Journal: Frontiers in Pharmacology
Article Title: 17α-Hydroxyprogesterone Caproate Inhibits Cytokine Production via Suppression of NF-κB Activation
Figure Lengend Snippet: 17-OHPC inhibited PHA-stimulated cytokine production in human PBMCs. PBMCs were seeded into 96-well polypropylene plates at a density of 1.2–2.0 × 10 5 cells/well. After pre-incubation at 37°C for 1 h, 17-OHPC at different concentrations (0.1, 1, and 10 μM) was added to the plates and incubated with the cells for 16 h. Afterwards, PHA (10 μg/ml) was added to the wells and incubated for additional 48 h. Following the incubation, plates were centrifuged, and cell culture supernatants were collected and stored at −80°C until further analysis. The levels of TNF-α, IFN-γ, IL-1β, IL-2, IL-6, and IL-10 in cell culture supernatants were determined using the Human Cytokine/Chemokine Magnetic bead panel from Millipore Sigma (Mean ± SEM, n = 3; * p
Article Snippet: Phytohemagglutinin (PHA) was obtained from Sigma-Aldrich (# 11249738001).
Techniques: Incubation, Cell Culture