Journal: Frontiers in Physiology
Article Title: Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei
Figure Lengend Snippet: The inhibitory effects of LvCHH1 and LvCHH2 on the expression of LvIAG . (A) Expression level of LvIAG after unilateral eyestalk ablation. (B) Expression levels of LvIAG after LvCHH1 knockdown. (C) Expression levels of LvIAG after LvCHH2 knockdown. dsEGFP, injected with dsEGFP; dsCHH1, injected with dsCHH1; dsCHH2, injected with dsCHH2. (D) The recombination proteins of LvCHHs. 1–3, inclusion body; 1, pET30a; 2, rCHH1; 3, rCHH2; 4–6, supernatant; 4, pET30a; 5, rCHH1; 6, rCHH2; M, protein ladder. (E) The recombination proteins of LvCHHs after purification and refolding. 1, rCHH1; 2, rCHH2; M, protein ladder. (F) Expression levels of LvIAG after injection with rCHH1 and rCHH2. PBS, injected with PBS; rCHH1, injected with rCHH1; rCHH2, injected with rCHH2. Expression levels of target genes were detected in three replicates. Significant differences of the gene expression levels between two treatments were shown with a star ( ∗ ) at P
Article Snippet: Primers LvCHH1-rpF/rpR, LvCHH2-rpF/rpR were designed with 15 bp extension homologous of linearized pET30a vector (Novogen, France) ends at both ends to amply the ORF of LvCHH1 and LvCHH2 excluding sequence encoding signal peptide, respectively.
Techniques: Expressing, Injection, Purification