phusion dna polymerase Thermo Fisher Search Results


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  • 99
    Thermo Fisher phusion dna polymerase
    ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by <t>Phusion</t> <t>DNA</t> polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.
    Phusion Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10263 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phusion dna polymerase - by Bioz Stars, 2020-04
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    90
    Thermo Fisher elongase dna polymerase
    ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by <t>Phusion</t> <t>DNA</t> polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.
    Elongase Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher phusion hotstart dna polymerase
    ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by <t>Phusion</t> <t>DNA</t> polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.
    Phusion Hotstart Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion hotstart dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    phusion hotstart dna polymerase - by Bioz Stars, 2020-04
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    99
    Thermo Fisher phusion hot start dna polymerase
    ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by <t>Phusion</t> <t>DNA</t> polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.
    Phusion Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion hot start dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 581 article reviews
    Price from $9.99 to $1999.99
    phusion hot start dna polymerase - by Bioz Stars, 2020-04
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    95
    Thermo Fisher high fidelity phusion flash dna polymerase
    ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by <t>Phusion</t> <t>DNA</t> polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.
    High Fidelity Phusion Flash Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher phusion high fidelity hot start dna polymerase
    LINE-1 insertion in intron 2 of AHR in Irish Wolfhounds. Southern blot analysis (A) with HindIII (lanes 1–4) and PstI (lanes 5–8) digested genomic <t>DNA.</t> Digestion with HindIII resulted in an increase in size of about 2000 bp in Irish wolfhounds (lanes 1 and 2) compared to healthy beagles (lanes 3 and 4). Using PstI as restriction enzyme results in a small increase in size of approximately 400 bp in Irish wolfhounds (lanes 5 and 6) compared to healthy beagles (lanes 7 and 8). The observed size differences are smaller than the actual rearrangement due to the presence of restriction enzyme sites in the rearranged DNA fragment. Gel electrophoresis of PCR products (B) with forward primer in exon 2 and reverse primer downstream intron 2. The use of <t>Phusion</t> ™ Hot Start High-Fidelity DNA Polymerase in combination with GC-buffer was required to amplify this fragment. An increase in intron size was detected in all used gDNA samples of Irish wolfhounds. Lane 1∶1 kb DNA ladder (Promega, Leiden, the Netherlands); 2: normal control; 3–5: genomic DNA of Irish wolfhounds; 6: negative control; 7∶100 bp DNA ladder (Promega). A 6298 bp long LINE-1 insertion named L1-Y_CF (C) is located 63 bp downstream of exon2. It contains a 13 bp long duplication site specific for LINE-1 insertions. In both open reading frames of this retrotransposon deletions were detected causing frame shifts and premature stop codons. Restriction sites were found for HindIII at 1908 bp and for PstI at 447 bp.
