pcr-product Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher exosap it pcr product cleanup
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Exosap It Pcr Product Cleanup, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap it pcr product cleanup/product/Thermo Fisher
    Average 99 stars, based on 467 article reviews
    Price from $9.99 to $1999.99
    exosap it pcr product cleanup - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore high pure pcr product purification kit
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    High Pure Pcr Product Purification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high pure pcr product purification kit/product/Millipore
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    high pure pcr product purification kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr product
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Pcr Product, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1768 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product/product/Thermo Fisher
    Average 99 stars, based on 1768 article reviews
    Price from $9.99 to $1999.99
    pcr product - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    93
    MiniPCR pcr product analysis
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Pcr Product Analysis, supplied by MiniPCR, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product analysis/product/MiniPCR
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr product analysis - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Promega pcr product purification
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Pcr Product Purification, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification/product/Promega
    Average 93 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    pcr product purification - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    94
    MACHEREY NAGEL pcr product purification
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Pcr Product Purification, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification/product/MACHEREY NAGEL
    Average 94 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    pcr product purification - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr product cleanup reagent
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Pcr Product Cleanup Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product cleanup reagent/product/Thermo Fisher
    Average 99 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    pcr product cleanup reagent - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher pcr product kit
    Nested <t>RT-PCR</t> products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with <t>ethidium</t> bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions
    Pcr Product Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product kit/product/Thermo Fisher
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    pcr product kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    86
    Stratagene pcr product cloning
    Plasmid constructs used for expression studies. The entire bla KPC gene with its flanking regions was amplified by <t>PCR</t> from K. pneumoniae KN2303 (Tn 4401b ), K. pneumoniae YC (Tn 4401a ), and K. pneumoniae 475 (Tn 4401c ) and cloned into the <t>PCR-ScriptCam</t> vector,
    Pcr Product Cloning, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product cloning/product/Stratagene
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pcr product cloning - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    96
    Promega pcr product purification columns
    Plasmid constructs used for expression studies. The entire bla KPC gene with its flanking regions was amplified by <t>PCR</t> from K. pneumoniae KN2303 (Tn 4401b ), K. pneumoniae YC (Tn 4401a ), and K. pneumoniae 475 (Tn 4401c ) and cloned into the <t>PCR-ScriptCam</t> vector,
    Pcr Product Purification Columns, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification columns/product/Promega
    Average 96 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pcr product purification columns - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    94
    Bioneer Corporation pcr product purification kit
    Cloned ( A ) and expressed ( B,C ) Coh 03501 gene. Lane 1: <t>PCR</t> product. Lane 2: non-recombinant pET26b. Lane 3: recombinant pET26b. Lane 4: digested pET26b with Nde I and Not I. Lane 5: colony PCR, Lane 6: <t>DNA</t> ladder, Lane 7: protein molecular mass marker. Lane 8: purified rSAM (protein concentration: 0.4 mg/ml), Lane 9: total cellular expressed protein from E. coli BL21 (DE3) (protein concentration: 1.6 mg/ml). Lane 10: a single band from the western blotting analysis against the rSAM His-tag sequence. Lane 11: protein molecular mass marker.
    Pcr Product Purification Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Bioneer Corporation
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    90
    Promega pcr product purification kit
    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 <t>PCR</t> for MKPV <t>DNA</t> using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).
    Pcr Product Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Promega
    Average 90 stars, based on 69 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    93
    Roche pcr product purification kit
    Relative expression of genes identified from the <t>SSH</t> library, MYD88 isoforms- and autophagy-related genes in esophageal cancer tissues, assessed using real-time <t>PCR.</t> (a) Relative expression levels of genes that are overexpressed in esophageal tumor (FOXO3, GAPDH, and MYD88 variants). Expression of MYD88v4 was not detectable in samples. (b) Detection of autophagy in esophageal tumors. Relative expression of autophagy-related genes (Beclin1, ATG12, Gabarapl1, LC3, and PIK3C3) in esophageal tumor tissues. (●) represent the mean and whiskers the SEM of expression in samples ( n = 10). * P
    Pcr Product Purification Kit, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Roche
    Average 93 stars, based on 282 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    95
    Roche pcr product purifications kits
    Relative expression of genes identified from the <t>SSH</t> library, MYD88 isoforms- and autophagy-related genes in esophageal cancer tissues, assessed using real-time <t>PCR.</t> (a) Relative expression levels of genes that are overexpressed in esophageal tumor (FOXO3, GAPDH, and MYD88 variants). Expression of MYD88v4 was not detectable in samples. (b) Detection of autophagy in esophageal tumors. Relative expression of autophagy-related genes (Beclin1, ATG12, Gabarapl1, LC3, and PIK3C3) in esophageal tumor tissues. (●) represent the mean and whiskers the SEM of expression in samples ( n = 10). * P
    Pcr Product Purifications Kits, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purifications kits/product/Roche
    Average 95 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    pcr product purifications kits - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    99
    Thermo Fisher exo sap it express pcr product cleanup
    Relative expression of genes identified from the <t>SSH</t> library, MYD88 isoforms- and autophagy-related genes in esophageal cancer tissues, assessed using real-time <t>PCR.</t> (a) Relative expression levels of genes that are overexpressed in esophageal tumor (FOXO3, GAPDH, and MYD88 variants). Expression of MYD88v4 was not detectable in samples. (b) Detection of autophagy in esophageal tumors. Relative expression of autophagy-related genes (Beclin1, ATG12, Gabarapl1, LC3, and PIK3C3) in esophageal tumor tissues. (●) represent the mean and whiskers the SEM of expression in samples ( n = 10). * P
    Exo Sap It Express Pcr Product Cleanup, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exo sap it express pcr product cleanup/product/Thermo Fisher
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    exo sap it express pcr product cleanup - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Axygen pcr product purification kit
    Relative expression of genes identified from the <t>SSH</t> library, MYD88 isoforms- and autophagy-related genes in esophageal cancer tissues, assessed using real-time <t>PCR.</t> (a) Relative expression levels of genes that are overexpressed in esophageal tumor (FOXO3, GAPDH, and MYD88 variants). Expression of MYD88v4 was not detectable in samples. (b) Detection of autophagy in esophageal tumors. Relative expression of autophagy-related genes (Beclin1, ATG12, Gabarapl1, LC3, and PIK3C3) in esophageal tumor tissues. (●) represent the mean and whiskers the SEM of expression in samples ( n = 10). * P
