pcr-based reactions Search Results


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  • 99
    Thermo Fisher single cell to ct qrt pcr kit
    Single Cell To Ct Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction genomic dna
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Genomic Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sequence specific quantitative pcr based reaction
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Sequence Specific Quantitative Pcr Based Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene polymerase chain reaction pcr based site directed mutagenesis
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Pcr Based Site Directed Mutagenesis, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Medigen Biotech polymerase chain reaction sequencing based typing kit
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Sequencing Based Typing Kit, supplied by Medigen Biotech, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman mgb probe based polymerase chain reaction pcr
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Taqman Mgb Probe Based Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman based real time reverse transcription polymerase chain reaction rt pcr assays
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Taqman Based Real Time Reverse Transcription Polymerase Chain Reaction Rt Pcr Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bioMerieux sequence based polymerase chain reaction
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Sequence Based Polymerase Chain Reaction, supplied by bioMerieux, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction sequence based typing kits
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Sequence Based Typing Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction based sequencing
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Based Sequencing, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene polymerase chain reaction based mutagenesis strategy
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Based Mutagenesis Strategy, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene exsite polymerase chain reaction based site directed mutagenesis kit
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Exsite Polymerase Chain Reaction Based Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green based quantitative pcr reactions
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Sybr Green Based Quantitative Pcr Reactions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Quanta Biosciences sybr green based real time pcr reaction kit
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Sybr Green Based Real Time Pcr Reaction Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher based multiplexed polymerase chain reaction pcr
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Based Multiplexed Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pfu polymerase based chain reaction
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Pfu Polymerase Based Chain Reaction, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Asuragen custom pcr polymerase chain reaction based assay
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Custom Pcr Polymerase Chain Reaction Based Assay, supplied by Asuragen, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies polymerase chain reaction pcr based site directed mutagenesis
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Pcr Based Site Directed Mutagenesis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories polymerase chain reaction pcr based technologies
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Pcr Based Technologies, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transnetyx polymerase chain reaction q pcr based technologies
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
    Polymerase Chain Reaction Q Pcr Based Technologies, supplied by Transnetyx, used in various techniques. Bioz Stars score: 88/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sysmex Inostics digital polymerase chain reaction based
    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic <t>DNA.</t> A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected <t>F0</t> parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p
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    RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic DNA. A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected F0 parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p

    Journal: bioRxiv

    Article Title: Non-photopic and photopic visual cycles differentially regulate immediate, early and late-phases of cone photoreceptor-mediated vision

    doi: 10.1101/2020.02.15.950915

    Figure Lengend Snippet: RPE-expressed rlbp1b is not required cone photopic vision in zebrafish at 5 dpf. A) CRISPR/Cas9 knockout strategy of RPE-expressed rlbp1b . Guides were targeted to exon 2 and exon 3 to induce a 1440 bp deletion in rlbp1b which contains 7 exons. Forward primer 1 (FP1) to reverse primer 1 (RP1) amplifies a 1688 bp wildtype product or 248 bp product when deletion is present. A poison forward primer (FP2) was designed in the middle of the deleted region to amplify a 469 bp wildtype product. B) An agarose gel (1.5%) depicting the presence of the deletion in individual F2 larval genomic DNA. A wildtype control was used to amplify the upper band (469 bp) and gDNA from the injected F0 parent fin was used as a positive control to amplify both upper and lower bands (mosaic). L = 100 bp ladder. C) Gene expression analysis of rlbp1b in rlbp1b −/− eyes. Data were analyzed by unpaired, two-tailed t-tests where **=p

    Article Snippet: Genomic DNA extraction and polymerase chain reaction DNA was extracted from injected F0 or germline F2 rlbp1b larvae by boiling the sample at 95°C in NaOH (50 mM) (Sigma Aldrich, UK) before neutralizing with 1/10th Trizma Base (Sigma Aldrich, UK).

    Techniques: CRISPR, Knock-Out, Agarose Gel Electrophoresis, Injection, Positive Control, Expressing, Two Tailed Test