Journal: Scientific Reports
Article Title: Valproate reduces neuroinflammation and neuronal death in a rat chronic constriction injury model
Figure Lengend Snippet: Double immunofluorescent staining for S100 (Schwann cell marker), GFAP (DRG satellite cell marker), OX-42 (spinal microglia marker) and pGSK-3β in the sciatic nerve (SN), dorsal root ganglia (DRG) and spinal cord (SC) on day 7 after chronic constriction injury (CCI). pGSK-3β protein expression was significantly increased in the CCI group compared to sham and CCI + VPA groups. Administration of VPA attenuated CCI-enhanced pGSK-3β proteins. Quantitative fluorescence data are depicted. Data represent the mean ± SEM for 6 rats per group. # p
Article Snippet: The primary antibodies of GSK-3β, pGSK-3β (1:500 dilution, Cell Signaling Technology, Danvers MA), AKT (1:1000 dilution, Cell Signaling Technology), pAKT (Ser473, 1:500 dilution, Cell Signaling Technology), pNFκB (1:500 dilution, Cell Signaling Technology), COX-2 (1:500 dilution, Abcam London, UK), iNOS (1:1000 dilution, Santa Cruz Biotechnology, Santa Cruz CA) and β-actin (1:2000 dilution, Sigma-Aldrich, Saint Louis MO) were used in this experiment.
Techniques: Staining, Marker, Expressing, Fluorescence