Article Title: Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells
Figure Lengend Snippet: A) Nucleotide Sequence of the Extracellular Domain of Ovine CD34. The 858bp PCR product (see ) was excised from the gel, purified, and cloned into the pGEM–T Easy vector (Promega), as described in Materials and Methods. Sequencing was performed
Article Snippet: The 858bp band corresponding to the extracellular domain of the sheep CD34 gene was excised from the gel, purified using the Qiaex II kit (Qiagen, Inc., Valencia, CA) and cloned into the pGEM-T Easy vector (Promega, San Luis Obispo, CA), according to manufacturer's instructions.
Techniques: Sequencing, Polymerase Chain Reaction, Purification, Clone Assay, Plasmid Preparation