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  • 99
    GE Healthcare pgex 4t3
    Concept of the asymmetric directional T-vector. ( A ) Construction of <t>pGEX-4T3-PRESAT</t> and direct cloning of PCR product in pGEX-4T3-PRESAT. ( B ) The schematic representation of the ORF selection method using potential restriction enzyme site. The figure illustrates the case in which NcoI is chosen as the second restriction enzyme for selection. The rear PCR primer is designed with 5′-GG at the 5′ end, so that only the ligated plasmid with insert in the reverse orientation will have the NcoI site at the TA-cloning position. For NdeI selection, the rear primer with 5′-ATG is used instead of the 5′-GG primer.
    Pgex 4t3, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare pgex 4t3 plasmid
    Preparation of H228A and D132A mutant proteins of CN2 with different N‐terminal additional sequences. cDNAs encoding inactive CN2 mutants, H228A, and D132A, were cloned into <t>pGEX‐6P3</t> and <t>pGEX‐4T3</t> vectors, respectively (Supporting Information Fig. S2), and expressed in E. coli as GST‐fusion proteins. The recombinant proteins were then trapped on glutathione‐Sepharose beads and digested with PreScission™ or thrombin protease, respectively. The yielded proteins have expected average molecular weights of 53,411 and 53,166 Da, respectively.
    Pgex 4t3 Plasmid, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    GE Healthcare pgex 4t3 prokaryotic expression vectors
    Preparation of H228A and D132A mutant proteins of CN2 with different N‐terminal additional sequences. cDNAs encoding inactive CN2 mutants, H228A, and D132A, were cloned into <t>pGEX‐6P3</t> and <t>pGEX‐4T3</t> vectors, respectively (Supporting Information Fig. S2), and expressed in E. coli as GST‐fusion proteins. The recombinant proteins were then trapped on glutathione‐Sepharose beads and digested with PreScission™ or thrombin protease, respectively. The yielded proteins have expected average molecular weights of 53,411 and 53,166 Da, respectively.
    Pgex 4t3 Prokaryotic Expression Vectors, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    GE Healthcare pgex 4t3 expression vector
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Pgex 4t3 Expression Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 83/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    GE Healthcare pgex tev vector
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Pgex Tev Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    GE Healthcare pgex 4t3 gst fusion vectors
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Pgex 4t3 Gst Fusion Vectors, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    GE Healthcare gst encoding ones
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Gst Encoding Ones, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    GE Healthcare prokaryotic expression vector pgex 4t3
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Prokaryotic Expression Vector Pgex 4t3, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prokaryotic expression vector pgex 4t3/product/GE Healthcare
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    GE Healthcare pgex 4t3 bacterial expression vector
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Pgex 4t3 Bacterial Expression Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    GE Healthcare pgex 4t3 gst fusion vector
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Pgex 4t3 Gst Fusion Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgex 4t3 gst fusion vector/product/GE Healthcare
    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pgex 4t3 gst fusion vector - by Bioz Stars, 2020-02
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    78
    GE Healthcare gst fusion protein expression vector pgex 4t3
    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid <t>pGEX-4T3/BoSA1.</t> Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.
    Gst Fusion Protein Expression Vector Pgex 4t3, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gst fusion protein expression vector pgex 4t3/product/GE Healthcare
    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    gst fusion protein expression vector pgex 4t3 - by Bioz Stars, 2020-02
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    Image Search Results


    Concept of the asymmetric directional T-vector. ( A ) Construction of pGEX-4T3-PRESAT and direct cloning of PCR product in pGEX-4T3-PRESAT. ( B ) The schematic representation of the ORF selection method using potential restriction enzyme site. The figure illustrates the case in which NcoI is chosen as the second restriction enzyme for selection. The rear PCR primer is designed with 5′-GG at the 5′ end, so that only the ligated plasmid with insert in the reverse orientation will have the NcoI site at the TA-cloning position. For NdeI selection, the rear primer with 5′-ATG is used instead of the 5′-GG primer.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: The PRESAT-vector: Asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics

    doi: 10.1110/ps.03439004

    Figure Lengend Snippet: Concept of the asymmetric directional T-vector. ( A ) Construction of pGEX-4T3-PRESAT and direct cloning of PCR product in pGEX-4T3-PRESAT. ( B ) The schematic representation of the ORF selection method using potential restriction enzyme site. The figure illustrates the case in which NcoI is chosen as the second restriction enzyme for selection. The rear PCR primer is designed with 5′-GG at the 5′ end, so that only the ligated plasmid with insert in the reverse orientation will have the NcoI site at the TA-cloning position. For NdeI selection, the rear primer with 5′-ATG is used instead of the 5′-GG primer.