    Pcr Product Purification Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Axygen
    Average 94 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    94
    Sangon Biotech pcr product purification kit
    Identification of PerR regulon in S. <t>suis.</t> (A) Relative expression levels of genes dpr , metQ , relA , pmtA and sodA in strain Δ perR compared to its parental strain SC-19. Relative abundance of the transcripts was determined by real-time <t>RT-PCR</t> from the total RNAs derived from strains Δ perR and SC-19 in mid-log phase. gapdh was used as the internal control. (B) Different concentration of PerR proteins binds to dpr and metQIN promoters (500 bp and 300 bp respectively), gidA promoter (300 bp) was used as the negative control. (C) The structure of the dpr and metQIN promoters. -10 and −35 regions of the promoters are shown by the boxes. The start codon is labeled by blod fonts. The predicted PerR-box is underlined.
    Pcr Product Purification Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Sangon Biotech
    Average 94 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    87
    Beijing SBS Genetech pcr product purification kits
    Identification of PerR regulon in S. <t>suis.</t> (A) Relative expression levels of genes dpr , metQ , relA , pmtA and sodA in strain Δ perR compared to its parental strain SC-19. Relative abundance of the transcripts was determined by real-time <t>RT-PCR</t> from the total RNAs derived from strains Δ perR and SC-19 in mid-log phase. gapdh was used as the internal control. (B) Different concentration of PerR proteins binds to dpr and metQIN promoters (500 bp and 300 bp respectively), gidA promoter (300 bp) was used as the negative control. (C) The structure of the dpr and metQIN promoters. -10 and −35 regions of the promoters are shown by the boxes. The start codon is labeled by blod fonts. The predicted PerR-box is underlined.
    Pcr Product Purification Kits, supplied by Beijing SBS Genetech, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kits/product/Beijing SBS Genetech
    Average 87 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kits - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    86
    Boehringer Mannheim pcr product purification kit
    Identification of PerR regulon in S. <t>suis.</t> (A) Relative expression levels of genes dpr , metQ , relA , pmtA and sodA in strain Δ perR compared to its parental strain SC-19. Relative abundance of the transcripts was determined by real-time <t>RT-PCR</t> from the total RNAs derived from strains Δ perR and SC-19 in mid-log phase. gapdh was used as the internal control. (B) Different concentration of PerR proteins binds to dpr and metQIN promoters (500 bp and 300 bp respectively), gidA promoter (300 bp) was used as the negative control. (C) The structure of the dpr and metQIN promoters. -10 and −35 regions of the promoters are shown by the boxes. The start codon is labeled by blod fonts. The predicted PerR-box is underlined.
    Pcr Product Purification Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Boehringer Mannheim
    Average 86 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    93
    Roche pcr product recovery kit
    Detection of large-scale deletions of <t>mtDNA</t> from human washed sperm by long-range <t>PCR</t> method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.
    Pcr Product Recovery Kit, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product recovery kit/product/Roche
    Average 93 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    pcr product recovery kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    General Bioscience Inc pcr product purification kit
    Detection of large-scale deletions of <t>mtDNA</t> from human washed sperm by long-range <t>PCR</t> method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.
    Pcr Product Purification Kit, supplied by General Bioscience Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/General Bioscience Inc
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    86
    Stratagene pcr product purification kit
    Detection of large-scale deletions of <t>mtDNA</t> from human washed sperm by long-range <t>PCR</t> method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.
    Pcr Product Purification Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Stratagene
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    85
    tiangen biotech co pcr product purification kit
    Detection of large-scale deletions of <t>mtDNA</t> from human washed sperm by long-range <t>PCR</t> method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.
    Pcr Product Purification Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/tiangen biotech co
    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    86
    Genomed GmbH pcr product purification kit
    Detection of large-scale deletions of <t>mtDNA</t> from human washed sperm by long-range <t>PCR</t> method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.
    Pcr Product Purification Kit, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 86/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Genomed GmbH
    Average 86 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    87
    Macrogen pcr product purification kit
    Detection of large-scale deletions of <t>mtDNA</t> from human washed sperm by long-range <t>PCR</t> method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.
    Pcr Product Purification Kit, supplied by Macrogen, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Macrogen
    Average 87 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    89
    Shanghai Generay Biotech pcr product purification kit
    2.5% agarose gel electrophoreses for the three round <t>PCR</t> products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by <t>T4</t> and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.
    Pcr Product Purification Kit, supplied by Shanghai Generay Biotech, used in various techniques. Bioz Stars score: 89/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Shanghai Generay Biotech
    Average 89 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    92
    Genomed GmbH pcr product sequencing
    2.5% agarose gel electrophoreses for the three round <t>PCR</t> products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by <t>T4</t> and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.
    Pcr Product Sequencing, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product sequencing/product/Genomed GmbH
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr product sequencing - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    88
    Bio Basic Canada pcr product purification kit
    2.5% agarose gel electrophoreses for the three round <t>PCR</t> products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by <t>T4</t> and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.
    Pcr Product Purification Kit, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Bio Basic Canada
    Average 88 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    91
    Biocolor Ltd pcr product purification kit
    2.5% agarose gel electrophoreses for the three round <t>PCR</t> products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by <t>T4</t> and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.
    Pcr Product Purification Kit, supplied by Biocolor Ltd, used in various techniques. Bioz Stars score: 91/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/Biocolor Ltd
    Average 91 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    92
    TaKaRa pcr product purification kit
    2.5% agarose gel electrophoreses for the three round <t>PCR</t> products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by <t>T4</t> and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.
    Pcr Product Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr product purification kit/product/TaKaRa
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    pcr product purification kit - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Nested RT-PCR products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with ethidium bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions

    Journal: Medical Oncology (Northwood, London, England)

    Article Title: Gene expression profiling of peripheral blood cells: new insights into Ewing sarcoma biology and clinical applications

    doi: 10.1007/s12032-014-0109-2

    Figure Lengend Snippet: Nested RT-PCR products obtained from the control frozen tumor tissues and PB specimens of ES patients visualized by electrophoresis in 2 % agarose gel stained with ethidium bromide. a The second round of amplification was performed using one-fifth of the first round PCR product. b The second round of amplification was performed using one-fifth of the 10× dilution of the first round PCR product. M: 1-kb Plus DNA ladder (Invitrogen). Lanes 1–5 PCR products from frozen tumor tissues; lanes 6–11 PCR products from selected PB specimens from ES patients; lanes 12, 13 PCR negative controls for two rounds of amplification; lane 14 positive control, EWS – FLI1 type 2 fusion (RD-ES cell line). Lanes 2, 3, 5, and 10 EWS – FLI1 type 1 fusions, lanes 1 and 4 EWS – FLI1 type 2 fusions

    Article Snippet: PCR products were visualized by electrophoresis in 2 % TBE agarose gels stained with ethidium bromide, purified using ExoSAP-IT, PCR Product Clean-Up (Affymetrix, Santa Clara, CA, USA) and prepared for direct sequencing using BigDye® Terminator version 3.1 Cycle Sequencing Kit (Life Technologies) with subsequent precipitation using ExTerminator kit (A & A Biotechnology, Gdynia, Poland).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Electrophoresis, Agarose Gel Electrophoresis, Staining, Amplification, Polymerase Chain Reaction, Positive Control

    Plasmid constructs used for expression studies. The entire bla KPC gene with its flanking regions was amplified by PCR from K. pneumoniae KN2303 (Tn 4401b ), K. pneumoniae YC (Tn 4401a ), and K. pneumoniae 475 (Tn 4401c ) and cloned into the PCR-ScriptCam vector,

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Role of ISKpn7 and Deletions in blaKPC Gene Expression

    doi: 10.1128/AAC.00334-12

    Figure Lengend Snippet: Plasmid constructs used for expression studies. The entire bla KPC gene with its flanking regions was amplified by PCR from K. pneumoniae KN2303 (Tn 4401b ), K. pneumoniae YC (Tn 4401a ), and K. pneumoniae 475 (Tn 4401c ) and cloned into the PCR-ScriptCam vector,

    Article Snippet: The PCR-ScriptCam vector was used for PCR product cloning (Stratagene, Massy, France).