    Phusion High Fidelity Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion high fidelity hot start dna polymerase/product/Thermo Fisher
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    99
    Thermo Fisher dna polymerase
    LINE-1 insertion in intron 2 of AHR in Irish Wolfhounds. Southern blot analysis (A) with HindIII (lanes 1–4) and PstI (lanes 5–8) digested genomic <t>DNA.</t> Digestion with HindIII resulted in an increase in size of about 2000 bp in Irish wolfhounds (lanes 1 and 2) compared to healthy beagles (lanes 3 and 4). Using PstI as restriction enzyme results in a small increase in size of approximately 400 bp in Irish wolfhounds (lanes 5 and 6) compared to healthy beagles (lanes 7 and 8). The observed size differences are smaller than the actual rearrangement due to the presence of restriction enzyme sites in the rearranged DNA fragment. Gel electrophoresis of PCR products (B) with forward primer in exon 2 and reverse primer downstream intron 2. The use of <t>Phusion</t> ™ Hot Start High-Fidelity DNA Polymerase in combination with GC-buffer was required to amplify this fragment. An increase in intron size was detected in all used gDNA samples of Irish wolfhounds. Lane 1∶1 kb DNA ladder (Promega, Leiden, the Netherlands); 2: normal control; 3–5: genomic DNA of Irish wolfhounds; 6: negative control; 7∶100 bp DNA ladder (Promega). A 6298 bp long LINE-1 insertion named L1-Y_CF (C) is located 63 bp downstream of exon2. It contains a 13 bp long duplication site specific for LINE-1 insertions. In both open reading frames of this retrotransposon deletions were detected causing frame shifts and premature stop codons. Restriction sites were found for HindIII at 1908 bp and for PstI at 447 bp.
    Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-04
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    93
    Thermo Fisher high fidelity phushion dna polymerase
    LINE-1 insertion in intron 2 of AHR in Irish Wolfhounds. Southern blot analysis (A) with HindIII (lanes 1–4) and PstI (lanes 5–8) digested genomic <t>DNA.</t> Digestion with HindIII resulted in an increase in size of about 2000 bp in Irish wolfhounds (lanes 1 and 2) compared to healthy beagles (lanes 3 and 4). Using PstI as restriction enzyme results in a small increase in size of approximately 400 bp in Irish wolfhounds (lanes 5 and 6) compared to healthy beagles (lanes 7 and 8). The observed size differences are smaller than the actual rearrangement due to the presence of restriction enzyme sites in the rearranged DNA fragment. Gel electrophoresis of PCR products (B) with forward primer in exon 2 and reverse primer downstream intron 2. The use of <t>Phusion</t> ™ Hot Start High-Fidelity DNA Polymerase in combination with GC-buffer was required to amplify this fragment. An increase in intron size was detected in all used gDNA samples of Irish wolfhounds. Lane 1∶1 kb DNA ladder (Promega, Leiden, the Netherlands); 2: normal control; 3–5: genomic DNA of Irish wolfhounds; 6: negative control; 7∶100 bp DNA ladder (Promega). A 6298 bp long LINE-1 insertion named L1-Y_CF (C) is located 63 bp downstream of exon2. It contains a 13 bp long duplication site specific for LINE-1 insertions. In both open reading frames of this retrotransposon deletions were detected causing frame shifts and premature stop codons. Restriction sites were found for HindIII at 1908 bp and for PstI at 447 bp.
    High Fidelity Phushion Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by Phusion DNA polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.

