phi29 dna polymerase Search Results


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  • 99
    Qiagen repli g kit
    Repli G Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phi 29 dna polymerase
    Phi 29 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs phi 29 dna polymerase
    Phi 29 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare phi29 dna polymerase
    Phi29 Dna Polymerase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Olink Bioscience phi29 dna polymerase
    Phi29 Dna Polymerase, supplied by Olink Bioscience, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phi29 dna polymerase
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Phi29 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Epicentre Biotechnologies repliphi phi29 dna polymerase
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Repliphi Phi29 Dna Polymerase, supplied by Epicentre Biotechnologies, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phi29 dna polymerase reaction buffer
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Phi29 Dna Polymerase Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kanto Chemical dna free phi29 dna polymerase
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Dna Free Phi29 Dna Polymerase, supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare genomiphi v2 dna polymerase kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Genomiphi V2 Dna Polymerase Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    GE Healthcare phi29 amplified dna
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Phi29 Amplified Dna, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare illustra genomiphi hy kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Illustra Genomiphi Hy Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 84/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare genomiphi kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Genomiphi Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare genomiphi dna amplification kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Genomiphi Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 584 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare illustra templiphi kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Illustra Templiphi Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare illustra genomiphi v2 dna amplification kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Illustra Genomiphi V2 Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare genomiphi v2 kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Genomiphi V2 Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs deoxynucleotide solution set
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Deoxynucleotide Solution Set, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen repli g mini kit
    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic <t>DNA</t> was isolated from U2OS cells and processed to detect <t>Phi29-dependent</t> telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p
    Repli G Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Phi29 DNA polymerase is a recombinant protein purified from E coli cloned the gene encoding the DNA polymerase from Phi29 phage Phi29 DNA polymerase is the replicative polymerase from the
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    Image Search Results


    Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic DNA was isolated from U2OS cells and processed to detect Phi29-dependent telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p

    Journal: Molecular Cell

    Article Title: SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance

    doi: 10.1016/j.molcel.2019.07.010

    Figure Lengend Snippet: Loss of SLX4IP in ALT-Positive Cells Increases ALT-Related Phenotypes (A) Genomic DNA was isolated from U2OS cells and processed to detect Phi29-dependent telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ , which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p

    Article Snippet: Primer extension was carried out with 7.5 U of Phi29 DNA polymerase (Thermo Scientific) at 30°C for 12 hours.

    Techniques: Isolation, Amplification, Southern Blot, Labeling, Autoradiography

    SLX4 Depletion Further Augments the Increase in ALT-Related Phenotypes in SLX4IP −/− Cells (A) U2OS cells were transfected with the indicated siRNAs. Their genomic DNA was then processed to detect Phi29-dependent telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ siCTRL, which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p

    Journal: Molecular Cell

    Article Title: SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance

    doi: 10.1016/j.molcel.2019.07.010

    Figure Lengend Snippet: SLX4 Depletion Further Augments the Increase in ALT-Related Phenotypes in SLX4IP −/− Cells (A) U2OS cells were transfected with the indicated siRNAs. Their genomic DNA was then processed to detect Phi29-dependent telomere circles. The Phi29 amplification products were detected by Southern blotting using a γ[ 32 P]-labeled telomeric (TTAGGG) probe. (B) Quantification of (A). The extent of [ 32 P] incorporation was quantified from the autoradiograph and normalized to SLX4IP +/+ siCTRL, which was arbitrarily assigned a value of 1. Data are represented as mean ± SD; n = 3; ∗ p

    Article Snippet: Primer extension was carried out with 7.5 U of Phi29 DNA polymerase (Thermo Scientific) at 30°C for 12 hours.

    Techniques: Transfection, Amplification, Southern Blot, Labeling, Autoradiography