    Article Snippet: Oligonucleotide primers were obtained from Hokkaido System Science. pGEM-T (Promega), pGEX-4T3 (Amersham Bioscience), and pET32a (Novagen), respectively, were purchased.

    Techniques: Plasmid Preparation, Clone Assay, Polymerase Chain Reaction, Selection, TA Cloning

    Concept of HTS for the soluble GST-fusion proteins from protein expression libraries. (i) Various PCR primers were designed and used to amplify a series of partial cDNA fragments of the protein of interest, which may contain a putative domain. (ii) The fragments are inserted into pGEX-4T3-PRESAT vector, selected for ORF orientation by restriction cleavage, and transformed into E. coli for colony isolation. (iii) Deep 96-well format plates are used to culture individual clones to allow expression of the GST-fusion proteins. (iv) The clone displaying the highest CDNB assay signal will be selected for further analysis.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: The PRESAT-vector: Asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics

    doi: 10.1110/ps.03439004

    Figure Lengend Snippet: Concept of HTS for the soluble GST-fusion proteins from protein expression libraries. (i) Various PCR primers were designed and used to amplify a series of partial cDNA fragments of the protein of interest, which may contain a putative domain. (ii) The fragments are inserted into pGEX-4T3-PRESAT vector, selected for ORF orientation by restriction cleavage, and transformed into E. coli for colony isolation. (iii) Deep 96-well format plates are used to culture individual clones to allow expression of the GST-fusion proteins. (iv) The clone displaying the highest CDNB assay signal will be selected for further analysis.

    Article Snippet: Oligonucleotide primers were obtained from Hokkaido System Science. pGEM-T (Promega), pGEX-4T3 (Amersham Bioscience), and pET32a (Novagen), respectively, were purchased.

    Techniques: Expressing, Polymerase Chain Reaction, Plasmid Preparation, Transformation Assay, Isolation, Clone Assay

    Preparation of H228A and D132A mutant proteins of CN2 with different N‐terminal additional sequences. cDNAs encoding inactive CN2 mutants, H228A, and D132A, were cloned into pGEX‐6P3 and pGEX‐4T3 vectors, respectively (Supporting Information Fig. S2), and expressed in E. coli as GST‐fusion proteins. The recombinant proteins were then trapped on glutathione‐Sepharose beads and digested with PreScission™ or thrombin protease, respectively. The yielded proteins have expected average molecular weights of 53,411 and 53,166 Da, respectively.

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Evidence for an essential role of intradimer interaction in catalytic function of carnosine dipeptidase II using electrospray‐ionization mass spectrometry

    doi: 10.1002/pro.2842

    Figure Lengend Snippet: Preparation of H228A and D132A mutant proteins of CN2 with different N‐terminal additional sequences. cDNAs encoding inactive CN2 mutants, H228A, and D132A, were cloned into pGEX‐6P3 and pGEX‐4T3 vectors, respectively (Supporting Information Fig. S2), and expressed in E. coli as GST‐fusion proteins. The recombinant proteins were then trapped on glutathione‐Sepharose beads and digested with PreScission™ or thrombin protease, respectively. The yielded proteins have expected average molecular weights of 53,411 and 53,166 Da, respectively.

    Article Snippet: The cDNA encoding D132A mutant protein was further subcloned into pGEX‐4T3 vector (GE Healthcare) using EcoRI and XhoI.

    Techniques: Mutagenesis, Clone Assay, Recombinant

    SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid pGEX-4T3/BoSA1. Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.

    Journal: Journal of Clinical Microbiology

    Article Title: Identification and Expression of Babesia ovis Secreted Antigen 1 and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

    doi: 10.1128/JCM.03219-14

    Figure Lengend Snippet: SDS-PAGE analysis of the recombinant BoSA1 protein expressed in E. coli DH5α. Lane M, protein ladder (kDa). The predicted rBoSA1 protein band expressed in cells is indicated. The figure includes five randomly selected clones of E. coli DH5α cells transformed with recombinant plasmid pGEX-4T3/BoSA1. Lanes E1, E2, E3, E4, and E5, E. coli DH5α cells treated with IPTG. Lanes C1, C2, C3, C4, and C5, uninduced control E. coli DH5α cells.

    Article Snippet: Using forward (5′-GC GGATCC CTTGGCGGAGACGAGGAT-3′; underlined bases indicate a BamHI site) and reverse (5′-GC CTCGAG TAATGGCATCGGGCAAG-3′; underlined bases indicate a XhoI site) primers designed to contain restriction enzyme sites in the expression vector pGEX-4T3 (Amersham Pharmacia Biotech), Babesia ovis secreted antigen 1 (BoSA1) cDNA was amplified by PCR.

    Techniques: SDS Page, Recombinant, Clone Assay, Transformation Assay, Plasmid Preparation