pgem t easy vector Search Results


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  • 90
    Promega pgem t easy vector systems
    pELMO and <t>pGEM-T</t> Easy vector cloning efficiency for different sized inserts. pELMO and pGEM-T Easy cloning efficiency regarding low, medium and large sized inserts. Cloning efficiency is expressed as the ratio of the amount of PCR positive colonies
    Pgem T Easy Vector Systems, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 11565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 11565 article reviews
    Price from $9.99 to $1999.99
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    90/100 stars
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    96
    Thermo Fisher pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 976 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 976 article reviews
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    96
    tiangen biotech co pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 63 article reviews
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    96
    TaKaRa pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Stratagene pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 88/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bio-Rad pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 17 article reviews
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    Thermo Fisher pgem t easy vector system
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector system - by Bioz Stars, 2020-02
    96/100 stars
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    88
    TaKaRa pgem t easy vector system
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 20 article reviews
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    88
    tiangen biotech co pgem t easy vector system
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector System, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 8 article reviews
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    99
    Promega pgem t easy vectors
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2675 article reviews
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    tiangen biotech co pgem t easy vectors
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vectors, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 87/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 13 article reviews
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    Thermo Fisher pgem t easy vectors
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pmd 19t pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pmd 19t Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 20 article reviews
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    Stratagene plasmids pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Plasmids Pgem T Easy Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Agilent technologies pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Macrogen pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Macrogen, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 10 article reviews
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    Qiagen pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 64 article reviews
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    Eurofins pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Eurofins, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 14 article reviews
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    Bioneer Corporation pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 5 article reviews
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    88
    GATC Biotech pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by GATC Biotech, used in various techniques. Bioz Stars score: 88/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 33 article reviews
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    96
    New England Biolabs pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega xbai sali digested pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Xbai Sali Digested Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega pgem t easy vector system i kit
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector System I Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega cloning kit pgem t easy vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Cloning Kit Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 15 article reviews
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    79
    Thermo Fisher cloning vector pgem t easy
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Cloning Vector Pgem T Easy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co pgem t easy cloning vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Cloning Vector, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgem t easy cloning vector - by Bioz Stars, 2020-02
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    79
    TransGen biotech co pgem t easy cloning vector
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Cloning Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pgem t easy cloning vectors
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Cloning Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 77/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech pgem t easy vector sequence
    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the <t>pGEM-T</t> vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).
    Pgem T Easy Vector Sequence, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 77/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector sequence/product/Sangon Biotech
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    Image Search Results


    pELMO and pGEM-T Easy vector cloning efficiency for different sized inserts. pELMO and pGEM-T Easy cloning efficiency regarding low, medium and large sized inserts. Cloning efficiency is expressed as the ratio of the amount of PCR positive colonies

    Journal: AMB Express

    Article Title: pELMO, an optimised in-house cloning vector

    doi: 10.1186/s13568-017-0324-2

    Figure Lengend Snippet: pELMO and pGEM-T Easy vector cloning efficiency for different sized inserts. pELMO and pGEM-T Easy cloning efficiency regarding low, medium and large sized inserts. Cloning efficiency is expressed as the ratio of the amount of PCR positive colonies

    Article Snippet: Such step is essential for plasmids having T-overhangs, like pGEM-T Easy Vector Systems (Promega Corporation MD, USA).

    Techniques: Plasmid Preparation, Clone Assay, Polymerase Chain Reaction

    A) Nucleotide Sequence of the Extracellular Domain of Ovine CD34. The 858bp PCR product (see ) was excised from the gel, purified, and cloned into the pGEM–T Easy vector (Promega), as described in Materials and Methods. Sequencing was performed

    Journal:

    Article Title: Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells

    doi: 10.1016/j.exphem.2008.09.003

    Figure Lengend Snippet: A) Nucleotide Sequence of the Extracellular Domain of Ovine CD34. The 858bp PCR product (see ) was excised from the gel, purified, and cloned into the pGEM–T Easy vector (Promega), as described in Materials and Methods. Sequencing was performed

    Article Snippet: The 858bp band corresponding to the extracellular domain of the sheep CD34 gene was excised from the gel, purified using the Qiaex II kit (Qiagen, Inc., Valencia, CA) and cloned into the pGEM-T Easy vector (Promega, San Luis Obispo, CA), according to manufacturer's instructions.

    Techniques: Sequencing, Polymerase Chain Reaction, Purification, Clone Assay, Plasmid Preparation

    A. Agarose gel electrophoresis of the digestion of clones containing the PCR products of cTnT cDNAs subcloned into the pGEM-T-Easy vector system. Notice that bands 2 and 4 (from left to right) are smaller than expected, corresponding to TNNT2-2 and TNNT2-3,

    Journal:

    Article Title: Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl

    doi: 10.1016/j.bbrc.2007.03.064

    Figure Lengend Snippet: A. Agarose gel electrophoresis of the digestion of clones containing the PCR products of cTnT cDNAs subcloned into the pGEM-T-Easy vector system. Notice that bands 2 and 4 (from left to right) are smaller than expected, corresponding to TNNT2-2 and TNNT2-3,

    Article Snippet: Reverse primer: 5′-TGCTGCTAGGTCTTCTTGGCAGAGGTG PCR bands with different sizes were isolated from agarose gels and cloned into a T-vector using the pGEM–T Easy Vector System (Promega, WI).

    Techniques: Agarose Gel Electrophoresis, Clone Assay, Polymerase Chain Reaction, Plasmid Preparation

    DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the pGEM-T vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).

    Journal: Molecular Vision

    Article Title: FGFR2 molecular analysis and related clinical findings in one Chinese family with Crouzon Syndrome

    doi:

    Figure Lengend Snippet: DNA sequence of a part of FGF2 in the affected patients and unaffected individuals. A : A mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. B : Sequence of the normal allele of exon 8 subcloned into the pGEM-T vector used as a control. C : A heterozygous missense mutation c.866A > C (Gln289Pro) in exon 8 in the affected individuals. The mutation causes the glutarnine 289 codon (CAG) to change to a proline codon (CCG).

    Article Snippet: The purified PCR fragments were ligated into the pGEM-T easy vector (Invitrogen, Carlsbad, CA), and the resulting plasmids were transfected, by heat shock, into DH5a-competent Escherichia coli for propagation.

    Techniques: Sequencing, Mutagenesis, Plasmid Preparation