performance liquid chromatography Search Results


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  • 99
    Millipore performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore liquid chromatography grade
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Liquid Chromatography Grade, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Transgenomic performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Transgenomic, used in various techniques. Bioz Stars score: 92/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    PerkinElmer performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 705 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ARKRAY performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by ARKRAY, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Tosoh Corporation performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 93/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Shimadzu Corporation performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 97/100, based on 488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Waters Corporation performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ARUP Laboratories performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by ARUP Laboratories, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Ribobio performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Ribobio, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Hitachi Ltd performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 93/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    METTLER TOLEDO performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Bio-Rad performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Hewlett-Packard performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Hewlett-Packard, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    JASCO Inc performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 91/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beckman Coulter performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Genox performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Genox, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Eurofins performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Eurofins, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Trinity Biotech performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Gilson Inc performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Metabion International AG performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nycomed performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Nycomed, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pi Proteomics performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Pi Proteomics, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    INCSTAR Corporation performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by INCSTAR Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Quest Diagnostics performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by Quest Diagnostics, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    JEOL performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by JEOL, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare performance liquid chromatography
    Intestinal permeability alteration. Stomach gavaged <t>FD40</t> was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using <t>HPLC</t> method. *P
    Performance Liquid Chromatography, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Intestinal permeability alteration. Stomach gavaged FD40 was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using HPLC method. *P

    Journal: International Journal of Clinical and Experimental Medicine

    Article Title: Sodium butyrate protects the intestinal barrier function in peritonitic mice

    doi:

    Figure Lengend Snippet: Intestinal permeability alteration. Stomach gavaged FD40 was used as the indicator for the permeability of gut mucosa. 4 h after stomach gavage, FD40 concentrations of blood plasma were measured using HPLC method. *P

    Article Snippet: Plasma FD40 was measured by high performance liquid chromatography (HPLC) using fluorescence detection model SIL-20A/SIL-20AC prominence liquid chromatograph (Waters-Millipore) (SHIMADZU Company, Japan).

    Techniques: Permeability, High Performance Liquid Chromatography

    Aromatic amino-acid levels in plasma of wild-type (WT) and phenylketonuria (PKU) female mice. Plasma samples, obtained from untreated WT and PKU mice and pegvaliase-treated PKU mice, were analyzed by high-performance liquid chromatography (HPLC) separation. Plasma samples from untreated PKU mice show markedly increased levels in plasma phenylalanine (Phe) relative to WT mice, along with a 40–50% decrease in plasma tyrosine. Plasma tryptophan levels remained similar between the two genotypes. Pegvaliase treatment decreased plasma Phe to physiological or below normal physiological levels according to dose regimen. Tyrosine and tryptophan levels in untreated and pegvaliase-treated PKU mice were similar.

    Journal: Pediatric Research

    Article Title: Maternal phenylketonuria syndrome: studies in mice suggest a potential approach to a continuing problem

    doi: 10.1038/pr.2017.323

    Figure Lengend Snippet: Aromatic amino-acid levels in plasma of wild-type (WT) and phenylketonuria (PKU) female mice. Plasma samples, obtained from untreated WT and PKU mice and pegvaliase-treated PKU mice, were analyzed by high-performance liquid chromatography (HPLC) separation. Plasma samples from untreated PKU mice show markedly increased levels in plasma phenylalanine (Phe) relative to WT mice, along with a 40–50% decrease in plasma tyrosine. Plasma tryptophan levels remained similar between the two genotypes. Pegvaliase treatment decreased plasma Phe to physiological or below normal physiological levels according to dose regimen. Tyrosine and tryptophan levels in untreated and pegvaliase-treated PKU mice were similar.

    Article Snippet: Amino-Acid Analysis Individual amino acids and standards (Sigma), and high-performance liquid chromatography reagents (Fisher), were obtained from Sigma Chemical (St Louis, MO) and Fisher Scientific (Atlanta, GA).

    Techniques: Mouse Assay, High Performance Liquid Chromatography

    Mean plasma peptide concentrations over time. The plasma concentrations of LL-37, Tα1, and LTA a in vitro were quantified by high-performance liquid chromatography. Data are means ± SEMs of three biological replicates.

    Journal: International Journal of Molecular Sciences

    Article Title: Development of a Highly Efficient Hybrid Peptide That Increases Immunomodulatory Activity Via the TLR4-Mediated Nuclear Factor-κB Signaling Pathway

    doi: 10.3390/ijms20246161

    Figure Lengend Snippet: Mean plasma peptide concentrations over time. The plasma concentrations of LL-37, Tα1, and LTA a in vitro were quantified by high-performance liquid chromatography. Data are means ± SEMs of three biological replicates.

    Article Snippet: The purity of the peptides was determined to be greater than 95% by reverse-phase high-performance liquid chromatography (Sigma-Aldrich, Singapore) and mass spectrometry (MALDI-TOF MS, Model Autoflex, Bruker Daltonics Inc., Billerica, MA, USA) ( ).

    Techniques: In Vitro, High Performance Liquid Chromatography

    Drug concentrations in hair samples with their physical characteristics calculated by high-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS) in both aliquots kept in the dark or irradiated in the solar simulator. -: absent at limit of detection (LOD, 0.005 ng/mg); (−) observed % increase.

