Journal: Journal of phycology

Article Title: Phylogenetic analysis of LSU and SSU rDNA group I introns of lichen photobionts associated with the genera Xanthoria and Xanthomendoza (Teloschistaceae, lichenized Ascomycetes)

doi: 10.1111/jpy.12126

Figure Lengend Snippet: DNA extraction, PCR amplification and sequencing

Article Snippet: The PCR reactions were performed in 50 μL reaction volumes containing a reaction mix of 0.2 mM of each of four dNTPs, 1.5 μM of each PCR primer, and 1.25 U of Taq DNA polymerase (Sigma Aldrich, Buchs, SG, Switzerland) and 5 μL of 10X PCR buffer (100 mM Tris-HCl, pH 8.3, 500 mM KCl, 15 mM MgCl2 and 0.01% gelatin).

Techniques: DNA Extraction, Polymerase Chain Reaction, Amplification, Sequencing

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

Journal: Data in Brief

Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

doi: 10.1016/j.dib.2016.02.035

Figure Lengend Snippet: Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

Techniques: Nested PCR, Polymerase Chain Reaction, Marker, Negative Control

Journal: Journal of Pest Science

Article Title: Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids

doi: 10.1007/s10340-015-0685-8

Figure Lengend Snippet: Gel image of PCR products amplified with the three customised multiplex PCR assays and visualised with the QIAxcel system. MPI ( left side ) comprises group/family-specific primers for seven taxa: beetles/thrips, spiders, aphids, earthworms, springtails, dipterans, and lacewings. MPII spiders ( upper right side ) covers two spider families, i.e. lycosids and linyphiids, as well as the genus Pachygnatha . MPII beetles/thrips ( lower right side ) addresses six taxa: the carabid genera Poecilus , Bembidion , Pterostichus, and Harpalus , the ladybeetle Coccinella septempunctata as well as thrips ( Frankliniella , Limothrips ). The shortest and longest fragments within each lane represent the two alignment markers (AM; 15 and 3000 bp) as indicated in the left panel . For amplicon lengths see Fig. 1 and Table 3 . Mixes of DNA templates of targets; approximately 1000 ds copies each in PCR

Article Snippet: The PCR protocol of the MPII beetles/thrips assay differed only slightly from the MPII spiders : 1.5 µl of DNA extract and 30 mM TMAC (Sigma-Aldrich) were used in the total volume of 10 µl (plus PCR-grade water to adjust the volume); thermocycling conditions as described above, but with an annealing temperature of 63.5 °C.

Techniques: Polymerase Chain Reaction, Amplification, Multiplex Assay