Journal: Frontiers in Physiology

Article Title: Paracrine Anti-inflammatory Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in Human Monocytes

doi: 10.3389/fphys.2018.00661

Figure Lengend Snippet: Myeloperoxidase release by human monocytes. Myeloperoxidase activity in cell culture supernatants was measured by a fluorometric procedure after 3 h (A) or 24 h (B) of TPA stimulation in the presence or absence of CM (mean ± SD from 4 separate experiments with cells from separate donors). ++ P

Article Snippet: Myeloperoxidase Monocytes were incubated with CM and/or 12-O-tetradecanoylphorbol-13-acetate (TPA) (300 nM, Sigma-Aldrich) at 37°C for 3 or 24 h. Supernatants were used to measure myeloperoxidase activity by using 3,3′,5,5′-tetramethylbenzidine (Sigma-Aldrich) as substrate as previously described ( ).

Techniques: Activity Assay, Cell Culture

Journal: Nephrology Dialysis Transplantation

Article Title: Cisplatin-induced renal inflammation is ameliorated by cilastatin nephroprotection

doi: 10.1093/ndt/gfx005

Figure Lengend Snippet: Effects of cilastatin on cisplatin-induced inflammatory cell infiltration. Localization of CD68 (monocyte/macrophage) in kidney sections of ( A ) control rats, ( B ) cisplatin, ( C ) control + cilastatin and ( D ) cisplatin + cilastatin. Note increased staining in cisplatin-injected rats (arrows) compared with cisplatin + cilastatin and control rats (magnification 20×); bar = 100 µm. ( E ) Quantification of CD68 immunostaining in renal cells. ( F ) Serum myeloperoxidase (MPO) activity in the groups of rats studied. Administration of cisplatin increased systemic MPO activity. This change was significantly improved with cilastatin. All results are expressed as mean ± SEM; n = 7–8 animals per group. cil, cilastatin. *P

Article Snippet: Myeloperoxidase (MPO) activity was measured in serum using the MPO Colorimetric Activity Assay Kit (Sigma-Aldrich, St Louis, MO, USA) according to the manufacturer’s instructions.

Techniques: Staining, Injection, Immunostaining, Activity Assay

Journal: The Journal of Bone and Joint Surgery. American Volume

Article Title: Single-Dose, Preoperative Vitamin-D Supplementation Decreases Infection in a Mouse Model of Periprosthetic Joint Infection

doi: 10.2106/JBJS.16.01598

Figure Lengend Snippet: Figs. 5-A and 5-B Myeloperoxidase (MPO) and β-N-acetylglucosaminidase (NAG) activity induced by the Xen36 Staphylococcus aureus strain in 25D 3 -deficient, sufficient, and rescued mice. Fig. 5-A Mean MPO activity of the infected joint-tissue specimens (and standard error of the mean [SEM]) (n = 3 mice per group; p

Article Snippet: The homogenate was assayed for myeloperoxidase (MPO) activity levels, a surrogate for neutrophil infiltration, using the Myeloperoxidase Colorimetric Activity Assay Kit (Sigma-Aldrich) according to the manufacturer’s instructions and expressed as milliunits/mL .

Techniques: Activity Assay, Mouse Assay, Infection

Journal: Transplantation

Article Title: Bruton’s tyrosine kinase inhibition attenuates liver damage in a mouse warm ischemia and reperfusion model

doi: 10.1097/TP.0000000000001552

Figure Lengend Snippet: Inhibition of Btk Signaling Reduces IR-Triggered Liver Neutrophil and Lymphocyte Infiltration. (A) Histopathological analysis of liver tissues after 6 hours of IRI. Liver sections were evaluated in a blinded fashion by counting labeled cells within 10 high-power fields per section. BTKB66 led to less neutrophil and T cell infiltration. (B) Myeloperoxidase activity in liver tissue. * P

Article Snippet: Myeloperoxidase (MPO) activity was measured using a Myeloperoxidase Colorimetric Activity Assay Kit (Sigma, St. Louis, MO) according to manufacturer instructions.

