pcr-ii topo ta vector Search Results


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  • 99
    Thermo Fisher pcr topo ii ta cloning vector
    Pcr Topo Ii Ta Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr 2 1 topo ii ta cloning vector
    Pcr 2 1 Topo Ii Ta Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher pcrii topo ta vectors
    Pcrii Topo Ta Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher pcrii topo ta vector
    Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the <t>pCRII-TOPO-TA</t> vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.
    Pcrii Topo Ta Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcrii topo ta vector/product/Thermo Fisher
    Average 90 stars, based on 402 article reviews
    Price from $9.99 to $1999.99
    pcrii topo ta vector - by Bioz Stars, 2020-07
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    99
    Thermo Fisher ta topo cloning vector pcrii
    Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the <t>pCRII-TOPO-TA</t> vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.
    Ta Topo Cloning Vector Pcrii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo ta dual promoter pcrii vector
    Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the <t>pCRII-TOPO-TA</t> vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.
    Topo Ta Dual Promoter Pcrii Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher pcr ii topo ta vector
    Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the <t>pCRII-TOPO-TA</t> vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.
    Pcr Ii Topo Ta Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 108 article reviews
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    pcr ii topo ta vector - by Bioz Stars, 2020-07
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    91
    Thermo Fisher topo ta sequencing vector pcrii topo
    Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the <t>pCRII-TOPO-TA</t> vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.
    Topo Ta Sequencing Vector Pcrii Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the pCRII-TOPO-TA vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: RNA Interference of mRNA Processing Factors in Drosophila S2 Cells

    doi: 10.1385/1-59259-750-5:245

    Figure Lengend Snippet: Overview of the RNAi procedure. Complementary DNAs that will be used to generate transcription templates are cloned by performing RT-PCR and cloning the resulting PCR product into the pCRII-TOPO-TA vector. These vectors are used as templates in PCR reactions with the T7 and SP6 RNA polymerase promoter primers to yield a PCR product containing the cDNA sequence flanked by T7 and SP6 RNA polymerase promoters. The transcription templates are separately transcribed with T7 and SP6 RNA polymerase to yield top- and bottom-strand RNAs. The two RNA strands are annealed and 20 μg of the RNA is incubated with S2 cells for 3 d. After incubation with the dsRNA, protein levels can be analyzed by Western blotting and changes in alternative splicing can be monitored by RT-PCR and/or RNase protection.

    Article Snippet: For this purpose, we typically use the pCRII-TOPO-TA vector (Invitrogen).

    Techniques: Clone Assay, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Plasmid Preparation, Sequencing, Incubation, Western Blot