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  • 85
    Johnson & Johnson pe1
    Pe1, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe1/product/Johnson & Johnson
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pe1 - by Bioz Stars, 2020-07
    85/100 stars
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    88
    Illumina Inc illumina pe1
    Illumina Pe1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina pe1/product/Illumina Inc
    Average 88 stars, based on 107 article reviews
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    illumina pe1 - by Bioz Stars, 2020-07
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    89
    Illumina Inc pe1 0
    Pe1 0, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe1 0/product/Illumina Inc
    Average 89 stars, based on 195 article reviews
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    pe1 0 - by Bioz Stars, 2020-07
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    92
    Millipore pe1 e2sumo1
    Pe1 E2sumo1, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pe1 e2sumo1 - by Bioz Stars, 2020-07
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    90
    TransGen biotech co peasy blunt e1 vector pe1
    Peasy Blunt E1 Vector Pe1, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peasy blunt e1 vector pe1/product/TransGen biotech co
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    91
    Illumina Inc pe1 0 primer
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Pe1 0 Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe1 0 primer/product/Illumina Inc
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    91
    Eurofins pe1 54 apoe3
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Pe1 54 Apoe3, supplied by Eurofins, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pe1 54 apoe3 - by Bioz Stars, 2020-07
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    90
    Illumina Inc illumina adaptors pe1
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Illumina Adaptors Pe1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina adaptors pe1/product/Illumina Inc
    Average 90 stars, based on 5 article reviews
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    illumina adaptors pe1 - by Bioz Stars, 2020-07
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    91
    Bioo Scientific primer pe1 pe2
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Primer Pe1 Pe2, supplied by Bioo Scientific, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    primer pe1 pe2 - by Bioz Stars, 2020-07
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    85
    Illumina Inc standard illumina pe1
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Standard Illumina Pe1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard illumina pe1/product/Illumina Inc
    Average 85 stars, based on 6 article reviews
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    standard illumina pe1 - by Bioz Stars, 2020-07
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    91
    Illumina Inc illumina pe1 primer
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Illumina Pe1 Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina pe1 primer/product/Illumina Inc
    Average 91 stars, based on 8 article reviews
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    illumina pe1 primer - by Bioz Stars, 2020-07
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    85
    Illumina Inc pe1 pcr primer
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Pe1 Pcr Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe1 pcr primer/product/Illumina Inc
    Average 85 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    pe1 pcr primer - by Bioz Stars, 2020-07
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    85
    Thermo Fisher pe1 pcr primer
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Pe1 Pcr Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Illumina Inc complementary illumina adapters pe1
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Complementary Illumina Adapters Pe1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complementary illumina adapters pe1/product/Illumina Inc
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    89
    Illumina Inc truseq pe1 sequence
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Truseq Pe1 Sequence, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq pe1 sequence/product/Illumina Inc
    Average 89 stars, based on 45 article reviews
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    truseq pe1 sequence - by Bioz Stars, 2020-07
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    85
    Olympus standard olympus enteroscope pe1
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Standard Olympus Enteroscope Pe1, supplied by Olympus, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/standard olympus enteroscope pe1/product/Olympus
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    standard olympus enteroscope pe1 - by Bioz Stars, 2020-07
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    85
    Solexa pe primers pe1 0
    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded <t>Illumina</t> adapters and Illumina PE1.0 primer sequence (yellow).
    Pe Primers Pe1 0, supplied by Solexa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pe primers pe1 0/product/Solexa
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    pe primers pe1 0 - by Bioz Stars, 2020-07
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    Image Search Results


    The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded Illumina adapters and Illumina PE1.0 primer sequence (yellow).

    Journal: bioRxiv

    Article Title: IMPLICON: an ultra-deep sequencing method to uncover DNA methylation at imprinted regions

    doi: 10.1101/2020.03.21.000042

    Figure Lengend Snippet: The IMPLICON method A. Schematic view of the murine karyotype depicting the location of the regions detected by IMPLICON; black arrowheads – control regions; red arrowheads – imprinted regions. B. Schematic representation of the mouse Dlk1-Dio3 imprinted cluster; Mat – Maternal inherited chromosome; Pat – Paternal inherited chromosome; ICR – imprinting control region; red arrows – primers to amplify Dlk1-Dio3 ICR; genomic region is not drawn to scale. C. Brief scheme of the IMPLICON method and its approximate timeline; bisulfite conversion of genomic DNA converts unmethylated cytosines to uracils (letters in yellow), whilst methylated cytosines are retained as cytosines (in white); two rounds of PCR are then performed: the 1 st PCR amplifies each region for each sample separately and adds 8 random nucleotides (N 8 ) for data deduplication and adapter sequences; after pooling amplicons for each biological sample, a 2 nd PCR completes a sequence-ready library with sample-barcodes for multiplexing; white lollipops – unmethylated CpGs; black lollipops – methylated CpGs; black DNA strand – targeted strand for amplification; light grey DNA strand – strand not targeted for amplification; Dark grey and green boxes - primers annealing the targeted strand (dark grey), containing adapter sequences (green); green and yellow - primers annealing the adapters (green), containing barcoded Illumina adapters and Illumina PE1.0 primer sequence (yellow).

    Article Snippet: The 20μl PCR pool was amplified using 1μl of 10μM Illumina PE1.0 primer (same for all samples), 1μl of 10μM Illumina iTAG primer (distinct for each sample) and 25μl 2x KAPA HiFi Uracil+ ReadyMix in a 50μl reaction using the following conditions: initial denaturation at 98°C for 45 seconds, 5 cycles of 98°C denaturation for 15 seconds, 65°C annealing for 30 seconds and extension at 72°C for 30 seconds; followed by a final extension at 72°C for 5 minutes.

    Techniques: Methylation, Polymerase Chain Reaction, Sequencing, Multiplexing, Amplification