Techniques: Inhibition, Labeling, Activity Assay

Journal: Journal of Lipid Research

Article Title: Modulation of the gut microbiota impacts nonalcoholic fatty liver disease: a potential role for bile acids [S]

doi: 10.1194/jlr.M075713

Figure Lengend Snippet: Stimulation of the gut microbiota by GG is associated with reduced hepatic steatosis, but enhanced hepatic inflammation. A: H E staining of representative liver sections. Arrows denote inflammatory infiltrate and inset a higher magnification of the inflammatory infiltrate as observed in the livers of the GG mice. B: Representative pictures of Oil Red O staining of liver sections. C: Quantitative analysis of liver triglyceride levels. D: Activity of ALT in plasma. E: Liver myeloperoxidase (MPO) activity representing hepatic neutrophil content. F: Representative pictures of liver sections stained for the macrophage marker Cd68. G: Relative expression of inflammatory genes in the liver. Gene expression levels in CTRL mice were set at one. Data are presented as mean ± SEM. Asterisks indicate significantly different compared with CTRL. * P

Article Snippet: For hepatic myeloperoxidase activity, liver homogenates were prepared and myeloperoxidase peroxidase activity was measured according to manufacturer’s instructions (Abcam).

Techniques: Staining, Mouse Assay, Activity Assay, Marker, Expressing

Journal: Arthritis Research & Therapy

Article Title: Recombinant human proteoglycan-4 reduces phagocytosis of urate crystals and downstream nuclear factor kappa B and inflammasome activation and production of cytokines and chemokines in human and murine macrophages

doi: 10.1186/s13075-018-1693-x

Figure Lengend Snippet: Impact of recombinant human proteoglycan-4 (rhPRG4) treatment on differential weight bearing (Right hind limb – Left hind limb; R-L) and synovial fluid (SF) lavage myeloperoxidase (MPO) activity following intra-articular administration of monosodium urate monohydrate (MSU) crystals (50μL; 2.5 mg/mL) in the right knee joint of male Lewis rats followed by intra-articular treatments with rhPRG4 (1 mg/mL; 50 μL) or PBS (50 μL) at 1 h following MSU administration, or no treatment. Differential weight bearing was measured at 3 h ( n = 12 in MSU alone and n = 14 in rhPRG4 or PBS treatments), 6 h ( n = 12 in MSU alone and n = 14 in rhPRG4 or PBS treatments) and 24 h ( n = 5 in MSU alone and n = 7 in rhPRG4 or PBS treatments) following MSU administration. SF lavage MPO activities were determined at 6 h ( n = 7 in each group) and 24 h ( n = 5 in each group) following MSU administration. Data are presented as a scatterplot with the mean value highlighted. * p

Article Snippet: Myeloperoxidase (MPO) activity in SF lavage samples was measured using a commercially available kit (Abcam).

Techniques: Recombinant, Activity Assay

Journal: The Journal of Biological Chemistry

Article Title: Ceruloplasmin Is an Endogenous Inhibitor of Myeloperoxidase *

doi: 10.1074/jbc.M112.418970

Figure Lengend Snippet: Reactions of myeloperoxidase in the presence and absence of ceruloplasmin. A , ferric myeloperoxidase ( MPO ) reacts with hydrogen peroxide to form the redox intermediate Compound I, which either oxidizes chloride by removing two electrons or removes a single electron from an organic substrate ( RH ), such as urate, ascorbate, tyrosine, or serotonin, to produce an organic radical ( R • ) and Compound II. Compound II is also reduced by these substrates to produce a second radical and regenerate ferric MPO. Only a single monomer of myeloperoxidase is illustrated. B , a molecule of myeloperoxidase ( MPO ) consisting of two identical dimers, each containing a heme prosthetic group, binds reversibly to two molecules of ceruloplasmin ( CP ). C , reaction of hydrogen peroxide with myeloperoxidase bound to ceruloplasmin enhances the one-electron reduction of Compound I to Compound II. Reduction of Compound II is slowed until it dissociates from ceruloplasmin.

