pcr reaction mixture Search Results


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  • 99
    New England Biolabs phusion pcr mix
    Phusion Pcr Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcr
    Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 111176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polymerase chain reaction pcr mixture
    Polymerase Chain Reaction Pcr Mixture, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co polymerase chain reaction pcr mixture
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Polymerase Chain Reaction Pcr Mixture, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kaneka Corp standard polymerase chain reaction pcr mix
    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control <t>PCR</t> was performed under the same conditions except for without template <t>DNA</t> (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.
    Standard Polymerase Chain Reaction Pcr Mix, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polymerase chain reaction pcr mixture
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Polymerase Chain Reaction Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech polymerase chain reaction pcr mix kit
    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time <t>PCR</t> and the DNA-binding dye, <t>SYBR</t> green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).
    Polymerase Chain Reaction Pcr Mix Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meridian Life Science sensimix polymerase chain reaction mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Sensimix Polymerase Chain Reaction Mix, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybrgreen polymerase chain reaction pcr mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Sybrgreen Polymerase Chain Reaction Pcr Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr mixture
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polymerase chain reaction pcr nucleotide mix
    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by <t>PCR</t> using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M <t>DNA</t> marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.
    Polymerase Chain Reaction Pcr Nucleotide Mix, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcr reaction mixture
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Pcr Reaction Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 2570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa polymerase chain reaction pcr master mix
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Polymerase Chain Reaction Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction pcr
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioneer Corporation polymerase chain reaction pcr premix
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Polymerase Chain Reaction Pcr Premix, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr pcr polymerase chain reaction master mix
    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by <t>SYBR-Green</t> I real-time <t>PCR.</t> The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.
    Sybr Pcr Polymerase Chain Reaction Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche pcr reaction mixture
    KGFR and KGF in pancreatic cancer cell lines. A: <t>RT-PCR</t> analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. Total RNA was extracted from PANC-1; MIA PaCa-2; KLM-1; Capan-1; and PK-1, -8, -9, and -59 cells and used to perform <t>cDNA</t> synthesis and PCR analysis. KGFR mRNA (150 bp) was detected in all cell lines (top). KGF mRNA (163-bp) was detected in all pancreatic cancer cell lines, except for MIA PaCa-2 cells (middle). β-Actin mRNA (163 bp) served as a loading control (bottom). B and C: Q-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. KGFR mRNA was expressed at variable levels in all pancreatic cancer cell lines. KGF mRNA was not detected in MIA PaCa-2 cells and was expressed at variable levels in the other pancreatic cancer cell lines. Results are expressed as KGFR/β-actin and KGF/β-actin. Gene expression measurements were performed in triplicate. Bars represent the mean ± SE. D: Western blot analysis of KGFR in pancreatic cancer cell lines. A band corresponding to the 105-kd KGFR protein was detected in all pancreatic cancer cell lines (top). β-Actin served as a loading control (bottom). E: Immunofluorescent analysis of KGFR expression in MIA PaCa-2 cells. KGFR immunoreactivity was detected in the cytoplasm and/or on the membrane of cancer cells. In some cases, a strong KGFR signal was detected at the cell membrane ( arrowheads ). Bar = 50 μm.
    Pcr Reaction Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polymerase chain reaction pcr reaction mix
