Journal: Journal of Neuroinflammation

Article Title: Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury

doi: 10.1186/s12974-018-1222-5

Figure Lengend Snippet: Myeloperoxidase staining of neutrophils in the axotomized SCG 1, 3, and 7 days after axotomy. Immunohistochemical staining of neutrophils with an antibody against MPO showed comparable neutrophil cell counts between WT and Ccr2 −/− SCG 1 and 3 days after injury and a significantly higher number of cells in WT ganglia compared to mutants 7 days after injury. Cell counts were performed using ImageJ at 1, 3, and 7 days post injury ( a ). The counts represent the sum of cells from three images per section. Representative images of IHC staining in the SCG at 1, 3, and 7 days post axotomy are shown for WT Ax ( b , d , f ) and Ccr2 −/− Ax ( c , e , g ). All images were taken at × 25 magnification. Scale bar = 20 μm. n = 5 per group. * p

Article Snippet: IHC was also performed to label neutrophils in the ganglia using a rabbit polyclonal antibody to myeloperoxidase (MPO; 1:100; Abcam, Cambridge, UK) overnight at 4 °C and a Cy3 donkey anti-rabbit secondary antibody (1:400; Jackson ImmunoResearch Laboratories, Inc.).

Techniques: Staining, Immunohistochemistry

Journal: Bioscience Reports

Article Title: Vitamin D ameliorates impaired wound healing in streptozotocin-induced diabetic mice by suppressing NF-κB-mediated inflammatory genes

doi: 10.1042/BSR20171294

Figure Lengend Snippet: Vitamin D reduced inflammation cells infiltration, stimulated cellular proliferation, and augmented neovascularization on the wound bed ( A ) Populations of neutrophil and macrophage at the wound site by determining the molecular markers MPO (neutrophil) and CD68 (macrophage) at day 14. Cellular proliferation in the wound tissues was detected with immunostaining of Ki67. The neovascularization of wounds was detected by immunostaining of endothelial marker CD31. ( B ) Scores of MPO, CD68, ki67, and CD31 staining; n =5 for each group. Data are represented as means ± S.D. * P

Article Snippet: The sections were also incubated with the primary antibody against CD31 (1:50, Abcam, U.S.A.) to observe angiogenesis, ki67 (1:50, Abcam, U.S.A.) to observe proliferation in the ulcerative tissues, MPO (1:50, Abcam, U.S.A.), and CD68 (1:50, Abcam, U.S.A.) to observe infiltration of neutrophils and macrophages in the ulcerative tissues.

Techniques: Immunostaining, Marker, Staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: A single high-fat meal provokes pathological erythrocyte remodeling and increases myeloperoxidase levels: implications for acute coronary syndrome

doi: 10.1038/s41374-018-0038-3

Figure Lengend Snippet: Changes in MPO activity following the HFM and ICM and monocitic exposure to neutral fat. a Individual plasma MPO activity levels before (pre) and after (post) the HFM and ICM. b Pre and post plasma MPO activity following the HFM (top) and ICM (bottom) in EDTA or heparin anticoagulated samples. c Relative changes in MPO activity and inflammatory marker expression (ELISA) in response to THP-1 monocyte exposure to neutral fat for 4 h. *Significant from pre. **Significant from untreated. † Significant from EDTA post ( n = 6)

Article Snippet: While there was an increase in MPO activity in the EDTA-collected samples post HFM, collection of these samples into heparin resulted in a further increase in MPO activity, indicative of liberation from RBCs ( ).

Techniques: Activity Assay, Marker, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: Protective Effect of Pure Sour Cherry Anthocyanin Extract on Cytokine-Induced Inflammatory Caco-2 Monolayers

doi: 10.3390/nu10070861

Figure Lengend Snippet: GPx enzyme activity in Caco-2 cells. Caco-2 cells were treated for 24 h with culture medium (C), pro-inflammatory cytokines (CK; 50 ng/mL TNF-α and 25 ng/mL IL-1β), or pre-treated with 50 µM anthocyanins before CK treatment (AC + CK). AC pre-treatment significantly reduced the cytokine-induced GPx activity in the cells. Data are presented as means ± SDs, n = 3. *** p

Article Snippet: Determination of Glutathione Peroxidase (GPx) Activity The glutathione peroxidase (GPx) activity of Caco-2 cells was determined with a Glutathione Peroxidase Assay Kit (Cayman Chemical, Ann Arbor, MI, USA).

Techniques: Activity Assay