Journal: BMC Complementary and Alternative Medicine
Article Title: Anti-obesity activity, acute toxicity, and chemical constituents of aqueous and ethanol Viola mandshurica extracts
Figure Lengend Snippet: Effects of VME and VMA on hepatic AMPK expression and HPLC chromatogram. a AMPKα1 and AMPKα2 mRNA expression and b AMPK and ACC phosphorylation. c HPLC-PDA chromatograms of two standards mixture, VME, and VMA at 330 nm. Esculetin and schaftoside appeared at retention times of approximately 12.3 and 21.7 min, respectively. Data are presented as mean ± SEM ( n = 4); # p
Article Snippet: High performance liquid chromatography (HPLC) analysis of VME and VMA The standard compounds esculetin (6,7-dihydroxycoumarin, purity 98%) and schaftoside (6-C-beta-D-Glucosyl-8-C-alpha-L-arabinosylapigenin, purity 98%) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Techniques: Expressing, High Performance Liquid Chromatography