pgl2 basic pgl2b luciferase reporter gene vector Promega Search Results


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    Promega pgl2 basic pgl2 b luciferase reporter gene vector
    ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with <t>pGL2B</t> or with <t>pGL2-COL2</t> together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and
    Pgl2 Basic Pgl2 B Luciferase Reporter Gene Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl2 basic pgl2 b luciferase reporter gene vector/product/Promega
    Average 90 stars, based on 17 article reviews
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    pgl2 basic pgl2 b luciferase reporter gene vector - by Bioz Stars, 2020-09
    90/100 stars
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    ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with pGL2B or with pGL2-COL2 together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and

    Journal:

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes

    doi: 10.1002/jcp.21338

    Figure Lengend Snippet: ESE-1 decreases COL2A1 promoter activity and enhances IL-1β-dependent inhibition. The C-28/I2 chondrocytes were cotransfected with pGL2B or with pGL2-COL2 together with pCI empty vector or with (A) increasing amounts of pCI-ESE1 (50, 100, and

    Article Snippet: The COL2A1 sequence spanning –577 to +3,428 bp ( ; ) was cloned into the pGL2-basic (pGL2B) luciferase reporter gene vector (Promega) to generate the pGL2-COL2 construct containing approximately 4 kb of 5′-flanking sequence.

    Techniques: Activity Assay, Inhibition, Plasmid Preparation