Journal: Journal of Neurochemistry
Article Title: Functional interplay between ganglioside GM1 and cross‐linking galectin‐1 induces axon‐like neuritogenesis via integrin‐based signaling and TRPC5‐dependent Ca2+ influx
Figure Lengend Snippet: Involvement of TRPC 5 in Ca 2+ influx. NG 108‐15 cells were transfected with TRPC 5‐specific sh RNA or control sh RNA for 48 hr. Suppression of TRPC 5 was demonstrated with RT ‐ PCR (a), immunoblotting (b), and immunocytochemical staining (c). (d) [Ca 2+ ] i measurement showing inhibition of galectin‐1 (Gal‐1)‐induced [Ca 2+ ] i elevation by TRPC 5‐specific sh RNA but not control sh RNA . (e) Cells were treated with the phospholipase C ( PLC ) inhibitor U73122 (1 μM), the PI (3)K inhibitors LY 294002 (5 μM) and wortmannin (2 μM), showing inhibition of Gal‐1‐induced Ca 2+ response.
Article Snippet: For testing involvement of PLC, PI(3)K and TRPC5 channels in Gal‐1‐induced neuritogenesis, pharmacological blockers U73122 (PLC, 1–5 μM), wortmannin (PI(3)K, 1–5 μM), LY294002 (PI(3)K, 1–5 μM) and SK & F 96365 (TRP channel, 10–50 μM) were co‐applied.
Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction, Staining, Inhibition, Planar Chromatography