    Journal: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]

    Article Title: Improved Protocols for Illumina Sequencing

    doi: 10.1002/0471142905.hg1802s62

    Figure Lengend Snippet: ( A ) Template hybridization, extension, and denaturation on the flowcell surface. Templates are prepared so as to possess tails that are complementary to primers on the flowcell surface. This allows one end of a template strand to hybridize to a flowcell primer. Flowcell primers are extended by Phusion DNA polymerase (Thermo Scientific), resulting in a reverse complementary copy of the original template strand, which is covalently attached to the flowcell surface. The original template strand is then removed by flushing 0.1 M NaOH though the flowcell. ( B ) Cluster amplification. The free end of the tethered reverse complementary copy of the original template strand can anneal to the other type of flowcell primer, forming a bridge. The flowcell primer is extended by Bst polymerase, in an isothermal reaction, which generates a double-stranded product. Formamide is used to denature these strands, which can then anneal to other primers on the flowcell surface, which extend in the next cycle. In this way, repeated cycles of extension and denaturation result in a cluster of strands, all of which are derived from a single template strand.

    Article Snippet: Flowcell primers are extended, by Phusion DNA polymerase (Thermo Scientific), generating a reverse complementary copy of the original template strand that is tethered to the flowcell surface.

    Techniques: Hybridization, Amplification, Derivative Assay

    DLC1 -i4 is silenced by DNA methylation in carcinoma cell lines and demethylation by pharmacological or genetic approach could induce DLC1 -i4 reexpression. ( a ) Schematic diagram of the location of 59 CpG dinucleotides along an 800 bp fragment encompassing

    Journal: Oncogene

    Article Title: A novel isoform of the 8p22 tumor suppressor gene DLC1 suppresses tumor growth and is frequently silenced in multiple common tumors

    doi: 10.1038/onc.2010.576

    Figure Lengend Snippet: DLC1 -i4 is silenced by DNA methylation in carcinoma cell lines and demethylation by pharmacological or genetic approach could induce DLC1 -i4 reexpression. ( a ) Schematic diagram of the location of 59 CpG dinucleotides along an 800 bp fragment encompassing

    Article Snippet: From normal placenta DNA (Sigma-Aldrich Corporation, St Louis, MO, USA), three regions of the putative DLC1 -i4 and -i1 promoter were PCR amplified using a high-fidelity DNA polymerase, Phusion (Finnzymes, Espoo, Finland) and ligated to the promoter-less pGL2-Enhancer vector (Promega, Madison, WI, USA) to create pGL2-DLC1i4-PF1/R(−1840/+140), pGL2-DLC1i4-PF2/R(−960/+140), pGL2-DLC1i4-PF3/R(−480/+140); pGL2-DLC1i1-F1/R(−1272/+286), pGL2-DLC1i1-F2/R(−656/+286) and pGL2-DLC1i1-F3/R(−376/+286).

    Techniques: DNA Methylation Assay

    Proportion of correct reads for the three genetic systems (simple: a single allele per individual, squares ; medium: two alleles, circles ; and complex: multiple alleles, triangles ) using standard PCR conditions ( open ) and modified PCR conditions to reduce chimera formation ( gray ). The size of the shape is indicative of the number of reads (see legend). All enzymes yielded at least 50% correct reads in the simplest system, mitochondrial DNA (Test 1; open squares). Some enzymes only worked for a given set of conditions (cycling conditions/genetic system). A group of enzymes consisting of Phusion, Gold and FastStart yielded a high proportion of correct reads cosistently accross all conditions. Others, such as Roche Taq, HotStar and Biotaq, yielded a low percent of correct reads for the more complex systems (MHC class I and MHC class II). Abbreviations as defined in Table 1 .

    Journal: Scientific Reports

    Article Title: Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results

    doi: 10.1038/srep08056

    Figure Lengend Snippet: Proportion of correct reads for the three genetic systems (simple: a single allele per individual, squares ; medium: two alleles, circles ; and complex: multiple alleles, triangles ) using standard PCR conditions ( open ) and modified PCR conditions to reduce chimera formation ( gray ). The size of the shape is indicative of the number of reads (see legend). All enzymes yielded at least 50% correct reads in the simplest system, mitochondrial DNA (Test 1; open squares). Some enzymes only worked for a given set of conditions (cycling conditions/genetic system). A group of enzymes consisting of Phusion, Gold and FastStart yielded a high proportion of correct reads cosistently accross all conditions. Others, such as Roche Taq, HotStar and Biotaq, yielded a low percent of correct reads for the more complex systems (MHC class I and MHC class II). Abbreviations as defined in Table 1 .

    Article Snippet: Taq polymerase A range of 13 high fidelity, regular, economy and premium Taq polymerase enzymes were selected: Biotaq® (Bioline, London, UK), FastStart® High Fidelity PCR System (Roche, Mannheim, Germany), AmpliTaq Gold® (Applied Biosystems, Warrington, UK), HotStarTaq® DNA Polymerase (Qiagen, Hilden, Gernamy), Phusion® High Fidelity DNA Polymerase (Finnzymes, Espoo, Finland), Taq DNA Polymerase (Roche, Maylan, France), i-MaxTM II DNA Polymerase (iNtRON Biotechnology, Seongnam, Korea), KAPA HiFi™ (Kapa Biosystems, Boston, USA), OneTaq ™ DNA Polymerase (New England Biolabs, Hitchin, UK), Vent® DNA Polymerase (New England Biolabs, Hitchin, UK), Deep Vent® DNA Polymerase (New England Biolabs, Hitchin, UK), Pwo® DNA Polymerase (Roche, Maylan, France) and Velocity DNA Polymerase (Bioline, London, UK) (abbreviated names in ).

