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  • 99
    Thermo Fisher 5 amino 1 pentanol
    5 Amino 1 Pentanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore 1 pentanol
    1 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore 4 methyl 2 pentanol
    4 Methyl 2 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher 1 pentanol
    1 Pentanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore 5 amino 1 pentanol
    5 Amino 1 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore pentanol
    Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore 2 pentanol
    2 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore 3 pentanol
    3 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore 4 methyl 1 pentanol
    4 Methyl 1 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Merck KGaA 3 pentanol
    3 Pentanol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore 4 metyl 2 pentanol
    Results of the computational modeling of the tetrahedral intermediates of ( R )- (A) and ( S <t>)-4-methyl-4-phenyl-2-pentanol</t> (B). It is apparent that only the S -enantiomer side chain comfortably fits into the substrate binding pocket (residues shown in green).
    4 Metyl 2 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Cayman Chemical win 18 446
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    Win 18 446, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore 2 2 3 3 4 4 5 5 octafluoro 1 pentanol
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    2 2 3 3 4 4 5 5 Octafluoro 1 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck KGaA 2 pentanol
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    2 Pentanol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore 3 methyl 1 pentanol
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    3 Methyl 1 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore 4 4 dimethyl 2 pentanol
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    4 4 Dimethyl 2 Pentanol, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore 4 cyano 4
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    4 Cyano 4, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Tokyo Chemical Industry 5 amino 1 pentanol ap
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    5 Amino 1 Pentanol Ap, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 5 amino 1 pentanol s5
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    5 Amino 1 Pentanol S5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 5 amino pentanol
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    5 Amino Pentanol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co 4 methyl 2 pentanol
    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p
    4 Methyl 2 Pentanol, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Results of the computational modeling of the tetrahedral intermediates of ( R )- (A) and ( S )-4-methyl-4-phenyl-2-pentanol (B). It is apparent that only the S -enantiomer side chain comfortably fits into the substrate binding pocket (residues shown in green).

    Journal: Tetrahedron

    Article Title: Enantioselective Transesterification Catalysis by Nanosized Serine Protease Subtilisin Carlsberg Particles in Tetrahydrofuran

    doi: 10.1016/j.tet.2010.01.053

    Figure Lengend Snippet: Results of the computational modeling of the tetrahedral intermediates of ( R )- (A) and ( S )-4-methyl-4-phenyl-2-pentanol (B). It is apparent that only the S -enantiomer side chain comfortably fits into the substrate binding pocket (residues shown in green).

    Article Snippet: Subtilisin Carlsberg (SC), MβCD, 2-butanol ( 1 ), 2-pentanol ( 2 ), 2-hexanol ( 3 ), 2-heptanol ( 4 ), 2-octanol ( 5 ), 3-methyl-2-butanol ( 6 ), 4-methyl-2-pentanol ( 7 ), 5-methyl-2-hexanol ( 8 ), 6-methyl-2-heptanol ( 9 ), 4-phenyl-2-butanol ( 10 ), 4,4-dimethyl-2-pentanol ( 11 ), 4-methyl-4-phenyl-2-pentanol ( 12 ), 1-phenyl-1-propanol ( 13 ), and 1-p-tolyl-1-propanol ( 14 ) were from Sigma-Aldrich (St. Louis, MO).

    Techniques: Binding Assay

    Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A)  Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment).  (C)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment)  (E)  Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD +  and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment).  (B, D, F)  IC 50  and EC 50  values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p

    Journal: Bioorganic & medicinal chemistry

    Article Title: Design, Synthesis, and Ex Vivo Evaluation of a Selective Inhibitor for Retinaldehyde Dehydrogenase Enzymes.

    doi: 10.1016/j.bmc.2018.10.009

    Figure Lengend Snippet: Effect of DAR and WIN on RALDH2 Activity in Control and Recovering Choroids. (A) Inhibition of ATRA synthesis in control and recovering choroidal lysates following 20 min pre-incubation with DAR (0.01 – 6 μM) or vehicle (DMSO). After pre-incubation, the enzyme reaction was initiated by addition of NAD + and RAL (25 μM) and allowed to proceed for 30 min at 37°C. Data are representative of results from two experiments (n = 3 – 5/experiment). (C) Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by DAR. Control and recovering choroids were placed in organ culture and treated with DAR (0.01 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD + and RAL (25 μM) (n = 9 −14 choroids/concentration). Data represent results of three independent experiments (n = 3 – 5 choroids/experiment) (E) Inhibition of RALDH2 activity in living choroid tissue, “ ex vivo ” by WIN 18,446 (WIN). Control and recovering choroids were placed in organ culture and treated with WIN (0.1 – 10 μM) or vehicle (DMSO) for 24 hrs at 37°C and 5% CO 2 . Following isolation of cytosol fractions, the enzyme reaction was initiated by addition of NAD + and RAL (25 μM) (n = 6 choroids/concentration). ATRA was quantified using HPLC. Data represent results of two independent experiments (n = 3/choroids/experiment). (B, D, F) IC 50 and EC 50 values for DARand WIN were calculated by non-linear regression analyses of data from recovering choroids from figures A, C, and E, respectively. * p

    Article Snippet: For tissue incubated with WIN 18,446 (WIN; Cayman Chemical Company, Ann Arbor, MI), WIN was serially diluted (0.01 – 5 μM) in N2 media (initial dilution: 1:10,000 in N2 medium from a 10 mM stock in DMSO).

    Techniques: Activity Assay, Inhibition, Incubation, Ex Vivo, Organ Culture, Isolation, Concentration Assay, High Performance Liquid Chromatography