phosphate-buffered saline Search Results


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    Boster†s Phosphate Buffered Saline PBS Powder is a dehydrated raw material reagent intended to be used in the making of prepared media products by reconstitution in distilled water
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    pbs  (Lonza)
    97
    Lonza pbs
    Pbs, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 1233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pbs
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    Millipore pbs
    Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    LI-COR odyssey blocking buffer
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    Thermo Fisher d pbs
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    Agilent technologies pbs
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    Millipore dulbecco s phosphate buffered saline
    Dulbecco S Phosphate Buffered Saline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific pbs
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    Thermo Fisher bsa pbs
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    pbs  (Abcam)
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    Thermo Fisher dulbecco s phosphate buffered saline dpbs
    Dulbecco S Phosphate Buffered Saline Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2942 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 1x pbs
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    GE Healthcare pbs
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    Thermo Fisher pbs phosphate buffered saline 10x ph 7 4
    Pbs Phosphate Buffered Saline 10x Ph 7 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad phosphate buffered saline
    Phosphate Buffered Saline, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson pbs
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    corning life sciences pbs
    Inhibition of syncytium formation by sera from convalescent donors. Vero cells were infected with pre-titrated amounts of <t>rVSV-SARS-CoV-2.</t> At 2 h post-infection, cells were washed with <t>PBS</t> to remove residual virus and then exposed to the indicated dilutions of each convalescent serum. Cells were fixed, their nuclei counterstained, and they were imaged for syncytium formation by eGFP expression at 16 h post-infection. Representative images from one of two independent experiments are shown.
    Pbs, supplied by corning life sciences, used in various techniques. Bioz Stars score: 92/100, based on 413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime pbs
    Inhibition of syncytium formation by sera from convalescent donors. Vero cells were infected with pre-titrated amounts of <t>rVSV-SARS-CoV-2.</t> At 2 h post-infection, cells were washed with <t>PBS</t> to remove residual virus and then exposed to the indicated dilutions of each convalescent serum. Cells were fixed, their nuclei counterstained, and they were imaged for syncytium formation by eGFP expression at 16 h post-infection. Representative images from one of two independent experiments are shown.
    Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 95/100, based on 926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA pbs
    <t>SPS-neutralization</t> in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in <t>PBS</t> or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p
    Pbs, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 1284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore phosphate buffer saline pbs
    <t>SPS-neutralization</t> in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in <t>PBS</t> or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p
    Phosphate Buffer Saline Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2095 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    11 34 g makes 1 L reconstituted buffer
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    Image Search Results


    Inhibition of syncytium formation by sera from convalescent donors. Vero cells were infected with pre-titrated amounts of rVSV-SARS-CoV-2. At 2 h post-infection, cells were washed with PBS to remove residual virus and then exposed to the indicated dilutions of each convalescent serum. Cells were fixed, their nuclei counterstained, and they were imaged for syncytium formation by eGFP expression at 16 h post-infection. Representative images from one of two independent experiments are shown.

    Journal: bioRxiv

    Article Title: A replication-competent vesicular stomatitis virus for studies of SARS-CoV-2 spike-mediated cell entry and its inhibition

    doi: 10.1101/2020.05.20.105247

    Figure Lengend Snippet: Inhibition of syncytium formation by sera from convalescent donors. Vero cells were infected with pre-titrated amounts of rVSV-SARS-CoV-2. At 2 h post-infection, cells were washed with PBS to remove residual virus and then exposed to the indicated dilutions of each convalescent serum. Cells were fixed, their nuclei counterstained, and they were imaged for syncytium formation by eGFP expression at 16 h post-infection. Representative images from one of two independent experiments are shown.

    Article Snippet: S-mediated antibody depletion assayHigh-protein binding 96-well ELISA plates (Corning) coated with PBS alone or with 2 µg/ml of SARS-CoV-2 S protein in PBS overnight at 4°C were blocked for 1 hour with 3% nonfat dry milk (Biorad) in PBS.

