pgex-6p-1-eif2β constructs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore lysis buffer
    Lysis Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 95438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer/product/Millipore
    Average 99 stars, based on 95438 article reviews
    Price from $9.99 to $1999.99
    lysis buffer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    93
    GE Healthcare pgex 6p 1
    Pgex 6p 1, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 4760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgex 6p 1/product/GE Healthcare
    Average 93 stars, based on 4760 article reviews
    Price from $9.99 to $1999.99
    pgex 6p 1 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    GE Healthcare pgex 6p 1 vector
    Pgex 6p 1 Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 3415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgex 6p 1 vector/product/GE Healthcare
    Average 92 stars, based on 3415 article reviews
    Price from $9.99 to $1999.99
    pgex 6p 1 vector - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    99
    Millipore tcep
    Tcep, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3077 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tcep/product/Millipore
    Average 99 stars, based on 3077 article reviews
    Price from $9.99 to $1999.99
    tcep - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore bl21 de3 competent e coli
    Bl21 De3 Competent E Coli, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 competent e coli/product/Millipore
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 competent e coli - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    GE Healthcare recombinant proteins
    Recombinant Proteins, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant proteins/product/GE Healthcare
    Average 92 stars, based on 1565 article reviews
    Price from $9.99 to $1999.99
    recombinant proteins - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    GE Healthcare glutathione sepharose 4b
    mTOR and CK2 inhibitors suppress eIF2β phosphorylation, increase phospho-eIF2α levels and interfere with eIF4F complex assembly. ( a ) HEK293E or MCF7 cells were serum starved for 16 h and then stimulated with 10% serum (fetal bovine serum, FBS) in the presence of a vehicle (DMSO), rapamycin (50 nM) or torin1 (250 nM) for 30 min. Phosphorylation status and levels of indicated proteins were monitored by western blotting. β-Actin served as a loading control. ( b ) HEK293E cells were serum starved for 16 h and then stimulated for 30 min with serum (FBS; 10%) in the presence of a vehicle (DMSO), 250 nM torin1, 15 or 50 μM CX-4945. Phosphorylation and expression levels of indicated proteins were monitored by western blotting. β-Actin served as a loading control. ( c ) Extracts of HEK293E cells treated as in b were subjected to m 7 GTP cap pull down (see Methods). Levels and phosphorylation status of indicated proteins in the pulled down material (25%) and inputs (10%) were determined by western blotting. β-Actin served to exclude contamination of m 7 GTP cap pull downs (for example, non-specific binding to the <t>agarose</t> beads) and as a loading control for inputs. Parallel results were obtained in MCF7 and HCT116 cells ( Supplementary Fig. 1a,b ). ( d ) Expression of HA-CK2α in U2OS cells was induced by tetracycline withdrawal (doxycycline was used at 1.5 μg ml −1 to suppress expression of CK2 subunits in control cells). Cells were starved for 16 h after which cells were stimulated by 10% serum (FBS) in the presence of a vehicle (DMSO), 250 nM torin1, 50 μM CX-4945 or a combination thereof for the indicated time period. ( e ) HCT116 PTEN +/+ or PTEN −/− cells were treated with the indicated concentration of CX-4945 for 1 h in the presence of 10% serum (FBS). ( f ) Cells described in e were incubated with DMSO or torin1 (250 nM) for 1 h. ( d – f ) Expression levels and phosphorylation status of indicated proteins were monitored by western blotting. β-Actin served as a loading control. Experiments in this panel were repeated at least two times independently and the representative results are shown. Where appropriate, quantification was performed using densitometry ( Supplementary Fig. 9 ).
    Glutathione Sepharose 4b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 23914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathione sepharose 4b/product/GE Healthcare
    Average 94 stars, based on 23914 article reviews
    Price from $9.99 to $1999.99
    glutathione sepharose 4b - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    mTOR and CK2 inhibitors suppress eIF2β phosphorylation, increase phospho-eIF2α levels and interfere with eIF4F complex assembly. ( a ) HEK293E or MCF7 cells were serum starved for 16 h and then stimulated with 10% serum (fetal bovine serum, FBS) in the presence of a vehicle (DMSO), rapamycin (50 nM) or torin1 (250 nM) for 30 min. Phosphorylation status and levels of indicated proteins were monitored by western blotting. β-Actin served as a loading control. ( b ) HEK293E cells were serum starved for 16 h and then stimulated for 30 min with serum (FBS; 10%) in the presence of a vehicle (DMSO), 250 nM torin1, 15 or 50 μM CX-4945. Phosphorylation and expression levels of indicated proteins were monitored by western blotting. β-Actin served as a loading control. ( c ) Extracts of HEK293E cells treated as in b were subjected to m 7 GTP cap pull down (see Methods). Levels and phosphorylation status of indicated proteins in the pulled down material (25%) and inputs (10%) were determined by western blotting. β-Actin served to exclude contamination of m 7 GTP cap pull downs (for example, non-specific binding to the agarose beads) and as a loading control for inputs. Parallel results were obtained in MCF7 and HCT116 cells ( Supplementary Fig. 1a,b ). ( d ) Expression of HA-CK2α in U2OS cells was induced by tetracycline withdrawal (doxycycline was used at 1.5 μg ml −1 to suppress expression of CK2 subunits in control cells). Cells were starved for 16 h after which cells were stimulated by 10% serum (FBS) in the presence of a vehicle (DMSO), 250 nM torin1, 50 μM CX-4945 or a combination thereof for the indicated time period. ( e ) HCT116 PTEN +/+ or PTEN −/− cells were treated with the indicated concentration of CX-4945 for 1 h in the presence of 10% serum (FBS). ( f ) Cells described in e were incubated with DMSO or torin1 (250 nM) for 1 h. ( d – f ) Expression levels and phosphorylation status of indicated proteins were monitored by western blotting. β-Actin served as a loading control. Experiments in this panel were repeated at least two times independently and the representative results are shown. Where appropriate, quantification was performed using densitometry ( Supplementary Fig. 9 ).