Article Snippet: Antibodies to myeloperoxidase and ceruloplasmin were from Abcam (Cambridge, UK), and goat anti-rabbit immunoglobulin biotin conjugate secondary antibodies were from Dako (Campbellfield, Australia).

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Ceruloplasmin Is an Endogenous Inhibitor of Myeloperoxidase *

doi: 10.1074/jbc.M112.418970

Figure Lengend Snippet: Proteins in Human Plasma Bind Reversibly to Myeloperoxidase and Inhibit Its Activity

Article Snippet: Antibodies to myeloperoxidase and ceruloplasmin were from Abcam (Cambridge, UK), and goat anti-rabbit immunoglobulin biotin conjugate secondary antibodies were from Dako (Campbellfield, Australia).

Techniques: Activity Assay

Journal: Oncotarget

Article Title: An AML1-ETO/miR-29b-1 regulatory circuit modulates phenotypic properties of acute myeloid leukemia cells

doi: 10.18632/oncotarget.18127

Figure Lengend Snippet: Expression of miR-29b-1 partially relieves differentiation block in leukemia cells A. Activity of myeloperoxidase, a marker for promyelocytic differentiation, in SKNO-1 cells expressing indicated miRs is shown. miR-29b-1 causes a modest, but significant increase in MPO activity (denoted by * ( p

Article Snippet: Myeloperoxidase (MPO) activity assay Activity of the MPO enzyme was determined using Myeloperoxidase Activity Colorimetric Assay Kit (Cat. No. ab105136) from Abcam (Cambridge, MA) according to manufacturer's protocol.

Techniques: Expressing, Blocking Assay, Activity Assay, Marker

Journal: Journal of Translational Medicine

Article Title: AQP5-1364A/C polymorphism and the AQP5 expression influence sepsis survival and immune cell migration: a prospective laboratory and patient study

doi: 10.1186/s12967-016-1079-2

Figure Lengend Snippet: Neutrophil migration in peritoneum and lung 3 h after LPS or saline injection i.p. (20 mg/kg). a Total cell count in the peritoneum was 1.2 * 10 6 cells/ml in median in Aqp5 - KO mice compared to 2.5 * 10 6 cells/ml in WT mice (n = 8; p = 0.04). b Representative dot plots show flow cytometric analysis of CD11c and Ly6G/C positive cells in the peritoneum. CD11c Ly6G/C positive cells were increased in WT mice compared to Aqp5 -KO mice after LPS injection c Percentage of CD11c Ly6G/C positive cells in the peritoneum. In WT mice (n = 4) percentage of CD11c Ly6G/C positive cells significantly increased after LPS injection compared to saline injection (p = 0.017) and was higher compared to Aqp5-KO mice (n = 4) (p = 0.035). d Total cell count of CD11c Ly6G/C positive cells (n = 8; p = 0.04). e Representative NASDCL-Esterase staining of formalin fixed lung tissues (40× magnification). WT mice (n = 2) showed higher number of neutrophils compared to Aqp5 -KO (n = 2) mice ( red arrows ) after 3 h LPS injection f Myeloperoxidase (MPO) activity in mice lungs. In WT mice (n = 4) MPO activity significantly increased after LPS injection compared to saline (p = 0.04) and was higher compared to Aqp5-KO mice [n = 4 (p = 0.004) f ]. Data are shown as boxplots (min to max; n = 8 in total)

Article Snippet: MPO activity was determined by the use of Myeloperoxidase activity assay kit (abcam, Cambridge, UK).

Techniques: Migration, Injection, Cell Counting, Mouse Assay, Flow Cytometry, Staining, Activity Assay