Journal: Radiology and Oncology

Article Title: Mild hyperthermia influence on Herceptin ® properties

doi: 10.2478/raon-2014-0045

Figure Lengend Snippet: Degradation and Dephosphorylation of HER-2 receptors by Herceptin ® . 4×10 5 BT-474 cells were seeded in a 6-well plate and treated with Herceptin ® (50 μg/mL) for 6 days. Immunoblots of protein lysates were analyzed for total and phosphorylated HER-2 receptors. Representative immunoblots of one experiment out of three independent experiments are shown. The number at the bottom of each lane indicates the relative fold change versus the control after normalization with the β-ACTIN signal. Data expressed as mean ± SD calculated from three independent experiments. Statistical analysis was performed using the non-parametric Mann-Whitney test. Significance was defined as p < 0.05 (NS, non-significant; * p < 0.05 compared to the positive condition).

Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal secondary antibody anti-human IgG1-Fc (Ab50473), rabbit polyclonal anti-HER 2 (ab2428), anti-pHER-2 (ab47263), anti-ACTIN (ab1801) antibodies and horseradish peroxidase (HRP)-conjugated goat polyclonal secondary anti-Rabbit IgG Fc (ab97200) were obtained from Abcam ® (Cambridge, UK).

Techniques: De-Phosphorylation Assay, Western Blot, MANN-WHITNEY