    Techniques: Plasmid Preparation, Construct, Expressing, Amplification, Polymerase Chain Reaction, Clone Assay

    Cloned ( A ) and expressed ( B,C ) Coh 03501 gene. Lane 1: PCR product. Lane 2: non-recombinant pET26b. Lane 3: recombinant pET26b. Lane 4: digested pET26b with Nde I and Not I. Lane 5: colony PCR, Lane 6: DNA ladder, Lane 7: protein molecular mass marker. Lane 8: purified rSAM (protein concentration: 0.4 mg/ml), Lane 9: total cellular expressed protein from E. coli BL21 (DE3) (protein concentration: 1.6 mg/ml). Lane 10: a single band from the western blotting analysis against the rSAM His-tag sequence. Lane 11: protein molecular mass marker.

    Journal: Scientific Reports

    Article Title: Novel halo- and thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and biochemical characterization

    doi: 10.1038/s41598-019-55587-9

    Figure Lengend Snippet: Cloned ( A ) and expressed ( B,C ) Coh 03501 gene. Lane 1: PCR product. Lane 2: non-recombinant pET26b. Lane 3: recombinant pET26b. Lane 4: digested pET26b with Nde I and Not I. Lane 5: colony PCR, Lane 6: DNA ladder, Lane 7: protein molecular mass marker. Lane 8: purified rSAM (protein concentration: 0.4 mg/ml), Lane 9: total cellular expressed protein from E. coli BL21 (DE3) (protein concentration: 1.6 mg/ml). Lane 10: a single band from the western blotting analysis against the rSAM His-tag sequence. Lane 11: protein molecular mass marker.

    Article Snippet: DNA extraction kit was purchased from Peqlab and PCR product purification kit was obtained from BioNEER (Seoul, Korea).

    Techniques: Clone Assay, Polymerase Chain Reaction, Recombinant, Marker, Purification, Protein Concentration, Western Blot, Sequencing

    Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 PCR for MKPV DNA using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).

    Journal: PLoS Pathogens

    Article Title: Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys

    doi: 10.1371/journal.ppat.1008262

    Figure Lengend Snippet: Haematoxylin/eosin (H E)-staining of historical formalin-fixed paraffin-embedded (FFPE) mouse kidney necropsy samples from the University of North Carolina, Chapel Hill, USA and Israel, paired with agarose gels of 25 cycle 869–870 PCR for MKPV DNA using DNA extracted from FFPE kidney shavings of necropsies from the same sites. For the Israel specimen, ISH for MKPV nucleic acids was also performed. Arrows show examples of inclusion bodies in each H E stain. PCR panels include size markers at left (0.1–1.0 kb, in 0.1 kb increments) and control DNA at right from MKPV-infected Centenary Institute Rag1 –/– mice (+ve) or MKPV-free Rag1 –/– mice (-ve, sourced from Australian BioResources, Mittagong NSW).

    Article Snippet: The DNA was extracted from the excised agarose using a PCR product purification kit (Promega, Madison, Wisconsin, USA), following the manufacturer’s instructions for agarose-embedded DNA, then Sanger-sequenced (Macrogen, Seoul, S Korea) using the appropriate MKPV-specific primer. qPCR for MKPV DNA was performed exactly as described before [ ], using primers 869 and 870, an annealing temperature of 48˚C and an extension time of 0.5 min. For RNA qPCR, RNA equivalent to 1 ug tissue was pre-treated with DNase, as described above, then reverse-transcribed using oligo-dT primer and SuperScript III reverse transcriptase (ThermoFisher, Carlsbad, CA, USA) at 50 ˚C for 60 min. First-strand cDNA equivalent to 40 ng tissue was then used as template for qPCR reactions using primers 947 and 948, iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), an annealing temperature of 68.5˚C and an extension time of 0.5 min.

    Techniques: Staining, Formalin-fixed Paraffin-Embedded, Polymerase Chain Reaction, In Situ Hybridization, Infection, Mouse Assay

