase
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Hercules Phusion Polymerase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hercules phusion polymerase/product/Agilent technologies
    Average 93 stars, based on 5 article reviews
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    hercules phusion polymerase - by Bioz Stars, 2020-07
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    91
    Thermo Fisher blood phusion polymerase
    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using <t>Phusion</t> High-Fidelity <t>DNA</t> polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.
    Blood Phusion Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blood phusion polymerase/product/Thermo Fisher
    Average 91 stars, based on 6 article reviews
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    Image Search Results


    Agarose gel electrophoresis of extracted PCR products. Lane M, 1 kb DNA ladder; lane 1, cwp84 (predicted size, 2265 bp); lane 2, cspA (189 bp); lane 3, 5′ region of cwp66 (744 bp); lane 4, fbpA (1689 bp); lane 5, groEL (1461); lane 6, fliD (1497 bp); lane 7, acd (1650 bp); lane 8, fliC (780 bp); lane 9, 3′ region of cwp66 (900 bp); lane 10, sortaseB (579 bp); lane 11, cwp66 (1722 bp). All PCR reactions were carried out with Phusion DNA polymerase with buffer HF (lanes 2, 3, 5, 6, 7, 8, 9, 10) or GC buffer (for lanes 1, 4 and 11).

    Journal: Brazilian Journal of Microbiology

    Article Title: Isolation of recombinant antibodies directed against surface proteins of Clostridium difficile

    doi: 10.1016/j.bjm.2016.01.017

    Figure Lengend Snippet: Agarose gel electrophoresis of extracted PCR products. Lane M, 1 kb DNA ladder; lane 1, cwp84 (predicted size, 2265 bp); lane 2, cspA (189 bp); lane 3, 5′ region of cwp66 (744 bp); lane 4, fbpA (1689 bp); lane 5, groEL (1461); lane 6, fliD (1497 bp); lane 7, acd (1650 bp); lane 8, fliC (780 bp); lane 9, 3′ region of cwp66 (900 bp); lane 10, sortaseB (579 bp); lane 11, cwp66 (1722 bp). All PCR reactions were carried out with Phusion DNA polymerase with buffer HF (lanes 2, 3, 5, 6, 7, 8, 9, 10) or GC buffer (for lanes 1, 4 and 11).

    Article Snippet: PCR Amplification of candidate sequences was performed in 50 μL reactions, each containing 2 μL of genomic DNA from C. difficile strain 630, 0.5 μM each primer , 200 μM dNTPs and 0.5 μL of Phusion polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction

    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) Phusion; (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.

    Journal: BMC Genomics

    Article Title: Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes

    doi: 10.1186/1471-2164-13-1

    Figure Lengend Snippet: Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) Phusion; (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.

    Article Snippet: Standard Illumina PCR (50 μl) with Phusion polymerase (Thermo) contained 1× Phusion DNA polymerase master mix and 0.4 μM of each primer pair and was amplified with thermocycling conditions of 1 min at 98°C for the initial denaturation, followed by 12 cycles of [10 s at 98°C, 30 s at 65°C, 30 s at 72°C] and a final extension for 5 min at 72°C.

    Techniques: Polymerase Chain Reaction, Amplification, Positive Control

    Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) Phusion; (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.

    Journal: BMC Genomics

    Article Title: Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes

    doi: 10.1186/1471-2164-13-1

    Figure Lengend Snippet: Screening for tolerance to an AT-rich template using conventional PCR amplification . Top panel: PCR amplification of a 540 bp locus (Pf3D7_11:1294982-1295521) with a relatively balanced (70% AT) base composition (positive control) in the presence or absence of TMAC. Bottom panel: PCR amplification of a 1217 bp locus (Pf3D7_01:55900-57116) with extreme AT content (84%) in the presence or absence of TMAC. M, 100 bp DNA ladder (NEB); (1) PWO master; (2) PWO master + TMAC; (3) PfuULTRA; (4) PfuULTRA + TMAC; (5) Kapa HiFi; (6) Kapa HiFi + TMAC; (7) AccuPrime Taq HiFi; (8) AccuPrime Taq HiFi + TMAC; (9) AccuPrime pfx SuperMix; (10) Phusion; (11) Phusion +TMAC; (12) Platinum HiFi; (13) Platinum HiFi + TMAC; (14) Platinum pfx; (15) Platinum pfx + TMAC, (16) Ex Taq; (17) Ex Taq + TMAC; (18) Kapa2G Robust; (19) Kapa2G Robust + TMAC.

    Article Snippet: PCR amplification of Illumina genomic libraries Although Phusion polymerase is the standard enzyme for Illumina sequencing library amplification, it produces libraries with poor representation of loci with extreme base composition particularly the AT-rich regions [ ].

    Techniques: Polymerase Chain Reaction, Amplification, Positive Control

    RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.

    Journal: PLoS ONE

    Article Title: Variations of five eIF4E genes across cassava accessions exhibiting tolerant and susceptible responses to cassava brown streak disease

    doi: 10.1371/journal.pone.0181998

    Figure Lengend Snippet: RT-PCR amplification of five cassava eIF4E ORFs with gene-specific primers. Total RNA was extracted from TMS60444 cassava line. The first-strand cDNA was synthesized using SuperScript ™ III Reverse Transcriptase (Invitrogen) and PCR was performed using Phusion High-Fidelity DNA polymerase (New England Biolabs) and primers indicated in Table 1 . Lane 1: DNA marker; Lane 2: 016601 CDS amplified with primers 016601F and 016601R; Lane 3: 016620 CDS amplified with primers 016620F and 016620R; Lane 4: 015501 CDS amplified with 015501F and 015501R, Lane 5: 013223 CDS amplified with primers 013223lF and 013223R, Lane 6, 013223 CDS amplified with primers 013223sF and 013223R; Lane 7: 013732 CDS amplified with 013732lF and 013732R; Lane 8: 013732 CDS amplified with 013732sF and 013732R; and Lane 9: negative water control.

    Article Snippet: PCR was performed in a 20 μl reaction volume containing 10 unit Phusion DNA polymerase (NEB, Ipswich, MA), 1 μl of 1:5 diluted cDNA template, 1X Phusion PCR buffer, 5 μM each of upstream and downstream primers, and 250 nM dNTP with the following cycling condition: 98°C for 1 minute; 35 cycles of 98°C for 15 seconds, 56°C for 15 seconds, and 72°C for 45 seconds; and finally 72°C for 5 minutes.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Synthesized, Polymerase Chain Reaction, Marker