peltier thermal cycler 200 Search Results


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  • 89
    MJ Research peltier thermal cycler 200
    Peltier Thermal Cycler 200, supplied by MJ Research, used in various techniques. Bioz Stars score: 89/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier thermal cycler 200/product/MJ Research
    Average 89 stars, based on 227 article reviews
    Price from $9.99 to $1999.99
    peltier thermal cycler 200 - by Bioz Stars, 2020-07
    89/100 stars
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    89
    Biozym peltier thermal cycler 200
    Peltier Thermal Cycler 200, supplied by Biozym, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier thermal cycler 200/product/Biozym
    Average 89 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    peltier thermal cycler 200 - by Bioz Stars, 2020-07
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    89
    Bio-Rad peltier thermal cycler ptc 200
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Peltier Thermal Cycler Ptc 200, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier thermal cycler ptc 200/product/Bio-Rad
    Average 89 stars, based on 150 article reviews
    Price from $9.99 to $1999.99
    peltier thermal cycler ptc 200 - by Bioz Stars, 2020-07
    89/100 stars
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    85
    Bio-Rad peltier thermal cyclers ptc 200
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Peltier Thermal Cyclers Ptc 200, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier thermal cyclers ptc 200/product/Bio-Rad
    Average 85 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    peltier thermal cyclers ptc 200 - by Bioz Stars, 2020-07
    85/100 stars
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    89
    Biozym peltier thermal cycler ptc 200
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Peltier Thermal Cycler Ptc 200, supplied by Biozym, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier thermal cycler ptc 200/product/Biozym
    Average 89 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    peltier thermal cycler ptc 200 - by Bioz Stars, 2020-07
    89/100 stars
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    88
    MJ Research peltier ptc 200 thermal cycler
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Peltier Ptc 200 Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 88/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier ptc 200 thermal cycler/product/MJ Research
    Average 88 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    peltier ptc 200 thermal cycler - by Bioz Stars, 2020-07
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    89
    Roche peltier thermal cycler ptc 200
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Peltier Thermal Cycler Ptc 200, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    peltier thermal cycler ptc 200 - by Bioz Stars, 2020-07
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    92
    GeneWorks ptc 200 peltier thermal cycler
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Ptc 200 Peltier Thermal Cycler, supplied by GeneWorks, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    MJ Research peltier model ptc 200 thermal cycler
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Peltier Model Ptc 200 Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 85/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peltier model ptc 200 thermal cycler/product/MJ Research
    Average 85 stars, based on 61 article reviews
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    peltier model ptc 200 thermal cycler - by Bioz Stars, 2020-07
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    85
    MJ Research ptc 200 peltier thermal gradient cycler
    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research <t>PTC-200</t> <t>Peltier</t> Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum
    Ptc 200 Peltier Thermal Gradient Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptc 200 peltier thermal gradient cycler/product/MJ Research
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ptc 200 peltier thermal gradient cycler - by Bioz Stars, 2020-07
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    Image Search Results


    Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum

    Journal: Pharmacognosy Magazine

    Article Title: Specific PCR Identification between Peucedanum praeruptorum and Angelica decursiva and Identification between Them and Adulterant Using DNA Barcode

    doi: 10.4103/0973-1296.197658

    Figure Lengend Snippet: Optimization of different influence factors in specific PCR of A. decursiva . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using A. decursiva identification primer pairs ZHQH-CP3s\ZHQH-CP3a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B. Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 144, 72, 36, 18, 9, 4.5 and 2.25 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates A. decursiva , and 2 indicates P. praeruptorum . F . PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates A. decursiva , and 2 indicates P. praeruptorum

    Article Snippet: Instruments and reagents PCR instrument: MJ Research PTC-200 Peltier Thermal Cycler (Bio-Rad company), Tprofessional standard Thermocycler (Biometra company) and Light Cycler® 96 System (Roche company); Mikro 120 type microcentrifuge (Hettich company); BG-Power 600k electrophoresis apparatus (Beijing Baijing Biotechnology Co., Ltd.); automatic gel imaging analyzer (Beijing Baijing biotechnology limited company); UV-2102 PCS type ultraviolet visible spectrophotometer [Unique (Shanghai) Instrument Co. Ltd.]; WH-3 type vortex oscillator (Shanghai Huxi analytical instrument Factory Co. Ltd.); SYQ-DSX-280B type portable stainless steel autoclave (Shanghai Shenan medical instrument factory).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Negative Control

    Optimization of different influence factors in specific PCR of P. praeruptorum . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using P. praeruptorum identification primer pairs QH-CP19s\QH-CP19a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B . Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of P. praeruptorum with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates P. praeruptorum , and 2 indicates A. decursiva . F. PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates P. praeruptorum , and 2 indicates A. decursiva .

    Journal: Pharmacognosy Magazine

    Article Title: Specific PCR Identification between Peucedanum praeruptorum and Angelica decursiva and Identification between Them and Adulterant Using DNA Barcode

    doi: 10.4103/0973-1296.197658

    Figure Lengend Snippet: Optimization of different influence factors in specific PCR of P. praeruptorum . M: DNA Marker (containing a mix of 6 individual DNA fragments from top to bottom denoting 600, 500, 400, 300, 200, and 100 bp). A Amplification results using P. praeruptorum identification primer pairs QH-CP19s\QH-CP19a: 1, 2, and 3 indicate A. decursiva ; 4, 5, and 6 indicate P. praeruptorum ; 7 indicates negative control without DNA template. B . Annealing temperatures: numbers from 1 to 21 indicate annealing temperatures of P. praeruptorum with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C; numbers from 22 to 42 indicate annealing temperatures of A. decursiva with 48, 49, 51, 50, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67 and 68 °C. C. Cycle numbers: numbers from 1 to 4 indicate cycle numbers of P. praeruptorum with 20, 25, 30, and 35 cycles; numbers from 5 to 8 indicate cycle numbers of A. decursiva with 20, 25, 30, and 35 cycles. D. DNA template dosages: numbers from 1 to 7 indicate DNA template dosages of P. praeruptorum with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng; numbers from 8 to 14 indicate DNA template dosages of A. decursiva with 42, 21, 10.5, 5.25, 2.625, 1.313, and 0.657 ng. E. Taq enzyme species: a indicates Taq DNA Polymerase, b indicates Taq Plus DNA Polymerase, c indicates Hot Start Taq DNA Polymerase, 1 indicates P. praeruptorum , and 2 indicates A. decursiva . F. PCR instruments: a indicates LightCycler ®96 System (Roche company), b indicates Professional standard Thermocycler (Biometra company), c indicates MJ research PTC-200 Peltier Thermal Cycler (Bio-Rad company), 1 indicates P. praeruptorum , and 2 indicates A. decursiva .

    Article Snippet: Instruments and reagents PCR instrument: MJ Research PTC-200 Peltier Thermal Cycler (Bio-Rad company), Tprofessional standard Thermocycler (Biometra company) and Light Cycler® 96 System (Roche company); Mikro 120 type microcentrifuge (Hettich company); BG-Power 600k electrophoresis apparatus (Beijing Baijing Biotechnology Co., Ltd.); automatic gel imaging analyzer (Beijing Baijing biotechnology limited company); UV-2102 PCS type ultraviolet visible spectrophotometer [Unique (Shanghai) Instrument Co. Ltd.]; WH-3 type vortex oscillator (Shanghai Huxi analytical instrument Factory Co. Ltd.); SYQ-DSX-280B type portable stainless steel autoclave (Shanghai Shenan medical instrument factory).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Negative Control