    Map of the MKPV genome. (A-B) Maps of the MKPV/MuCPV strains from Centenary Institute (CI, accession MH670587), Memorial Sloan Kettering Cancer Center (MSKCC, accession MH670588) and New York City basements (wild-NY, MF175078). “Bowties” indicate terminal repeats (TR). (A) Single nucleotide variations (SNV) between the CI, MSKCC and wild-NY accessions. Vertical lines—differences between accessions. Half height vertical lines—polymorphisms within an accession. ▼; 2 bp insertion in the CI strain. ▲; 1 bp insertion in a CI sub-strain. Dashed lines—missing extremities in MSKCC and wild-NY accessions, which consist of the exterior inverted repeats in the full-length CI sequence. (B-C) Alternative splicing allows production of the polypeptides p10, p15, NS1, NS2, NP and VP. Black, brown or blue shading indicate the relative reading frames of ORFs. p15, p10 and NP could theoretically be produced from multiple transcripts. Orange or red indicate peptides present in LC-MS/MS datasets PXD014938 (this paper) or PXD010540 [ 9 ], respectively. Exon or intron sequences flanking splice sites are shown in black or red text, respectively. (C) Quantitation of spliced MKPV reads in RNAseq data pooled from two MKPV-infected kidneys. Columns indicate splice site usage (left y-axis); heights of arcs (right y-axis) indicate the abundance of specific splice combinations. See S4 Table for more information. (D-E) Detection of spliced transcripts by RT-dependent PCR, using primers mapped in A-B. Input templates were MKPV-infected (D) kidney DNA or (E) DNAse/ExoI-treated kidney RNA, converted (+RT) or mock-converted (-RT) to cDNA. RT-PCR products corresponding to transcripts 1 to 4 are indicated by white numbers. (F) Mapping of transcription start and stop sites by RACE. See S1 Fig for RACE details. Major 5’ and 3’ RACE products, indicated by black arrows and corresponding to transcripts 2 to 4 or polyadenylation signals A and B, were gel-purified and Sanger sequenced. Other RACE products mentioned in the text are indicated by white arrows.

    Journal: PLoS Pathogens

    Article Title: Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys

    doi: 10.1371/journal.ppat.1008262

    Figure Lengend Snippet: Map of the MKPV genome. (A-B) Maps of the MKPV/MuCPV strains from Centenary Institute (CI, accession MH670587), Memorial Sloan Kettering Cancer Center (MSKCC, accession MH670588) and New York City basements (wild-NY, MF175078). “Bowties” indicate terminal repeats (TR). (A) Single nucleotide variations (SNV) between the CI, MSKCC and wild-NY accessions. Vertical lines—differences between accessions. Half height vertical lines—polymorphisms within an accession. ▼; 2 bp insertion in the CI strain. ▲; 1 bp insertion in a CI sub-strain. Dashed lines—missing extremities in MSKCC and wild-NY accessions, which consist of the exterior inverted repeats in the full-length CI sequence. (B-C) Alternative splicing allows production of the polypeptides p10, p15, NS1, NS2, NP and VP. Black, brown or blue shading indicate the relative reading frames of ORFs. p15, p10 and NP could theoretically be produced from multiple transcripts. Orange or red indicate peptides present in LC-MS/MS datasets PXD014938 (this paper) or PXD010540 [ 9 ], respectively. Exon or intron sequences flanking splice sites are shown in black or red text, respectively. (C) Quantitation of spliced MKPV reads in RNAseq data pooled from two MKPV-infected kidneys. Columns indicate splice site usage (left y-axis); heights of arcs (right y-axis) indicate the abundance of specific splice combinations. See S4 Table for more information. (D-E) Detection of spliced transcripts by RT-dependent PCR, using primers mapped in A-B. Input templates were MKPV-infected (D) kidney DNA or (E) DNAse/ExoI-treated kidney RNA, converted (+RT) or mock-converted (-RT) to cDNA. RT-PCR products corresponding to transcripts 1 to 4 are indicated by white numbers. (F) Mapping of transcription start and stop sites by RACE. See S1 Fig for RACE details. Major 5’ and 3’ RACE products, indicated by black arrows and corresponding to transcripts 2 to 4 or polyadenylation signals A and B, were gel-purified and Sanger sequenced. Other RACE products mentioned in the text are indicated by white arrows.

    Article Snippet: The DNA was extracted from the excised agarose using a PCR product purification kit (Promega, Madison, Wisconsin, USA), following the manufacturer’s instructions for agarose-embedded DNA, then Sanger-sequenced (Macrogen, Seoul, S Korea) using the appropriate MKPV-specific primer. qPCR for MKPV DNA was performed exactly as described before [ ], using primers 869 and 870, an annealing temperature of 48˚C and an extension time of 0.5 min. For RNA qPCR, RNA equivalent to 1 ug tissue was pre-treated with DNase, as described above, then reverse-transcribed using oligo-dT primer and SuperScript III reverse transcriptase (ThermoFisher, Carlsbad, CA, USA) at 50 ˚C for 60 min. First-strand cDNA equivalent to 40 ng tissue was then used as template for qPCR reactions using primers 947 and 948, iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA), an annealing temperature of 68.5˚C and an extension time of 0.5 min.