    KGFR and KGF in pancreatic cancer cell lines. A: <t>RT-PCR</t> analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. Total RNA was extracted from PANC-1; MIA PaCa-2; KLM-1; Capan-1; and PK-1, -8, -9, and -59 cells and used to perform <t>cDNA</t> synthesis and PCR analysis. KGFR mRNA (150 bp) was detected in all cell lines (top). KGF mRNA (163-bp) was detected in all pancreatic cancer cell lines, except for MIA PaCa-2 cells (middle). β-Actin mRNA (163 bp) served as a loading control (bottom). B and C: Q-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. KGFR mRNA was expressed at variable levels in all pancreatic cancer cell lines. KGF mRNA was not detected in MIA PaCa-2 cells and was expressed at variable levels in the other pancreatic cancer cell lines. Results are expressed as KGFR/β-actin and KGF/β-actin. Gene expression measurements were performed in triplicate. Bars represent the mean ± SE. D: Western blot analysis of KGFR in pancreatic cancer cell lines. A band corresponding to the 105-kd KGFR protein was detected in all pancreatic cancer cell lines (top). β-Actin served as a loading control (bottom). E: Immunofluorescent analysis of KGFR expression in MIA PaCa-2 cells. KGFR immunoreactivity was detected in the cytoplasm and/or on the membrane of cancer cells. In some cases, a strong KGFR signal was detected at the cell membrane ( arrowheads ). Bar = 50 μm.
    Polymerase Chain Reaction Pcr Reaction Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Arraystar polymerase chain reaction pcr master mix
    KGFR and KGF in pancreatic cancer cell lines. A: <t>RT-PCR</t> analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. Total RNA was extracted from PANC-1; MIA PaCa-2; KLM-1; Capan-1; and PK-1, -8, -9, and -59 cells and used to perform <t>cDNA</t> synthesis and PCR analysis. KGFR mRNA (150 bp) was detected in all cell lines (top). KGF mRNA (163-bp) was detected in all pancreatic cancer cell lines, except for MIA PaCa-2 cells (middle). β-Actin mRNA (163 bp) served as a loading control (bottom). B and C: Q-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. KGFR mRNA was expressed at variable levels in all pancreatic cancer cell lines. KGF mRNA was not detected in MIA PaCa-2 cells and was expressed at variable levels in the other pancreatic cancer cell lines. Results are expressed as KGFR/β-actin and KGF/β-actin. Gene expression measurements were performed in triplicate. Bars represent the mean ± SE. D: Western blot analysis of KGFR in pancreatic cancer cell lines. A band corresponding to the 105-kd KGFR protein was detected in all pancreatic cancer cell lines (top). β-Actin served as a loading control (bottom). E: Immunofluorescent analysis of KGFR expression in MIA PaCa-2 cells. KGFR immunoreactivity was detected in the cytoplasm and/or on the membrane of cancer cells. In some cases, a strong KGFR signal was detected at the cell membrane ( arrowheads ). Bar = 50 μm.
    Polymerase Chain Reaction Pcr Master Mix, supplied by Arraystar, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene qpcr master mix
    KGFR and KGF in pancreatic cancer cell lines. A: <t>RT-PCR</t> analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. Total RNA was extracted from PANC-1; MIA PaCa-2; KLM-1; Capan-1; and PK-1, -8, -9, and -59 cells and used to perform <t>cDNA</t> synthesis and PCR analysis. KGFR mRNA (150 bp) was detected in all cell lines (top). KGF mRNA (163-bp) was detected in all pancreatic cancer cell lines, except for MIA PaCa-2 cells (middle). β-Actin mRNA (163 bp) served as a loading control (bottom). B and C: Q-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. KGFR mRNA was expressed at variable levels in all pancreatic cancer cell lines. KGF mRNA was not detected in MIA PaCa-2 cells and was expressed at variable levels in the other pancreatic cancer cell lines. Results are expressed as KGFR/β-actin and KGF/β-actin. Gene expression measurements were performed in triplicate. Bars represent the mean ± SE. D: Western blot analysis of KGFR in pancreatic cancer cell lines. A band corresponding to the 105-kd KGFR protein was detected in all pancreatic cancer cell lines (top). β-Actin served as a loading control (bottom). E: Immunofluorescent analysis of KGFR expression in MIA PaCa-2 cells. KGFR immunoreactivity was detected in the cytoplasm and/or on the membrane of cancer cells. In some cases, a strong KGFR signal was detected at the cell membrane ( arrowheads ). Bar = 50 μm.
    Qpcr Master Mix, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene pcr mix
    A2A activation affects the action of PTX in attenuating lung injury. Rats were pre-administered with vehicle, PTX (50 mg/Kg), PTX + ZM-241385 (ZM) or PTX + CGS-21680 (CGS), and then underwent IAC. A. The expression of A2A receptor mRNA was examined by <t>qRT-PCR,</t> and the level of mRNA from sham-operated rats was defined as 1. B. The expression of A2A receptor protein was detected by Western blot analysis. The density of each band was normalized to GAPDH. C. MPO activity in lung tissues was measured. D. Protein concentrations in BAL fluids were measured. E. Cell numbers in BAL fluids were counted. F. Numbers of neutrophils, macrophages/monocytes and lymphocytes in BAL fluids were counted. *P
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    Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Journal: BioMed Research International