    Journal: Brain Sciences

    Article Title: A Study on Photostability of Amphetamines and Ketamine in Hair Irradiated under Artificial Sunlight

    doi: 10.3390/brainsci8060096

    Figure Lengend Snippet: Drug concentrations in hair samples with their physical characteristics calculated by high-performance liquid chromatography high-resolution mass spectrometry (HPLC-HRMS) in both aliquots kept in the dark or irradiated in the solar simulator. -: absent at limit of detection (LOD, 0.005 ng/mg); (−) observed % increase.

    Article Snippet: High-performance liquid chromatography water was prepared using a Milli-Q Plus (Millipore, Molsheim, France) system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Irradiation

    Ultra high-performance liquid-chromatography-diode-array (UHPLC-DAD) chromatograms detected at 440 nm of a saffron extract obtained with the column Kinetex C18 at ( A ) t g = 10 min, T = 25 °C and ( B ) φ = 0.60 mL/min and t g = 10 min, T = 25 °C and φ = 1.00 mL/min. Peak assignments are reported in Table 1 .

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: UHPLC Analysis of Saffron (Crocus sativus L.): Optimization of Separation Using Chemometrics and Detection of Minor Crocetin Esters

    doi: 10.3390/molecules23081851

    Figure Lengend Snippet: Ultra high-performance liquid-chromatography-diode-array (UHPLC-DAD) chromatograms detected at 440 nm of a saffron extract obtained with the column Kinetex C18 at ( A ) t g = 10 min, T = 25 °C and ( B ) φ = 0.60 mL/min and t g = 10 min, T = 25 °C and φ = 1.00 mL/min. Peak assignments are reported in Table 1 .

    Article Snippet: High performance liquid chromatography-grade methanol and acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: High Performance Liquid Chromatography

    Effects of VME and VMA on hepatic AMPK expression and HPLC chromatogram. a AMPKα1 and AMPKα2 mRNA expression and b AMPK and ACC phosphorylation. c HPLC-PDA chromatograms of two standards mixture, VME, and VMA at 330 nm. Esculetin and schaftoside appeared at retention times of approximately 12.3 and 21.7 min, respectively. Data are presented as mean ± SEM ( n = 4); # p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Anti-obesity activity, acute toxicity, and chemical constituents of aqueous and ethanol Viola mandshurica extracts

    doi: 10.1186/s12906-017-1810-4

    Figure Lengend Snippet: Effects of VME and VMA on hepatic AMPK expression and HPLC chromatogram. a AMPKα1 and AMPKα2 mRNA expression and b AMPK and ACC phosphorylation. c HPLC-PDA chromatograms of two standards mixture, VME, and VMA at 330 nm. Esculetin and schaftoside appeared at retention times of approximately 12.3 and 21.7 min, respectively. Data are presented as mean ± SEM ( n = 4); # p

    Article Snippet: High performance liquid chromatography (HPLC) analysis of VME and VMA The standard compounds esculetin (6,7-dihydroxycoumarin, purity 98%) and schaftoside (6-C-beta-D-Glucosyl-8-C-alpha-L-arabinosylapigenin, purity 98%) were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Expressing, High Performance Liquid Chromatography

    Comparison of insulin B chain cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.

    Journal: PLoS ONE

    Article Title: Active Site Mutations Change the Cleavage Specificity of Neprilysin

    doi: 10.1371/journal.pone.0032343

    Figure Lengend Snippet: Comparison of insulin B chain cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC profile of insulin B chain cleaved by NEP and NEP mutants at 30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of insulin B chain from Table 2 . Reactions were carried out at 37°C with 15 µM insulin B chain in 20 mM MES, pH 6.5.

    Article Snippet: HPLC assays Cleavage of physiological peptides was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of the purified NEP or its mutants with 15 µM insulin B chain (Sigma Aldrich), 24 µM Aß1–40 (Anaspec), or 64 µM leu-ENK (Sigma) in 100 µL of 20 mM MES, pH 6.5, at 37°C.

    Techniques: High Performance Liquid Chromatography, Mutagenesis

    Comparison of Aß 1–40 cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC cleavage profile of Aß 1–40 cleavage by NEP and NEP mutants at ∼30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of Aß 1–40 from Table 2 . Reactions were carried out at 37°C with 15 µM Aß 1–40 in 20 mM MES, pH 6.5.

    Journal: PLoS ONE

    Article Title: Active Site Mutations Change the Cleavage Specificity of Neprilysin

    doi: 10.1371/journal.pone.0032343

    Figure Lengend Snippet: Comparison of Aß 1–40 cleavage between NEP, NEP F563L , and NEP S546E . A. HPLC cleavage profile of Aß 1–40 cleavage by NEP and NEP mutants at ∼30% hydrolysis. B. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP S546E . C. Rates of peak accumulation at each cleavage site normalized to that of NEP for mutant NEP F563L . Dotted lines indicate the overall rate of hydrolysis of Aß 1–40 from Table 2 . Reactions were carried out at 37°C with 15 µM Aß 1–40 in 20 mM MES, pH 6.5.

    Article Snippet: HPLC assays Cleavage of physiological peptides was measured via reverse phase high performance liquid chromatography (HPLC) following incubation of the purified NEP or its mutants with 15 µM insulin B chain (Sigma Aldrich), 24 µM Aß1–40 (Anaspec), or 64 µM leu-ENK (Sigma) in 100 µL of 20 mM MES, pH 6.5, at 37°C.

    Techniques: High Performance Liquid Chromatography, Mutagenesis