pet21d plasmid Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Millipore plasmid pet21d
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Plasmid Pet21d, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pet21d/product/Millipore
    Average 88 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    plasmid pet21d - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    88
    Merck KGaA pet21d plasmid
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d Plasmid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d plasmid/product/Merck KGaA
    Average 88 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    pet21d plasmid - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    85
    Millipore pet21d modified plasmid
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d Modified Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d modified plasmid/product/Millipore
    Average 85 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    pet21d modified plasmid - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    86
    Millipore plasmid pet 21d
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Plasmid Pet 21d, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pet 21d/product/Millipore
    Average 86 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    plasmid pet 21d - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    88
    Thermo Fisher pet21d plasmid
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d plasmid/product/Thermo Fisher
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pet21d plasmid - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    90
    Merck & Co pet21d plasmid vector
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d Plasmid Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d plasmid vector/product/Merck & Co
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pet21d plasmid vector - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    90
    Millipore pet21d
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d/product/Millipore
    Average 90 stars, based on 746 article reviews
    Price from $9.99 to $1999.99
    pet21d - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    90
    Addgene inc pet21d
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d/product/Addgene inc
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pet21d - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    85
    Millipore pet21d dna vector
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Pet21d Dna Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet21d dna vector/product/Millipore
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    pet21d dna vector - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    Millipore overexpression vector pet21d
    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a <t>pET21d</t> derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.
    Overexpression Vector Pet21d, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/overexpression vector pet21d/product/Millipore
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    overexpression vector pet21d - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a pET21d derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.

    Journal: Nature chemical biology

    Article Title: A water-soluble DsbB variant that catalyzes disulfide bond formation in vivo

    doi: 10.1038/nchembio.2409

    Figure Lengend Snippet: Solubilized DsbB variants promote folding of alkaline phosphatase ( a ) Schematic showing disulfide bond connectivity for E. coli alkaline phosphatase, PhoA (2 disulfide bonds depicted by yellow circles connected by yellow lines). Alkaline phosphatase activity measured in periplasmic (white bars) and cytoplasmic (gray bars) fractions derived from the following strains: wt DHB4(DE3) cells (lacking the native phoA gene) carrying no plasmid (empty) or pBAD18-pPhoA (pPhoA); DHB4(DE3) Δ dsbAB cells carrying no plasmid (empty), pBAD18-pPhoA (pPhoA), pFH273 (cPhoA + cDsbA), or pFH273mut (cPhoA + cDsbA(APHA)), along with a pET21d derivative containing SxDsbB, SxDsbBΔC GZ , SxDsbBΔC GZ (C44A), or H 0 DsbB 1/9b as indicated; and SHuffle cells carrying no plasmid (empty) or pFH-cPhoA (cPhoA). Data is the mean of biological triplicates and the error bars represent the standard error of the mean (SEM). ( b ) Western blot analysis of the same periplasmic (p) and cytoplasmic (c) fractions assayed in ( a ) as indicated. Blots were probed with anti-PhoA antibody to detect PhoA (top panel) and anti-GroEL antibody to detect GroEL (bottom panel), which served as a cytoplasmic fractionation marker and loading control. Molecular weight (MW) markers are shown on the left. See Supplementary Figure 10 for uncropped versions of the blot images.

    Article Snippet: For expression and purification of different DsbB chimeras, the plasmid pET21d (Novagen) was used.

    Techniques: Activity Assay, Derivative Assay, Plasmid Preparation, Western Blot, Fractionation, Marker, Molecular Weight

    Visualization of HRP IBs by SDS-PAGE. Lane 1, SeeBlue Plus2 Pre-stained protein standard; lane 2, pET21d + in BL21(DE3) negative control; lane 3, pET21d + /HRP in BL21(DE3); lane 4, IMAC eluate; lane 5, refolding mixture; lane 6, concentrated refolded HRP.

    Journal: Journal of biotechnology

    Article Title: A comparative approach to recombinantly produce the plant enzyme horseradish peroxidase in Escherichia coli

    doi: 10.1016/j.jbiotec.2017.03.003

    Figure Lengend Snippet: Visualization of HRP IBs by SDS-PAGE. Lane 1, SeeBlue Plus2 Pre-stained protein standard; lane 2, pET21d + in BL21(DE3) negative control; lane 3, pET21d + /HRP in BL21(DE3); lane 4, IMAC eluate; lane 5, refolding mixture; lane 6, concentrated refolded HRP.

    Article Snippet: For intracellular production, vector pET21d+ (Novagen, San Diego, USA) and for translocation into the periplasm vectors pAES30, pAES31, pAES33 (AthenaES, Baltimore, USA) and pET39b+ (Novagen, San Diego, USA), providing different signal peptides, were used ( ).

    Techniques: SDS Page, Staining, Negative Control