    Article Title: Population Abundance of Potentially Pathogenic Organisms in Intestinal Microbiome of Jungle Crow (Corvus macrorhynchos) Shown with 16S rRNA Gene-Based Microbial Community Analysis

    doi: 10.1155/2013/438956

    Figure Lengend Snippet: Amplified partial fragment of 16S rRNA gene from crow 1 (A), crow 2 (B), and crow 3 (C) for evaluation of intestinal microbiomes. Control PCR was performed under the same conditions except for without template DNA (D). Molecular size marker (M) shows 1.5, 1.0, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, and 0.1 kb from top to bottom.

    Article Snippet: PCR Conditions Twenty-five to fifty nanograms of isolated DNA were transferred to a PCR tube containing 50 μ L of a polymerase chain reaction (PCR) mixture containing LA Taq DNA polymerase (TaKaRa Bio, Shiga, Japan).

    Techniques: Amplification, Polymerase Chain Reaction, Marker

    miR-92a-3p downregulates NF2 mRNA and protein expression by interacting with its conserved MRE within the NF2 −3′UTR. (A) Semi-quantitative reverse transcription-polymerase chain reaction (semi-RT-qPCR), (B) quantitative RT-PCR (RT-qPCR) and (C) western blotting detection of NF2 /Merlin expression levels in HCT116 cells transfected with empty vector or miR-92a expression construct. (D) RT-qPCR and (E) western blotting detection of NF2 /Merlin expression in HCT116 cells co-transfected with pmR-ZsGreen1 and negative control target protector (TPneg), or with miR-92a expression construct and miR-92a-target protector (TP-92a). All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Journal: Oncology Reports

    Article Title: MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells

    doi: 10.3892/or.2019.7020

    Figure Lengend Snippet: miR-92a-3p downregulates NF2 mRNA and protein expression by interacting with its conserved MRE within the NF2 −3′UTR. (A) Semi-quantitative reverse transcription-polymerase chain reaction (semi-RT-qPCR), (B) quantitative RT-PCR (RT-qPCR) and (C) western blotting detection of NF2 /Merlin expression levels in HCT116 cells transfected with empty vector or miR-92a expression construct. (D) RT-qPCR and (E) western blotting detection of NF2 /Merlin expression in HCT116 cells co-transfected with pmR-ZsGreen1 and negative control target protector (TPneg), or with miR-92a expression construct and miR-92a-target protector (TP-92a). All experiments were performed in cells maintained in 0.5% serum. Data presented are representative of three independent trials and expressed as the mean ± standard deviation (SD). *P≤0.05, **P≤0.01. NF2 , neurofibromin 2; UTR, untranslated region; MRE, microRNA response element.

    Article Snippet: The 3′UTR of human NF2 isoform I (NM_000268.3) and the pre-miR-92a-1 gene (NR_029508.1) were amplified in a polymerase chain reaction (PCR) reaction mixture containing a final concentration of 1X PCR buffer (Titanium® Taq PCR buffer; Clontech Laboratories, Inc., Mountain View, CA, USA), 0.125 µM of each deoxynucleoside triphosphate (dNTPs) (Promega Corporation, Madison, WI, USA), 2 µM each of the forward and reverse primers, 1X Taq polymerase (Titanium® Taq polymerase; Clontech Laboratories, Inc.) and wild-type human genomic DNA template available in the Disease Molecular Biology and Epigenetics Laboratory of the National Institute of Molecular Biology and Biotechnology (DMBEL-NIMBB).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Transfection, Plasmid Preparation, Construct, Negative Control, Standard Deviation

    Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).

