pet30a vector Search Results


pet30a  (Promega)
90
Promega pet30a
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+vector/pmc03587985-84-10-17?v=Promega
Average 90 stars, based on 1 article reviews
pet30a - by Bioz Stars, 2026-06
90/100 stars
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pet-30a-smaldh_312 vector  (LGC Genomics GmbH)
90
LGC Genomics GmbH pet-30a-smaldh_312 vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet 30a Smaldh 312 Vector, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+vector/10__1016_slash_j__ejbt__2021__08__002-69-19-25?v=LGC+Genomics+GmbH
Average 90 stars, based on 1 article reviews
pet-30a-smaldh_312 vector - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pet-30a(+) expression vector  (Solarbio Inc)
90
Solarbio Inc pet-30a(+) expression vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet 30a(+) Expression Vector, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+vector/pmc09570420-203-9-14?v=Solarbio+Inc
Average 90 stars, based on 1 article reviews
pet-30a(+) expression vector - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
vector pet30a  (ACGT Inc)
90
ACGT Inc vector pet30a
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Vector Pet30a, supplied by ACGT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+vector/pm27548281-67-10-4?v=ACGT+Inc
Average 90 stars, based on 1 article reviews
vector pet30a - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pet30a vector  (EZBiolab Inc)
90
EZBiolab Inc pet30a vector
Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with <t>pET30a-</t> FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.
Pet30a Vector, supplied by EZBiolab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+vector/pm23116486-27-11-13?v=EZBiolab+Inc
Average 90 stars, based on 1 article reviews
pet30a vector - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with pET30a- FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.

Journal: International Journal of Molecular Sciences

Article Title: High-Yield Production in Escherichia coli of Fungal Immunomodulatory Protein Isolated from Flammulina velutipes and Its Bioactivity Assay in Vivo

doi: 10.3390/ijms14022230

Figure Lengend Snippet: Duration of the expression of His- FIP-fve fusion protein. Lysates were obtained from E. coli (DE3) cells with pET30a- FIP-fve induced by 1 mM Isopropyl β-d-thiogalactoside (IPTG) and were analyzed on 12% SDS-PAGE. Lane M was a molecular weight marker. Lanes 1 to 4 were the samples induced for 0, 1.5, 3.0, and 4.5 h, respectively. The arrow indicated the target protein His- FIP-fve . 10 μL sample was used in SDS-PAGE after centrifugation.

Article Snippet: The PCR product was gel purified and then ligated into pET30a (+) at the Bam HI (R602A, Promega) and Eco RI (R6011, Promega) sites.

Techniques: Expressing, SDS Page, Molecular Weight, Marker, Centrifugation