    Techniques: Sequencing, Produced, Liquid Chromatography with Mass Spectroscopy, Quantitation Assay, Infection, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Purification

    Relative expression of genes identified from the SSH library, MYD88 isoforms- and autophagy-related genes in esophageal cancer tissues, assessed using real-time PCR. (a) Relative expression levels of genes that are overexpressed in esophageal tumor (FOXO3, GAPDH, and MYD88 variants). Expression of MYD88v4 was not detectable in samples. (b) Detection of autophagy in esophageal tumors. Relative expression of autophagy-related genes (Beclin1, ATG12, Gabarapl1, LC3, and PIK3C3) in esophageal tumor tissues. (●) represent the mean and whiskers the SEM of expression in samples ( n = 10). * P

    Journal: Gastroenterology Research and Practice

    Article Title: Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy

    doi: 10.1155/2014/185035

    Figure Lengend Snippet: Relative expression of genes identified from the SSH library, MYD88 isoforms- and autophagy-related genes in esophageal cancer tissues, assessed using real-time PCR. (a) Relative expression levels of genes that are overexpressed in esophageal tumor (FOXO3, GAPDH, and MYD88 variants). Expression of MYD88v4 was not detectable in samples. (b) Detection of autophagy in esophageal tumors. Relative expression of autophagy-related genes (Beclin1, ATG12, Gabarapl1, LC3, and PIK3C3) in esophageal tumor tissues. (●) represent the mean and whiskers the SEM of expression in samples ( n = 10). * P

    Article Snippet: Cloning and Similarity Searches The constructed SSH libraries were purified using the PCR Product Purification Kit (Roche Applied Science), and the purified PCR products were ligated into the pUC19 cloning vector and transformed into Escherichia coli NovaBlue competent cells (Novagen, Madison, WI, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Identification of PerR regulon in S. suis. (A) Relative expression levels of genes dpr , metQ , relA , pmtA and sodA in strain Δ perR compared to its parental strain SC-19. Relative abundance of the transcripts was determined by real-time RT-PCR from the total RNAs derived from strains Δ perR and SC-19 in mid-log phase. gapdh was used as the internal control. (B) Different concentration of PerR proteins binds to dpr and metQIN promoters (500 bp and 300 bp respectively), gidA promoter (300 bp) was used as the negative control. (C) The structure of the dpr and metQIN promoters. -10 and −35 regions of the promoters are shown by the boxes. The start codon is labeled by blod fonts. The predicted PerR-box is underlined.

    Journal: BMC Microbiology

    Article Title: A Fur-like protein PerR regulates two oxidative stress response related operons dpr and metQIN in Streptococcus suis

    doi: 10.1186/1471-2180-12-85

    Figure Lengend Snippet: Identification of PerR regulon in S. suis. (A) Relative expression levels of genes dpr , metQ , relA , pmtA and sodA in strain Δ perR compared to its parental strain SC-19. Relative abundance of the transcripts was determined by real-time RT-PCR from the total RNAs derived from strains Δ perR and SC-19 in mid-log phase. gapdh was used as the internal control. (B) Different concentration of PerR proteins binds to dpr and metQIN promoters (500 bp and 300 bp respectively), gidA promoter (300 bp) was used as the negative control. (C) The structure of the dpr and metQIN promoters. -10 and −35 regions of the promoters are shown by the boxes. The start codon is labeled by blod fonts. The predicted PerR-box is underlined.

    Article Snippet: The DNA fragments of the candidate promoters were amplified from S. suis SC-19 genomic DNA and purified by using the PCR Product Purification Kit (Sangon Biotech, Shanghai, China).

    Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Concentration Assay, Negative Control, Labeling

    Detection of large-scale deletions of mtDNA from human washed sperm by long-range PCR method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.

    Journal: International Journal of Fertility & Sterility

    Article Title: A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran

    doi:

    Figure Lengend Snippet: Detection of large-scale deletions of mtDNA from human washed sperm by long-range PCR method. A: The 5860 bp band represents the PCR product of normal mtDNA with primer pair LF3-HR4. Lane M is the 1-kb DNA size. B: Using the primer sets LF3-HR4, the 5860 bp band was amplified from the wild-type mtDNA, the 994 bp and 883 bands were amplified from the 4866 bp and 4977 bp-deleted mtDNA, respectively. Spermatozoa in lanes 1-4 had the motility scores of 5.0, 20.0, 30.0, 40.0% respectively. Lane 5 is the blank, in which the sperm DNA was omitted from the reaction mixture. Lane M is the1-kb DNA size marker. C. The arrow indicates the band of 994 bp produced with primer pair LF3-HR4. Using a short extension time of 1 minute at 72˚C, the longer DNA product from wild-type mtDNA could not be produced and only mtDNA with 4866 bp-deletion was amplified. Lanes N and A normal and abnormal groups, respectively.

    Article Snippet: DNA sequencing The PCR product (534 bp mtDNA fragment) amplified from the 4866 bp deleted mtDNA using the LF4- HR3 primer pair was purified with the PCR product recovery kit (Roche Applied Science, Mannheim, Germany).

    Techniques: Polymerase Chain Reaction, Amplification, Marker, Produced

    Detection of the 4866 and 4977 bp-deleted mtDNA by the primer shift PCR method in human spermatozoa. Lanes 1-2 represent the PCR products of 423 and 793 bp amplified from the 4977 bp-deleted mtDNA with primer pair LF4-HR3 and LF4-HR4, respectively. Lanes 3-4 represent the PCR products of 534 and 904 bp amplified from the 4866 bpdeleted mtDNA with primer pairs LF4-HR3 and LF4-HR4 respectively. Lane M indicates the 1-kb DNA size marker.