    Journal: Molecular Vision

    Article Title: Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

    doi:

    Figure Lengend Snippet: Regulation of choroidal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew choroid using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 1 and 5 day CHRM2 gene data, all other data n=6).

    Article Snippet: Total RNA (0.5 μg) was reverse transcribed as described above, and amplifications were performed using a commercial PCR mix incorporating SYBR green 1 (FastStart DNA Master Mix; Roche).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, SYBR Green Assay

    Regulation of retinal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew retina using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=6).

    Journal: Molecular Vision

    Article Title: Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

    doi:

    Figure Lengend Snippet: Regulation of retinal muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew retina using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=6).

    Article Snippet: Total RNA (0.5 μg) was reverse transcribed as described above, and amplifications were performed using a commercial PCR mix incorporating SYBR green 1 (FastStart DNA Master Mix; Roche).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, SYBR Green Assay

    Regulation of scleral muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew sclera using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 5 day myopia data, 1 day myopia and normal data, n=6).

    Journal: Molecular Vision

    Article Title: Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia

    doi:

    Figure Lengend Snippet: Regulation of scleral muscarinic receptor subtype gene expression during myopia development. The regulation of the five mAChR s was assessed in the tree shrew sclera using real-time PCR and the DNA-binding dye, SYBR green I. Data was calculated relative to the housekeeping gene, HPRT , and expressed relative to the contralateral control eye (for myopic groups) or left/right eyes (for normal groups). Data are shown as the mean percentage change±SEM (n=5 for 5 day myopia data, 1 day myopia and normal data, n=6).

    Article Snippet: Total RNA (0.5 μg) was reverse transcribed as described above, and amplifications were performed using a commercial PCR mix incorporating SYBR green 1 (FastStart DNA Master Mix; Roche).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Binding Assay, SYBR Green Assay

    Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Expression of tooth-related genes in the primary and immortalized dental papilla mesenchymal cells. ( A ) Total RNAs from the primary and immortalized cells were extracted and reversely transcribed. The cDNAs were amplified by PCR using specific primers shown in Table 1 . The PCR products were run on 1% agarose gels and stained with ethidium bromide. M DNA marker (Low DNA mass ladder, Invitrogen), Con negative control. ( B ) Immunohistochemistry staining with antibodies against Col1a1, Dlx3, Dmp1, Dsp, Oc, Opn, Osx, and Runx2 in the primary and immortalized murine dental papilla mesenchymal cells. Scale bar = 10 μM. ( C ). Expression of Dsp and Dmp1 proteins in the primary and immortalized mouse dental papilla mesenchymal cells was analyzed by Western blot assay using anti-Dsp and anti-Dmp1 antibodies. Two major fragments of Dmp1 were observed as 57- and 37-kDa, respectively, in a SDS-PAGE gele using anti-Dmp1 antibody. Multiple fragments of Dsp polypeptides were detected in MD10-F2 cells using anti-Dsp antibodiy. Major bands are approximately 120, 65, 60, and 40 kDa as indicated by arrows . β-actin was used as an internal control. Pri and Imm represent the primary and immoratalized dental papilla mesenchymal cells.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Expressing, Amplification, Polymerase Chain Reaction, Staining, Marker, Negative Control, Immunohistochemistry, Western Blot, SDS Page

    Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Journal: In Vitro Cellular & Developmental Biology. Animal