    Techniques: Polymerase Chain Reaction, Modification

    LINE-1 insertion in intron 2 of AHR in Irish Wolfhounds. Southern blot analysis (A) with HindIII (lanes 1–4) and PstI (lanes 5–8) digested genomic DNA. Digestion with HindIII resulted in an increase in size of about 2000 bp in Irish wolfhounds (lanes 1 and 2) compared to healthy beagles (lanes 3 and 4). Using PstI as restriction enzyme results in a small increase in size of approximately 400 bp in Irish wolfhounds (lanes 5 and 6) compared to healthy beagles (lanes 7 and 8). The observed size differences are smaller than the actual rearrangement due to the presence of restriction enzyme sites in the rearranged DNA fragment. Gel electrophoresis of PCR products (B) with forward primer in exon 2 and reverse primer downstream intron 2. The use of Phusion ™ Hot Start High-Fidelity DNA Polymerase in combination with GC-buffer was required to amplify this fragment. An increase in intron size was detected in all used gDNA samples of Irish wolfhounds. Lane 1∶1 kb DNA ladder (Promega, Leiden, the Netherlands); 2: normal control; 3–5: genomic DNA of Irish wolfhounds; 6: negative control; 7∶100 bp DNA ladder (Promega). A 6298 bp long LINE-1 insertion named L1-Y_CF (C) is located 63 bp downstream of exon2. It contains a 13 bp long duplication site specific for LINE-1 insertions. In both open reading frames of this retrotransposon deletions were detected causing frame shifts and premature stop codons. Restriction sites were found for HindIII at 1908 bp and for PstI at 447 bp.

    Journal: PLoS ONE

    Article Title: Altered Subcellular Localization of Heat Shock Protein 90 Is Associated with Impaired Expression of the Aryl Hydrocarbon Receptor Pathway in Dogs

    doi: 10.1371/journal.pone.0057973

    Figure Lengend Snippet: LINE-1 insertion in intron 2 of AHR in Irish Wolfhounds. Southern blot analysis (A) with HindIII (lanes 1–4) and PstI (lanes 5–8) digested genomic DNA. Digestion with HindIII resulted in an increase in size of about 2000 bp in Irish wolfhounds (lanes 1 and 2) compared to healthy beagles (lanes 3 and 4). Using PstI as restriction enzyme results in a small increase in size of approximately 400 bp in Irish wolfhounds (lanes 5 and 6) compared to healthy beagles (lanes 7 and 8). The observed size differences are smaller than the actual rearrangement due to the presence of restriction enzyme sites in the rearranged DNA fragment. Gel electrophoresis of PCR products (B) with forward primer in exon 2 and reverse primer downstream intron 2. The use of Phusion ™ Hot Start High-Fidelity DNA Polymerase in combination with GC-buffer was required to amplify this fragment. An increase in intron size was detected in all used gDNA samples of Irish wolfhounds. Lane 1∶1 kb DNA ladder (Promega, Leiden, the Netherlands); 2: normal control; 3–5: genomic DNA of Irish wolfhounds; 6: negative control; 7∶100 bp DNA ladder (Promega). A 6298 bp long LINE-1 insertion named L1-Y_CF (C) is located 63 bp downstream of exon2. It contains a 13 bp long duplication site specific for LINE-1 insertions. In both open reading frames of this retrotransposon deletions were detected causing frame shifts and premature stop codons. Restriction sites were found for HindIII at 1908 bp and for PstI at 447 bp.

    Article Snippet: The thermal cycling protocol consisted of a 5-min denaturation step at 95°C, 35 cycles of 30 s at 95°C, 30 s at annealing temperature, 30 s at 72°C, and a final elongation step at 72°C for 10 min. For amplification of fragments exceeding 2000 bp, Phusion ™ Hot Start High-Fidelity DNA Polymerase (Thermo Scientific, Lafayette, US) was used.

    Techniques: Southern Blot, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control