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  • 99
    Thermo Fisher topo pcr4 ta cloning vector
    Topo Pcr4 Ta Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr4 ta topo vector
    Pcr4 Ta Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr4 topo ta
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta/product/Thermo Fisher
    Average 92 stars, based on 856 article reviews
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    pcr4 topo ta - by Bioz Stars, 2020-08
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    99
    Thermo Fisher pcr4 topo ta vectors
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta vectors/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    pcr4 topo ta vectors - by Bioz Stars, 2020-08
    99/100 stars
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    89
    Thermo Fisher pcr4 topo ta vector
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta vector/product/Thermo Fisher
    Average 89 stars, based on 734 article reviews
    Price from $9.99 to $1999.99
    pcr4 topo ta vector - by Bioz Stars, 2020-08
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    89
    Thermo Fisher pcr4 topo ta plasmids
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta plasmids/product/Thermo Fisher
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    Thermo Fisher intermediate vector pcr4 topo ta
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Intermediate Vector Pcr4 Topo Ta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Stratagene pcr4 topo ta
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta/product/Stratagene
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr4 topo ta - by Bioz Stars, 2020-08
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    85
    Thermo Fisher pcr4 topo ta cloning vector
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta cloning vector/product/Thermo Fisher
    Average 85 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    pcr4 topo ta cloning vector - by Bioz Stars, 2020-08
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    89
    Thermo Fisher pcr4 topo ta sequencing vector
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topo Ta Sequencing Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topo ta sequencing vector/product/Thermo Fisher
    Average 89 stars, based on 149 article reviews
    Price from $9.99 to $1999.99
    pcr4 topo ta sequencing vector - by Bioz Stars, 2020-08
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    90
    Thermo Fisher pcr 4 topo ta vector
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr 4 Topo Ta Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 4 topo ta vector/product/Thermo Fisher
    Average 90 stars, based on 423 article reviews
    Price from $9.99 to $1999.99
    pcr 4 topo ta vector - by Bioz Stars, 2020-08
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    85
    Thermo Fisher pcr4 topota vector
    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into <t>pCR4-TOPO</t> TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.
    Pcr4 Topota Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr4 topota vector/product/Thermo Fisher
    Average 85 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr4 topota vector - by Bioz Stars, 2020-08
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    Image Search Results


    Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into pCR4-TOPO TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.

    Journal: The Journal of Biological Chemistry

    Article Title: Modeling the Etiology of p53-mutated Cancer Cells *

    doi: 10.1074/jbc.M116.724781

    Figure Lengend Snippet: Bisulfite sequencing of the TP53 promoter in heterozygote Mutant p53/WT silenced clones. A, genomic DNA was isolated from heterozygote clones and modified by bisulfite. The TP53 promoter was amplified by nested PCR and cloned into pCR4-TOPO TA cloning vector. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine. No methylated cytosines were detected in the TP53 promoter from heterozygote clones. B, genomic DNA was isolated from the Mutant p53/WT silenced clones and modified by bisulfite. TP53 promoter was amplified by nested PCR and cloned as described above. 9–10 colonies were picked and sequenced. Open circles represent non-methylated cytosine, and solid circles represent methylated cytosine.

    Article Snippet: The amplified DNA fragment was cloned into the pCR4-TOPO TA cloning vector (Life Technologies, Inc.), and 9–10 individual clones for each cell line were sequenced.

    Techniques: Methylation Sequencing, Mutagenesis, Clone Assay, Isolation, Modification, Amplification, Nested PCR, TA Cloning, Plasmid Preparation, Methylation