    Techniques: Inhibition, Infection, Expressing

    SPS-neutralization in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in PBS or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Journal: BMC Infectious Diseases

    Article Title: SPS-neutralization in tissue samples for efficacy testing of antimicrobial peptides

    doi: 10.1186/s12879-019-4700-1

    Figure Lengend Snippet: SPS-neutralization in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in PBS or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Article Snippet: SPS-neutralization of antimicrobial activity Ten μL of PBS or one of the antimicrobial agents: 1% (wt/v) SAAP-148 in PBS, 1% (wt/v) pexiganan in PBS, 1% (wt/wt) SSD, 0.5% (v/v) chlorhexidine in 70% alcohol, 2% (wt/wt) Bactroban or 2% (wt/wt) Fucidin were added to polypropylene vials containing 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS (Fig. ) in PBS (Merck, KGaA, Darmstadt, Germany).

    Techniques: Neutralization

    Effect of SPS on the antimicrobial activity of various antimicrobial agents. Mixtures of 10 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS and 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS in PBS were prepared. Ninety μL of LUH14616 with a final concentration of 10 5 CFU/mL were added to these mixtures to determine the antimicrobial activity after 30 min incubation at 37 °C and 5% CO 2 . The means and standard deviations (SD) of three independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Journal: BMC Infectious Diseases

    Article Title: SPS-neutralization in tissue samples for efficacy testing of antimicrobial peptides

    doi: 10.1186/s12879-019-4700-1

    Figure Lengend Snippet: Effect of SPS on the antimicrobial activity of various antimicrobial agents. Mixtures of 10 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS and 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS in PBS were prepared. Ninety μL of LUH14616 with a final concentration of 10 5 CFU/mL were added to these mixtures to determine the antimicrobial activity after 30 min incubation at 37 °C and 5% CO 2 . The means and standard deviations (SD) of three independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Article Snippet: SPS-neutralization of antimicrobial activity Ten μL of PBS or one of the antimicrobial agents: 1% (wt/v) SAAP-148 in PBS, 1% (wt/v) pexiganan in PBS, 1% (wt/wt) SSD, 0.5% (v/v) chlorhexidine in 70% alcohol, 2% (wt/wt) Bactroban or 2% (wt/wt) Fucidin were added to polypropylene vials containing 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS (Fig. ) in PBS (Merck, KGaA, Darmstadt, Germany).

    Techniques: Activity Assay, Concentration Assay, Incubation

    SPS-neutralization of residual activity of various antimicrobial agents. Excision wound models were inoculated with 10 5 CFU/mL LUH14616 for 1 h and exposed to 20 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS for 1 h. Subsequently, the models were homogenized in 1 mL of PBS with or without 0.05% (wt/v) SPS. The means and SD of at least eight independent experiments performed in triplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Journal: BMC Infectious Diseases

    Article Title: SPS-neutralization in tissue samples for efficacy testing of antimicrobial peptides

    doi: 10.1186/s12879-019-4700-1

    Figure Lengend Snippet: SPS-neutralization of residual activity of various antimicrobial agents. Excision wound models were inoculated with 10 5 CFU/mL LUH14616 for 1 h and exposed to 20 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS for 1 h. Subsequently, the models were homogenized in 1 mL of PBS with or without 0.05% (wt/v) SPS. The means and SD of at least eight independent experiments performed in triplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Article Snippet: SPS-neutralization of antimicrobial activity Ten μL of PBS or one of the antimicrobial agents: 1% (wt/v) SAAP-148 in PBS, 1% (wt/v) pexiganan in PBS, 1% (wt/wt) SSD, 0.5% (v/v) chlorhexidine in 70% alcohol, 2% (wt/wt) Bactroban or 2% (wt/wt) Fucidin were added to polypropylene vials containing 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS (Fig. ) in PBS (Merck, KGaA, Darmstadt, Germany).

    Techniques: Neutralization, Activity Assay