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  • 99
    Thermo Fisher perfect transfection kit
    Genetic inhibition of Bax prevents FFA-induced lysosomal permeabilization. Bax expression in HepG2 cells was silenced by small interfering-RNA (siRNA) as described in Materials and Methods. After <t>transfection</t> of HepG2 cells with Bax siRNA, an immunoblot for Bax and β-actin was performed ( A ). Following 48 h of transfection with the Bax siRNA constructs, cells were loaded with LysoTracker Red (LTR) for 30 min at 37°C and incubated with 0.2 mM palmitate in media with BSA for 24 h. B : cells were then imaged by confocal microscopy. C : cells were scored as either having a predominantly punctuate or diffuse fluorescent pattern. Bax inhibition significantly reduced palmitate-initiated lysosomal permeabilization (* P
    Perfect Transfection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perfect transfection kit/product/Thermo Fisher
    Average 99 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    perfect transfection kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Thermo Fisher perfect lipid transfection kit
    Stable <t>transfection</t> of skin fibroblasts with TSP2 expression vectors. Immortalized TSP2-null dermal fibroblasts were transfected with mTSP2 expression vectors and Lipofectin. Cells transfected with sense pZeoSVmTSP2 expressed TSP2 protein as revealed by immunocytochemistry with anti-TSP2 antibodies (left), whereas cells transfected with antisense vector, pZeoSV2PSTm, were negative (right). Bar, 100 μm.
    Perfect Lipid Transfection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/perfect lipid transfection kit/product/Thermo Fisher
    Average 85 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    perfect lipid transfection kit - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Genetic inhibition of Bax prevents FFA-induced lysosomal permeabilization. Bax expression in HepG2 cells was silenced by small interfering-RNA (siRNA) as described in Materials and Methods. After transfection of HepG2 cells with Bax siRNA, an immunoblot for Bax and β-actin was performed ( A ). Following 48 h of transfection with the Bax siRNA constructs, cells were loaded with LysoTracker Red (LTR) for 30 min at 37°C and incubated with 0.2 mM palmitate in media with BSA for 24 h. B : cells were then imaged by confocal microscopy. C : cells were scored as either having a predominantly punctuate or diffuse fluorescent pattern. Bax inhibition significantly reduced palmitate-initiated lysosomal permeabilization (* P

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: Bax inhibition protects against free fatty acid-induced lysosomal permeabilization

    doi: 10.1152/ajpgi.00509.2005

    Figure Lengend Snippet: Genetic inhibition of Bax prevents FFA-induced lysosomal permeabilization. Bax expression in HepG2 cells was silenced by small interfering-RNA (siRNA) as described in Materials and Methods. After transfection of HepG2 cells with Bax siRNA, an immunoblot for Bax and β-actin was performed ( A ). Following 48 h of transfection with the Bax siRNA constructs, cells were loaded with LysoTracker Red (LTR) for 30 min at 37°C and incubated with 0.2 mM palmitate in media with BSA for 24 h. B : cells were then imaged by confocal microscopy. C : cells were scored as either having a predominantly punctuate or diffuse fluorescent pattern. Bax inhibition significantly reduced palmitate-initiated lysosomal permeabilization (* P

    Article Snippet: Serum-free medium containing a 1:9 ratio of DNA-single cationic lipid (Perfect transfection kit; Invitrogen) was used, and cells were incubated in the mixture for 8 h. Next, transfected cells were cultured in serum-supplemented medium for 36 h. After recovery from transfection, cells were grown in selective medium containing 200 μg/ml of hygromycin B (Gibco, Gaithersburg, MD).

    Techniques: Inhibition, Expressing, Small Interfering RNA, Transfection, Construct, Incubation, Confocal Microscopy

    Bax inhibition reduces palmitate-induced apoptosis. Bax expression in HepG2 cells was silenced by siRNA as described in Materials and Methods. After transfection of HepG2 cells with Bax siRNA, cells were incubated with 0.2 mM palmitate in media with BSA for 24 h. A : apoptosis was quantified by using the DNA-binding dye 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) and fluorescence microscopy. Bax inhibition significantly reduced palmitate-associated liver cell apoptosis (* P

    Journal: American journal of physiology. Gastrointestinal and liver physiology

    Article Title: Bax inhibition protects against free fatty acid-induced lysosomal permeabilization

    doi: 10.1152/ajpgi.00509.2005

    Figure Lengend Snippet: Bax inhibition reduces palmitate-induced apoptosis. Bax expression in HepG2 cells was silenced by siRNA as described in Materials and Methods. After transfection of HepG2 cells with Bax siRNA, cells were incubated with 0.2 mM palmitate in media with BSA for 24 h. A : apoptosis was quantified by using the DNA-binding dye 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) and fluorescence microscopy. Bax inhibition significantly reduced palmitate-associated liver cell apoptosis (* P

    Article Snippet: Serum-free medium containing a 1:9 ratio of DNA-single cationic lipid (Perfect transfection kit; Invitrogen) was used, and cells were incubated in the mixture for 8 h. Next, transfected cells were cultured in serum-supplemented medium for 36 h. After recovery from transfection, cells were grown in selective medium containing 200 μg/ml of hygromycin B (Gibco, Gaithersburg, MD).

