Journal: Microbial Cell Factories
Article Title: Tailoring the properties of (catalytically)-active inclusion bodies
Figure Lengend Snippet: Microscopy images illustrating CatIB/FIB formation in E. coli . a – f Phase-contrast and fluorescence images of E. coli cells expressing a TDoT-L-YFP, b TDoT-YFP, c 3HAMP-L-YFP, d TDoT-L-mCherry, e TDoT-mCherry, and f 3HAMP-mCherry. g – k phase-contrast images of TDoT fusion (left) and 3HAMP fusion (right) expressing E. coli cells containing g R ADH, h Lb ADH, i Pf BAL, j Pp BFD, k Ec LDC (here, the coiled-coil domain is fused C-terminally), and l E. coli BL21(DE3) with empty pET-28a vector. All strains were grown under standard growth conditions as described in “ Methods ” section
Article Snippet: If not stated otherwise, all gene fusions consisted of gene fragments coding for a coiled-coil domain (here TDoT or 3HAMP), a linker polypeptide, consisting of a protease Factor Xa cleavage site and a triple (GGGS)3 and the respective target proteins/enzymes cloned into a pET-28a vector (Novagen, Merck KGaA, Frankfurt, Germany).
Techniques: Microscopy, Fluorescence, Expressing, Positron Emission Tomography, Plasmid Preparation