pet-28a vector Millipore Search Results


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  • 82
    Millipore plasmid vector pet 28a
    Plasmid Vector Pet 28a, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Detection of intracellular c-di-AMP in E. coli cultures harboring the empty vector <t>pET28a</t> or the expression vector pGP1973 (CdaA), pGP1974 (CdaS), or pGP1975 (CdaS L44F ). Cells were grown in LB medium, and cell extracts were prepared as described under
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    Detection of intracellular c-di-AMP in E. coli cultures harboring the empty vector <t>pET28a</t> or the expression vector pGP1973 (CdaA), pGP1974 (CdaS), or pGP1975 (CdaS L44F ). Cells were grown in LB medium, and cell extracts were prepared as described under
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    Image Search Results


    Detection of intracellular c-di-AMP in E. coli cultures harboring the empty vector pET28a or the expression vector pGP1973 (CdaA), pGP1974 (CdaS), or pGP1975 (CdaS L44F ). Cells were grown in LB medium, and cell extracts were prepared as described under

    Journal: The Journal of Biological Chemistry

    Article Title: Cyclic Di-AMP Homeostasis in Bacillus subtilis

    doi: 10.1074/jbc.M112.395491

    Figure Lengend Snippet: Detection of intracellular c-di-AMP in E. coli cultures harboring the empty vector pET28a or the expression vector pGP1973 (CdaA), pGP1974 (CdaS), or pGP1975 (CdaS L44F ). Cells were grown in LB medium, and cell extracts were prepared as described under

    Article Snippet: For this purpose, the cdaA and cdaS genes were amplified using the primer pairs FX68/FX69 and FX71/FX72, respectively, and cloned between the NdeI and BamHI sites of the expression vector pET28a (Novagen).

    Techniques: Plasmid Preparation, Expressing