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  • 99
    Thermo Fisher real time pcr one round t7 rna polymerase mediated linear amplification
    Real Time Pcr One Round T7 Rna Polymerase Mediated Linear Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore first round pcr protocol mineral oil
    First Round Pcr Protocol Mineral Oil, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc round pcr
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Pcr, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sangon Biotech first round pcr
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    First Round Pcr, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Macrogen second round pcr assays
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Second Round Pcr Assays, supplied by Macrogen, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher second round nested pcr
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Second Round Nested Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher first round pcr contained amplitaq gold 360 master mix
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    First Round Pcr Contained Amplitaq Gold 360 Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Genewiz second round pcr
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Second Round Pcr, supplied by Genewiz, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Illumina Inc pcr round 1
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Pcr Round 1, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Biomatters Ltd pcr round
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Pcr Round, supplied by Biomatters Ltd, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    STAB VIDA first round pcr primers
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    First Round Pcr Primers, supplied by STAB VIDA, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher first round pcr used reaction buffer
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    First Round Pcr Used Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sigma-Genosys first round pcr
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    First Round Pcr, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Eurofins round 2 arbitrary pcr reactions
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round 2 Arbitrary Pcr Reactions, supplied by Eurofins, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Sarstedt round bottom 96 well pcr plates
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Bottom 96 Well Pcr Plates, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Porvair Sciences round bottom pcr plates
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Bottom Pcr Plates, supplied by Porvair Sciences, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene round circle pcr based site directed mutagenesis kit
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Circle Pcr Based Site Directed Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene round circle pcr based site directed mutagenesis
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Circle Pcr Based Site Directed Mutagenesis, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher round pcr 1x pcr buffer
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Pcr 1x Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science round pcr mix contained myfi mix
    <t>Norovirus</t> GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round <t>PCR</t> was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.
    Round Pcr Mix Contained Myfi Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Norovirus GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round PCR was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.

    Journal: Viruses

    Article Title: Recombinant GII.P16/GII.4 Sydney 2012 Was the Dominant Norovirus Identified in Australia and New Zealand in 2017

    doi: 10.3390/v10100548

    Figure Lengend Snippet: Norovirus GII genotype distribution in wastewater samples collected from Sydney and Melbourne, 2017. The norovirus GII capsid genotypic distribution was determined in wastewater samples by amplicon sequencing using NGS technology. After amplification of the capsid region, a second round PCR was performed for the addition of adapters prior NGS library preparation. Libraries were sequenced on the MiSeq platform and an average of 152,879 reads were generated for each sample. Geneious was used for merging and mapping of the reads to the norovirus GII reference sequences. ( A ) The monthly genotype distribution of norovirus GII viruses in wastewater samples collected from Bondi WWTP in Sydney, Australia. ( B ) Norovirus GII genotypic diversity was examined in wastewater samples collected monthly from Malabar WWTP in Sydney, Australia. ( C ) The monthly norovirus GII genotype diversity was also examined in Melbourne, all samples were collected from western wastewater treatment plant. Samples were not collected between January and April 2017. Norovirus GII capsid genotype and GII.4 recombinants are labelled in different colors as indicated by the legend.

    Article Snippet: For wastewater samples, the 5′ end of norovirus GII capsid was amplified, followed by a second round PCR for the addition of universal sequencing adapters, following the manufacturer’s protocol (Illumina, San Diego, CA, USA).

    Techniques: Amplification, Sequencing, Next-Generation Sequencing, Polymerase Chain Reaction, Generated, Western Blot