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  • 92
    Becton Dickinson c peptide alexa fluor 647
    C Peptide Alexa Fluor 647, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti cd133 apc
    Anti Cd133 Apc, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti cd31 percp cy5 5
    Anti Cd31 Percp Cy5 5, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti cd235a percp cy5 5
    Anti Cd235a Percp Cy5 5, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti ecad pe cy7
    Anti Ecad Pe Cy7, supplied by BioLegend, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti nkx6 1 pe
    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , <t>NKX6-1</t> , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and NKX2-2 ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.
    Anti Nkx6 1 Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MBL International anti gp2 pe
    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of <t>GP2</t> and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.
    Anti Gp2 Pe, supplied by MBL International, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti epcam brillant violet 605
    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of <t>GP2</t> and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.
    Anti Epcam Brillant Violet 605, supplied by BioLegend, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Miltenyi Biotec anti susd2 viobrightfitc
    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of <t>GP2</t> and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.
    Anti Susd2 Viobrightfitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson glucagon bv421
    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of <t>GP2</t> and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.
    Glucagon Bv421, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti cd142 bv711
    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of <t>GP2</t> and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.
    Anti Cd142 Bv711, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems human neurogenin 3 antibody
    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of <t>GP2</t> and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.
    Human Neurogenin 3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Developmental Studies Hybridoma Bank mouse anti nkx2 2
    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and <t>NKX2-2</t> ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.
    Mouse Anti Nkx2 2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti cd45 percp cy5 5
    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and <t>NKX2-2</t> ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.
    Anti Cd45 Percp Cy5 5, supplied by BioLegend, used in various techniques. Bioz Stars score: 89/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellfree Sciences peu mcs e01
    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and <t>NKX2-2</t> ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.
    Peu Mcs E01, supplied by Cellfree Sciences, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Wyatt Technology gel permeation chromatography gpc
    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and <t>NKX2-2</t> ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.
    Gel Permeation Chromatography Gpc, supplied by Wyatt Technology, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno alexa fluor 647 affinipure rabbit anti sheep igg
    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and <t>NKX2-2</t> ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.
    Alexa Fluor 647 Affinipure Rabbit Anti Sheep Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and NKX2-2 ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.

    Journal: Development (Cambridge, England)

    Article Title: Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling

    doi: 10.1242/dev.165480

    Figure Lengend Snippet: Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and NKX2-2 ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.

    Article Snippet: The following antibodies were used: anti-CD45-PerCP/Cy5.5 (1/20, clone 2D1, BioLegend), anti-CD31-PerCP/Cy5.5 (1/20, clone WM59, BioLegend), anti-CD235a-PerCP/Cy5.5 (1/20, clone HI264, BioLegend), anti-EPCAM-Brillant Violet 605 (1/20, clone 9C4, BioLegend), anti-ECAD-PE-Cy7 (1/20, clone 67A4, BioLegend), anti-CD133-APC (1/20, clone 315-2C11, BioLegend), anti-GP2-PE (1/5, clone 3G7H9, MBL International), anti-SUSD2-VioBrightFITC (1/20, W5C5, Miltenyi Biotec), anti-CD142-BV711 (1/20, clone HTF-1, BD Biosciences), anti-NKX6-1-PE (1/40, clone R11-560, BD Biosciences), sheep anti-NEUROG3 (1/300, AF3444, R & D Systems), mouse anti-NKX2-2 (1/200, F4.5A5, Developmental Studies Hybridoma Bank), C-peptide-Alexa Fluor 647 (1/200, clone U8-424, BD Biosciences) and Glucagon-BV421 (1/80, clone U16-850, BD Biosciences).

    Techniques: Expressing

    Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of GP2 and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.

    Journal: Development (Cambridge, England)

    Article Title: Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling

    doi: 10.1242/dev.165480

    Figure Lengend Snippet: Characterization of sorted cell populations from human fetal pancreata by RNA-seq at 9 WD. (A) Flow cytometry analysis of the expression of GP2 and ECAD on CD45 − CD31 − EPCAM + cells at 9 WD. CD142 and SUSD2 expression was analyzed in GP2 − ECAD + and GP2 − ECAD − cells. FACS plots are representative of three independent pancreata at 9 WD. (B) Scheme of the experimental setup: GP2 − ECAD + CD142 + SUSD2 − (population A, yellow), GP2 − ECAD + CD142 − SUSD2 − (population B, orange), GP2 − ECAD low CD142 − SUSD2 + (population C, red) and GP2 − ECAD low CD142 − SUSD2 − (population D, blue) were cell sorted and analyzed by RNA-seq. All populations were CD45 − CD31 − EPCAM + highlights genes enriched in the four sorted cell populations.

    Article Snippet: The following antibodies were used: anti-CD45-PerCP/Cy5.5 (1/20, clone 2D1, BioLegend), anti-CD31-PerCP/Cy5.5 (1/20, clone WM59, BioLegend), anti-CD235a-PerCP/Cy5.5 (1/20, clone HI264, BioLegend), anti-EPCAM-Brillant Violet 605 (1/20, clone 9C4, BioLegend), anti-ECAD-PE-Cy7 (1/20, clone 67A4, BioLegend), anti-CD133-APC (1/20, clone 315-2C11, BioLegend), anti-GP2-PE (1/5, clone 3G7H9, MBL International), anti-SUSD2-VioBrightFITC (1/20, W5C5, Miltenyi Biotec), anti-CD142-BV711 (1/20, clone HTF-1, BD Biosciences), anti-NKX6-1-PE (1/40, clone R11-560, BD Biosciences), sheep anti-NEUROG3 (1/300, AF3444, R & D Systems), mouse anti-NKX2-2 (1/200, F4.5A5, Developmental Studies Hybridoma Bank), C-peptide-Alexa Fluor 647 (1/200, clone U8-424, BD Biosciences) and Glucagon-BV421 (1/80, clone U16-850, BD Biosciences).