    Journal: International Journal of Fertility & Sterility

    Article Title: A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran

    doi:

    Figure Lengend Snippet: Detection of the 4866 and 4977 bp-deleted mtDNA by the primer shift PCR method in human spermatozoa. Lanes 1-2 represent the PCR products of 423 and 793 bp amplified from the 4977 bp-deleted mtDNA with primer pair LF4-HR3 and LF4-HR4, respectively. Lanes 3-4 represent the PCR products of 534 and 904 bp amplified from the 4866 bpdeleted mtDNA with primer pairs LF4-HR3 and LF4-HR4 respectively. Lane M indicates the 1-kb DNA size marker.

    Article Snippet: DNA sequencing The PCR product (534 bp mtDNA fragment) amplified from the 4866 bp deleted mtDNA using the LF4- HR3 primer pair was purified with the PCR product recovery kit (Roche Applied Science, Mannheim, Germany).

    Techniques: Polymerase Chain Reaction, Amplification, Marker

    Semi-quantitative PCR analysis of mtDNA with the 4866 bp deletion using serial dilution method in human spermatozoa. A. Lanes 1-8 represent the PCR products amplified from total mtDNA serially diluted 2 8 , 2 9 , 2 10 , 2 11 , 2 12 , 2 13 , 2 14 , 2 15 -fold, respectively with primer pair LF2-HR2. B. Lanes 9-14 represent the PCR products amplified from 4866-bp deleted mtDNA serially diluted to 2 1 , 2 2 , 2 3 , 2 4 , 2 5 , 2 6 -fold, respectively with primer pair LF4–HR3. Lane M indicates the 1-kb DNA marker.

    Journal: International Journal of Fertility & Sterility

    Article Title: A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran

    doi:

    Figure Lengend Snippet: Semi-quantitative PCR analysis of mtDNA with the 4866 bp deletion using serial dilution method in human spermatozoa. A. Lanes 1-8 represent the PCR products amplified from total mtDNA serially diluted 2 8 , 2 9 , 2 10 , 2 11 , 2 12 , 2 13 , 2 14 , 2 15 -fold, respectively with primer pair LF2-HR2. B. Lanes 9-14 represent the PCR products amplified from 4866-bp deleted mtDNA serially diluted to 2 1 , 2 2 , 2 3 , 2 4 , 2 5 , 2 6 -fold, respectively with primer pair LF4–HR3. Lane M indicates the 1-kb DNA marker.

    Article Snippet: DNA sequencing The PCR product (534 bp mtDNA fragment) amplified from the 4866 bp deleted mtDNA using the LF4- HR3 primer pair was purified with the PCR product recovery kit (Roche Applied Science, Mannheim, Germany).

    Techniques: Real-time Polymerase Chain Reaction, Serial Dilution, Polymerase Chain Reaction, Amplification, Marker

    DNA sequence of the 534 bp PCR product was obtained by using the LF4-HR3 primer pair, indicating that 534 bp band was amplified from mtDNA with a novel 4866 bp deletion while the wild-type mtDNA yields a product of 5400 bp (8251-13650).

    Journal: International Journal of Fertility & Sterility

    Article Title: A Novel Large-Scale Deletion of The Mitochondrial DNA of Spermatozoa of Men in North Iran

    doi:

    Figure Lengend Snippet: DNA sequence of the 534 bp PCR product was obtained by using the LF4-HR3 primer pair, indicating that 534 bp band was amplified from mtDNA with a novel 4866 bp deletion while the wild-type mtDNA yields a product of 5400 bp (8251-13650).

    Article Snippet: DNA sequencing The PCR product (534 bp mtDNA fragment) amplified from the 4866 bp deleted mtDNA using the LF4- HR3 primer pair was purified with the PCR product recovery kit (Roche Applied Science, Mannheim, Germany).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification

    2.5% agarose gel electrophoreses for the three round PCR products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by T4 and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.

    Journal: PLoS ONE

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain

    doi: 10.1371/journal.pone.0039251

    Figure Lengend Snippet: 2.5% agarose gel electrophoreses for the three round PCR products. Electrophoreses were run in 1 x TAE at 60 V for 40 min. Lanes M and M2: DNA marker I and pUC19 DNA/MspI Marker, respectively; Lanes 1, 3, and 5: the first, second, and third round PCR products, respectively; Lanes 2, 4, and 6: the negative controls. ( A ) and ( B )The first round PCR templates were the ligation products of linkers A–B joined by T4 and E. coli DNA ligases, respectively. ( C ) and ( D ) The first round PCR templates were the ligation products of linkers C–D joined by T4 and E. coli DNA ligases, respectively. ( E ) The first round PCR templates were the ligation products cut from the denaturing PAGE gel.

