New England Biolabs
phi29 dna polymerase Phi29 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 839 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/phi29 dna polymerase/product/New England Biolabs Average 99 stars, based on 839 article reviews Price from $9.99 to $1999.99
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2021-01
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Thermo Fisher
phi29 dna polymerase ![]() Phi29 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/phi29 dna polymerase/product/Thermo Fisher Average 99 stars, based on 459 article reviews Price from $9.99 to $1999.99
phi29 dna polymerase - by Bioz Stars,
2021-01
99/100 stars
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Thermo Fisher
reaction buffer for phi29 dna polymerase 10x ![]() Reaction Buffer For Phi29 Dna Polymerase 10x, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/reaction buffer for phi29 dna polymerase 10x/product/Thermo Fisher Average 90 stars, based on 38 article reviews Price from $9.99 to $1999.99
reaction buffer for phi29 dna polymerase 10x - by Bioz Stars,
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Thermo Fisher
phi29 dna polymerase reaction buffer ![]() Phi29 Dna Polymerase Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/phi29 dna polymerase reaction buffer/product/Thermo Fisher Average 88 stars, based on 25 article reviews Price from $9.99 to $1999.99
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Thermo Fisher
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Image Search Results

Journal: Scientific Reports
Article Title: Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
doi: 10.1038/s41598-017-00755-y
Figure Lengend Snippet: Detection of cells expressing BCR-ABL1 with PLA-flow. The assay employs a pair of oligonucleotide-conjugated antibodies (PLA probes) with affinity for the BCR and ABL1 domains of the fusion protein in fixed and permeabilized cells ( A ). The oligonucleotides on PLA probes remaining in close proximity after washes serve as templates for hybridization of two additional DNA oligonucleotides, guiding their ligation into DNA circles ( B ). After ligation, the DNA circles are locally amplified by rolling circle amplification (RCA) to generate RCA products that are visualized and detected by addition of complementary fluorophore-labeled oligonucleotides ( C ), followed by detection of labeled cells via flow cytometry.
Article Snippet: 0.5 U/µl phi-29 DNA polymerase (Fermentas) was mixed with
Techniques: Expressing, Proximity Ligation Assay, Flow Cytometry, Hybridization, Ligation, Amplification, Labeling, Cytometry

Journal: RNA
Article Title: Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3?-end
doi: 10.1261/rna.2068510
Figure Lengend Snippet: Target RNA conversion into a primer for RCA, the impact of E. coli RNase III. The reactions were carried out under the conditions described in Materials and Methods. The 5′-end-labeled ( A ) RNA2-PP1 (panel 1 ), ( B ) RNA2-PP2 (panel 1 ), and RNA1-PP1 (panel 2 ) hybrids were incubated with Phi29 DNA polymerase in the absence or presence of dNTP. ( A , panel 2 ) The experiments with E. coli RNase III were performed using RNA2-PP1 duplex. The RNA degradation and RCA products were analyzed by electrophoresis through denaturing 8% polyacrylamide gels. Different RNA-PP duplexes are shown above the gels. The radioactive label is indicated by the star. The contents of samples are shown above the gel lines. Reaction products are labeled as “ a ” for the 5′-end-labeled RNA1 hydrolysis product serving as an RNA primer for RCA and “ b ” for the 5′-end-labeled RCA product. ( C ) The reaction scheme represents the conversions of target RNA.
Article Snippet: BSA, DEPC-treated water, DNase I,
Techniques: Labeling, Incubation, Electrophoresis