    Techniques: RNA Sequencing Assay, Flow Cytometry, Cytometry, Expressing, FACS

    Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and NKX2-2 ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.

    Journal: Development (Cambridge, England)

    Article Title: Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling

    doi: 10.1242/dev.165480

    Figure Lengend Snippet: Characterization of populations A, B, C and D at 9 WD. Heatmaps displaying the expression of multipotent genes ( PDX1 , NKX6-1 , SOX9 and ONECUT1 ) (A); progenitor genes and early endocrine markers ( PAX6 , PAX4 , NEUROD1 , ACOT7 , NEUROG3 , ARX , FEV , ETV1 and NKX2-2 ) enriched in population C (B); Endocrine genes ( IAPP , MAFA , FFAR1 , G6PC2 , ISL1 , PCSK1 , MAFB , GCK and PCSK2 ) enriched in population D (C); and pancreatic hormones ( PPY , GHRL , INS , GCG and SST ) in sorted populations A, B, C and D (D). Heatmaps are displayed using a log2 expression scale.

    Article Snippet: The following antibodies were used: anti-CD45-PerCP/Cy5.5 (1/20, clone 2D1, BioLegend), anti-CD31-PerCP/Cy5.5 (1/20, clone WM59, BioLegend), anti-CD235a-PerCP/Cy5.5 (1/20, clone HI264, BioLegend), anti-EPCAM-Brillant Violet 605 (1/20, clone 9C4, BioLegend), anti-ECAD-PE-Cy7 (1/20, clone 67A4, BioLegend), anti-CD133-APC (1/20, clone 315-2C11, BioLegend), anti-GP2-PE (1/5, clone 3G7H9, MBL International), anti-SUSD2-VioBrightFITC (1/20, W5C5, Miltenyi Biotec), anti-CD142-BV711 (1/20, clone HTF-1, BD Biosciences), anti-NKX6-1-PE (1/40, clone R11-560, BD Biosciences), sheep anti-NEUROG3 (1/300, AF3444, R & D Systems), mouse anti-NKX2-2 (1/200, F4.5A5, Developmental Studies Hybridoma Bank), C-peptide-Alexa Fluor 647 (1/200, clone U8-424, BD Biosciences) and Glucagon-BV421 (1/80, clone U16-850, BD Biosciences).

    Techniques: Expressing

    CD133 marks human fetal pancreatic ductal cells. (A) Flow cytometry analysis displaying the expression of CD133 in populations B, C and D at 11 WD. FACS plots are representative of three independent pancreata at 11 WD. (B) Cell frequencies of population B CD133 + , population C CD133 + and population D CD133 + from three independent pancreata at 10 WD. (C) Expression of CFTR , CHGA , NEUROG3 and NKX2-2 (9-11 WD) by RT-qPCR in population B CD133 + , population B CD133 − , population C CD133 − , population D CD133 − and population D CD133 + . * P

    Journal: Development (Cambridge, England)

    Article Title: Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling

    doi: 10.1242/dev.165480

    Figure Lengend Snippet: CD133 marks human fetal pancreatic ductal cells. (A) Flow cytometry analysis displaying the expression of CD133 in populations B, C and D at 11 WD. FACS plots are representative of three independent pancreata at 11 WD. (B) Cell frequencies of population B CD133 + , population C CD133 + and population D CD133 + from three independent pancreata at 10 WD. (C) Expression of CFTR , CHGA , NEUROG3 and NKX2-2 (9-11 WD) by RT-qPCR in population B CD133 + , population B CD133 − , population C CD133 − , population D CD133 − and population D CD133 + . * P

    Article Snippet: The following antibodies were used: anti-CD45-PerCP/Cy5.5 (1/20, clone 2D1, BioLegend), anti-CD31-PerCP/Cy5.5 (1/20, clone WM59, BioLegend), anti-CD235a-PerCP/Cy5.5 (1/20, clone HI264, BioLegend), anti-EPCAM-Brillant Violet 605 (1/20, clone 9C4, BioLegend), anti-ECAD-PE-Cy7 (1/20, clone 67A4, BioLegend), anti-CD133-APC (1/20, clone 315-2C11, BioLegend), anti-GP2-PE (1/5, clone 3G7H9, MBL International), anti-SUSD2-VioBrightFITC (1/20, W5C5, Miltenyi Biotec), anti-CD142-BV711 (1/20, clone HTF-1, BD Biosciences), anti-NKX6-1-PE (1/40, clone R11-560, BD Biosciences), sheep anti-NEUROG3 (1/300, AF3444, R & D Systems), mouse anti-NKX2-2 (1/200, F4.5A5, Developmental Studies Hybridoma Bank), C-peptide-Alexa Fluor 647 (1/200, clone U8-424, BD Biosciences) and Glucagon-BV421 (1/80, clone U16-850, BD Biosciences).

    Techniques: Flow Cytometry, Cytometry, Expressing, FACS, Quantitative RT-PCR