    Article Title: Immortalized mouse dental papilla mesenchymal cells preserve odontoblastic phenotype and respond to bone morphogenetic protein 2

    doi: 10.1007/s11626-013-9641-1

    Figure Lengend Snippet: Identification of SV40 transformation. ( A ) Genomic DNA in the primary and immortalized dental papilla mesenchymal cells was isolated and amplified by the SV40 specific primer. PCR products were run on an agarose gel and stained with ethidium bromide. Lane 1 , DNA marker (Low DNA mass ladder, Invitrogen); lane 2 , control; lane 3 , primary dental papilla mesenchymal cells; lane 4 , immortalized cells. ( B , C ) Immunohistochemical staining with antibody against simian virus 40 large T-antigen (SV40 T-Ag) in iMDP-3 cells and immunolabeling of SV40 protein was mostly present in the nucleus ( green color ; B ) whereas immunostaining was not seen in the primary cells ( C ). The cells were stained with DAPI for the nucleus. Con control, Pri primary, Imm immortalized.

    Article Snippet: Two-hundred nanograms of DNA (for pSV3 neo plasmid DNA 10 ng) were diluted in a 25-μl polymerase chain reaction (PCR) mix (Sigma–Aldrich).

    Techniques: Transformation Assay, Isolation, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Marker, Immunohistochemistry, Immunolabeling, Immunostaining

    Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Journal: Oncology Letters

    Article Title: Expression of miR-21, miR-31, miR-96 and miR-135b is correlated with the clinical parameters of colorectal cancer

    doi: 10.3892/ol.2012.714

    Figure Lengend Snippet: Standard curves were created with 10-fold serially diluted plasmid DNA containing (A) miR-21, (B) miR-31, (C) miR-96, (D) miR-135b or (E) U6 by SYBR-Green I real-time PCR. The Ct values obtained from the real-time PCR assays were plotted against the initial plasmid DNA copy number. The curves demonstrated a wide linear range: 10 1 –10 8 copies for miR-21, 10 2 –10 9 copies for miR-31, 10 2 –10 8 copies for miR-96 and miR-135b and 10 1 –10 8 copies for U6 snRNA. Correlation coefficients were > 0.996, and the melting-curves of miR-21, miR-31, miR-96, miR-135b and U6 are shown as a single, sharply-narrow peak, indicating that pure, homogeneous qPCR products were produced.

    Article Snippet: The 25 μl PCR reaction mixture included 1× SYBR premix Ex Taq mix (Takara), 2 μl RT products and 10 nM of each forward and reverse primer.

    Techniques: Plasmid Preparation, SYBR Green Assay, Real-time Polymerase Chain Reaction, Produced

    KGFR and KGF in pancreatic cancer cell lines. A: RT-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. Total RNA was extracted from PANC-1; MIA PaCa-2; KLM-1; Capan-1; and PK-1, -8, -9, and -59 cells and used to perform cDNA synthesis and PCR analysis. KGFR mRNA (150 bp) was detected in all cell lines (top). KGF mRNA (163-bp) was detected in all pancreatic cancer cell lines, except for MIA PaCa-2 cells (middle). β-Actin mRNA (163 bp) served as a loading control (bottom). B and C: Q-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. KGFR mRNA was expressed at variable levels in all pancreatic cancer cell lines. KGF mRNA was not detected in MIA PaCa-2 cells and was expressed at variable levels in the other pancreatic cancer cell lines. Results are expressed as KGFR/β-actin and KGF/β-actin. Gene expression measurements were performed in triplicate. Bars represent the mean ± SE. D: Western blot analysis of KGFR in pancreatic cancer cell lines. A band corresponding to the 105-kd KGFR protein was detected in all pancreatic cancer cell lines (top). β-Actin served as a loading control (bottom). E: Immunofluorescent analysis of KGFR expression in MIA PaCa-2 cells. KGFR immunoreactivity was detected in the cytoplasm and/or on the membrane of cancer cells. In some cases, a strong KGFR signal was detected at the cell membrane ( arrowheads ). Bar = 50 μm.

