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    • pha  (Millipore)
    93
    Millipore pha
    Pha, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pha/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pha - by Bioz Stars, 2022-08
    93/100 stars
    		  Buy from Supplier
    phytohemagglutinin pha  (Millipore)
    86
    Millipore phytohemagglutinin pha
    17-OHPC inhibited <t>PHA-stimulated</t> cytokine production in human PBMCs. PBMCs were seeded into 96-well polypropylene plates at a density of 1.2–2.0 × 10 5 cells/well. After pre-incubation at 37°C for 1 h, 17-OHPC at different concentrations (0.1, 1, and 10 μM) was added to the plates and incubated with the cells for 16 h. Afterwards, PHA (10 μg/ml) was added to the wells and incubated for additional 48 h. Following the incubation, plates were centrifuged, and cell culture supernatants were collected and stored at −80°C until further analysis. The levels of TNF-α, IFN-γ, IL-1β, IL-2, IL-6, and IL-10 in cell culture supernatants were determined using the Human Cytokine/Chemokine Magnetic bead panel from Millipore <t>Sigma</t> (Mean ± SEM, n = 3; * p
    Phytohemagglutinin Pha, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phytohemagglutinin pha/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phytohemagglutinin pha - by Bioz Stars, 2022-08
    86/100 stars
    		  Buy from Supplier
    pha  (Thermo Fisher)
    94
    Thermo Fisher pha
    17-OHPC inhibited <t>PHA-stimulated</t> cytokine production in human PBMCs. PBMCs were seeded into 96-well polypropylene plates at a density of 1.2–2.0 × 10 5 cells/well. After pre-incubation at 37°C for 1 h, 17-OHPC at different concentrations (0.1, 1, and 10 μM) was added to the plates and incubated with the cells for 16 h. Afterwards, PHA (10 μg/ml) was added to the wells and incubated for additional 48 h. Following the incubation, plates were centrifuged, and cell culture supernatants were collected and stored at −80°C until further analysis. The levels of TNF-α, IFN-γ, IL-1β, IL-2, IL-6, and IL-10 in cell culture supernatants were determined using the Human Cytokine/Chemokine Magnetic bead panel from Millipore <t>Sigma</t> (Mean ± SEM, n = 3; * p
    Pha, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pha/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pha - by Bioz Stars, 2022-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    17-OHPC inhibited PHA-stimulated cytokine production in human PBMCs. PBMCs were seeded into 96-well polypropylene plates at a density of 1.2–2.0 × 10 5 cells/well. After pre-incubation at 37°C for 1 h, 17-OHPC at different concentrations (0.1, 1, and 10 μM) was added to the plates and incubated with the cells for 16 h. Afterwards, PHA (10 μg/ml) was added to the wells and incubated for additional 48 h. Following the incubation, plates were centrifuged, and cell culture supernatants were collected and stored at −80°C until further analysis. The levels of TNF-α, IFN-γ, IL-1β, IL-2, IL-6, and IL-10 in cell culture supernatants were determined using the Human Cytokine/Chemokine Magnetic bead panel from Millipore Sigma (Mean ± SEM, n = 3; * p

    Journal: Frontiers in Pharmacology

    Article Title: 17α-Hydroxyprogesterone Caproate Inhibits Cytokine Production via Suppression of NF-κB Activation

    doi: 10.3389/fphar.2022.831315

    Figure Lengend Snippet: 17-OHPC inhibited PHA-stimulated cytokine production in human PBMCs. PBMCs were seeded into 96-well polypropylene plates at a density of 1.2–2.0 × 10 5 cells/well. After pre-incubation at 37°C for 1 h, 17-OHPC at different concentrations (0.1, 1, and 10 μM) was added to the plates and incubated with the cells for 16 h. Afterwards, PHA (10 μg/ml) was added to the wells and incubated for additional 48 h. Following the incubation, plates were centrifuged, and cell culture supernatants were collected and stored at −80°C until further analysis. The levels of TNF-α, IFN-γ, IL-1β, IL-2, IL-6, and IL-10 in cell culture supernatants were determined using the Human Cytokine/Chemokine Magnetic bead panel from Millipore Sigma (Mean ± SEM, n = 3; * p

    Article Snippet: Phytohemagglutinin (PHA) was obtained from Sigma-Aldrich (# 11249738001).

    Techniques: Incubation, Cell Culture