    Techniques: Inhibition, Expressing, Transfection, Incubation, Binding Assay, Fluorescence, Microscopy

    Stable transfection of skin fibroblasts with TSP2 expression vectors. Immortalized TSP2-null dermal fibroblasts were transfected with mTSP2 expression vectors and Lipofectin. Cells transfected with sense pZeoSVmTSP2 expressed TSP2 protein as revealed by immunocytochemistry with anti-TSP2 antibodies (left), whereas cells transfected with antisense vector, pZeoSV2PSTm, were negative (right). Bar, 100 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Matricellular Proteins as Modulators of Cell-Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2

    doi:

    Figure Lengend Snippet: Stable transfection of skin fibroblasts with TSP2 expression vectors. Immortalized TSP2-null dermal fibroblasts were transfected with mTSP2 expression vectors and Lipofectin. Cells transfected with sense pZeoSVmTSP2 expressed TSP2 protein as revealed by immunocytochemistry with anti-TSP2 antibodies (left), whereas cells transfected with antisense vector, pZeoSV2PSTm, were negative (right). Bar, 100 μm.

    Article Snippet: Cells were transfected by Lipofectin with the PerFect lipid transfection kit (Invitrogen), and were grown in the presence of Zeocin, 0.2 mg/ml, to obtain stably transfected cell populations.

    Techniques: Stable Transfection, Expressing, Transfection, Immunocytochemistry, Plasmid Preparation

    Rescue of the adhesive defect and restoration of normal MMP2 activity in TSP2-null dermal fibroblasts by transfection with mTSP2 cDNA. Immortalized dermal fibroblasts derived from TSP2-null mice were transfected with sense and antisense mTSP2 cDNA and compared for attachment to fibronectin (FN)-coated and noncoated (NC) tissue culture surfaces. (A) Compared with wild-type cells, nontransfected immortalized TSP2-null fibroblasts showed a marked defect in adhesion to tissue culture plastic, and coating with FN did not completely correct the adhesive defect. (B) Adhesive defect of TSP2-null fibroblasts was corrected by transfection with sense mTSP2 cDNA (S), but not with antisense mTSP2 cDNA (AS) expression vectors (P

    Journal: Molecular Biology of the Cell

    Article Title: Matricellular Proteins as Modulators of Cell-Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2

    doi:

    Figure Lengend Snippet: Rescue of the adhesive defect and restoration of normal MMP2 activity in TSP2-null dermal fibroblasts by transfection with mTSP2 cDNA. Immortalized dermal fibroblasts derived from TSP2-null mice were transfected with sense and antisense mTSP2 cDNA and compared for attachment to fibronectin (FN)-coated and noncoated (NC) tissue culture surfaces. (A) Compared with wild-type cells, nontransfected immortalized TSP2-null fibroblasts showed a marked defect in adhesion to tissue culture plastic, and coating with FN did not completely correct the adhesive defect. (B) Adhesive defect of TSP2-null fibroblasts was corrected by transfection with sense mTSP2 cDNA (S), but not with antisense mTSP2 cDNA (AS) expression vectors (P

    Article Snippet: Cells were transfected by Lipofectin with the PerFect lipid transfection kit (Invitrogen), and were grown in the presence of Zeocin, 0.2 mg/ml, to obtain stably transfected cell populations.

    Techniques: Activity Assay, Transfection, Derivative Assay, Mouse Assay, Expressing

    Inhibition of NK function by transient transfection with dominant-negative MAPK/ERK2 in YT effector cells. Equal aliquots of YT cells were left untransfected or transiently transfected with KD MAPK/ERK2 in which K52R substitution was constructed, or with mutant ERK2 where Thr and Tyr residues in the TEY motif were replaced with glutamic acid (TEYE) or with alanine and phenylalanine (TAYF). The wild-type ERK2 plasmid ( WT ) was also used to transfect YT cells. After 24 h at 37°C, untransfected and transfected YT cells were assessed for viability and adjusted to the appropriate concentrations of viable cells before testing for lysis of 51 Cr-labeled Raji tumor cells at the indicated E/T ratios. The SEM of each mean percent cytotoxicity was

    Journal: The Journal of Experimental Medicine

    Article Title: Control of Lytic Function by Mitogen-activated Protein Kinase/Extracellular Regulatory Kinase 2 (ERK2) in a Human Natural Killer Cell Line: Identification of Perforin and Granzyme B Mobilization by Functional ERK2

    doi:

    Figure Lengend Snippet: Inhibition of NK function by transient transfection with dominant-negative MAPK/ERK2 in YT effector cells. Equal aliquots of YT cells were left untransfected or transiently transfected with KD MAPK/ERK2 in which K52R substitution was constructed, or with mutant ERK2 where Thr and Tyr residues in the TEY motif were replaced with glutamic acid (TEYE) or with alanine and phenylalanine (TAYF). The wild-type ERK2 plasmid ( WT ) was also used to transfect YT cells. After 24 h at 37°C, untransfected and transfected YT cells were assessed for viability and adjusted to the appropriate concentrations of viable cells before testing for lysis of 51 Cr-labeled Raji tumor cells at the indicated E/T ratios. The SEM of each mean percent cytotoxicity was

    Article Snippet: YT cells were transfected using the PerFect Lipids transfection kit (Invitrogen Corp., San Diego, CA), and transfection efficiency in YT cells was first optimized using the control plasmid pcDNA3.1/ His/lacZ (GAL) included in the kit.

    Techniques: Inhibition, Transfection, Dominant Negative Mutation, Construct, Mutagenesis, Plasmid Preparation, Lysis, Labeling