    Journal: The American Journal of Pathology

    Article Title: Enhanced Expression of Keratinocyte Growth Factor and Its Receptor Correlates with Venous Invasion in Pancreatic Cancer

    doi: 10.2353/ajpath.2007.060935

    Figure Lengend Snippet: KGFR and KGF in pancreatic cancer cell lines. A: RT-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. Total RNA was extracted from PANC-1; MIA PaCa-2; KLM-1; Capan-1; and PK-1, -8, -9, and -59 cells and used to perform cDNA synthesis and PCR analysis. KGFR mRNA (150 bp) was detected in all cell lines (top). KGF mRNA (163-bp) was detected in all pancreatic cancer cell lines, except for MIA PaCa-2 cells (middle). β-Actin mRNA (163 bp) served as a loading control (bottom). B and C: Q-PCR analysis of KGFR and KGF mRNA in pancreatic cancer cell lines. KGFR mRNA was expressed at variable levels in all pancreatic cancer cell lines. KGF mRNA was not detected in MIA PaCa-2 cells and was expressed at variable levels in the other pancreatic cancer cell lines. Results are expressed as KGFR/β-actin and KGF/β-actin. Gene expression measurements were performed in triplicate. Bars represent the mean ± SE. D: Western blot analysis of KGFR in pancreatic cancer cell lines. A band corresponding to the 105-kd KGFR protein was detected in all pancreatic cancer cell lines (top). β-Actin served as a loading control (bottom). E: Immunofluorescent analysis of KGFR expression in MIA PaCa-2 cells. KGFR immunoreactivity was detected in the cytoplasm and/or on the membrane of cancer cells. In some cases, a strong KGFR signal was detected at the cell membrane ( arrowheads ). Bar = 50 μm.

    Article Snippet: PCR reaction mixture containing 2 μl of template cDNA, 3 mmol/L MgCl2 , and 0.5 μmol/L of primers, and LightCycler-FastStart DNA Master SYBR Green I mix was applied into a capillary tube (Roche).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Expressing, Western Blot

    A2A activation affects the action of PTX in attenuating lung injury. Rats were pre-administered with vehicle, PTX (50 mg/Kg), PTX + ZM-241385 (ZM) or PTX + CGS-21680 (CGS), and then underwent IAC. A. The expression of A2A receptor mRNA was examined by qRT-PCR, and the level of mRNA from sham-operated rats was defined as 1. B. The expression of A2A receptor protein was detected by Western blot analysis. The density of each band was normalized to GAPDH. C. MPO activity in lung tissues was measured. D. Protein concentrations in BAL fluids were measured. E. Cell numbers in BAL fluids were counted. F. Numbers of neutrophils, macrophages/monocytes and lymphocytes in BAL fluids were counted. *P

    Journal: American Journal of Translational Research

    Article Title: Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A

    doi:

    Figure Lengend Snippet: A2A activation affects the action of PTX in attenuating lung injury. Rats were pre-administered with vehicle, PTX (50 mg/Kg), PTX + ZM-241385 (ZM) or PTX + CGS-21680 (CGS), and then underwent IAC. A. The expression of A2A receptor mRNA was examined by qRT-PCR, and the level of mRNA from sham-operated rats was defined as 1. B. The expression of A2A receptor protein was detected by Western blot analysis. The density of each band was normalized to GAPDH. C. MPO activity in lung tissues was measured. D. Protein concentrations in BAL fluids were measured. E. Cell numbers in BAL fluids were counted. F. Numbers of neutrophils, macrophages/monocytes and lymphocytes in BAL fluids were counted. *P

    Article Snippet: The qRT-PCR reaction mixtures containing cDNA, PCR mix and A2A primers (5’-GAAGCAGATGGAGAGCCAAC-3’ and 5’-GAGAGGATGATGGCCAGGTA-3’) were prepared, and analyzed by MX3000P Real-time PCR systems (Stratagen, USA).

    Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Western Blot, Activity Assay