    Journal: Nature Communications

    Article Title: mTORC1 and CK2 coordinate ternary and eIF4F complex assembly

    doi: 10.1038/ncomms11127

    Figure Lengend Snippet: mTOR and CK2 inhibitors suppress eIF2β phosphorylation, increase phospho-eIF2α levels and interfere with eIF4F complex assembly. ( a ) HEK293E or MCF7 cells were serum starved for 16 h and then stimulated with 10% serum (fetal bovine serum, FBS) in the presence of a vehicle (DMSO), rapamycin (50 nM) or torin1 (250 nM) for 30 min. Phosphorylation status and levels of indicated proteins were monitored by western blotting. β-Actin served as a loading control. ( b ) HEK293E cells were serum starved for 16 h and then stimulated for 30 min with serum (FBS; 10%) in the presence of a vehicle (DMSO), 250 nM torin1, 15 or 50 μM CX-4945. Phosphorylation and expression levels of indicated proteins were monitored by western blotting. β-Actin served as a loading control. ( c ) Extracts of HEK293E cells treated as in b were subjected to m 7 GTP cap pull down (see Methods). Levels and phosphorylation status of indicated proteins in the pulled down material (25%) and inputs (10%) were determined by western blotting. β-Actin served to exclude contamination of m 7 GTP cap pull downs (for example, non-specific binding to the agarose beads) and as a loading control for inputs. Parallel results were obtained in MCF7 and HCT116 cells ( Supplementary Fig. 1a,b ). ( d ) Expression of HA-CK2α in U2OS cells was induced by tetracycline withdrawal (doxycycline was used at 1.5 μg ml −1 to suppress expression of CK2 subunits in control cells). Cells were starved for 16 h after which cells were stimulated by 10% serum (FBS) in the presence of a vehicle (DMSO), 250 nM torin1, 50 μM CX-4945 or a combination thereof for the indicated time period. ( e ) HCT116 PTEN +/+ or PTEN −/− cells were treated with the indicated concentration of CX-4945 for 1 h in the presence of 10% serum (FBS). ( f ) Cells described in e were incubated with DMSO or torin1 (250 nM) for 1 h. ( d – f ) Expression levels and phosphorylation status of indicated proteins were monitored by western blotting. β-Actin served as a loading control. Experiments in this panel were repeated at least two times independently and the representative results are shown. Where appropriate, quantification was performed using densitometry ( Supplementary Fig. 9 ).

    Article Snippet: Lysates were incubated with 20 μl of Glutathione Sepharose 4B (GE Healthcare Life Sciences) for 1 h at 4 °C and then washed twice with lysis buffer supplemented with 5% glycerol and 0.1% NP-40, and 700 mM NaCl.

    Techniques: Western Blot, Expressing, Binding Assay, Concentration Assay, Incubation