    Article Snippet: These sequencing results indicated that: (i) about 80% (calculated according to the formula: the signal intensity from the ligation products without base deletion + the signal intensity from the ligation products with base deletion = 1, namely, 1÷1.25×% = 80%) of the ligation products purified with a PCR product purification kit did not contain these base deletions, meaning that some linkers had been correctly joined by T4 and E. coli DNA ligases; (ii) the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit; and (iii) perhaps these base deletions might be partly caused by PCR as well as by the base deletions at the 5′-ends of the linkers.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker, Ligation, Polyacrylamide Gel Electrophoresis

    DNA sequencing results of the third round PCR products. The letters on the top are the expected DNA sequences. The downward arrows and the upward arrows indicate the ligation junctions of the sense strands and the antisense strands, respectively. ( A ) and ( B ) The sequencing templates were prepared from the ligation products of linkers A–B joined by T4 and E. coli DNA ligases, respectively. The ligation products were purified by using a PCR product purification kit before PCR. There was a 1-base deletion (-G) at the ligation junctions of both sense and antisense strands. The signal intensity from these deletions was only equivalent to about 25% of that from the normal sequences. ( C ) and ( D ) The sequencing templates were prepared from the ligation products of linkers C–D by T4 and E. coli DNA ligases, respectively. The ligation products were purified by using a PCR product purification kit before PCR. A 5-base deletion (-GGAGC) was found at the ligation junction of the antisense strand. The signal intensity from the deletion was only equivalent to about 25% of that from the normal sequence. ( E ) and ( F ) DNA sequencing template was prepared from the unpurified ligation products of linkers A–B and C–D, respectively. A 1-base deletion (-G) or a 5-base deletion (-GGAGC) was found at the ligation junctions of both sense and antisense strands of linkers A–B, or the ligation junction of the antisense strand of linkers C–D, respectively. The signal intensity from these deletions was equivalent to or even stronger than that from the normal sequence. ( G ) DNA sequencing template was prepared from the ligation products of linkers A–B cut from the denaturing PAGE gel. There was a 1-base deletion (-G) at the ligation junctions of both sense and antisense strands. ( H ) DNA sequencing template was prepared from the negative control of linkers A–B cut from the denaturing PAGE gel. There was 1-base deletion (-G) at the ligation junctions of both sense and antisense strands.

    Journal: PLoS ONE

    Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain

    doi: 10.1371/journal.pone.0039251

    Figure Lengend Snippet: DNA sequencing results of the third round PCR products. The letters on the top are the expected DNA sequences. The downward arrows and the upward arrows indicate the ligation junctions of the sense strands and the antisense strands, respectively. ( A ) and ( B ) The sequencing templates were prepared from the ligation products of linkers A–B joined by T4 and E. coli DNA ligases, respectively. The ligation products were purified by using a PCR product purification kit before PCR. There was a 1-base deletion (-G) at the ligation junctions of both sense and antisense strands. The signal intensity from these deletions was only equivalent to about 25% of that from the normal sequences. ( C ) and ( D ) The sequencing templates were prepared from the ligation products of linkers C–D by T4 and E. coli DNA ligases, respectively. The ligation products were purified by using a PCR product purification kit before PCR. A 5-base deletion (-GGAGC) was found at the ligation junction of the antisense strand. The signal intensity from the deletion was only equivalent to about 25% of that from the normal sequence. ( E ) and ( F ) DNA sequencing template was prepared from the unpurified ligation products of linkers A–B and C–D, respectively. A 1-base deletion (-G) or a 5-base deletion (-GGAGC) was found at the ligation junctions of both sense and antisense strands of linkers A–B, or the ligation junction of the antisense strand of linkers C–D, respectively. The signal intensity from these deletions was equivalent to or even stronger than that from the normal sequence. ( G ) DNA sequencing template was prepared from the ligation products of linkers A–B cut from the denaturing PAGE gel. There was a 1-base deletion (-G) at the ligation junctions of both sense and antisense strands. ( H ) DNA sequencing template was prepared from the negative control of linkers A–B cut from the denaturing PAGE gel. There was 1-base deletion (-G) at the ligation junctions of both sense and antisense strands.

    Article Snippet: These sequencing results indicated that: (i) about 80% (calculated according to the formula: the signal intensity from the ligation products without base deletion + the signal intensity from the ligation products with base deletion = 1, namely, 1÷1.25×% = 80%) of the ligation products purified with a PCR product purification kit did not contain these base deletions, meaning that some linkers had been correctly joined by T4 and E. coli DNA ligases; (ii) the ligation products of the linkers phosphorylated by T4 DNA ligase could be selectively collected by the PCR product purification kit; and (iii) perhaps these base deletions might be partly caused by PCR as well as by the base deletions at the 5′-ends of the linkers.

    Techniques: DNA Sequencing, Polymerase Chain Reaction, Ligation, Sequencing, Purification, Polyacrylamide Gel Electrophoresis, Negative Control